tnfα il 6 mip 1α il 1β aβ42 and tau ps199 elisa

báo cáo hóa học: " Sinomenine inhibits microglial activation by Ab and confers neuroprotection" doc

Ngày tải lên : 19/06/2014, 22:20

... sinomenine, was used to assay levels of IL- 6, IL- 10, IL- 12, TNF-a, IFN-g and MCP-1 Statistically significant increases in levels of IL- 6, TNF-a, MCP-1 and IL- 12 were observed following treatment ... TNF-a and IL- 6, n = for MCP-1) *, p < 0.05 and #, p < 0. 061 Control ADDL in all groups) Collectively, these results show that sinomenine reduces oligomeric Ab-induced release of inflammatory and ... = 1.1 ± 0. 16, p > 0 .6 compared to control; ADDL + sinomenine = 1. 16 ± 0.08, p > 0.43 compared to ADDL; IL- 10, ADDL = 1.28 ± 0.2, p > 0.30 compared to control; ADDL + sinomenine = 1. 16 ± 0.12,...

11
343
0

Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx

Ngày tải lên : 22/02/2014, 04:20

... RRR-a-tocopheryl succinate-induced apoptosis of human breast cancer cells Cancer Res 61 , 65 69– 65 76 16 Neuzil, J., Weber, T., Terman, A., Weber, C & Brunk, U.T (2001) Vitamin E analogues as inducers ... production (Eur J Biochem 269 ) 2 369 Fig Enhancements by TS of LPS/IFN-induced NO production (A) and iNOS induction (B) in VSMC The concentrations of LPS and IFN were 10 lgÆmL)1 and 100 UÆmL)1, respectively ... lipopolysaccharide-induced nitric oxide synthase expression in RAW 264 .7 macrophages: Involvement of a nuclear factor kB-dependent mechanism J Immunol 161 , 62 06 62 14 31 Chen, C.-C., Chiu, K.-T., Sun, Y.-T & Chen,...

6
494
0

Báo cáo khoa học: Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro doc

Ngày tải lên : 07/03/2014, 15:20

... of the Agilent 7500c ICPMS system The isotopes 64 ,66 Zn and 111,114Cd were monitored All measurements were performed at least twice and the averages were taken over both isotopes of zinc and cadmium, ... gel-ﬁltration step Dilution effects are partly responsible for slight differences in both these UV traces as well as 66 Zn and 114Cd intensities between the pure MT2 sample and mixtures with DEA/NO and GSH ... cysteine, glutathione and cysteine-containing proteins: implications for in vivo S-nitrosation J Chem Soc Perkin Trans I 2, 66 3 66 9 Stamler, J.S (1994) Redox signaling: nitrosylation and related target...

9
398
0

Báo cáo khoa học: A pathway through interferon-c is the main pathway for induction of nitric oxide upon stimulation with bacterial lipopolysaccharide in mouse peritoneal cells pot

Ngày tải lên : 17/03/2014, 10:20

... RAW 264 .7 and J774.1 Production of IFN-c and IL- 12 by murine PEC upon LPS stimulation We then investigated the production of IFN-c and related cytokines upon LPS stimulation of PEC and RAW 264 .7 ... of the adherent cells and RAW 264 .7 cells to produce NO was strong, but the response to produce IL- 6 and TNF-a was not observed (data not shown), similarly to the case of IL- 12p70 These results ... cells and the subsequent eﬀect on the production of NO (A), IFN-c (B) and IL- 12 (C) in response to stimulation with LPS, IFN-c and IL- 12 + IL- 18 PEC of C3H/HeN mice were cultured for h and the...

10
395
0

Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot

Ngày tải lên : 30/03/2014, 04:20

... ER, Billecke SS & Osawa Y (2004) Ubiquitination and degradation of neuronal nitric-oxide synthase in vitro: dimer stabilization protects the enzyme from proteolysis Mol Pharmacol 66 , 964 – 969 12 ... and HMS rats, were submitted to 8% SDS-PAGE, followed by immunoblotting revealed with serum-l-calpain mAb 56. 3 [ 36] and monoclonal IgG milli-calpain The immunoreactive material was detected and ... eNOS, iNOS and HSP90 antibodies were purchased from BD Transduction Laboratories, Milan, Italy b-Actin and milli-calpain antibodies were obtained from Sigma Aldrich, Milan, Italy Desmin and talin...

