thực trạng tình hình tài chính công ty 5
Retrovirology Research BioMed Central Open Access A novel HIV-1 restriction factor that is potx
... (0.04%) (0.13%) - C (0. 05% ) (0.04%) (0.09%) (0.02%) (0.03%) N= 55 00 15 (0.02%) G (0.02%) (0.27%) C (0.02%) (0.04%) 0 (0.02%) N= 55 00 Figure Characterization of inhibitory activity of CEM.NKR Characterization ... and A3F Q275E mutants retain anti-HIV-1 activity The cDNAs of A3G R14Q and A3F Q275E were amplified from CEM.NKR by RT-PCR and cloned into pcDNA3.1/V5-His-TOPO vector (Invitrogen) Wild-type or vifdefective ... infectivity significantly more than HIV-1 virus infectivity (Fig 1C) For example, A3G, A3G R14Q, A3F, or A3F Q275E reduced HIV-1 infectivity by 12, 8, 60, or 16-fold and HIV-1ΔVif infectivity by...
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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
... Total no Age < 50 years ‡ 50 years Tumor size £ cm > cm Lymph node metatasis No Yes Histology Invasive ductal carcinoma Medullary carcinoma 10 (43 .5) 13 (56 .5) (30.0) (70.0) (46.2) (53 .8) Anchorage-independent ... Colonies were stained with 1. 25 mgÆmL)1 nitroblue tetrazolium for 16 h before imaging (50 .0) (50 .0) (53 .8) (46.2) Statistical analysis (40.0) (60.0) (61 .5) (38 .5) 10 (100) (0) 12 (92.3) (7.7) ... microRNA genomics Nucleic Acids Res 36, D 154 –D 158 He L & Hannon GJ (2004) MicroRNAs: small RNAs with a big role in gene regulation Nat Rev Genet 5, 52 2 53 1 Evan GI & Vousden KH (2001) Proliferation,...
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báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt
... Heterozygosity pM (matching probability) THO-1 D5S592 D10S526 vWA31 D22S417 FES/FPS 154 –178 166–206 182–266 127–167 172–213 206–234 0.77 0.83 0.84 0.81 0. 85 0.67 0.086; out of 12 0. 051 ; out of ... extremity Page of (page number not for citation purposes) Journal of Translational Medicine 2008, 6: 75 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 amputation complicating type diabetes: ... 0. 051 ; out of 20 0.017; out of 59 0.062; out of 16 0.039; out of 25 0.1 65; out of Also available online at http://ccr.coriell.org/Sections/Support/Global/QCgenotype.aspx?PgId=412 Page of (page...
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Báo cáo sinh học: "Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells" pptx
... 70. 95 21.09 51 .47 31.28 41 .55 34 .56 5. 30 0.90 19.96 0.43 12.83 54 .06 25. 08 9. 95 76.79 91.09 7.09 0.04 1.88 93 .59 PI 0.67 Apoptosis(%) 5. 55 2.99 4.49 82.29 0. 15 12.26 Kasumi-1 91.83 1.01 U937 ... of 15 Curcumin( M) 40 60 80 KG1a Kasumi-1 U937 B Curcumin( M) 40 60 0.02 1.81 80 2.66 0 .56 3.83 38.27 76.2 19.80 70.89 25. 78 57 .34 0.03 7.93 0.03 17.22 0.00 23.89 70. 95 21.09 51 .47 31.28 41 .55 ... M) 40 60 93. 25 6.47 97.31 80 37. 65 19.14 23.86 62.33 80.82 92.88 72.29 49.91 38.27 KG1a Red 76.13 Kasumi-1 27.68 50 . 05 84.76 20. 25 7. 25 0.36 15. 22 79.73 92.74 99.63 7.08 U937 2 .58 Red/green ratio...
