... epitopes locatedin the MUC5AC C-terminal cysteine-rich part.Further localization ofthe epitopes of 45M1,2-12 M1 and 166M1In order to further locate the epitopes ofthe hithertounmapped 45M1, ... detailedmap ofthe epitopes for the mAbs, where 45M1 recog-nizes an epitope located in the N-terminal region of the C-terminal cysteine-rich part of MUC5AC, pre-sumably in the last CysD domain ofthe ... mucous cells ofthe epithelia lining the stomach and the trachea, where it constitutes a major component of the gastric and respiratory mucus. A number of mAbs have been raisedagainst the gastric...
... Impact ofthe Abolition ofthe Milk Quota Regime – Regional Analysis ofthe Milk Production in the EU" (AGRI-2007-0444). The project aimed at a thorough policy impact analysis ofthe EU ... through either the transfer of an entire farm, the leasing or purchase of quota, or the allocation of quota from a national reserve. The transfer of milk quota may take place via a variety of administrative ... average, with +35.5% in the EU-10 and +13.8 in the EU-15. This excess supply makes the net trade position ofthe EU-27 stronger towards the Rest ofthe World. Within the EU there is a quite heterogeneous...
... between the projection ofthe tilt ofthe z axis on the xÂ,y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location ofthe rhombic (x, y) axes. The orientation ofthe ... at position E7 as the source ofthe H-bond to ligand on the basis of apartial sequence, which had indicated a Tyr on the distalE-helix. The similarity in the 1HNMRspectraofthethree globin ... ligand.Comparison ofthe strengths of dipolar interactions insolution with the alternate crystal structures of methemo-globin establish that the solution structure of wild-type Hbmore closely resembles the...
... demonstrated the role of PufX in the orientation ofthe RC inside the LH1Fig. 4. Isolation ofthe PMCs on a sucrose gradient. The final detergentextracts from the ICM were loaded on the top of a 10–40% ... PufXD2)7neither affects the photo-synthetic capability ofthe bacteria nor the efficiency of exchange ofthe ubiq uinol molecules between th e RC and the bc1, a s judged from the reduction kinetics of ... residues ofthe LH1 apolypeptide in the organization ofthe LH1–RC complex[12]. In the present work, we have investigated the possible involvement ofthe N-terminus and of the C-terminus of PufX...
... as seven a-helical transmembrane domains traversing the lipid bilayer. The enlarged circle shows the amino acid sequence ofthe distal N-terminus of the V1aR and indicates the position of Arg46 ... with the corresponding sequence of the V1aR [10]. The role ofthe N-terminus has beenaddressed recently by alanine-scanning mutagenesis of the N-terminus ofthe V1aR; this revealed that a ... mutation of Arg46 had the dualeffect of (a) decreasing agonist affinity and (b) promoting the agonist-induced active conformation due to the loss of astabilizing constraint on the ground state of the...
... those ofthe parent protein, andinvestigated the exchange kinetics ofthe correspondingdimers. The P19A mutation slightly increases the thermalstability ofthe monomer, but it does not alter the ... evaluation ofthe effect of singlepoint mutations on the solution structure of monomericderivatives, we analysed the 2D NMR spectra of the different variants of mBS. Figure 3 shows the expandedregions ... swappingtendency ofthe dimer. In contrast, the L28Q mutation sig-nificantly affects both the dimerization and swapping pro-cesses but not the thermal stability ofthe monomer. Overall,these results...
... copies ofthe N protein, respectively. (C) The RNA binding efficiency of N protein is represented as a percentage of bound RNA (i.e. the percentage of bound RNA = 100%)percentage of free probe). The ... phosphorylation.Effect of RS motif phosphorylation onoligomerization ofthe N proteinTo better understand the effect of RS motif phosphor-ylation on the biological function ofthe N protein, weFig. 3. The translation ... that of the full-length N protein (data not shown). Taken together,these data suggest that the RS-rich region ofthe SARS-CoV N protein is possibly phosphorylated by SRPK1.Phosphorylation of...