11
344
0

báo cáo hóa học: " Signaling pathways mediating a selective induction of nitric oxide synthase II by tumor necrosis factor alpha in nerve growth factor-responsive cells" pptx

Ngày tải lên : 19/06/2014, 22:20

... Activity-dependent mobilization of the adhesion Page 12 of 13 (page number not for citation purposes) Journal of Neuroinflammation 2005, 2:19 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 ... association with Hirano bodies, neurofibrillary tangles, and senile plaques Journal of Neuropathology and Experimental Neurology 1999, 58:1 163 -1 169 Darville MI, Eizirik DL: Regulation by cytokines ... collected and protein content was measured using the standard BCA protein assay (Pierce, Rockford, IL, USA) Protein extracts (40 µg) were diluted in 6X sample buffer and loaded onto a 6% SDS-polyacrylamide...

13
447
0

Báo cáo khoa học: "Enhanced expression of constitutive and inducible forms of nitric oxide synthase in autoimmune encephalomyelitis" pdf

Ngày tải lên : 07/08/2014, 14:22

... Br J Pharmacol 1992, 1 06( 4), 766 - 767 Choi, C., Park, J Y., Lee, J., Lim, J H., Shin, E C., Ahn, Y S., Kim, C H., Kim, S J., Kim, J D., Choi, I S and Choi, I H Fas ligand and Fas are expressed ... in human astrocytes and the expression increases with IL- 1, IL- 6, TNF-alpha or IFN-gamma J Immunol 1999, 162 (4), 1889-1895 10 Cowden, W B., Cullen, F A., Staykova, M A and Willenborg, D O Nitric ... magnification: A, × 33; B and C, ×132 A: TUNEL and hematoxylin, B: TUNEL and either rabbit anti-GFAP, C: TUNEL and rabbit and ED1 Immunohistochemical localization of nNOS, eNOS, and iNOS in EAE In the...

7
290
0

Báo cáo khoa học: "Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development" ppsx

Ngày tải lên : 07/08/2014, 18:21

... oocytes of unilaminar and multilaminar follicles, and to granulosa and theca cells However, it was previously reported that iNOS, particularly in large follicles, was localized to the oocytes and cumulus ... stimulation in vivo J Clin Invest 1995, 96, 2719-27 26 15 Xie QW, Nathan C The high-output nitric oxide pathway: role and regulation J Leukoc Biol 1994, 56, 5 76- 582 16 Zackrisson U, Mikuni M, Wallin A, ... µm; in E-J, 60 µm the ovarian follicles Unilaminar follicles, multilaminar follicles, and antral follicles were seen in the cortex (Fig 1A) From the interior to exterior, the multilaminar follicle...

5
245
0

$Báo cáo y học: "Protective effects of total fraction of avocado/soybean unsaponifiables on the structural changes in experimental dog osteoarthritis: inhibition of nitric oxide synthase and matrix metalloproteinase-13" docx$

Báo cáo y học: "Protective effects of total fraction of avocado/soybean unsaponifiables on the structural changes in experimental dog osteoarthritis: inhibition of nitric oxide synthase and matrix metalloproteinase-13" docx

Ngày tải lên : 09/08/2014, 14:20

... [4] They can also inhibit the stimulating action of IL- 1β on stromelysin and collagenase and inhibit production of IL- 6, IL- 8 and prostaglandin E2 [5] In addition, it was demonstrated that ASU ... appellation Osteoarthritis Cartilage 2008, 16: 1275-12 76 Dougados M: Symptomatic slow-acting drugs for osteoarthritis: what are the facts? Joint Bone Spine 20 06, 73 :60 6 -60 9 Mauviel A, Daireaux M, ... ADAMTS5 is the major aggrecanase in mouse cartilage in vivo and in vitro Nature 2005, 434 :64 8 -65 2 26 Boileau C, Martel-Pelletier J, Fahmi H, Mineau F, Boily M, Pelletier JP: The peroxisome proliferator-activated...

9
547
0

Báo cáo y học: "Suppression of nitric oxide production from nasal fibroblasts by metabolized clarithromycin in vitro" potx

Ngày tải lên : 11/08/2014, 03:20

... Eur Respir J 2004, 23 :67 1 -67 8 Nadel JA: Inflammation and asthma J Allergy Clin Immunol 1984, 73 :65 1 -65 3 Muranaka H, Suga M, Sato K, Nakagawa K, Akaike T, Okamoto T, Maeda H, Ando M: Superoxide scavenging ... The antioxidant, taurine reduced lopopolysaccharide (LPS)-induced generation of ROS, and activation of MAPKs and Bax in cultured pneumocytes Pul Pharmacol Ther 2009, 22: 562 - 566 40 Kan H, Xie Z, ... suppressed by CAM and M-4, when these agents were added to cell cultures at 0.4 μg/ml and 0.04 μg/ml, respectively (Figure 6B) Influence of CAM and M-4 on iNOS mRNA expression Assay for CAM and M-4 on...