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báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... 618F, 653 F and VK-8 to these three cell lines is comparable SKOV-3 OVCAR-3 Absorbance at 280 nm Pools 1-2 5. 0 4.0 3.0 2.0 Pool 1.0 0.0 Mean Fluorescence Intensity 50 10 15 20 25 30 35 40 45 50 55 ... Fluorescence Intensity 60 Intracellular Mean Fluorescence Intensity 65 55 Extracellular Intracellular 50 45 40 35 30 25 20 15 10 20 MOVCAR-1 MOVCAR-2 MOVCAR-9 MOVCAR-10 MOVCAR-1 MOVCAR-2 MOVCAR-9 ... http://www.ovarianresearch.com/content/2/1/8 a Mean Fluorescence Intensity b 25 a 653 F 20 15 10 CAOV-3 Figure Specificity of 618F for human MUC16 Specificity of 618F for human MUC16 (A) Purity of human MUC16 isolated from spent...
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Báo cáo y học: " Inhibition of constitutively active Jak-Stat pathway suppresses cell growth of human T-cell leukemia virus type 1-infected T-cell lines and primary adult T-cell leukemia cells" potx
... activity of NFATc1 is enhanced by the Pim-1 kinase J Immunol 2002, 168(4): 152 4- 152 7 Galaktionov K, Chen X, Beach D: Cdc 25 cell-cycle phosphatase as a target of c-myc Nature 1996, 382( 659 1) :51 1 -51 7 ... HUT-102: from 51 % to 83%; ED4 051 5(-): from 35% to 44%] and decreased percentage of cells in S phase [MT-2: from 36% to 18%; HUT-102: from 36% to 8%; ED-4 051 5(-): from 51 % to 43%], indicating G1 ... phosphorylation of Stat5 and Jak3, and DNA-binding activity of Stat5 of Constitutive activation of Stat3- and Stat5-DNA binding activity in HTLV-1-infected T-cell lines Electrophoretic mobility shift assay...
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Evaluation of thymoquinone for cytotoxic activity against human breast cancer cell lines and tumor xenograft in nude mice
... apoptosis 85 v 3 .5. 5 3 .5. 6 Effect of p38 siRNA gene silencing on TQ-induced apoptosis 89 3 .5. 7 The relationship between ROS and p38 in the mechanism of action of TQ 91 3 .5. 8 3.6 Effect ... SB20 358 0 on TQ-induced p38 activation 83 Figure 3 .5. 3 Effect of SB20 358 0 on TQ’s growth inhibition effect 84 Figure 3 .5. 4 The role of p38 activation on TQ-induced apoptosis .86 Figure 3 .5. 5 Effect ... 50 3.1 .5 Effect of TQ on apoptotic pathway 52 iv 3.2 Studies on the anti-metastatic effect of TQ in breast cancer cells 54 3.2.1 3.2.2 3.3 Effect of TQ on cell migration 54 ...
