the vasopressin receptor of corticotroph pituitary cells

Báo cáo Y học: Repression of FasL expression by retinoic acid involves a novel mechanism of inhibition of transactivation function of the nuclear factors of activated T-cells pptx

Ngày tải lên : 24/03/2014, 03:21

... elements in the FasL promoter (A) Schematic representation of the deletions in the 5¢ terminus of the FasL promoter that were cloned upstream of a luciferase reporter gene The 3¢ end of the FasL ... greater in the presence of retinoid receptors (Fig 4) Therefore, further investigations into each of these potential mechanisms are warranted, in order to further understand the retinoid receptor- induced ... and receptor subtype-selective analogues, may sub serve the role of such agents Recently, the physiological importance of NFAT in cells other than those of the immune system has been uncovered The...

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báo cáo hóa học:" Three-dimensional growth as multicellular spheroid activates the proangiogenic phenotype of colorectal carcinoma cells via LFA-1-dependent VEGF: implications on hepatic micrometastasis" docx

Ngày tải lên : 18/06/2014, 15:20

... on the 18th hour of incubation of monolayer- and 3D-spheroid-cultured CT26 cells For both culture conditions, the concentration of VEGF was expressed as a function of the total number Page of ... over-expression of this integrin enhanced the adhesion of CT26 to ICAM-1-expressing angiogenic hepatic myofibroblasts and endothelial cells; and endowed CT26 cells with the capability to further increase ... 3D-spheroid-cultured CT26 cells compared to monolayer-cultured cells; and the addition of μg/ml anti-murine LFA-1 antibody completely abrogated the adhesion of 3D-spheroid-cultured CT26 cells, but not of monolayer-cultured...

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Báo cáo hóa học: " Effects of pentacene-doped PEDOT:PSS as a holeconducting layer on the performance characteristics of polymer photovoltaic cells" pot

Ngày tải lên : 20/06/2014, 23:20

... pentacene The work function of PEDOT:PSS has been limited by charge collection because the work function of PEDOT:PSS film is higher than that of the HOMO level of pentacene The bandgap of the pentacene-doped ... mobility of the buffer layer were improved As the amount of pentacene was increased, the work function decreased The bandgap of the pentacene-doped PEDOT:PSS film has been approached to the ITO ... conceived the study, carried out the fabrication of photovoltaic cells, and drafted the manuscript JL and SO estimated the photovoltaic cells and helped analyze the data YC helped to develop the idea,...

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phosphatidylserine exposure in red blood cells: a suggestion for the active role of red blood cells in blood clot formation

Ngày tải lên : 15/11/2015, 12:54

... cells while the PS level in the outer membrane leaflet of the remnant cells remains low [19] A detailed analysis of sickle cells showed that PS exposure is limited to a subpopulation of the cells, ... localized in the endomembrane systems of eukaryotic cells and in the plasma membranes of some specialized cells They couple ATP hydrolysis with the transport of protons across membranes They also ... unfractionated cells However, the dense cells were also smaller, and the concentration of ATP in these cells was the same as in controls Therefore, it seems unlikely that loss of cellular ATP...

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Analysis of the mechanical reaction of human endothelial cells to a single constant or transient uniaxial strain

Ngày tải lên : 19/11/2015, 15:46

... using MCode commands [39] The speed of the stretcher motor was set to mm/s The speed of the XYstage was calculated from the speed of the stretcher, the stretch amplitude, and the jog distance in x-axis, ... 2.14 The scanning path of the substrate The inner part of the substrate is 10x10 mm2 However, because of the objective geometry and the clamping, the area available for imaging is 7x8 mm2 The ... neighbouring cells The mechanical reaction of endothelial cells to uniaxial strains is of primary interest in this work The cells are able to alter their internal stresses and forces they apply...

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Báo cáo khoa học: Involvement of the V2 receptor in vasopressin-stimulated translocation of placental leucine aminopeptidase/oxytocinase in renal cells pdf

Ngày tải lên : 08/03/2014, 02:20

... speciﬁc V2 receptor agonist, DDAVP On the other hand, the potent V2 receptor antagonist, AEAVP, completely suppressed the vasopressininduced translocation of the enzyme, while the V1a receptor ... surface receptors (V1a, V1b and V2) [22] To elucidate the role of vasopressin receptors, we examined the effects of vasopressin agonists and antagonists on the hormone-induced translocation of P-LAP ... indicate that the binding of the hormone to V2 receptors on the basolateral membrane of principal cells stimulates cAMP synthesis, which leads to the translocation of AQP-2 to the apical membrane [31]...

