the standard form of a complex number is
Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
... B=MICBị=nwhere A and B are the MICs of drug A and drug B in the combination, MIC A and MICBare the MICs of drug A and drug B alone, FIC A and FICBare the FICs of drug A and drug B and n is the number of ... & Batoni G (2008) Comparative analysis of the bactericidal activities of amphibian peptide analoguesagainst multidrug-resistant nosocomial bacterial strains.Antimicrob Agents Chemother 52, ... thank M. Simmaco for use of the facilities andplatforms available in the DiMA Unit of the Sant’An-drea Hospital. This work was funded in part by ItalianMinistero dell’Universita`e Ricerca...
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Tài liệu Issues Involved When Updating the Primary Key of a Parent Row pptx
... are also made in the child DataTable. This is the default. None Indicates that no action takes place. SetDefault Indicates that the DataColumn values in the child DataTable are to be set to the ... Cascade; therefore, when you change the DataColumn in the parent DataTable on which the ForeignKeyConstraint was created, then the same change is also made in any corresponding DataRow objects in the ... to the database. By default, DeleteRule is set to Cascade; therefore, when you delete a DataRow in the parent DataTable, any corresponding DataRow objects in the child DataTable are also deleted....
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Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc
... chloroplast spinach GAPDH [35] w ere examined t oAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcus119119119119119120119121118119159159159159157158157160158159199199199199197198197200198199VI ... oAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcus119119119119119120119121118119159159159159157158157160158159199199199199197198197200198199VI ... a glutamic acid residue affects t he kinetic parameters of the R197E mutant. The K0.5forBPGAistwicethatofthewild-type recombinant GAPDH and the catalytic constant is onehalf with NADPH as cofactor....
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... mutants) and per-manganese metal basis (manganesesuperoxide dismutase mutants).Table 2. Metal contents of mutant superoxide dismutases. Metal con-tent was measured by atomic absorbance and is ... purchased fromPharmacia Biotech (Vienna, Austria).Visualization and analysis of molecular structuresMolecular structures whose coordinates were obtainedfrom the RCSB database were visualized ... Peplow, F. Yamakura and T. Matsumoto for the analyses of iron and manganese in protein samples. We also wish tothank H. Steinman for the gift of E. coli OX32 6A. We finally thank MrM. Farrugia for...
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Tài liệu Báo cáo Y học: The insert within the catalytic domain of tripeptidyl-peptidase II is important for the formation of the active complex potx
... variants of TPP II ( i.e. rat,mouse, fruit fly, Arabidopsis thaliana, Caenorhabdit is elegansand Schizosaccharo myces pombe), and at least three humanEST-clones covering this area (GenBank a ... standard assay and the i mmunoreactivity was detected by Western blot analysisand quantitated as described i n Materials and methods. Open andfilled circles indicate the activity, and open and ... catalytic His264, and the proximity to the active site may explain the effect of oligomerization onenzyme activity. Even though the exact mechanism for complex formation and activation of the...
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Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Bifidobacterium longum and its complex with fructose pot
... the axisarea are of polar character. The water channel in the apo form of the enzyme runs along the axis of the b-propeller through the whole domain and is onlyblocked by Cys236 in the area ... probably a consequence of a mistake incorporated by the TaKaRa Ex TaqÔ poly-merase and did not affect the enzymatic activity.MS The molecular mass of the recombinant b-fructofuranosi-dase was ... performs a nucleophilic attack on the anomeric carbon of fructose. The leaving group is carboxylate of Asp54.Dimerization The asymmetric units of the crystals of both the apoand complexed form...
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Báo cáo khoa học: The propeptide in the precursor form of carboxypeptidase Y ensures cooperative unfolding and the carbohydrate moiety exerts a protective effect against heat and pressure pot
... indicating that the thermalunfolding process of the precursor form follows a two-statetransition. The ratio of the unfolding enthalpy (DHcal)tothevan’t Hoff enthalpy (DHv) was 1.05 (DHcaland ... stabilization of proCPYagainst pressure.In contrast to the two-state transition of the precursor form, mature CPY showed a multistate transition: a firsttransition in the 0.1–150 MPa range, a ... or was obtained from Oriental Yeast Co. (Lot21003805) (Osaka, Japan) and proCPY was prepared as the same manner as CPY, with minor modifications. Dgly CPYand Dgly proCPY, in which the asparagine...
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Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx
... occurrence of a different type (type I) of fatty acid biosyntheticpathway in the human host from that of the malariaparasite, make this pathway an attractive target fordeveloping antimalarial agents ... for the analysis of the conformational stability of other acyl carrier proteins.AbbreviationsAAS, acyl-ACP synthase; ACP, acyl carrier protein; AcpS, holo-ACP synthase; apo-ACP, Plasmodium falciparum ... form and the holo form displayed a reversible two-state unfolding mechanism. The analysis of iso-thermal denaturation data provides values for the conformational stability of the two proteins. Although...