12
376
0

Báo cáo y học: "Suppression of nitric oxide production from nasal fibroblasts by metabolized clarithromycin in vitro" pptx

Ngày tải lên : 11/08/2014, 06:22

... Eur Respir J 2004, 23 :67 1 -67 8 Nadel JA: Inflammation and asthma J Allergy Clin Immunol 1984, 73 :65 1 -65 3 Muranaka H, Suga M, Sato K, Nakagawa K, Akaike T, Okamoto T, Maeda H, Ando M: Superoxide scavenging ... The antioxidant, taurine reduced lopopolysaccharide (LPS)-induced generation of ROS, and activation of MAPKs and Bax in cultured pneumocytes Pul Pharmacol Ther 2009, 22: 562 - 566 40 Kan H, Xie Z, ... suppressed by CAM and M-4, when these agents were added to cell cultures at 0.4 μg/ml and 0.04 μg/ml, respectively (Figure 6B) Influence of CAM and M-4 on iNOS mRNA expression Assay for CAM and M-4 on...

12
301
0

Báo cáo y học: " Central role of nitric oxide in the pathogenesis of rheumatoid arthritis and systemic lupus erythematosus" potx

Ngày tải lên : 12/08/2014, 14:22

... co-expression of inducible nitric Page of 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 oxide synthase and protein kinase C-eta in peripheral blood monocytederived macrophages ... known as Th17 cells Th17 cells have been identified as cells induced by IL- 6 and TGF-β and expanded by IL- 23 [65 ] Similarly to Th1 and Th2 subsets, Th17 development relies on the action of a lineage-specific ... handled by either Th1 or Th2 cells, and have also been strongly implicated in allergic diseases [66 ] In addition to IL- 17, Th17 cells produce other proinflammatory cytokines such as IL- 21 and IL- 22...

6
295
0

Báo cáo y học: "Involvement of nitric oxide (NO) in cough reflex sensitivity between non-sensitized and OVA-sensitized guinea pigs" pptx

Ngày tải lên : 13/08/2014, 10:20

... 5 .6 1.8 ± 0.8 16. 8 ± 5 .6 ONO1714: 0.1 mg/kg 1.09 ± 0.12 74.5 ± 4.3 6. 2 ± 1.5 1.4 ± 0.4 17.9 ± 4.7 ONO1714: 0.3 mg/kg 0.88 ± 0.09 76. 0 ± 2.1 5.3 ± 3.3 1.5 ± 0.5 17.7 ± 1 .6 Control 2 .63 ± 0. 26 ... 24.1 ± 3.1 2.5 ± 0 .6 0.9 ± 0.3 72.4 ± 3.1 ONO1714: 0.1 mg/kg 2 .68 ± 0.12 25.1 ± 1.0 2.8 ± 0.3 0.5 ± 0.2 71 .6 ± 1.4 ONO1714: 0.3 mg/kg 2 .64 ± 0. 36 28.5 ± 3 .6 0.8 ± 0.3 0.8 ± 0.3 69 .9 ± 3.7 Total ... prepare 10 -6 and 10-4 M solutions Each conscious guinea pig was placed in an airtight, custom-built, transparent plastic box consisting of two isolated chambers: a head chamber ( 160 0 mL volume) and...

9
306
0

Báo cáo y học: "Time course of nitric oxide synthases, nitrosative stress, and poly(ADP ribosylation) in an ovine sepsis model" pot

Ngày tải lên : 13/08/2014, 21:20

... Shock 19 96, 6: 79-88 12 Szabo C, Ischiropoulos H, Radi R: Peroxynitrite: biochemistry, pathophysiology and development of therapeutics Nat Rev Drug Discov 2007, 6: 662 -68 0 13 Szabo C: DNA strand breakage ... metabolism, and inflammation Time after injury (hours) Control 12 18 24 MAP, mmHg 105 ± 108 ± 10 87 ± 4a 81 ± 4a 76 ± 11b 63 ± 2c CO, L/min 3.8 ± 0.2 5.4 ± 0.4 5.4 ± 0.1 6. 6 ± 0.6a 6. 9 ± 0.5a ... oxygenation and shunting The ovine model of ALI and sepsis induced by smoke inhalation and instillation of Pseudomonas aeruginosa into the lungs has been previously described in detail [8,9] In...

10
247
0

The role of nitric oxide and other gaseous mediators in cardiovascular disease models; emphasis on septic shock

Ngày tải lên : 14/09/2015, 14:16

... Powerlab’s pressure transducer Figure 4 -6 Representative sodium nitrite standard curve Figure 4-7a Representative TNF-α standard curve Figure 4-7b Representative IL- 1β standard curve Figure 4-8a Graph ... 6- 5b Quantitation of liver (shown in Figure 6- 5a) CBS and CSE Figure 6- 6 Effect of flurbiprofen or nitroflurbiprofen on NF-κB activation in the liver of rats at h after LPS injection Figure 6- 7 ... (Minamiya et al., 1995) Besides TNF and ROS, other inducers of apoptosis have been shown to include steroids, IL- 1, IL- 6, IL- 10, FasL, heat shock, nitric oxide (NO) and FasLexpressing cytotoxic T...

