... a ⁄ b domain than in thea domain [20].This is also reflected in the rigidity ofthe protein. The atomic displacement factors ofthe PhyK structure aresmaller for thea ⁄ b domain than for the ... coli phytase, glucose-1-phosphatase and human prostatic-acid phosphatase. The polypeptidechain is organized into an a and an a ⁄ b domain, and the active site islocated in a positively charged ... substrate-freeAppA the C a atoms are 2.41 A ˚apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1.87 A ˚.Distinct conformational changes were observed...
... that the calcium-binding event of lower affinity is attributable to the catalytic site of PLDa2, as found for PLDb from A. thaliana [39]. The decrease in PLD activity at concentrationsabove the ... members of the PLD superfamily. However, there is low similar-ity in the remaining parts ofthe molecules. Most mam-malian PLDs contain a PX and a PH domain instead of the C2 domain in plants, ... Journal 274 (2007) 26302640 ê 2007 The Authors Journal compilation ê 2007 FEBS 2633 from the ratio ofthe forward scattering intensity of the sample and that ofthe molecular mass standard BSA. The ab...
... at the same time, distinct absorption bands of oxyhemeappeared at 540 and 579 nm. Then, a broad bandappeared at around 660 nm, and was maximal 9–12 minafter initiation ofthe reaction. The ... vanishedafter 30 min, and instead, an absorption peakappeared at 637 nm, suggesting the formation of CO–verdoheme. The 637 nm band disappeared gradu-ally and was replaced by a new broad band ... band maxima ofthe azide-freeform at 405 nm and ofthe azide-bound form at421 nm, so the relative amounts ofthe nonbound andazide-bound forms were estimated directly from the values of absorbance...
... assets to arrive at the ‘all-in’ fee as a percentage of plan assets. Also, each plan s total dollar fee amount was divided by total participants in theplan to arrive at the ‘all-in’ fee as ... In the report, Exhibit 3 plots the impact of average account balance and number ofplan participants on the ‘all-in’ fee for a variety of combinations of average account balance and number ofplan ... assigned to an individual plan is:Probability of such aplan in the Form 5500 plan universeProbability of such aplan in the Deloitte/ICI plan sample for the asset/participant cell that the...
... dimerization ofthe bc1complex atthis stage (i.e. just after the addition of ISP and Qcr10p).On the other hand, in the transition from the 500 kDaband to the 670 kDa band, a structural rearrangement of ... in the molecular mass ofthe bc1complex inthese two deletion strains, thus leading to the hypothesisthat the addition of ISP may play a pivotal role in the structural rearrangement ofthe ... were of analytical grade.Yeast strains and growth media The genotypes and sources ofthe S. cerevisiae strains aredescribed in Table 2. The ISP deletion strain wasprepared in accordance with the...
... SPRK also has an aspar-tic acid residue at position at 200, and with the sameconformation as in the two other structures, butAsp200 forms a salt bridge to Lys253 (Ala253 andAsn250 in PRK and ... [18]. The biophysical characteri-zation of SPRK did not reveal the classical cold adap-ted features [19], but still initial comparative studiesshowed that the catalytic turnover was at least ... reported.Results and discussionCrystallization, data collection and refinementCrystals ofthe catalytic domain ofthe Serratia prote-inase K (SPRK) grew within a few days to thin hexa-gonal plates growing...
... system. Also, their underlying task (air travel domain) is much simpler than our tutoring task. In addition, the SIH is not always available and users have to activate it manually. Other visual ... hints/de-scribes the answer, each discourse segment has an additional purpose annotation that is displayed when the segment is part ofthe visible horizon. Auto-collapse. To reduce the amount of infor-mation ... also have an equivalent in a new domain (e.g. displaying the recognized user answer can be the equivalent of the correct answers). Other NM usages can also be imagined: e.g. reducing the length...
... status, and clinical variables such as cancerstage at diagnosis, time after diagnosis, and initial treat-ment. All measures will be collected at the beginning of the trial, and at 6, 12, 18 and ... possible for them to be blinded to the group assignment. The participants on the otherhandareunawareofthegroupassignment,astheyareunder the assumption that the hospital is providingusual care.Intervention ... Providinginformation that i s congruent with a patient ’sneedsatthat particular time is an important determinant forpatient satisfaction and affects health-related quality of life (HRQoL) and anxiety and...
... Hospital and Regional Medical Center, Seattle, WA, USAProtein phosphatases are primarily classified on the basis ofthe type ofthe amino acid they dephosphory-late, serine ⁄ threonine phosphatases ... fused to an EF-hand motif, and human STP(HsSTP), which has an additional 8 kDa a- helicaldomain at the C-terminus [3,4]. The sizes ofthe catalytic domains of both PPP andPPM families are well ... kinase and adenylosuccinate syn-thase, but not in S. agalactiae CovR ⁄ CsrR. The motifis located at the surface ofthe SaPPase (Rantanen,unpublished), and superposition ofthe prolines allowssuperposition...
... excluding the INS at xiobtained atStep 3 from the ANS at xiand thereby we can identify the true interaction structure at xi(Supplementary material,Supplementary mathematical descriptions, Theorem ... however,only applicable to the case when for each node there areas many parameters as the number of overall networknodes and these parameters do not directly affect the corresponding node. The fundamental ... chosenn parameter perturbations. All these approaches arebased on parameter perturbation experiments whichare, however, not always achievable in many practicalcases.In this paper, we therefore...
... to an increase in the rate of the initial fast phase, i.e. oxidation ofthea chains. The rates of oxidation are reduced in the presence of chelators of heavy metal cations in most cases. An ... 3A. The rate of auto-oxidation of cross-linked Hb was greaterthan that of Hb A 0,both in the presence and absence of EDTA, as has been observed previously [17]. In addition, the rates of auto-oxidation ... binding ofthe Hb tetramer to the LPSmolecule makes the heme cavity ofthea chains moreaccessible to a water molecule which can then accelerate the displacement ofthe protonated superoxide anion,...
... demonstrate the presence of positive charge.Figure 4I shows the bottom face (same as in Fig. 4C) of a van der Waals model ofthe best structureof RIIa D/D. The central part of this face appears ... two basic sites, the N-terminalamide and His2. Aspartic acids Asp27 and Asp30 showlower mean pK a values than their model pK a values. Thismay be a general property of Asp residues, as it has ... Toexamine the electrostatic character ofthestructureof RIIaD/D we have predicted mean apparent pK a values, based onPoisson–Boltzmann electrostatic calculations, using anensemble of calculated...
... compilation ª 2009 FEBS 2831 ofthe alanine ofthe AXXCH motif (Ala25) (ingreen) and the unsaturated vinyl group ofthe heme(cyan) are separated by 3.41 A ˚(as compared with a typical thioether ... 72% similarity. The structuresare remarkably similar overall, and in the details of the heme attachment and heme position. The rmsdbetween thestructureof C. fasciculata cytochrome cand S. ... Crystallographic data were collected atbeamline IO2 at Diamond Light Source, UK, and weacknowledge the support of T. Sorensen at Diamondand A. Labrador at the ESRF, France.References1 Barker...