the site of amputation
Báo cáo y học: "Mechanical complications and reconstruction strategies at the site of hip spacer implantation"
... promotes the emergence of high local antibiotic concentrations due to the continuous friction of the articulating components Cement debris can be then easily removed at the time of the prosthesis ... removal, and insertion of a cement-coated modular prosthesis with a spacer head The cement mantle of the prosthesis is also antibiotic-loaded according to the sensitivity profile of the causative organism ... Femoral fractures at the site of hip spacer implantation should be treated when an unstable joint situation results, the outcome of the surgery is endangered or the mobilisation of the patient is hereby...
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báo cáo khoa học: "Failure of functional imaging with gallium-68DOTA-D-Phe1-Tyr3-octreotide positron emission tomography to localize the site of ectopic adrenocorticotropic hormone secretion: a case report" ppsx
... MRI of her pituitary gland once again did not reveal the presence of an adenoma Given the failure of biochemical or imaging techniques to localize the site of excess ACTH secretion, medical therapy ... source of ectopic ACTH, the role of clinical judgment plays a significant role Ongoing monitoring of the patient, combined with a relevant discussion of risks and benefits of different therapeutic ... Thus there is a need for a combination of tests to help determine the cause of Cushing’s syndrome Furthermore, functional imaging may not always assist in localizing an anatomical site of excess...
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Báo cáo y học: " Immune status in sepsis: the bug, the site of infection and the severity can make the difference" docx
... However, although the authors report differences, these differences were minimal Only community-acquired pneumonia was associated with a higher number of NK cells as compared with the other groups, ... associated with an enhanced number of CD8+ cells The latter group was the only one with a significantly enhanced number of apoptotic CD8+ cells Reduced expression of HLA-DR on CD14+ cells was seen ... sepsis were compared with those with severe sepsis and septic shock, the number of significant differences in terms of the number of circulating NK cells, CD4+ T lymphocytes, CD8+ T lymphocytes, and...
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
... molecules of the asymmetric unit of the wild-type structure Neither the mutation nor the different crystallization conditions evoked structural differences The crystal was grown in the presence of phytate, ... well The hydrogen bond between the backbone nitrogen atom of Asp291 and the 3phosphate of the substrate is the only interaction with the protein backbone; all other contacts are formed using the ... optima in the acidic range They consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic site at the interface of the two domains [4,5] HAPs can initiate hydrolysis of phytate...
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Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
... of the aglycone-binding site, probably subsite +1, because they influence the binding of the first glucose unit of the aglycone On the other hand, M453 may be positioned in subsite +2 because these ... influence on the glycone binding of the Sfbgly interactions with the aglycone Thus, the effect of the alteration of the glycone structure in the stability of the ESà complex (DDGà) for the wild-type ... purposes, the glucose unit forming the non-reducing end of the aglycone was called the ‘first glucose unit’ and the other glucose units were sequentially named in the direction of the reducing end of the...
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Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx
... and 235 [9] Of theses residues, only Arg213 is in the vicinity of the active site of RTA In order to establish the role of arginyl residues around the active site of RTA in the binding of rRNA, ... C1 of the hexanucleotide is at the bottom left and the target adenine is at the top of each model The sidechains of RTA are shown in blue (A) Model has the tetraloop bound in the active site of ... and, as such, these mutants could not be purified To assess the effect of substitutions made at each of the arginyl residues on catalytic activity of RTA, the ability of each of the purified RTA...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... as a consequence of the mutagenic replacements Effects of the His120Asn and His145Asn mutations on the affinity of metal binding to arginase II To further examine the effects of the His120fiAsn and ... for the differences between the active sites of this enzyme and arginase I In spite of the relatively low agmatinase activity of the Asn149Asp variant, it is clear that the interactions of arginase ... inactive in the absence of added Mn2+ At the optimum pH, the Km for agmatine (2.5 ± 0.2 mm) was very close to the Km of the wild-type arginase for arginine However, the hydrolytic activity of Asn149Asp...
