tiền xử lý bột nấm linh chi bằng sóng siêu âm và enzyme papain
Folk medicine cratoxylum cochinchinense antioxidant but cytotoxic
... (Cratoxylum cochinchinense, Cortex magnoliae officinalis, Psoralea corylifolia L, Curculigo orchioides Gaertn, and Glycyrrhiza uralensis Fisch.) were selected for further characterization C cochinchinense ... red TCM-traditional Chinese medicines TEAC-Trolox equivalents antioxidant capacity tR-retention time YCT-Cratoxylum cochinchinense extract Keywords⎯TCM, Cratoxylum cochinchinense, mangiferin, ... University of Singapore for supplying us the Chinese medicinal extracts This project would not be possible without these extracts, especially Cratoxylum cochinchinense which was harvested from Sook...
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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
... rhamnosus enzyme extracts 112 Fig 34 LC/MS-ESI spectra and proposed structure of artifact in negative ionization mode (m/z 253) produced after 60 h incubation with Lactobacillus rhamnosus enzyme ... encompassing UV detection method is difficult to achieve (Hubert, Berger et al 2005) and often two different gradients programs are utilized to achieve full soyasaponin detection An analytical ... their assistance and cooperation A special mention also goes to fellow postgraduate students: Yeo Chia Rou, Ruan Wei Mei, Wong Weng Wai and Wei Ying whom I have had the opportunity to work together...
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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
... homogenized with 225 ml of BPW, and incubated at 37oC for h and 24 h Chicken samples were obtained from two chicken meat processing plants Chicken carcasses were collected from the line at a processing ... Verocytotoxinproducing Escherichia coli O157 on Dutch dairy farms J Clin Microbiol 1998, 36, 3480-3487 22 Hitchins AD, Feng P, Watkins WD, Rippey SR, Chandler LA Escherichia coli and the Coliform ... Escherichia coli isolates by random amplification of polymorphic DNA Avian Dis 1998, 42, 431-451 29 Morgan D, Newman CP, Hutchinson DN, Walker AM, Rowe B, Majid F Verotoxin producing Escherichia...
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Báo cáo khoa học: " Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)" ppt
... Serosurvey of ex situ giant pandas (Ailuropoda melanoleuca) and red pandas (Ailurus fulgens) in China with implications for species conservation J Zoo Wildl Med 2007, 38, 559-566 Mainka SA, Qiu ... survey of giant pandas (Ailuropoda melanoleuca), and domestic dogs and cats in the Wolong Reserve, China J Wildl Dis 1994, 30, 86-89 Naylor MJ, Harrison GA, Monckton RP, McOrist S, Lehrbach PR, Deane...
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Báo cáo y học: "Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)" potx
... 2000, 9(7):589-597 Chao and Lin Chinese Medicine 2010, 5:17 http://www.cmjournal.org/content/5/1/17 86 Ko HC, Wei BL, Chiou WF: The effect of medicinal plants used in Chinese folk medicine on RANTES ... chronic liver injury leads to cirrhosis Anti-hepatotoxic enzymes include cytochrome P450 (CYP) super-family, or normalizing the levels of marker enzymes for the liver function test, such as glutamate ... medium, provided the original work is properly cited Chinese Medicine 2010, article BioMed Central Ltd article is and Lin; 5:17 Chao and Lin Chinese Medicine 2010, 5:17 http://www.cmjournal.org/content/5/1/17...
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Isolation and identification of components from ixeris sonchifolia hance as potential anti stroke agents
... 1.1.3.5 Traditional Chinese Medicine 23 1.2 Studies on Ixeris sonchifolia Hance 24 1.3 Rationale and aims 28 Chapter Preparation and bioactivity screening of fractions from Ixeris sonchifolia Hance ... Ixeris sonchifolia Hance Ixeris sonchifolia Hance (Fig.1.2.1), which belongs to the Compositae family, is a small and bitter perennial herb widely distributed in the northeastern part of China (Feng ... clinical trials conducted by the Xiyuan Hospital of the Chinese Academy of Traditional Chinese Medicine, and five other hospital sites in China In these studies, Diemailing Injection was shown...
