the spatial orientation of zebrafish intestinal epithelium renewal by negative marking of epithelial cells
THE SPATIOTEMPORAL STUDY OF ZEBRAFISH INTESTINAL EPITHELIUM RENEWAL
... epithelium renewal Chapter The spatial orientation of zebrafish intestinal epithelium renewal The spatial orientation of zebrafish intestinal epithelium renewal by positive marking of epithelial ... Chapter The spatial orientation of zebrafish intestinal epithelium renewal 30 The spatial orientation of zebrafish intestinal epithelium renewal – by positive marking of epithelial cells ... expression of these genes in the intestinal epithelium of zebrafish at a level of single cell The main aim of this thesis is to produce a detailed study and analysis of the zebrafish intestinal epithelium...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... 20 μg of CII/20 μl of PBS in the right ear and with 20 μl of PBS in the left ear DTH response was calculated as the percentage swelling (the difference between the increase of thickness of the ... right ear and the left ear, divided by the thickness of the left ear, multiplied by 100) Data are expressed as the mean (standard error of the mean) Differences were analyzed by the Mann-Whitney ... demonstrated by the addition of inhibitors of these enzymes - GW274150 and indomethacin [8,36], respectively - to the co-cultures The addition of these inhibitors resulted in the abrogation of the inhibition...
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Retrovirology Research BioMed Central Open Access In vivo expression of the HBZ gene of HTLV-1 doc
... normalize the HTLV-1 tax or HBZ mRNA expression level per provirus, the mRNA/DNA ratio was calculated by dividing the HTLV-1 tax or HBZ mRNA load by the HTLV-1 proviral load Neither the HBZ (C) nor the ... ATL, HCs – ATL) The zero value of tax gene transcripts was observed in 60.7% of HAM/TSP patients (34 out of 56), 71.1% of HCs (27 out of 38) and 30.0% of ATL patients (3 out of 10) The medians are ... significant numbers of individuals tested (60.7% of HAM/TSP patients, 34 out of 56; 71.1% of HCs, 27 out of 38; 30.0% of ATL patients, out of 10), whereas HTLV-1 HBZ mRNA was expressed in all the infected...
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Báo cáo y học: " The connection domain in reverse transcriptase facilitates the in vivo annealing of tRNALys3 to HIV-1 genomic RNA" potx
... 1A, and are named according to the number of amino acids deleted from the C terminus of GagPol Figure 1B shows Western blots of lysates of the viruses produced from the different transfections, ... packaging tRNALys3 incorpora- tion into HIV-1 is not affected by deletion of the IN domain in GagPol, nor by further deletion of the RNaseH Page of (page number not for citation purposes) Retrovirology ... Figure 2B The left side of panel A shows that there is a linear increase in the reverse transcription signal over an almost 10 fold change in the amount of BH10.P- viral genomic RNA used in the reac-...
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Báo cáo sinh học: "A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma" ppsx
... upstream of the Luc cDNA, and both genes were separated by a linker sequence of 33 nucleotides All transgenes were expressed under the control of the CMV promoter The correct sequence of the fusion ... for the Luc expressing U87MG cell clone was 125 cells We further examined whether the cytotoxic activity of the HSV-TK moiety could be visualized by monitoring light emission from intact cells ... generated by enzymatic conversion of prodrug by HSV-TK was however not measured in either of the above studies Ponomarev et al [24] did not present data on the correlated expression of the three...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
... respectively The bioavailability of free EPI was 100%, the BAR of PA/EPI calculated through the equation above by the AUC0–24h was 189%, much higher than the free drug According to the previous ... control of the particle size (Zhang et al., 2009) The size and the size distributions of PANs and PA/EPI in distilled water were measured by DLS The morphological characteristics were observed by ... major contributor for the clearance of NPs, thus the reducing rate of MPS uptake could be considered as the best strategy for prolonging the circulation of NPs (Li & Huang, 2008) Therefore, PA/EPI...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... in rabbits by the Scientific Services Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at ... estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing determined amounts of the purified UP12 were separated by SDS/PAGE ... mimicked the effect of GroES by releasing almost all of the polypeptides from GroEL After treatment with ATP and 0.1% DM, these polypeptides were recovered in the top three fractions of the sucrose...
