the vapor pressure of a liquid depends on the temperature of the liquid it also depends on
Báo cáo khoa học: On the mechanism of a-amylase Acarbose and cyclodextrin inhibition of barley amylase isozymes pdf
Ngày tải lên :
08/03/2014, 08:20
... AMY1 and AMY2 active sites
are twice as long as that of the human and porcine enzymes
containing only five subsites [9–12]. In addition, a noncata-
lytic site that facilitated adsorption onto starch ... corresponding
dissociation constants. In this equation, it was easier for a
calculated constant to compare its value relative to zero
rather than to obtain a large value. When the association
constant value was ... spectra of acarbose binding
The inhibition of the amylolytic activity of AMY1 by
acarbose involved, as shown above, specific interactions at
either the active site, as in EI, and/or at the secondary
binding...
- 9
- 437
- 0
Guidance on the teaching of writing skills INSET opportunities for teachers of a subjects across the curriculum at Key Stages 2 and 3 docx
Ngày tải lên :
10/03/2014, 05:20
... attractive formats. A list of
useful software can be found on the websites of the British Dyslexia Association
and the Professional association of teachers of students with specific learning
difficulties ... as writers, they demonstrate that they can:
ã engage their reader(s)
ã adapt their writing to suit the audience and purpose of the piece
ã use grammatical and stylistic features to ensure clarity, ... Over the past 10 years, the gap between boys’ and girls’ performance has
increased, with the widest gap in writing.
ã The content of the writing of many learners of all abilities is often marred...
- 174
- 616
- 0
Tài liệu Báo cáo khoa học: Application of a fluorescent cobalamin analogue for analysis of the binding kinetics A study employing recombinant human transcobalamin and intrinsic factor pdf
Ngày tải lên :
19/02/2014, 05:20
... rough
comparison of the dissociation velocities indicated at
least a 10-fold faster liberation of the fluorescent ana-
logue when compared with Cbl. The CBC dissociation
spanned at least 90% of the total ... respects. An alternative
explanation seems to be more probable. Thus, distor-
ted shape of the analogue causes partial corruption of
its bonds with IF. As a consequence, the ligand and
the protein ... °C was incubated over time. Free ligands
were adsorbed on charcoal, and the absorbance spectra
were recorded. Concentration of appearing IF–CNCbl was
calculated by comparison with the standards...
- 12
- 603
- 0
Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
Ngày tải lên :
19/02/2014, 06:20
... TCA TCA
TCA TCA TCA GGG CAC GAC ACA CAT CCC C; and
reverse primer, GAT CGG TAC CTC ATT ACA GAG
GAG GGA TAT GGG GAA C. The PCR reaction was
done as described above. The amplified PCR product was
inserted ... by
MALDI-TOF ⁄ TOF fragment ion analysis as well as
by Edman degradation of the corresponding peptide
after purification by reversed-phase chromatography.
During the search for and confirmation of ... was amplified from genomic T. reesei DNA
with the following primers: forward, GGG GAC AAG
TTT GTA CAA AAA AGC AGG CTA TCA TGC TGT TGT
CAG GTC CCT CTC G; and reverse, GGG GAC CAC
TTT GTA CAA GAA...
- 14
- 650
- 0
Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
Ngày tải lên :
21/02/2014, 00:20
... acid affinity of the Paratelphusa lectin was
further proved by its inability to inhibit sialidase-treated
BSM and bovine thyroglobulin. Also the lectin failed to
agglutinate desialylated rabbit ... purification.
Erythrocyte preparation
Blood for HA assay was prepared as described by
Ravindranath et al. [12].
Hemagglutination assay
Hemagglutination assays were performed in microtiter
plates (Falcon) as recommended ... of animal glycoconjugate. They act
as an important component of the ligands recognized by the
lectins. Recognition can be affected by specific structural
variations and modifications of sialic acids...
- 8
- 616
- 0
Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
Ngày tải lên :
22/02/2014, 04:20
... 5Â ATA CAC ACA AAC TCA CAA GG 3Â
MWU 5Â CAA CAT ATT TTA AAT AAA ATT GAC AAT 3Â
C9LM* 5Â ATA AAA ATG GTA TAC CTC ACT TGT*C 3Â
C9UM1 5Â TAA CAA TGA TTC TCT TTC TTT TAG 3Â
C9LM1 5Â AAT CTT TAT TTT ... TGG CAC AAC ATT GTA CAT TCT TGG G 3Â
C9U 5Â GTA TGG AAG TGT GGA ATA GTT T 3Â
C9L 5Â GGA CAC CAC AGA TAT CCA ATA G 3Â
C1011U 5Â CAT AAA ACT AAC AAT ACA TGT 3Â
C1011L 5Â TAG CAG ATA TTG AAA AGG AG ... amplication
betweenC7UandC8L.Primersinitalicswereusedinthegenotyping
of allele M.