... and showed, in particular, the configuration of ManNAc to beD.From the combined the data on the structures of the isolated oligosaccharides, the overall structure of the P. frisingensis LPS carbohydrate ... with 7a (Table 1) because ofthe b-effect ofthe acetylation. Acetylation of 7b was confirmed byESI MS data, which gave the expected mass of 3221.4 Da. The spectra of oligosaccharide 6 were ... 3-O-methyl-6-deoxytalose, as setting the L-isomer instead of the D-isomer resulted in the disappearance ofthe contactbetween protons U6 and P4.To determine the absolute configurations ofthe mono-saccharides,...
... FAD/NADPH domain(% 16-fold) (29). However, the domain mixture still recon-stituted only % 2% ofthe activity ofthe full length CPR. InthecaseofNR1,thekcatfor the full length enzyme is low(1.3 s)1) ... enzymes, the potentials for the FAD/NADPHdomains are rather more negative than those for the FMNdomain, reflecting the direction of electron transfer fromNADPH through FAD, then FMN and on to the ... BM3 [28]. Aggregation ofthe NR1FAD/NADPH domain was much less extensive in the absence of cofactor reduction.Stopped-flow kinetic studiesReduction ofthe FAD/NADPH domain of NR1 wasinvestigated...
... LPS)[5,6]. The serological specificity ofthe bacteria is definedby the structure ofthe O-specific polysaccharide chain(O-antigen) ofthe lipopolysaccharide. On the basis of the O-antigens, the strains ... the Dconfiguration (the b effect on C3 of L-GlcAwouldbeclosetozero[27]).On the basis ofthe data obtained, it was concluded that the repeating unit ofthe O-polysaccharide of P. myxofac-iens has the following ... 2.01–2.10.These data together suggest that the tetrasacchariderepeating unit ofthe polysaccharide consists of two residues of GlcNAc and one residue each of GalNAc, GlcA andAlaLys. The 1Hand13C...
... reports and transmit them to the Commission, within six months ofthe end ofthe year to which the reports relate.’ REPLY OFTHE COMMISSION60Special Report No 9/2012 – Audit ofthe control system ... is the responsibility ofthe competent authority ofthe Member State to verify the competence ofthe control body to issue a certificate of inspection, and in particu-lar the evidence that the ... send these supervision reports. There were no reports from Austria, for example. At the end ofthe audit (November 2004), of the 48 reports due over the period 2000–03 in respect ofthe 12...
... amounts ofthe ECDs ofthe non-a sub-units of human muscle AChR for use as starting material for the determin-ation ofthe 3D structure ofthe receptor ECDs and for the characterization of the ... datafrom electron images ofthe AChR found in largeamounts in the electric organ ofthe marine ray,Torpedo californica [3]. The acquisition ofthe crystallo-graphic structure ofthe mollusc acetylcholine-bindingprotein ... domain [3]. The N-terminal ECD of each ofthe two a subunits con-tains the major part ofthe binding site for the cho-linergic ligands. The two sites are nonequivalent, onebeing formed at the interface...
... buildings owe their design to the necessities of parties, witnesses and their lawyers attending court for hearings, and, behind the scenes, the needs ofthe judges and ofthe back offices, which ... Parliament, the executive, and, of course, the justice system. 10. Reform ofthe justice system, to our courts and the legal profession, like reform ofthe law, must clearly be consistent with the ... Sir Anthony May, the recently retired President ofthe Queen’s Bench Division put it recently, the fabric of justice . . . is part ofthe fabric of society.7’ The fabric of justice demands...
... in the U.S. devoted to education, but there is no substantiation of this figure, and most ofthe blogs discuss are professional or academic blogs. There is an extended discussion of nonprofit ... that the traditional methodological approaches, and their traditional points of focus, lack relevance today. However, the complexity ofthe changes taking place and the shifting nature ofthe ... outlining the historical context of health and media studies, the authors describe major individual-level theoretical frameworks applicable in the area: social cognitive theory, the theory of reasoned...