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Báo cáo khoa học: In vivo production of catalase containing haem analogues pot
... PP The concentrations of porphyrins used in the growth medium are given in Table In the case of Ru-meso, a low concentration had to be used because of the toxicity of this compound Production of ... suggested that the metal ion of the porphyrin is removed during transport of the porphyrin from the medium to the catalase protein in the cytoplasm Alternatively, but less likely, the metalloporphyrin ... 700 nm (C) Spectra of catalases produced in the presence of Cu-PP, Mg-PP, and PP The intensities of these spectra have been normalized with respect to the Soret band absorption The inset in (C)...
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Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt
... operation of the network However, the response for a specific system is constrained by the connection between these elements The response is also influenced by the relative dominance of these individual ... where, KS is the half saturation constant for activation of Gal3p by galactose and t refers to the total component concentration The expression of GAL genes is determined by the binding of the operator ... validate the mechanism of induction of GAL genes by galactose In each of the models, cytoplasmic Gal3p is activated by galactose Further, Gal4p dimerizes and interacts with the DNA to form the DNA–Gal4p...
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Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx
... modifications of chromatin [9] Little is known, however, of the functional role of the remaining subclass members In this study, the yeast protein SPP1 (alias cps40), a member of the COMPASS complex ... constitute CGBP homologues The presence of a TFS2M domain is also indicative of a possible function for the DIDO1 long isoform (Figs and S2) It is the second domain of the elongation factor, TFSII ... instability is the most probable explanation for the lagging chromosomes in DIDO1-targeted cells Targeting of the DIDO1 locus leads to genomic instability, as shown by the occurrence of lagging chromosomes...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
... sensitization by degrading the airway epithelial barrier, as it was found to disrupt tight junctions and increase permeability in confluent monolayers of epithelial cells [15] All these activities ... from the administered [75Se]Der p2, indicate together with its own remaining intact levels (the 16 kDa band) that the clearance and further metabolism of the allergen was altered as a result of the ... conclude that the inflammatory state of the lung influence on the rate of metabolism and clearance of Der p Thus, an allergic response may lead to prolonged retention of the allergen in the airways...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... of their crystal structure of Pisum sativum PSI, in which the loop of PSI-G is suggested to face the stroma The amount of PSI-G in the transformed knock-out line does not reach the level of the ... topology where the loop is in the thylakoid lumen, and the N-and C-termini face the stroma, opposite to that of PSI-K On the other hand, the second scenario implies a stromal loop, like that of PSI-K, ... integration of the subunit into the PSI complex A protein band smaller that the tagged versions of PSI-G is present in the lanes with the loop-tagged PSI, but is also seen faintly in the lanes with the...
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Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
... analyzed by the Levene test; in those cases in which the variances were unequal, the data were adequately transformed before the ANOVA The null hypothesis was accepted for all the values of these ... A-deficient rats In the liver of rats with hypervitaminosis the results showed a decrease in the amount of p53 and an increase of the c-Jun The figure shows that the expression of p53 is time course ... position, the amplified DNA were resolved by agarose electrophoresis The intensity of fluorescent dye, ethydium bromide, relative to the intensity from the input gives the enrichment generated by the...
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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx
... effects of EBI compounds on the plasma membrane H+-ATPase of U maydis Furthermore, the utilization of two different targets in the same biosynthetic route permitted us not only the confirmation of the ... in the indirect effects of sterol modification, namely, the activation of plasma membrane H+-ATPase through changes in the fatty acid profile There have been numerous reports on the influence of ... sterols plus, in the case of EBI-treated sporidia, other collateral effects of these compounds [33] In U maydis, changes in the plasma membrane 18 : 2/18 : ratio followed the inhibition of growth rate,...
