three dimensional culture systems to induce chondrogenesis of adipose derived stem cells
Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
Ngày tải lên :
09/08/2014, 10:23
... bone marrow into the bloodstream of patients with a variety of malignancies The stem cells are then harvested from the blood as a source of stem cells to be returned to patients following chemotherapy ... stromal cell -derived factor expression by TNF injection stromal cellsstromal cell -derived factor expression marrow decreases by bone marrow stromal cells Expression of stromal cell -derived factor (SDF-1) ... chemokine system controlling mobilization of hematopoietic stem cells and progenitors – mediates TNFinduced OCP mobilization, we first verified that OCPs express functional CXCR4 and migrate toward...
- 10
- 386
- 0
Báo cáo y học: "Pro-inflammatory properties of stromal cell-derived factor-1 (CXCL12) in collagen-induced arthritis" pptx
Ngày tải lên :
09/08/2014, 07:20
... leukocytes to produce this cytokine To help distinguish between these two possibilities, we tested the ability of CXCL12 to induce the production of IL-6 in splenocyte cultures Splenocytes of CII/CFA-immunized ... reduced the number of harvested cells to that of the negative control The number of cells in the air pouch of AMD3100-treated mice after challenge with 0.9% NaCl was similar to that in the negative ... into subcutaneous air pouches in vivo In addition, we found CXCL12 to be able to enhance receptor activator of NF-κB ligand (RANKL)-induced osteoclast differentiation from splenocytes and to...
- 13
- 356
- 0
Báo cáo khoa học: Syndecan-4 is a signaling molecule for stromal cell-derived factor-1 (SDF-1)/ CXCL12 pptx
Ngày tải lên :
16/03/2014, 18:20
... phosphorylation of CXCR4, tyrosine phosphorylation of SD-4 occurs in response to SDF-1 stimulation of HeLa cells This tyrosine phosphorylation depends on the time of incubation of the cells with the ... hetero-oligomerization of CXCR4 induced by SDF-1 on HeLa cells is prevented by heparitinases I and III pretreatment of these cells Heparitinases I and III pretreatment of the HeLa cells also significantly ... tyrosine phosphorylation of SD-4 induced by SDF-1 on HeLa cells depends on HS, we treated these cells with mixtures of heparitinase I and III prior to their stimulation by SDF-1 To preserve cell viability,...
- 15
- 423
- 0
Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot
Ngày tải lên :
19/06/2014, 08:20
... ITAMs derived from the CD3 ζ (zeta) chain component of the T cell receptor Addition of the part of the small intracellular domain of the co-stimulatory receptor molecule CD28 has been shown to improve ... inserted into lentivirus/VSV pseudotypic vectors which were subsequently used for transduction of PBLs A weakness of many prior attempts has been failure to take into account the need for T cells to ... tumor-infiltrating T -cells [25-29], inability of a tumor antigen to induce high avidity T -cells [30,31] and possibly most significant and general of all, tumor cells commonly down-regulate expression of either...
- 10
- 435
- 0
báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" docx
Ngày tải lên :
20/06/2014, 04:20
... ITAMs derived from the CD3 ζ (zeta) chain component of the T cell receptor Addition of the part of the small intracellular domain of the co-stimulatory receptor molecule CD28 has been shown to improve ... inserted into lentivirus/VSV pseudotypic vectors which were subsequently used for transduction of PBLs A weakness of many prior attempts has been failure to take into account the need for T cells to ... tumor-infiltrating T -cells [25-29], inability of a tumor antigen to induce high avidity T -cells [30,31] and possibly most significant and general of all, tumor cells commonly down-regulate expression of either...
- 10
- 411
- 0
Báo cáo y học: "Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection" pptx
Ngày tải lên :
13/08/2014, 01:20
... R-computing software was used to plot the graphs Abbreviations MSCs: mesenchymal stem cells; ASCs: adipose tissue derived mesenchymal stem cells; HD: hematopoietic differentiated cells; AIDS: ... analysis of the differentiation potential of human adipose- derived adult stem cells J Cell Physiol 2006, 206:229-237 Fukuda K: Reprogramming of bone marrow mesenchymal stem cells into cardiomyocytes ... HPCs: hematopoietic progenitor cells; LTBMC: long-term bone marrow cultures; HD-5: differentiate into HD cells for days; HD-8: differentiate into HD cells for days; MOI: multiplicity of infection;...
- 12
- 364
- 0
Cloning of medaka glial cell derived neurotrophic factor (GDNF) and its receptor GFR alpha 1
Ngày tải lên :
03/10/2015, 20:57
... Spermatogonial Stem Cells (SSCs) Differentiation of germ cells in the testis originates from a constantly renewed small pool of stem cells, termed SSCs located at the basement membrane of the ... INTRODUCTION 1.1 Spermatogonial Stem Cells (SSCs) 1.2 Glial Cell Derived Neurotrophic Factor (GDNF) 1.3 GFL-induced activation of the protein tyrosine kinase, RET requires GDNF Family Receptor-α (GFRα) ... spermatogonia and numbered no more than 0.03% of the total number of germ cells (Tegelenbosch and de Rooij 1993) Depending on the signals produced by the surrounding Sertoli cells, As spermatogonia...