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Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
... Tris/HCl pH 7 .5/ 0. 15 M NaCl and incubated overnight at °C with anti-DIG Ig (1 : 50 00 dilution, Roche) in 0.1 M Tris/HCl pH 7 .5/ 0. 15 M NaCl Slides were rinsed in 0.1 M Tris/HCl pH 7 .5/ 0. 15 M NaCl, ... 9 .5/ 0.1 M NaCl /50 mM MgCl2 and incubated with 262 .5 lgÆmL)1 Nitro Blue tetrazolium (Roche) and 1 75 lgÆmL)1 5- bromo-4-chloroindol-2-yl phosphate 10 (Roche) in 0.1 M Tris/HCl pH 9 .5/ 0.1 M NaCl /50 ... in the cell cycle Cell 79, 54 7 55 0 Blow, J.J & Laskey, R.A (1988) A role for the nuclear envelope in controlling DNA replication within the cell cycle Nature 332, 54 6– 54 8 Blow, J.J & Hodgson,...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
... q+ value: *P < 0. 05; ***P < 0.001 Conditions Standard growth Bone Muscle Lung a q+ q0 q+ q0 q+ q0 Stressa 186 142 208 143 2 35 25 12 75 250 0 1 055 996 1693 976 ± ± ± ± ± ± 33 (4) 75 (6) (3) (3)*** ... Citrate synthase Methods Enzymol 13, 3 5 Anderson, M.E (19 85) Determination of glutathione and glutathione disulfide in biological samples Methods Enzymol 113, 54 8 55 5 Floreani, M., Petrone, M., Debetto, ... 276, 56 9 57 5 51 Allalunis-Turner, M.J., Lee, F.Y & Siemann, D.W (1988) Comparison of glutathione levels in rodent and human tumor cells grown in vitro and in vivo Cancer Res 48, 3 657 –3660 52 Dickinson,...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
... epitope FRET efficiency E (% ± SEM) Kit225K6 Kit225K6 Kit225K6 Kit225K6 Kit225K6 Kit225K6 Kit225K6 Kit225K6 CD48 CD48 CD 25 CD 25 CD 25 CD 25 CD48 CD48 CD 25 CD 25 HLA-DR HLA-DR CD71 CD71 CD71 CD71 12.6 ... 483± 15 nm excitation filter, a 50 0-nm dichroic mirror and a 51 8±28 nm emission filter, while the red fluorescence was detected by a 54 8±10 nm excitation filter, a 57 8-nm dichroic mirror and a 58 4-nm ... investigated This was the tyrosine phosphorylation of STAT3/STAT5, monitored through binding of anti-(phospho-tyr-STAT3/STAT5) Ig As Fig 5B shows, stimulation of the Kit225K6 T cells with 1000 UÆmL)1...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... 6.8 ± 2.2 lm [ 95% confidence interval (CI): 2.6–11.4] and 6 .5 ± 2 .5 lm ( 95% CI: 1 .5 11 .5) , respectively The IC50 values of selenoamide A and selenoamide B were 11.3 ± 4.8 lm ( 95% CI: 2.0–20.6) ... Immunol 169, 52 52 52 59 51 Kimura H & Nakano M (1988) Highly sensitive and reliable chemiluminescence method for the assay of superoxide dismutase in human erythrocytes FEBS Lett 239, 347– 350 52 Nakano ... 54 Root RK, Metcalf J, Oshino N & Chance B (19 75) H2O2 release from human granulocytes during phagocytosis I Documentation, quantitation, and some regulating factors J Clin Invest 55 , 9 45 955 ...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... Biophys Acta 1610, 141– 153 Gossen M & Bujard H (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters Proc Natl Acad Sci USA 89, 55 47 55 51 Fast set-up of inducible ... cells, the median fluorescence intensity was 1 95 in the off-state and 1860 in 786 Doxycycline – Relative background transcription (%) 0.014 0.014 0.0011 0.0 052 0.0 45 0.0017 0.068 0.0077 0.031 0.012 ... Cell Biol 5, 253 3– 254 2 Young JM, Cheadle C, Foulke JS Jr, Drohan WN & Sarver N (1988) Utilization of an Epstein–Barr virus replicon as a eukaryotic expression vector Gene 62, 171–1 85 Sclimenti...
