titration curve of oxalic acid and sodium hydroxide
Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc
Ngày tải lên :
16/03/2014, 14:20
... Gymnemic acids inhibit rabbit glycer-
aldehyde-3-phosphate dehydrogenase and induce a
smearing of its electrophoretic band and dephosphoryla-
tion. FEBS Lett 579, 4333–4336.
Interaction of gymnemic acid ... average diameter of
unbound GA is > 30 nm and that of GA and c-CD complex is 2.2 nm,
similar to unbound c-CD, supporting the aggregate property of GA and
the inclusion complexation of GA by c-CD.
Abbreviations
CD, ... presence of
2.0 m
M a-CD, b-CD, and c-CD. (B) The concentration dependence
of c-CD on NMR spectra of 2.0 m
M GA. The ratios of c-CD to GA
are indicated.
Y. Izutani et al. Interaction of gymnemic acid...
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Báo cáo y học: Esterified Hyaluronic Acid and Autologous Bone in the Surgical Correction of the Infra-Bone Defects"
Ngày tải lên :
03/11/2012, 11:35
... Dental Sciences and Surgery, University of Bari, Bari, Italy.
Correspondence to: Prof. F.R. GRASSI, Professor and Dean, Department of Dental Sciences and Surgery - University of
Bari, P.zza ... Hyaluronic Acid and Autologous Bone in the Surgical Correction of
the Infra-Bone Defects
Andrea BALLINI, Stefania CANTORE, Saverio CAPODIFERRO and Felice Roberto GRASSI
Department of Dental ... grafting, 4 males and
5 females, all non smokers, with a mean age of 43,8
years for females, 40,0 years for males and 42 years for
all the group, in good health and with a mean depth
of the infra-bone...
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THÀNH PHẦN hợp CHẤT AXÍT béo và CAROTENOID của gấc fatty acid and carotenoid composition of gac (momordica cochinchinensisspreng) fruit
Ngày tải lên :
09/02/2014, 00:08
... findings of none
detectable. The authors also reported 101 ( 38 and 22.1 ( 15.2
µg β-carotene/g FW in extracted samples of aril and mesocarp,
respectively, and 16 and 9 µg/g of zeaxanthin and 35 and ... identification of major carotenoids, includes carotenoid
profiles, measuring both trans- and cis-isomers of lycopene and
β-carotene. We also provide a detailed fatty acid analysis of
gac seed and aril, ... linoleic and R-linoleic acids.
The aril also contains a significant, but varying, amount of
stearic acid and small amounts of cis-vaccenic, myristic, eicosa-
11-enoic, arachidic, and palmitoleic acids.
Our...
- 6
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- 4
Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
Ngày tải lên :
21/02/2014, 01:21
... producing
transformant and was used for the purification of GALA.
Growth of this transformant and subsequent purification of
the enzyme were performed as described in Materials and
methods. Purity of the preparation ... to facilitate purification
of GALA. The enzyme has a molecular mass of 48.5 kDa
and a pH optimum between 4 and 4.5. Incubations of ara-
binogalactans of potato, onion and soy with GALA resulted
initially ... Maldi-TOF MS analysis of the hydrolysis of arabinogalactans by GALA. Three different arabinogalactans were used: potato (A), onion (B),
and soy (C). Spectra were taken after 15 min (1) and 2 h (2) of...
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Báo cáo khoa học: Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) pptx
Ngày tải lên :
07/03/2014, 06:20
... protection of enzymes and membranes from the
detergent effects of excess fatty acids by sequestering
them, and (d) modulation of cell growth and differenti-
ation by transport of fatty acids to the nucleus ... uptake of fatty
acids across the plasma membrane and transport to
various subcellular organelles, (b) modulation of the
activity of enzymes involved in fatty acid metabolism,
(c) protection of enzymes ... consists of four exons of
112, 174, 89 and 141 bp, coding for 22, 59, 30 and 20
amino acids, respectively. The intron ⁄ exon structure of
the fabp6 gene from zebrafish is consistent with that of
all...
- 10
- 379
- 0
Báo cáo khoa học: Reconstruction ofde novopathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose inSaccharomyces cerevisiae pptx
Ngày tải lên :
07/03/2014, 12:20
... alternative route for
the production of UDP-glucuronic acid via
myoinositol has also been identified and
characterized.
Synthesis of UDP-glucuronic acid and UDP-xylose T. Oka and Y. Jigami
2646 FEBS Journal ... amounts of the nucleotide sugars, and also
discuss the implications of our results in yeast for the
study of metabolic regulation in plants.
Results
Cloning and expression of AtUGD1 and AtUXS3
genes ... inhibition constant (K
i
)of
AtUGD1. Inhibition by UDP-GlcA and UDP-Xyl was
measured with various concentrations of UDP-Glc and
saturating levels of NAD
+
. Double-reciprocal plots of
the data revealed...
- 13
- 541
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Báo cáo khóa học: Conformational changes of b-lactoglobulin in sodium bis(2-ethylhexyl) sulfosuccinate reverse micelles A fluorescence and CD study docx
Ngày tải lên :
07/03/2014, 15:20
... ratio of
native dimers to monomers, and the loss of H-bonding
involving the strands b
I
of bLG [47], could be promoted in
both situations. A CD spectrum assigned to distortions of
b-strands showed ... chirality of the environ-
ments of the side-chains of aromatic residues (Trp, Tyr and
Phe) and disulfide bonds [49]. The two deep peaks found at
286 and 293 nm arise from Trp residues. In the case of ... means of a curve obtained using appro-
priate fluorescence standards together with the standard
provided with the instrument. The quantum yields of
NATA and bLG were determined relative to that of...