- 48
- 214
- 0
báo cáo hóa học:" Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy – a case report" doc
Ngày tải lên :
18/06/2014, 15:20
... L1 to L6 and L7 to L9 against K562 cells (A) and autologous In vitro (B) In vitro cytolytic activity of patient -derived PBMNC derived from L1 to L6 and L7 to L9 against K562 cells (A) and autologous ... A B Anastomotic relapse C Figure A- Photomicrograph view of the patient -derived cell line of the anastomotic relapse A- Photomicrograph view of the patient -derived cell line of the anastomotic ... (lymphocytes) CD3 (T cells) CD4 (helper T cells) CD8 (cytotoxic T cells) CD56 (NK cells) CD1a (APCs) CD25 (IL-2 receptor) Perforin (apoptosis inducer) Granzyme B (apoptosis inducer) ++++* ++ +++...
- 18
- 541
- 0
Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
Ngày tải lên :
18/06/2014, 19:20
... function of tumor-educated myeloid cells was measured by their ability to inhibit the proliferation of autologous T cells in the following Suppression Assay: T cells isolated from 30 mL of PBMC ... MDSC induction Myeloid cells isolated from cytokine-treated cultures showed significant suppression of autologous T cell proliferation (p
- 20
- 575
- 0
Báo cáo sinh học: "Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model" potx
Ngày tải lên :
18/06/2014, 22:20
... although bone marrowderived MSC is the major source of stem cells in these studies [22,24-27], the therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury of the lung has ... Use of Laboratory Animals (NIH publication No Page of 13 85-23, National Academy Press, Washington, DC, USA, revised 1996) Animal Grouping and Isolation of Adipose- Derived Mesenchymal Stem Cells ... belomycin-induced lung injury through suppressing the generation of pro-inflammatory cytokines and inflammatory reaction [19-23,25,26] One Page of 13 Figure Adipose- Derived Mesenchymal Stem Cell...
- 13
- 280
- 0
Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
Ngày tải lên :
07/08/2014, 23:22
... factors-mediated immunomodulatory effects of canine adipose tissue -derived mesenchymal stem cells Stem Cells Dev 2008, 17, 681-693 Kang SK, Lee DH, Bae YC, Kim HK, Baik SY, Jung JS Improvement of ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells ... Halvorsen Y, Storms RW, Goh B, Kilroy G, Wu X, Gimble JM Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006,...
- 12
- 309
- 0
Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx
Ngày tải lên :
10/08/2014, 23:21
... mechanism of regeneration is not yet clear It could be through direct differentiation of stem cells that were introduced to the diseased joints Alternatively, it could be due to the tropic effects of ... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect of full-thickness...
- 8
- 293
- 0
Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf
Ngày tải lên :
13/08/2014, 13:20
... reaction We co-cultured serial dilutions of DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for ... Regulation of T cell cytokine production by dendritic cells generated in vitro from hematopoietic progenitor cells Cell Immunol 2001, 208:115-124 Demangel C, Bertolino P, Britton WJ: Autocrine IL-10 ... differentiation of naive T cells [8-10] Efficient gene transfer into DCs without cytotoxicity has always been difficult [11,12] LVs transduce DCs at high efficiencies with little to no cytotoxicity,...
- 12
- 230
- 0
Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
Ngày tải lên :
14/08/2014, 08:20
... Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of ... Detection of smooth muscle cells after differentiation of the BMP2+ cells Figure Detection of smooth muscle cells after differentiation of the BMP2+ cells (a) Expression of SMA in the BMP2+ cells ... locations within the embryo to give rise to most structures of the peripheral nervous system, such as Schwann and glia cells of the autonomic and enteric nervous systems, endocrine cells, such as the...
- 28
- 288
- 0
Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc
Ngày tải lên :
14/08/2014, 18:20
... and interactions Figure Cytokine expression profiles of cells Cytokine expression profiles of cells Cytokine production by mDCs and the DC models in response to LPS Cytokine concentrations were ... research Cytokine expression profile Peripheral blood mDCs were found to express significantly higher levels of key inflammatory (IL-1β, IL-6 and IL-8) and regulatory (IL-10) cytokines, compared to moDCs ... regulation of MHC class II trafficking to DCs [8] Therefore, KG1 cells are considered to be a good model system to study human DC biology Despite the extensive use of both moDC and KG1 cells as...
- 13
- 417
- 0
Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
Ngày tải lên :
09/09/2015, 18:56
... generation of large quantities of stem cells difficult One of the most-well studied is the adult hematopoietic stem cells, which give rise to all the blood cells Hematopoietic stem cells are in ... human induced pluripotent stem cell derived- neural stem cell expressing CodA human induced pluripotent stem cell derived- neural stem cell expressing Fcy human induced pluripotent stem cell derived- neural ... to cancer gene therapy 21 1.3.5 Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells...
- 134
- 439
- 0
Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
Ngày tải lên :
26/11/2015, 09:54
... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... performed in MJD-lt-NES cells gained from patient -derived induced pluripotent stem (iPS) cells 3.2 Differentiation of lt-NES cells into neuronal and astrocytic cultures Lt-NES cells represent a standardized ... In vitro differentiation of hPS cells into lt-NES cells 43 3.2 Differentiation of lt-NES cells into neuronal and astrocytic cultures 43 3.3 Immunocytochemical analysis ...
- 135
- 365
- 0