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isolation, selection and identification bacillus subtilis from muddreg of beer
... INTRODUCTION The genus Bacillus have a great potential for extracellular enzymes Many of these enzymes are extracellular enzymes that hydrolyze large organic molecules Thus, genus Bacillus has ... Bacillus subtilis is able to synthesize and produce protease, amylase, and a number of axoenzyme (extracellular enzyme) The products nature of biotechnology, along with the ability to create and spore ... protease enzyme: Use micropipett to drop 5µl bacteria from LB liquid medium Drip 5µl bacteria on SMA medium repeat times and incubate in 40°C on 24 hours Bacteria strains have protease enzyme will...
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Purification and cloning of PCR products
... Restriction enzyme cloning It is common to incorporate restriction enzyme sites into the primers used to generate the PCR products (6) When the PCR product is digested with these restriction enzymes ... (see Protocol 6.1) This procedure results in the enzyme removing the unpaired terminal nucleotide, but the presence of the dNTPs means that if the enzyme removes the next nucleotide this is immediately ... introduce different restriction enzyme sites at the two ends of the PCR product to allow directional cloning into the doubly digested vector, with at least one of the enzymes generating a cohesive...
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Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
... chicken lysozyme chromatin domain contains a second, widely expressed gene Nucleic Acids Res 30, 463–467 Gilbert N, Boyle S, Fiegler H, Woodfine K, Carter NP & Bickmore WA (2004) Chromatin architecture ... repressed genes EMBO J 9, 3997–4006 97 Woodcock CL, Skoultchi AI & Fan Y (2006) Role of linker histone in chromatin structure and function: H1 stoichiometry and nucleosome repeat length Chromosome Res ... c-Myc regulates transcriptional pause release Cell 141, 432–445 153 Zippo A, Serafini R, Rocchigiani M, Pennacchini S, Krepelova A & Oliviero S (2009) Histone crosstalk between H3S10ph and H4K16ac...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... various hevamine variants on chitopentaose and colloidal chitin as substrate, respectively With chitopentaose all hevamine variants have their maximum activity at pH 2.0–3.0 Enzyme activity decreases ... selected mutants with chitopentaose as substrate at pH 4.2 Mutant Fig Enzyme activity of hevamine and hevamine mutants as a function of pH with 50 lM chitopentaose as substrate The enzyme concentration ... successfully soaked overnight in 1.5 M ammonium sulphate, pH 7.5, containing mM chitotetraose Soaking with chitopentaose and chitohexaose was not feasible because crystal contacts obstruct substrate...
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Báo cáo " Analysis and identification of multi-variate random pressure fields using covariance and spectral proper transformations " pdf
... modes studied by Jeong et al 2000 [5], by which POD modes observed differently in two cases Kikuchi et al 1997 [6] applied the POD to pressure field of tall buildings, then fluctuating pressure ... specified at nonuniform distributed pressure taps, J of Wind Eng.Ind Aerodyn 87 (1997) [6] H Kikuchi, Y Tamura, H Ueda, K Hibi, Dynamic wind pressure acting on a tall building model - Proper orthogonal ... orthogonal decomposition, J of Wind Eng Ind Aerodyn 69-71 (1997) 631 [7] Y Tamura, H Ueda, H Kikuchi, K Hibi, S Suganuma, B Bienkiewicz, Proper orthogonal decomposition study of approach wind-building...
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Báo cáo sinh học: "Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" pot
... glycoprotein Serpin (C16L) Capping enzyme subunit virion component virion component Early transcription factor IMV membrane protein DNA-dependent ATPase Capping enzyme subunit PolyA polymerase dsRNA ... electrophoresis followed by in-gel trypsin digests and peptide mass fingerprints for database searching [5] Using a similar gel-based strategy, three major early proteins associated with the virosomes ... displaying eleven C-terminal (ytype) fragment ions and one N-terminal (b-type) fragment ion Database searching of this tandem mass spectrum identified this peptide as ELESYSSSPLQEPIR, the partial sequence...