222
297
0

The role of nitric oxide and prostaglandin e2 in prolonged hemorrhagic shock 2

Ngày tải lên : 16/09/2015, 15:54

... rats 261 .66 75.14 86. 95 3.25 Untreated rats *8 16. 82 45.97 *148.42 2. 36 Creatinine and GOT levels in different groups of rats Untreated rats showed significant difference (p

138
423
0

The role of nitric oxide and prostaglandin e2 in prolonged hemorrhagic shock 1

Ngày tải lên : 16/09/2015, 15:54

... different 60 groups of rats Figure 4.3F Plasma GOT levels at 24, 48 and 72 hours in different 60 groups of rats Figure 4.4.1 Hemotoxylin and eosin stain of Kidneys 62 Figure 4.4.2 Hemotoxylin and eosin ... Lungs 63 Figure 4.4.3 Hemotoxylin and eosin stain of Livers 64 Figure 4.4.4 Hemotoxylin and eosin stain of Cerebral cortex 65 Figure 4.5 Neurons in the cerebral cortex of the brain in sham- 67 operated ... FPI+HS, all treated groups showed significant 96 difference in nitrate/nitrite levels when compared with all treated rats in FPI and HS Figure 6. 6 AG and L-NAME treated rats showed significantly...

16
212
0

Role of nitric oxide in wound healing facilitatory effects of nitrosoglutathione a nitric oxide donor on the extracellular matrix deposition characteristics of wound healing

Ngày tải lên : 17/09/2015, 17:19

... Nitrosothiols 60 1.5.3.4 Interaction of Nitric Oxide with antioxidants 2.0 58 60 Hypothesis 62 iv Contents Page Materials and Methods 65 3.0 Materials and Methods 66 3.1 Materials 66 3.1.1 Chemicals and ... and reagents 66 3.1.1.1 Anaesthetic agents 66 3.1.1.2 General Chemicals 66 3.1.1.3 Other Chemicals 67 3.1.1.4 Biological products 67 3.1.1.5 Commercial kits 68 3.1.1 .6 Instruments 68 3.1.2 Experimental ... Activity 1 16 4 .6. 1.1 Effects of GSNO, SNAP and GSH on scar nitrite content 1 16 4 .6. 1.2 Effects of AG on scar nitrite content 117 4 .6. 2 Plasma nitrite content 118 4 .6. 2.1 Effects of GSNO and SNAP...

258
672
0

Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"

Ngày tải lên : 31/10/2012, 15:12

... Gynecologica Scandinavica 2005; 84 :67 9 -68 9 Tsujimoto Y., Shimizu S Bcl-2 family:life-or-death switch FEBS Lett., 2000; 466 : 6- 10 Nagata S Apoptosis by death factor Cell 1997; 88: 355- 365 Yoo G.H., ... GST-p, Hsp70 -64 kD, Bcl-x-22kD, PTEN, and caspase-7 showed 3.75-fold, 3.31-fold, 2 .66 -fold, 2.32-fold, 1.91-fold, and 1. 86- fold increases of protein expression, respectively On the other hand, smac/DIABLO, ... PowerBlot Protein Hsp70 -64 kD Caspase -6 Confidence level 3 3 3 (-) Under (+) Over + + + + + + + Fold change 2.32 3.75 1. 86 3.31 1.91 3.55 2 .66 0/+ JNK-phospho-specific - 2. 06 Bcl-x Caspase-3 Caspase-7...

9
499
0

Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf

Ngày tải lên : 14/02/2014, 22:20

... ratio of 6. 6 : 1) The solution was left for 30 at 50 °C, and diluted with DMEM, giving the desired concentrations Cell culture and treatment Human hepatocellular HepG2 (ATCC, HB-8 065 ) and Huh7 ... 2 76, 38 061 –38 067 30 Goldberg EM, Lester DS, Borchardt DB & Zidovetzki R (1994) Effects of diacylglycerols and Ca2+ on structure of phosphatidylcholine ⁄ phosphatidylserine bilayers Biophys J 66 , ... Bim and PUMA up-regulation and hepatocyte lipoapoptosis J Hepatol 52, 5 86 593 Borradaile NM, Han X, Harp JD, Gale SE, Ory DS & Schaffer JE (20 06) Disruption of endoplasmic reticulum structure and...

12
721
0