... me - at the tower. So there was another person out there, on the roof of the tower. But the person in the garden was not the ghost of the woman. It was little Miles. ... stood on the roof of the towetThere were two towers, one at each end of the roof. Each tower had a room inside, and you could climb out onto the roof from them; Flora ... strange or dangerous places - the roof of the tower, the other side of the lake. It's dangerous but exciting, for Flora and Miles. They'll try to get to those...
... cRBCs. Defining the glycanspresent on the surface of cRBCs will allow either for the design of strategies to optimize the agglutination assayor the design of alternative strategies for the detectionand ... by the label and independent ofthe attri-butes ofthe glycan to which it is attached. Accord-ingly, we aimed to determine the amount of individualcRBC glycans by HPLC through the use of a ... value of approxi-mately 2.00, confirm the presence of two protons.Notably, there is no indication of presence of extracross peaks at 4.70, which would represent the Glc-NAc portion of a lactosamine...
... antibodies to the subunits ofthe yeast bc1complex indicated on the left side ofthe blots.1212 V. Zara et al. (Eur. J. Biochem. 271) Ó FEBS 2004Further insights into the assembly ofthe yeast ... relatively unaffected. Therefore, the absence of subunit 6 appeared to alter the rates of processing of two of the redox subunits and caused minor changes in amounts of the small supernumerary ... transmembrane helix of subunit 8 and helices G and H1 of cytochrome b andbetween the N-terminus of subunit 8 and helix a of cytochrome b. The figure was constructed from the crystal structure ofthe yeast...
... Control analysisofthe las operonFEBS Journal 272 (2005) 2292–2303 ª 2005 FEBS 2293minute. The relative values of simultaneous modulation of the three las enzymes are calculated as the average of ... up-regulation[16] ofthe las operon at low PK activity and a polareffect ofthe pyk deletion on expression of ldh.Animportant question is whether this affects the conclu-sions drawn from our control analysis. ... were related to the A280 of the extract, for the purpose of determining relative activities. The specific activ-ities of PFK and PK and LDH in MG1363 were deter-mined as UÆmg)1 of protein, where...
... are the only prized possessions the couple has. Everything is now set up for the rest ofthe story to unfold.ConflictDella sells her hair. The conflict is supposedly the moment where the "problem" ... gets the money to buy Jim a great present, eliminating the first problem through decisive action. Shortly thereafter she finds the perfect present, so neither the money nor the present is the ... "problem" in the story appears, but this story began right from the first with a problem. In "Gift ofthe Magi" the point of conflict actually solves the first problem and...
... collection in the region (Sheldon et al 2008). One ofthe key positive unintended outcomes ofthe EHMP was the building of momentum leading to the establishment ofthe Partnership. The EHMP began ... increased by 71%, amounting to 23% ofthe State’s direct output from the industrial sector. Mining employed 3.2% ofthe local labour force during the period. The offices ofthe major mining companies ... The second part ofthe report puts forward a critique of a program that targets one ofthe priority issues that emerged from the conceptual framework analysis – the role ofthe Healthy Waterways...
... [29]. The overall analysis, examining the topology ofthe phylogenetic tree and the position of each gene inside the cluster, was informative regard-ing the orthology relationships ofthe distinct ... enzymes. The most significant difference between the amphi-oxus and the other retinal reductases was, nevertheless,their preference for the NADH cofactor. To ourknowledge, these are the first ... FEBSTo analyze the contribution of protein domains to the ER anchorage, the localization ofthe full-lengthenzyme was compared with those of five truncatedforms. The pattern ofthe full-length...
... reflects the differentsizes ofthe fusion proteins. Immunological analysis revealed the presence of Pex6p in the precipitate of full-length Pex1p–ProtA and also the presence of Pex1p in the full-length ... comprising the N-terminal fragment and the first AAA-cassette of PEX6 (PEX6N + D1–GAL4-BD). Neither ofthe cas-settes alone nor the two AAA-cassettes together or the N-terminal part together with the ... respectively[11,12]. These two peroxins bind their cargo in the cytoplasm, transport them to (and possibly across) the peroxisomal membrane and return to the cytoplasmfor the next round of import. The membrane-boundsteps...
