... (generating 5.8S rRNA) or pBluescript (nonspecificRNA) in the presence of 10 mm NTPs. UV cross-linkingwas performed by placing RNA–protein complexes on iceand irradiation for 15 min at 260 nm using ... Nop53p binds 5.8S rRNA. UV cross-linking was performedafter incubation of 1 pmol of in vitro transcribed, uniformly labeled5.8S rRNA with increasing amounts of His–Nop53p or bovineserum albumin ... Analyzing protein–proteininteractions using two-hybrid system. Methods Enzymol254, 241–263.42 Zanchin NIT & Goldfarb DS (1999) Nip7p interactswith Nop8p, an essential nucleolar protein required...
... phenomena that are in- volved in determining answerhood.Our work suggests that considerable gains in performance can be obtained by incorporating TEduring both answer processing and passage re-trieval. ... in a text can beinferred from the information expressed in anothertext, can be performed in four ways. We can try to(1) derive linguistic information from the pair oftexts, and cast the inference ... corresponding entities, predicates, orphrases found in a pair of texts. In this section,we show that by using a machine learning-basedclassifier which combines lexico-semantic infor-mation...
... kiện và hoàn cảnh giảng dạy thực tế. Đối với học sinh THCS hiện nay, tiếng anh đã trở lên phổ biến và thầy cô giáo cũng đã cung cấp cho học sinh một vốn kiến thức mới, biết được các phương pháp ... xác và trôi chảy những điều cần thông tin. Tuỳ theo mục đích yêu cầu của bài học mà người dạy có thể lựa chọn một số kĩ thuật thích hợp để xây dựng các sinh hoạt học tập trong lớp và các bài ... Structured Interview là một ví dụ của sự điều chỉnh vừa nêu. Trong bài học này người học phỏng vấn lẫn nhau, sử dụng cấu trúc ngữ pháp đã học, nhưng họ trả lời với những thông tin có thật mà...
... and learning and (3) advantages of using computers in reading ESP teaching and learning.I.2.3.1.1.Model of teaching ESP reading with computersSome models of using computers in teaching FL can ... of computers for teaching and learning (in general) and teaching and learning FL (in particular). As far as this study is concerned, certain applications of computers in teaching and learning ... following main methods to do the research: Consulting books, journals and newspapers on applying IT in teaching and learning foreign languages or ESP; Searching information on the Internet...
... lựa chọn định dạng phong phú - Match Destination Formatting (chuyển định dạng dữ liệu vừa dán giống với định dạng của cả văn bản) và Keep Source Formatting (giữ nguyên định dạng của đoạn dữ liệu ... bản.Xoá thông tin cá nhânCác tệp tin văn bản của MS Word có chứa những thông tin ẩn có thể tiết lộ thông tin về bạn. Microsoft cũng đã cho ra công cụ xoá bỏ những thông tin ẩn này Remove ... MS Office chính là Format Painter – có hình dáng như chiếc chổi sơn trên thanh công cụ chuẩn (Standard toolbar). Nếu chưa có bạn có thể vào Tool | Commands, tìm đến mục Format và kéo biểu tượng...
... andpolice for everything from underage drinking to hit and runs. Be careful what you post in Facebook just like everywhere else online.Effective Internet Presence: Now requiredfor success in business ... the business requirements.”He has been on the Internet forever and using email since well before the term ‘email’ was ever coined.Ted has written several books on the Internet and Business, ... developing anEffective Internet Presence, including upcoming teleseminars and hopefully another ebook or two on the subject.More information about Ted is available via Google, or:Internet Business...
... Protein A Agarose ⁄ Salmon SpermDNA incubation for no more than 1 h. Beads were washedtwice for 15 min in lysis buffer containing 150 m m NaCl,followed by a 15-min wash in lysis buffer containing500 ... reduced in a strain lackingboth the Swi1 and Snf5 activator-binding domains.Recruitment of the SWI ⁄ SNF complex via activatorinteractions with these activator-binding domains isthus critical for ... promoters under inducing condi-tions (galactose, 30 min) in the wild-type strain (black bars, Wt),strain YPP310 (light grey bars, DDABDswi1 + snf5) lacking bothactivator-binding domains (SWI1D329-655,...