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Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx

Ngày tải lên : 07/03/2014, 21:20

... in which the N-terminus of the OTR is replaced with the corresponding sequence of the V1aR [10] The role of the N-terminus has been addressed recently by alanine-scanning mutagenesis of the N-terminus ... to maintaining the conformational switch of the V1aR in the off-state The unique ability of arginyl to fulﬁl this constraining function in the N-terminus of the V1aR is similar to the unique constraining ... nevertheless a signiﬁcant aspect of the Arg at this locus in the wildtype V1aR because reversal of the charge in [R46E]V1aR and [R46D]V1aR resulted in the least responsive receptors of the 20...

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Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx

Ngày tải lên : 16/03/2014, 14:20

... region The association of WASP and the Arp2 ⁄ complex activates the actin nucleation activity of the Arp2 ⁄ complex [23] To investigate further the function of the WASPEVH1 domain in the TCR ... consisting of VH and VL regions with ⁄ without the VH leader sequence at the N-terminal and with ⁄ without the CL(j) region behind the VL region None of the constructs contained the KDEL sequence at the ... respectively The appropriate cDNA fragments of the VH and VL regions were then generated by RT-PCR A comparison of the VH and VL amino-acid sequences of clones 18 and 21 is shown in Fig 1B All of the...

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Báo cáo khoa học: Cell type-speciﬁc transgene expression of the prion protein in Xenopus intermediate pituitary cells ppt

Ngày tải lên : 16/03/2014, 14:20

... approach with the intermediate pituitary melanotrope cells of the South-African clawtoed frog Xenopus laevis Depending on the colour of the background of the animal (black or white), these cells are ... suggesting that the level of GFP–PrPC transgene expression was dependent on the colour of the background of the animal The fusion protein was found only in the NIL and not in the AL of black- and ... expression of the GFP– PrPC fusion protein was restricted to the intermediate pituitary melanotrope cells and its level depended on the background colour of the animal Biosynthesis of newly synthesized...

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Báo cáo Y học: Loss-of-function variants of the human melanocortin-1 receptor gene in melanoma cells deﬁne structural determinants of receptor function doc

Ngày tải lên : 17/03/2014, 10:20

... to the responsive HBL cells The lack of functional coupling of these cell lines, particularly of DOR cells homozygous for the wild-type MC1R, is therefore perplexing and will be the subject of ... lines, and that impairment of signaling through the cAMP cascade occurs in these cells even in the absence of mutations in the coding region of the gene Finally, our study of the natural MC1R variants ... suggests that the Leu93Arg mutation has no effect on the processing of the receptor molecule, at least as studied with the tagged forms of the protein Taken together, these results show that the efﬁciency...

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báo cáo hóa học:" Activation of human B cells by the agonist CD40 antibody CP-870,893 and augmentation with simultaneous toll-like receptor 9 stimulation" doc

Ngày tải lên : 18/06/2014, 15:20

... capacity of B cells Effect of3 Effect of CP-870,893 on T cell stimulatory capacity of B cells Purified CD19+ B cells from each of donors were stimulated as described in Figure 2, irradiated, then ... immune surveillance and immunotherapy are enhanced in the absence of B cells [15-19], potentially due to the elimination of suppressive or regulatory B cells [18,20] B cells have been shown to be ... B cells, CD19+ CD27+ B cells, or CD19+ CD27neg B cells were incubated in a 5% CO2 incubator at 37°C in 96-well round-bottom plates at a concentration of 105 cells/ 100 ul in the presence of either...

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Báo cáo y học: " The functional expression of extracellular calcium-sensing receptor in rat pulmonary artery smooth muscle cells" potx

Ngày tải lên : 10/08/2014, 05:21

... FI of [Ca2+] i has decreased, it is still higher than control group Furthermore, NPS2390 also decreased the FI of [Ca 2+ ] i However, the elevation of Figure Effect of various inhibitors on the ... contributions All authors participated in the design, interpretation of the studies and analysis of the data and review of the manuscript B-FY, L-YW, RW and C-QX conducted the experiments C-QX supplied critical ... for 30 at 37°C in the dark The cells were rinsed three times with Tyrode’s solution to remove the remaining dye, and they were further incubated in Tyrode’s Li et al Journal of Biomedical Science...

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Báo cáo y học: "Anti-Inflammatory mechanisms of the proteinaseactivated receptor 2-inhibiting peptide in human synovial cells" ppt

Ngày tải lên : 10/08/2014, 05:21

... and then trypsin for another 30 minutes The relative levels of p-p65 were calculated by normalizing the band densities to that of GAPDH and setting the controls as 100% Chen et al Journal of ... (B) Cells were pretreated with PAR2-AP at different concentrations for 30 minutes and then incubated with trypsin at 30 nM for hours The addition of trypsin to the cells, pretreated with the ... synergistically induce COX-2 [18] Recently, the expression of COX-2 was shown to be induced by the activation of PAR-2 through bacterial infection, or the treatment of either trypsin or PAR2-AP, and mediated...