... some ofthe op-tions the Navy might consider.This report should be of interest to the Office ofthe Secretary of Defense and to Navy policymakers involved in the present and futuredirection of ... depending on the extent ofthe maintenance. The air-craft then returns to the fleet for another 40 months. This cyclerepeats itself until the aircraft retires. Approximately 8 percent of the aircraft ... Thus, many parts ofthe airframe have shorterlives than other parts ofthe airframe. The overall life ofthe E-2C isabout 10,000 hours, which is driven by the life limit ofthe wing cen-ter...
... world, although these are unlikely to return to the heights ofthe late 1990s any time soon. Production of Hardware, Peripherals and other ICT goods There are other components of ICT for development ... world, based on the dominant trends of recent years, which recognizes an essential interplay of complementary forces: the prominence ofthe private sector, the enabling role ofthe public sector ... mechanisms. They have in common a recognition ofthe vital importance of and opportunities for ICT in the pursuit ofthe Millennium Development Goals, and focus their efforts on bringing together...
... of landscape, the number anddesignofturbines,thepattern of their arrangement, their color, and the number of blades.Visual or aesthetic resources refer to the natural and cultural features of ... proposalforthe exploitationofthe world’soffshorewindresourcewas designed for the eastern coast ofthe United States in the early 1970s (72).This use of a very large resource has not been pursued in the ... turbines use hinges on the hub thatallow the blades to move into and out ofthe plane of rotation independently of each other. Because the blade weights may not balance each other, other provi-sions...
... Impact ofthe Abolition ofthe Milk Quota Regime – Regional Analysis ofthe Milk Production in the EU" (AGRI-2007-0444). The project aimed at a thorough policy impact analysisofthe EU ... through either the transfer of an entire farm, the leasing or purchase of quota, or the allocation of quota from a national reserve. The transfer of milk quota may take place via a variety of administrative ... average, with +35.5% in the EU-10 and +13.8 in the EU-15. This excess supply makes the net trade position ofthe EU-27 stronger towards the Rest ofthe World. Within the EU there is a quite heterogeneous...
... calculate the position ofthe footprint. Letters to the right of eachpanel indicate the DNA sequence (5¢ end in the upper part) ofthe corresponding strand in this region. In the lower part, the sequence ... towards the 3¢ end ofthe topstrand and the 5¢ end ofthe bottom strand (Fig. 4B).This agrees with the presence of a larger residue thatmakes stable contacts with this region of DNA. The interaction ... footprint. Letters to the right of each panel indicate the DNA sequence (5¢ end in the upper part) of the corresponding strand in this region. Below the footprints, the sequence ofthe corresponding...
... and the C1sprotease ofthe C1 complex of complement [25]. Thus, the Trp band can be regarded as a characteristicfingerprint ofthe native fold of PSP-I and PSP-II. The near-UV CD spectra ofthe ... lgÆmL)1solution of 125I-labelled PSP-I ⁄ PSP-II at pH 4 resulted in broaden-ing ofthe peak and the appearance of species at the elution volume ofthe isolated subunits (Fig. 3C). The addition of Zn2+at ... the absence (s)orin the presence of 0.5 (n)or4(h)mM Zn2+. The continuous lines correspond to the fit ofthe experimental data to a sigmoidal function.Table 1. Thermodynamic parameters of the...
... min; then 10 lLofincreasingconcentrations of PCI or ProNtPCI were added and the measures were continued for 2 min. The slope of the first part ofthe assay corresponded to moand the slope of the ... [18,19]. The extremelyinefficient folding of PCI in vitro [18], together with the presence ofthe above-mentioned long pro-sequences,suggest a possible involvement of them in the in vivo folding of ... investi-gated the role of both pro-sequences in the in vitro refolding of PCI, together with studies ofthe influence of the N-terminal pro-region on its in vivo expression. Both studiesconclude that the...