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Báo cáo khoa học: A study of microRNAs in silico and in vivo: bioimaging of microRNA biogenesis and regulation doc
... the fluorescence dye from the quenching molecule by the Dicer enzyme, which cleaved the synthetic let-7 precursor miRNA (Fig 1D) [19] In the absence of the Dicer protein, the emission energy of ... general, the functional actions of miRNAs in mammalian cells, unlike plant cells, are associated with the translational inhibition of target mRNAs by binding miRNA to the seed region of the target ... abnormalities occurring during each of the steps of miRNA biogenesis Second, the oncogenic characteristics of the miRNAs regulate the progression of cancer by preventing the tumor suppressor genes from...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
... aorta The level of HSP90 and the type of NOS isoform present in rat brain and aorta were determined by immunoblotting (Fig 1) In brain, nNOS was the most preferentially expressed isoform, together ... in these digestion processes was demonstrated by the protective effect on both HSP90 and NOS degradation of the administration to the HSD-treated NMS animals of C.I.1 (Fig 5) In the brain of ... [28,42] d The loss of calpastatin activity was calculated by subtracting the sum of the percentage of the active calpastatin species from 100 HMS Control + HSD + HSD + C.I Fig Levels of calpain...
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báo cáo hóa học:" In vivo properties of the proangiogenic peptide QK" pdf
... published by the National Institutes of Health in the United States (NIH Publication No 85-23, revised 1996) and approved by the Ethics Committee for the Use of Animals in Research of "Federico ... daily The concentration is reported as the final molar concentration in the organ bath Endothelium-independent vasorelaxation was tested after mechanical endothelium removal of the endothelial ... http://www.translational-medicine.com/content/7/1/41 Figure Diagram 3of the kinetics of wound closure (A) Diagram of the kinetics of wound closure (A) VEGF165 and QK accelerate the closure of full thickness punch biopsy wounds...
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Báo cáo hóa học: " In vivo evidence of htid suppressive activity on ErbB-2 in breast cancers over expressing the receptor" potx
... to the logarithm of the initial amount of the target DNA in the sample The relative concentration of one target with respect to another is reflected in the difference of the cycle number, the ... point of view very attractive, the analysis of the correlation of the expression levels of the two forms L and I in the tumor samples not support it at present Decrease in the level of the L ... sample) The SD of ΔΔCt is the same as the SD of the ΔCt The ΔCt values of both the tumor and normal samples are standardized to HGPRT used as endogenous control The values defined as increase of expression...
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báo cáo hóa học:" Function of anterior talofibular and calcaneofibular ligaments during in-vivo motion of the ankle joint complex" docx
... position of AJC motion There was no bony interference with the path of the ATFL and CFL The position of the AJC during MR scanning was defined as the neutral reference position The length of the ATFL ... study the average length of the ATFL was 15.8 ± 2.9 mm and the average length of the CFL was 27.7 ± 2.7 mm in the neutral position The study of Burks and Morgan measured the length of the longest ... distances between the centroids of the digitized attachment areas on talus, calcaneus and fibula Once the position of the AJC was reproduced in the modeling software, the length of the ATFL and CFL...
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báo cáo hóa học:" In vivo evaluation of a vibration analysis technique for the per-operative monitoring of the fixation of hip prostheses" pdf
... and therefore on the final strength of the bone-implant structure Therefore the per-operative characterization of the primary stem-femur contact and the assessment of primary stem stability in the ... more intricate deformation modes of the femur in combination with deformation modes of the prosthesis In the case of simple bending modes of the femur the prosthesis stem acts just like an added ... insertion, and the tightness of clamping was thus the same for all FRFs i = n) In the measured structure the only variable was the connection between the implant and the bone The shaker was held by a member...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008