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Báo cáo Y học: Incorporation of 3-nitrotyrosine into the C-terminus of a-tubulin is reversible and not detrimental to dividing cells potx
... significantly to the disappear-ance of nitrotyrosinated tubulin, the tyrosination/detyrosin-ation at the C-terminus of a- tubulin is the main operatingmechanism. Furthermore, when cells maximally ... dysfunction.ACKNOWLEDGEMENTSWe thank Drs Carlos E. Argaran˜ a and Mario Guido for criticalreading of the manuscript, Mrs S. N. Deza and Mrs M. G. Schachnerfor technical assistance and Dr Stephen Anderson ... Secretarı´adeCienciayTe´cnicadelaUniver-sidad Nacional de Co´rdoba y Agencia Co´rdoba Ciencia del Gobiernode la Provincia de Co´rdoba, Argentina.REFERENCES1. Barra, H.S., Arce, C .A. ,...
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Báo cáo khoa học: Common mode of DNA binding to cold shock domains Crystal structure of hexathymidine bound to the domain-swapped form of a major cold shock protein from Bacillus caldolyticus pot
... formation at the terminator site. When the adjacent uracil-rich sequence is being transcribed, the affinity of the dA–U base pairs is insufficient to stabil-ize the DNA–RNA duplex, and the transcript,together ... implicated in transcriptional activation andrepression, regulation of alternative splicing, regula-tion of mRNA stability, translational activation orrepression and RNA packaging. It was also shownrecently ... sugar–phosphate backbone of the nucleotide in subsite 6 and the formation of the alternative subsite 7 are a consequence of the inaccessi-bility of Trp8 in this crystal form. In addition to their...
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Báo cáo khoa học: The crystal structure of a hyperthermostable subfamily II isocitrate dehydrogenase from Thermotoga maritima pdf
... D36N-15-CATCCTTCCCTATCTCAACATCCAGCTGGTTTACT-3D341N-15-AAGGGGAGAACTCAACGGAACACCGGAGG-3D389N 5-CACTCTCGAAGAGTTCATAAACGAAGTGAAGAAGAATCTC-35-GAGATTCTTCTTCACTTCGTTTATGAACTCTTCGAGAGTG-3M. Karlstroăm ... 5’-CCTGGAGAAATCGATCATGAGCTTCGCTCAGTCGTG-3’5’-CACGACTGAGCGAAGCTCATGATCGATTTCTCCAGG-3’F205M 5’-AAAAGGTCGACATCTGGATGGCGACGAAAGACACGATC-3’5’-GATCGTGTCTTTCGTCGCCATCCAGATGTCGACCTTTT-3’D36N ... different conformations, the large and the small domains of each homologue were comparedseparately. The rmsd values between the C a -carbons of the large domain of TmIDH and that of PcIDH andHcIDH...
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Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt
... protozoa, CCT has been characterized at the genesequence level in the ciliate Tetrahymena pyriformis [19,20],in the diplomonadide Giardia lamblia and the parabasilideTrichomonas vaginalis [21]. ... nondenaturing PAGE; the ratios of labeled to unlabeledtubulins are indicated on the top of each lane and the arrow indicates the position of the b-tubulin/CCT complex. (B) The amounts of the tubulin ... CCT were quantitated by the use of a phosphori-mager and expressed as a fraction of the maximum amount of boundlabeled tubulin and plotted as a function of the ratio of labeled : un-labeled tubulin.Ó...
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The preference of a Female Greek island population in regard to the gender of their gynecologist pdf
... a rather unimportant factor. The younger age during the first visit and the regular breast exam are positively correlated with the choice of a male gynecologist. Other parameters, such as ... JOURNAL đ VOLUME 4, ISSUE 1 (2010) using as a dependent variable the potential preference of a male gynecologist, the age of the first visit and the breast exam presented, in comparison to the ... social relationships. According to the literature, the patients believe that a female gynecologist is more compassionate and largely possesses the so called humanitarian values, whereas there...
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Making sense of a complex world Accounting for royalty arrangements – issues for media companies pptx
... licensee Is the obligation a financial / monetary liability?Advance payment may be made to licensor Is the asset a financial/monetary asset? Is the value of the asset fully recoverable?Development and ... releasing the advance to the income statement as revenue only as the publisher generates actual sales, rather than recognising in full as under the rst example. The assessment of an advance ... operations rather than manage the design, manufacture, shipping and sale of team branded merchandise. Teams therefore outsource this function to a third party, typically a sportswear manufacturer....
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Báo cáo khoa học: Escherichia coli cyclophilin B binds a highly distorted form of trans-prolyl peptide isomer doc
... the case of CyPB in complexes with Suc-Ala-trans-Pro-Ala-pNA and Ac-Ala-Ala-trans-Pro-Ala-AMC, the largedistortion in the orientation of Ala1 and Ala2 of Ala-Prowith //w torsional angles of ... using a 32-mer primer of 5Â-AAAAAGAATTCATCGATATGTTCAAATCGACC-3Â with EcoRIadded at the 5Â end of ClaI, a 23-mer primer of 3Â-CGAGACGGCATTCCTAGGTTTTT-5Â, and pATtrpEPPIa as a template. The P ... [5,17,18,26–29] andshorter peptides such as Ala-Pro, Ala-Pro-Phe, Ala-Ala-Pro-Ala and Ala-Ala-Pro-Ala-Ala i nhibit cis to transisomerase activity of calf thymus CyPA [27]. It wassuggested that in the...
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