C1U
5Â GAG AGG AAC TGA ACA GAA CAT TG 3Â
C1L 5Â CAA CTG CGT ATT AGT GAA GAA TG 3Â
C2U 5Â AAT CAA ATT TTA TTA TAA GAC C 3Â
C2L
5Â AAT AGC TAA TTA GAG ACC AT 3Â
C3U...
- 11
- 568
- 0
Tài liệu Báo cáo Y học: Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase pdf
Ngày tải lên :
22/02/2014, 04:20
... steric
clashes with the aromatic ring of Tyr269, and these
unfavorable interactions could lead to a decrease of local
flexibility and an increased E
a
value.
The validity of the above interpretation was ... value almost 2.5-fold higher than
the native cold adapted enzyme (Table 1). The mutant
G26 1A/ Y26 9A exhibits an E
a
almost the same as in the case
ofthenativeenzyme(Table1).
Thermal inactivation ... was further
reinforced by the construction of the double mutant
G26 1A/ Y26 9A. The additional substitution of Tyr269 fi
Ala was designed with the aim of reducing the spatial
constraints originating...
- 6
- 488
- 0
Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
Ngày tải lên :
07/03/2014, 11:20
... DR2:
5Â-CCGTAAGGTCACAAGGTCACTCG-3Â,DR3:5Â-CCG
TAAGGTCACAGAGGTCACTCG-3Â, DR4: 5Â-CCGTAA
GGTCACAGGAGGTCACTCG-3Â, DR5: 5Â-CCGTAAGG
TCACCAGGAGGTCACTCG-3Â. PAL0: 5Â-CGCAAGGT
CATGACCTCG-3Â. One strand of each oligonucleotide was
annealed after ... [21]. A SmNR1 specific probe of 497 bp was
produced by PCR amplification with TOPO 2.1-SmNR1 as
a template (forward primer: 5Â-ATTTCAGAAGTTGAAC
AAACACAC-3Â, reverse primer: 5Â-AAGATGGTATT
GAAGATGATGGTTGA-3Â), ... bind to the Gal1 UAS
element and the Gal4 activation domain will drive transcription of
the expression of the reporter gene. Individual AH109 yeast
colonies obtained from initial transformation were...
- 16
- 542
- 0
Báo cáo khoa học: O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner doc
Ngày tải lên :
08/03/2014, 02:20
... step of fatty acid
metabolism, i.e., the reaction of fatty acid with CoA to
produce acyl-CoA on the outer membrane of mitochondria.
Acyl-CoA is then transported into the matrix for
b-oxidation of ... within the rat olfactory
placode. Recombinant O-MACS protein tagged with c-Myc
and His6 demonstrated an acyl-CoA synthetase activity for
fatty acid activation, and protein localization to mitochon-
dria ... Yoshihara, Y., Kawasaki, M., Tamada, A. , Fujita, H., Hayashi,
H., Kagamiyama, H. & Mori, K. (1997) OCAM: a new member of
the neural cell adhesion molecule family related to zone-to-zone
projection...
- 10
- 393
- 0
Báo cáo khoa học: Characterization of a high-affinity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus ppt
Ngày tải lên :
08/03/2014, 02:21
... in
ligand recognition. However, the fact that the iodinated
ligand is still biologically active and displays an affinity
comparable with the affinity of the native peptide indicates
that the tyrosine ... biologically active. Binding of this ligand to the
microsomal fraction of S. oryzae extract was found to be
saturable and reversible, with an a nity (K
d
)of2 .6n
M
,and
a high maximal binding capacity ... susceptibility
or resistance of the
Sitophilus
spp. strains
The specific binding activity of the microsomal fraction of
different weevil strains, of the pea aphid A. pisum and of the
fruit fly D. melanogaster...
- 7
- 604
- 0
Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the specific and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf
Ngày tải lên :
17/03/2014, 03:20
... water. The incorporation of
18
Ofromradio-
labeled water was not observed with guaiacol.
It was clear that the b-aryl ether cleavage enzyme
catalyzed the addition of two molecules of H
2
O(atCa and
Cb ... presence of guaiacylglycerol (GG) and 4MU
(data not shown).
Localization of enzymatic activity
To confirm the localization of the b-aryl ether cleavage
activity, we prepared a hyphae fraction (HP), an ... incorporated into GG. In
addition, we found the radiolabeled oxygen at the Ca and
Cb positions in a comparison of the mass spectrum with
that of the products of the reaction with GG and
unlabeled water....
- 10
- 670
- 0
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