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Báo cáo y học: "Expression of the α7 nicotinic acetylcholine receptor in human lung cells" pdf

Ngày tải lên : 12/08/2014, 18:21

... hamster PNEC cells [6] PNEC cells are one of the possible cells of origin for SCLC [11,12] The expression of the α7 nAChR is not an artifact of cell culture since the expression of the α7 nAChR ... [15], supporting the hypothesis of ubiquitous expression of the α7 nAChR receptor mRNA in human lung cells In addition, we have demonstrated expression of the α7 nAChR protein of the correct molecular ... pronounced upregulation of the α7 nAChR in the lungs of the newborns [24] Together these data indicate that the α7 nAChR may play an important role in the development of SCLC, and other lung cancers...

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Báo cáo y học: "Diagnostic utility of the soluble triggering receptor expressed on myeloid cells-1 in bronchoalveolar lavage fluid from patients with bilateral lung infiltrates" pps

Ngày tải lên : 13/08/2014, 08:21

... included 68.4% of patients with a CPIS of more than 6, compared with 49% of patients in Gigot's study It therefore seems that a CPIS of more than is not an efficient factor in the diagnosis of pneumonia ... 11,000/mm3 (4) within 24 hours of administration of the initial antibiotic therapy or immunosuppressive therapy before BAL In total, 42 patients were excluded because of previous treatment with nonspecific ... intensivists were unaware of the results of sTREM-1 measurements in BAL fluid On the basis of clinical, radiological, and microbiological data, patients were assigned to one of three groups (Table...

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Báo cáo y học: "Inclusion of the glucocorticoid receptor in a hypothalamic pituitary adrenal axis model reveals bistability" ppt

Ngày tải lên : 13/08/2014, 16:21

... synthesis and release of cortisol (O) Cortisol (O) regulates its own synthesis via inhibiting the synthesis of CRH (C) in the hypothalamus, and ACTH (A) in the pituitary The equation for the ... synthesis and regulation in the pituitary (Figure 1) We have augmented this model by including synthesis and regulation of the glucocorticoid receptor (R) in the pituitary [18,19] In the pituitary, ... 0

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Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"

Ngày tải lên : 03/11/2012, 11:17

... active PKC may exist in the nucleus of melanoma cells, and the presence of active PKC isoforms in the nuclei of many cell types has been confirmed (10-12) Staining of the G361 cells with antibodies ... signal (CLDN1-NLS), there is increased expression of claudin-1 in the nucleus, but there are still large amounts of claudin-1 in the cytoplasm as well B) All of the claudin-1 in the nucleus can be ... analysis, these transfectants demonstrate the presence of claudin-1 protein in the cytoplasm, with small amounts in the nucleus When cells are transfected with CLDN1 under the control of a nuclear...

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Sensibility study of flooding and drying issues to the operating conditions in PEM fuel cells

Ngày tải lên : 05/09/2013, 14:58

... decrease of the current density Moreover, there are also cases where the current increases in a first part of the channel, reaches a maximum, and then decreases further along the channel In these ... distribution in the cell (Figure 6) Figure Influence of the inlet cathode relative humidity on the water content in the membrane and on water vapor profile in the electrodes at the center of the cell ... Effect of the temperature The cell internal humidification is thermo-dependent as a consequence of the water saturation pressure thermo-dependency The critical current will therefore depend on the...

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Tài liệu The Electrical Properties of Cancer Cells pptx

Ngày tải lên : 14/02/2014, 22:20

... 1999) The concentration of minerals in the ECM, the composition of proteoglycans, the molecular weight of the proteoglycans, the amount of bound water in the ECM, and the pH of the ECM control the ... improve the electrical functions of the ECM Therefore the composition and degree of toxicity of the ECM-glycocalyx interface will affect the electrical field and the flow of biocurrents in the ECM The ... removal of old and damaged cells and in stimulating the growth of new cells Fibroblasts and fibrocytes are the main cells that produce the proteins and ground substance of the ECM in soft tissue The...

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Tài liệu Báo cáo khoa học: The pro-form of BMP-2 interferes with BMP-2 signalling by competing with BMP-2 for IA receptor binding pptx

Ngày tải lên : 18/02/2014, 06:20

... with its receptors [16] In the case of BMP-9, the pro-peptide appears not to alter signiﬁcantly the activity of the mature part [17] For the pro-peptide of BMP-7, a targeting role to the extracellular ... complex of the pro-peptide and BMP-9 can bind to the type I receptor Alk1 [17], the latencyassociated polypeptide of TGFb inhibits the interaction of TGFb isoforms with type II and III receptors ... BMP-2 for binding to BMP receptor IA (BMPR-IA), one of the main receptors of mature BMP-2 [6,27,28] In contrast, the ECD of BMPR-II was not bound by proBMP-2 Furthermore, the free propeptide formed...

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