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báo cáo hóa học:"Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" docx
... glycoprotein Serpin (C16L) Capping enzyme subunit virion component virion component Early transcription factor IMV membrane protein DNA-dependent ATPase Capping enzyme subunit PolyA polymerase dsRNA ... electrophoresis followed by in-gel trypsin digests and peptide mass fingerprints for database searching [5] Using a similar gel-based strategy, three major early proteins associated with the virosomes ... displaying eleven C-terminal (ytype) fragment ions and one N-terminal (b-type) fragment ion Database searching of this tandem mass spectrum identified this peptide as ELESYSSSPLQEPIR, the partial sequence...
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báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... indicates that the Muc16 is either rapidly cleaved from the cell surface by a very active proteolytic enzyme or is an alternatively spliced form that is primarily secreted by the MOVCAR cells Our future ... mediated by the ovarian tumor marker CA125 Gynecol Oncol 2005:704-713 Connolly DC, et al.: Female mice chimeric for expression of the simian virus 40 TAg under control of the MISIIR promoter develop ... syngeneic mouse model for events related to ovarian cancer Carcinogenesis 2000, 21:585-591 Mabuchi S, et al.: RAD001 (Everolimus) delays tumor onset and progression in a transgenic mouse model...
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Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot
... fibrinolytic enzymes from these earthworms have been identified (Dong et al 2004) High-throughput production of these enzymes by recombinant DNA technology has been conducted in Escherichia coli ... post-translational modifications of the enzyme Application of the fibrinolytic enzymes has also been of great interest in Vietnam, focusing especially on the use of local enzyme sources The preliminary ... in Escherichia coli (Cho et al 2004 and Xu et al 2010) and Pichia pastoris (Ge et al 2005 and Sugimoto et al 2005) The recombinant enzymes expressed strong fibrinolytic activity both in vitro...
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Báo cáo y học: "Genome-wide prioritization of disease genes and identification of disease-disease associations from an integrated human functional linkage network" pps
... Ghosh D, Pandey A, Chinnaiyan AM: Probabilistic model of the human protein-protein interaction network Nat Biotechnol 2005, 23:951-959 Hamosh A, Scott AF, Amberger JS, Bocchini CA, McKusick VA: ... Psychiatry 2008, 16:343-354 Sedel F, Turpin JC, Baumann N: [Neurological presentations of lysosomal diseases in adult patients] Rev Neurol (Paris) 2007, 163:919-929 Hayasaka S: Lysosomal enzymes ... SM, Barber T, Ptak J, Silliman N, Szabo S, Dezso Z, Ustyanksky V, Nikolskaya T, Nikolsky Y, Karchin R, Wilson PA, Kaminker JS, Zhang Z, Croshaw R, Willis J, Dawson D, Shipitsin M, Willson JK,...
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báo cáo khoa học: " Screening and Identification of a Renal Carcinoma Specific Peptide from a Phage Display Peptide Library" pot
... carcinoma line A498 and a normal renal cell line HK-2 were obtained from Medical Academy of China (Beijing, PR China) Fetal calf serum (FCS) and Dulbecco’s modified eagle’s medium (DMEM) were purchased ... by National Natural Science Foundation of China (No.81172432), The Project Supported by Guangdong Natural Science Foundation of the People’s Republic of China (No.9151802904000002), Scientific ... of the People’s Republic of China (2008B030301082), Doctoral Initiating Project, and Natural Scientific Foundation of Guangdong Province of the People’s Republic of China (No.7301521) 17 References...
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Báo cáo y học: "Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus" pps
... 625014, China 2Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya’an 625014, China 3Epizootic Diseases Institute of Sichuan Agricultural University, Ya’an, Sichuan 625014, China ... western blots (Figure 2B, lane2) All the data indicated the tgK had good reactivity and specificity Enzyme linked immunosorbent assay (ELISA) was used to evaluate the affinity of antibody Microplates ... organs, provide a new diagnostic method to detect DEV, provide useful method and data for researching and clarifying the morbigenous mechanism of DEV Until now, there is no report about indirect...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25