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Tài liệu Báo cáo khoa học: Site-directed mutagenesis of a loop at the active site of E1 (a2b2) of the pyruvate dehydrogenase complex A possible common sequence motif docx
... detected by the PDH assay The interaction of E1 (a2b2) with the PSBD was unaffected by any of the mutations in E1a (Fig 4) Therefore, any effects of the mutations on the catalytic activities of the PDH ... properly as a lid for the active site, thereby granting easier access for the cofactor ThDP to its binding site The lower thermal stability of the mutant E1s and the increased Vmax in the DCPIP assay, ... affecting the efficacy of the recognition of the tethered lipoyl domain by E1, an essential prelude to reductive acetylation of the pendant lipoyl group [5,9,39] The damaging effects of mutations in the...
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Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc
... indicated by the MD simulations of the modelled ternary hGK–Glc– ATP complex In the final structure of the simulations, the interactions of the adenosine moiety were similar to those observed in the binary ... ATP-binding site in the MD simulated model structure of the binary hGK–ATP complex and the domain motion induced by ATP binding to the hGK apoenzyme (A) Close-up view of the ATPbinding site in the MD ... active site loop and partial closure of the interdomain active site cleft The modelled structure of the hGK–ATP binary complex shows the residue contacts involved in ATP binding at the active site...
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Báo cáo khoa học: Interactions of imidazoline ligands with the active site of purified monoamine oxidase A potx
... the I2BS stereospecificities would be useful Interestingly, the lowest-energy orientation for each of the three ligands in the large active site of MAO-A put them in the middle of the active site ... and the values for 2-BFI and idazoxan are given in Table Evidence for the proximity of these ligands to the flavin in MAO-A comes from the influence of the ligand on the redox properties of the ... observed The transfer of electrons from the amine to the flavin requires a catalytic interaction, so the nitro˚ genous group of agmatine must be within A of N5 of the isoalloxazine ring of the flavin...
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Báo cáo khoa học: Site-directed mutagenesis of selected residues at the active site of aryl-alcohol oxidase, an H2O2-producing ligninolytic enzyme pot
... 50% of them clustered together in front of the rectus (re)-face of the isoalloxazine ring of the FAD cofactor This substrate location is shown in Fig 2A, which includes the 10 molecules of veratryl ... with the hydroxyl group in Ca, representative of the range of AAO substrates [9,19,33] Most of these alcohols docked in front of the re-side of the isoalloxazine ring of FAD [34], with the benzylic ... oxidase, the flavin domain of cellobiose dehydrogenase and cholesterol oxidase The positions of the conserved histidine and histidine ⁄ asparagine residues near the FAD isoalloxazine ring of four of the...
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Báo cáo khoa học: Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain doc
... opposite end of the barrel to the active site has weaker electron density and the temperature factors of the ˚ atoms in this segment are 31 A2, 2.35 times above the average B for the structure The ... formed between the enzyme and Tris are the same as observed previously [16] In the Glu-A complex there are two acarbose molecules: one in the active site and the other on the sur˚ face of the enzyme ... however, they stack nicely against the aromatic rings of Tyr351 and Trp139, respectively The distances between the sugars and the aromatic rings of ˚ the two residues are 4 A The mode of acarbose...
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
... positioned in the ÔupÕ position rows and shows that in the presence of the substrate, rotation of Asp142 towards Glu144 lowers the pKa of the latter by 0.8 pH units The calculated effects of the D140N ... for the position of residue 142 [34] The pKa calculations indicate that the only candidate for a residue determining the basic arm of the pH-activity profile of the D142N mutant is Glu144 The ... these residues are not described well and there is no example of mutational analysis of all of these residues in the same enzyme To obtain an insight into how the many conserved residues in the...