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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
... Combination 20 Percent of control Percent of control 120 200 150 PLX4032 Metformin 100 Combination 50 0 0 0. 05 0.1 0 .5 2 .5 5 10 10 20 20 0. 05 0 .5 2 .5 10 20 40 0.1 10 20 40 PLX [μM] + Metformin [mM] PLX ... 60 40 Metformin Combination 20 100 80 PLX4032 60 Metformin 40 20 Combination 0 0. 05 0 .5 2 .5 10 20 0. 05 0 .5 2 .5 10 20 0.1 10 20 40 0.1 10 20 40 PLX [μM] + Metformin [mM] PLX [μM] + Metformin [mM] ... if IC50 < μM; intermediate sensitive with IC50 1-10 μM; resistant if IC50 > 10 μM Sensitivity to single agent metformin: highly sensitive if IC50 < 10 mM; intermediate sensitive with IC50 10-20...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... 36 .51 5. 17 10.69 * HOS 42 .58 4.86 ACHN 9. 85 0.20 * SW 1 353 42.22 4.42 SK-NEP-1 0.00 1.82 HT 1080 19.37 5. 92 SA-4 12 .53 1. 05 ** SW 1961 64 .55 3.04 HS 919 3.01 5. 31 KATO-III 7. 65 2.16 SW 80 -5. 00 ... -56 . 35 1. 45 ** SW 732 ** DLD-1 69.19 65. 59 1.29 3.19 HS 913T Lung (4/11) Non-small cell (2/7) Gastric (1/2) Colorectal (5/ 6) ** SW 608 53 .11 5. 15 * A427 27.71 6.87 ** SW 707 52 .38 0.64 * SW 157 3 ... only -2. 35 0.86 Lung Fibroblasts -1.03 Ditt Fibroblasts -0.13 ** GM-CSF + IL-6 56 .30 SEM ** HeLa 68. 35 5.36 ** ME-180 75. 24 3.83 0.96 ** SIHA 54 .49 8.66 2.91 ** RL 95- 2 52 .11 3.84 5. 01 SW 756 -83.60...
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Báo cáo sinh học: " High Chromosome Number in hematological cancer cell lines is a Negative Predictor of Response to the inhibition of Aurora B and C by GSK1070916" potx
... modality in NHL B-Cell Lymphoma subtypes NHL Subtype >2n >3n Total Cases Diffuse Large 27 .5% 13.7% 12 25 Follicular 18.3% 8.0% 1330 Mantle Burkitt 9.7% 6.4% 7.7% 2.0% 402 659 MALT 5. 9% 3 .5% 340 ... high modality in major hematological patient populations Tumor Type >2n >3n Total Cases AML 4.6% 1 .5% 14,611 B-ALL 25. 0% 2.0% 3,769 NHL - B-Cell NHL - T-Cell 14.8% 7.2% 8.2% 5. 1% 3 ,54 2 1,497 Hodgkins ... intensities were normalized to a value of 150 using the mas5 algorithm in the Affymetrix Microarray Analysis Suite 5. 0 For subsequent analysis, the average probe intensity was used for triplicates Values...
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Báo cáo hóa học: " Quantitative profiling of housekeeping and Epstein-Barr virus gene transcription in Burkitt lymphoma cell lines using an oligonucleotide microarray" docx
... 0. 25 0.01 0. 05 0.00 0.18 0. 75 1.04 1. 35 1.18 1. 35 1. 35 1. 35 1.29 0.10 0. 15 0.04 0.11 0.04 0.03 0. 05 0.02 0.13 0.14 0.03 0.09 0.03 0.02 0.03 0.01 0. 45 1.22 1 .57 0.90 2.44 1.98 2.22 0.71 0 .59 0.63 ... Hs.194148 70 53 9 73.6 52 .2 CS MHCI.70 mer Hs.379218 70 2290 600 92.7 55 .7 PE HMBS.70 mer Hs.82609 70 153 6 1300 97.7 57 .0 PE HMBS.2 Hs.82609 70 164 82.4 52 .2 CS EF1.70 mer Hs.1811 65 71 50 0 91.8 50 .7 PE ... 0. 05 0. 05 1. 65 0.01 0.01 1.44 0.01 0.01 1.33 0. 05 0.04 1.37 0.23 0.17 1.32 0. 45 1.73 1.03 0.02 0. 05 0. 05 0.02 0.02 0.10 0.03 0.13 2. 05 0.11 0.48 2.42 0.42 0.20 0.03 0.32 0.13 0.27 0.72 0.30 1 .51 ...
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