... of the gastrin–transferrin inter-action is still unknown. Knowledge of the regions oftransferrin requiredfor the binding of gastrin, and ofthe regions in gastrin requiredfor the interaction ... is requiredfor the Ggly–trans-ferrin interaction, to define the domains ⁄ residues ofGgly involved in the interaction (using Ggly mutants),and, finally, to determine the regions of transferrin required ... connection between gastrins and ironhomeostasis was first provided in a search for gastrin-binding proteins in porcine gastric mucosa [14]. Aninteraction between Gamide and transferrin was identi-fied...
... project into mini projects. In thekitchen, for example, mini projects might includecountertop, under the sink, cutlery drawers, pantry,refrigerator/freezer, recipes, and coupons. For 5 to 15 minutes ... organizing yourentire house. Start by gathering and getting things tothe rooms where they belong. In the second pass,you can begin to organize the contents of each room. can learn by reading ... following people:My colleagues in the National Association ofProfessional Organizers, for putting theprofessional in organizing.Everyone at Storey Publishing who has had a hand in creating...
... have found tiny amounts of things like heart medicine, hormones and pain killers in their drinking water. Here in Santa Clara County, scientists detected eight different drugs, including ibuprofen ... NEVINS: “Our study is mainly focusing on not the population-level impacts but how we can use the birds as samplers of the marine environment.” Nevins is using the fulmar stomachs as indicators ... Sunday morning, in late April at the Berkeley Marina dozens of anglers are casting their fishing rods over the pier, hoping for a bite. BERKELEY ANGLERS: Well we’re doing some halibut fishing… Yeah...
... obtained, as discussed in detail below, indicate a crucial role for sulfur–sulfurinteractions in defining the catalytically competentbinding mode of CoA in the active site.The pantetheine binding ... for increasing the affinity of binding, as well as the solub-ility of the substrate.Interestingly, the side chain of Ser247 points awayfrom SPP, as previously seen in unliganded thiolase; in the ... cor-responding distance for the CoA complex is 3.9 A˚(Table 2).The pantetheine moiety of SPP binds in the pant-etheine binding tunnel in a similar (but not identical)way to that seen for CoA...
... lgÆmL)1spectinomycin. For thylakoid isolationand physiological measurements, wild-type and transformedplants (originating from two independent transplastomiclines) were planted in compost and kept in ... wasexplained by a displacement of this domain. Thus,PSI-J does not appear to participate directly in bindingof Pc or Cyt c6, but plays a role in maintaining aprecise recognition site for the ... theluminal side of PSI involving PSI-F and PSI-N. Theabsence of PSI-J might affect the conformation ofPSI-F, which, in turn, changes the binding of PSI-N.PSI-F provides part of the Pc-binding...
... were grown in Eagle’s Minimum Essential medium(Nissui Pharmaceutical) containing 10% bovine serum in aCO2incubator at 37 °C.Screening for Hsp modulatorsA reporter plasmid containing the Hsp105 ... kept at 4 °Cfor15 min, and vortexed for 15 min at 4 °C. After centrifuga-tion at 50 000 g for 5 min, aliquots of the supernatant(15 lg protein) were incubated in 25 lL buffer containing10 mMTris/HCl ... for 15 min, Luc protein in thesupernatant (s) and pellet (p) fractions were detected by Western blotting using anti-Luc Ig [upper panels in (b)]. (C) The structure of p173ORplasmid containing...
... impli-cated in substrate binding, are shown in Fig. 3 to bewithin hydrogen bonding distance from the substrate.As energy minimization only allows for limited con-formational sampling, we also ... monitored by the increase in emission at 400 nm over time using kexcitation= 325 nm.The mcaGnRH1–9substrate was monitored by HPLC(Agilent 1100) using the increase in peak area for theemission ... regions lining the substrate-binding site allow the enzyme toconform to substrates of varying structure. This study describes mutantforms of thimet oligopeptidase in which Gly or Tyr residues in...
... plated in media containing Geneticin to select for cellcontaining vector DNA. After three weeks, the number of cells were determined by fixing cells and staining with dye and quantified in (H). ... media containing Geneticin toselect for cell containing vector DNA (as in Fig. 5A,G). After three weeks, the number of cells was determined by fixing cells and stainingwith dye. Individual colonies ... structure to SEP53 containing anN-terminal EF-hand calcium-binding domain andmultiple C-terminal amino acid repeat sequences. Using a protein BLAST search, several proteins on the1q21 locus...