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![Báo cáo khoa học: Assignment of the [4Fe-4S] clusters of Ech hydrogenase from Methanosarcina barkeri to individual subunits via the characterization of site-directed mutants pdf](https://media.store123doc.com/images/document/14/rc/do/small_dor1394202044.jpg)
Báo cáo khoa học: Assignment of the [4Fe-4S] clusters of Ech hydrogenase from Methanosarcina barkeri to individual subunits via the characterization of site-directed mutants pdf
... [20] The midpoint potentials of these clusters are pH independent Hence, the properties of the iron–sulfur clusters present in the catalytic core of complex I seem to differ from those of the ... for the enzyme from the EchF8 mutant in Fig To determine if the mutations had turned the spin of the ground state of the cluster(s) of subunit EchF to S ¼ ⁄ 2, EPR spectra were recorded in the ... below 15 K a twofold splitting of the Nia–L signal was observed due to the interaction of the Ni-based unpaired electron with the S ¼ ½ system of the reduced proximal [4Fe-4S] cluster The temperature...
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Báo cáo khoa học: Local changes in the catalytic site of mammalian histidine decarboxylase can affect its global conformation and stability pptx
... radius of the protein, as the major diameter of the dimer is predicted to be given by the distance between the carboxy termini of both monomers (from the lower left to the upper right of Fig ... HDC catalytic center at the interface between the monomers (Fig 1), as is the case of DDC [18] One of the monomers (monomer A) would contain the major part of the catalytic site pocket, including ... postulated the hypothesis that the observed high degree of rotation of the torsion angle v induced by the presence of the substrate could produce a remarkable conformational change in the whole...
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Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf
... required for the correct orientation and stabilization of the deprotonated thiolate anion in the active site of the enzyme [20] On the basis of protein sequence similarity, active site residue ... Lys117 and Val172 in the GSTU2 isoform, all of the mismatches being located in the H-sites of the enzymes [11] As long as the enzymes were expressed in vitro and purified, they exhibited different ... as the substitution of the chlorine atom with the more electronegative fluorine in the CDNB molecule did not affect the kcat values Altogether, these results are consistent with the idea that the...
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Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf
... that these residues stabilize the distortion of the NAG3 residue In these interactions, we particularly focused on the Trp664 residue, which is located at the bottom of the active site cleft The ... conformational changes, but of the removal of negative charge at these residues Conformation of chito-oligosaccharides bound to the active site cleft For the structural determination of the AD2–substrate ... Stereo figures of the model of the bound substrate in the AD2 E526A mutant (A) and D524A mutant (B) The structures of bound sugars and the side-chains of three acidic residues in the conserved...
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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt
... glucose of the bglucan substrate in the )1 subsite at the bottom of the active site pocket [20] The entrance to the active site pocket of Exg is flanked by a pair of phenylalanyl residues, Phe144 ... that the Phe144:Phe258 pairing that lines opposite sides of the entrance to the pocket was particularly relevant because it largely defined the +1 subsite Indeed, the importance of the +1 subsite ... seen for the Phe triad in the structure of the mutant Exg:L3 complex Neither of the two sugar residues interacting with the Trp triad overlaps with the sugar seen in the Phe-Phe clamp of Exg Structural...
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Báo cáo khoa học: Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center? Supporting amino acid residues at the active site of penicillin G acylase pptx
... group in the proximity of the oxyanion can be found in the rest of the Ntn hydrolases [3] From another point of view, the hydrogen bond between the oxyanion and the aNH3+ of SerB1 resembles the one ... chain carbonyl group of AsnB241 The main chain carbonyl group of GlnB23 closely interacts with the oxygen atom in the locality of the nucleofuge in the structural analog of the TI These experimentally ... [15] On the other hand, the hydrolysis rate varies with the substituent on the amino moiety of the substrate, confirming that the formation of acyl enzyme (AE) is the rate-limiting step of the reaction...
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Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot
... features of the consensus motif into the Zm-p60.1 scaffold, an analysis of structural and catalytic properties of the mutants, and simulations of the substrate–enzyme interactions of the wild-type ... analysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the two modes of aglycone binding The amino acid sequence of Zm-p60.1 was compared with the ... cytokinin-O-glucosides are natural substrates for both of the two b-glucosidases, Zm-p60.1 and Bgl4:1, but the architecture of their sites that recognize the aglycone moieties of these substances differs distinctly,...
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