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Báo cáo khóa học: Characterization of Mesorhizobium huakuii lipid A containing both D-galacturonic acid and phosphate residues ppt
Ngày tải lên :
07/03/2014, 15:20
... De-O-acylation of lipid A
fractionsledtodecayofspeciesZandYandresultedin
increase of signals corresponding to ions of species X
(data not shown). The total decrease of mass due to de-O-
acylation of phosphorylated ... protons and protons from a, b and c positions of 3-hydroxy fatty acids are marked with;
-CH ¼ CH-, -CH
3
,and- CH
2
-, a, b and c, respectively. CHCl
3
,DMSOandH
2
O represent signals from solvents and ... 2,3-diamino-2,3-dideoxyglucose
(DAG) and a complex set of fatty acids (both ester and
amide bound), as described previously [24], but also
galacturonic acid and phosphate residues. The presence of
GalA was unequivocally...
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Báo cáo Y học: Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells pdf
Ngày tải lên :
08/03/2014, 09:20
... Mendez, Cristina Paz and Ernesto J. Podesta
´
Department of Biochemistry, School of Medicine, University of Buenos Aires
Although the role of arachidonic acid (AA) in the regulation
of steroidogenesis ... of recombinant
CTE-I as antigen dissolved in 0.5 mL of distilled water and
mixed with equal volumes of Freund’s complete adjuvant,
andthreetimeswith500lg of antigen mixed with equal
volumes of ... reports have shown that arachidonic acid (AA)
and its metabolites play an essential role in the regulation of
steroidogenesis and both the expression and function of
StAR [17–26]. Adrenal cholesterol...
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- 470
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Báo cáo Y học: Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt
Ngày tải lên :
08/03/2014, 23:20
... independent regulation of
these two genes and of the accumulation of bryonolic acid
and phytosterols. It is noteworthy that the changes in their
mRNA levels correlated with the accumulation of the
respective ... cycle. These results suggest independent regulation
of these two genes and of the accumulation of bryonolic acid
and phytosterols.
Keywords: bryonolic acid biosynthesis; isomultiflorenol
synthase; Luffa ... growth, accumulation of bryonolic acid and total sterol, and
mRNA levels of isomultiflorenol synthase and cycloartenol
synthase in the cultured Luffa cells. Although...
- 7
- 491
- 1
Báo cáo khoa học: Plant oxylipins: role of jasmonic acid during programmed cell death, defence and leaf senescence doc
Ngày tải lên :
16/03/2014, 02:20
... to OPDA and JA synthesis [34]. By
contrast, fractions of the unsaturated membrane fatty
acid a-linolenic acid and a-linoleic acid are converted
randomly and nonenzymatically to a variety of prod-
ucts ... variety of abiotic and biotic cues.
To deter feeding insects, nematodes and fungal and bacterial pathogens,
plants have evolved a plethora of defence strategies. A central player in
many of these ... transition and the release
of cytochrome c in animal cells [112]. In A549 human
lung adenocancer cells, Me-JA operates through the
induction of proapoptotic genes of the BCl-2, Bax and
Bcl-X families and...
- 16
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- 0
Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identification of amino acid residues critical for eIF5A activity and hypusine modification doc
Ngày tải lên :
16/03/2014, 06:20
... to 30% of the initial rate of UBHY-R,
whereas the rates of synthesis of DNA and RNA were
70% and 60% of the initial values, respectively. These
data suggest that the primary effect of eIF5A ... sites of
p414GAL1 and the NdeI–BamHI sites of pET11a. The
entire ORF of the site-mutated and truncated eIF5A was
sequenced for confirmation of the intended mutation or
truncation.
Determination of ... mm
NAD, 25 lg of BSA, 3 lCi of [
3
H]spermidine, 0.1 lgof
DHS and 1.0 lg of DOHH and clarified BL21(DE3) lysates
containing heIF5As (2–5 lg). After incubation for 2 h at
37 °C, an aliquot of the reaction...
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Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen affinities of the a and b subunits potx
Ngày tải lên :
16/03/2014, 14:20
... on
oxygenation of liganded human hemoglobin
Oxygen affinities of the a and b subunits
Sergei V. Lepeshkevich and Boris M. Dzhagarov
Institute of Molecular and Atomic Physics, National Academy of Sciences of ... different effects of pH and NaCl on individual O
2
-binding properties
of a and b subunits within liganded tetramer and dimer of human hemo-
globin (HbA) were examined in a number of laser time-resolved ... in the absence of NaCl (a) and at a NaCl
concentration of 0.5
M (b), and 2.0 M (c). Insets shows residuals (a)
(b), and (c) from the double exponential fits of the curve (a) (b), and
(c), respectively....
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Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx
Ngày tải lên :
17/03/2014, 09:20
... an
extracellular loop of the Ntcp and could represent the
sodium ion sensor of sodium- coupled taurocholate trans-
port via Ntcp. In order to find out whether and to what
extent this amino acid affects sodium ... circulation of bile acids and therefore also
participate in the homoeostatis of cholesterol. Members of
SBATs are located either in apical membranes of ileum
enterocytes, kidney tubule cells and bile ... Site-directed mutagenesis of Ntcp (Eur. J. Biochem. 270) 1121
Transport of taurocholate by mutants of negatively charged amino
acids, cysteines, and threonines of the rat liver sodium- dependent
taurocholate...
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- 0
- titration between oxalic acid and sodium hydroxide
- titration oxalic acid and sodium hydroxide
- titration with oxalic acid and sodium hydroxide
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