... divisors of n, Ω(n) is the number of prime power divisors of n, π(x) is the number of primes ≤ x, τ(n) is the number of divisors of n. P(s, t) is the set of positive integers composed of prime ... is the set of square-free members of P (s, t).We list a few estimates from prime number theory and sieve theory. The first is the Brun-Titchmarsh inequality and the second is a consequence of the Prime ... author enjoyed the hospitality ofthe Institute of Mathematics and Informatics, Bulgarian Academy of Sciences. Finally, the author acknowledges the referee for a thorough reading ofthe paper and...
... of a4v over the entire sequence defines the aggregationprofile ofthe polypeptide. The hot-spot threshold(HST) was defined as the average ofthe a3v ofthe 20natural amino acids weighted by their ... [36].Thus, there are three scales which allow the predic-tion of amyloidogenic regions in a protein sequence (or rather, the ability of a peptide to be amyloido-genic): the scale ofthe packing ... propensity enter the scoring functionas a physical property term Sphyspropconsisting of the sum ofthe products ofthe amino acid frequency with the normalized property value ofthe respective...
... at the last base, G to A, abolished the interaction of HIPPI, so the last motif would notinteract with HIPPI. The other two motifs might be the target of HIPPI. In the upstream sequenceof the Table ... confirmed the in vitro resultthat mutation ofthe motif abolished the binding of HIPPI to the specific sequenceofthe caspase-1 geneupstream sequence, and the increased expression of caspase-1 ... decrease in the fluorescence intensity of 6X(HN)-pDED due to the addition ofthe 60 bp region ofthe caspase-1 geneupstream sequence was observed. Addition of the caspase-1 gene upstream 60 bp sequence...
... localization in the ER [19]. We found that the TMD of UGT1A pro-teins appended to the C-terminus ofthe ectodomain of CD4 plasma membrane glycoprotein was able to retain the chimeric protein in the ER ... together, these data indicated that the TMD domain of UGT1A was sufficient to retain the ectodomain of CD4 protein in the ER. Because the carbohydrate moieties of proteins that are trans-ported to the ... in combination with that of the TMD did not prevent membrane targeting andinsertion ofthe enzyme. These findings resulted in the identification of an internal signal sequence localizedbetween...
... FEBSfungal sequences is clearly distant from the other bran-ches, which was indeed predicted. The other maingroup, obtained 97% ofthe time in 20 740 exploredtrees, contained the DIDO PHD sequences ... analyses The localization ofthe DIDO1 gene in the context of chromatin stability was addressed experimentally.Although the experiments conducted were preliminary,they indicated the involvement of ... from the CGBP family (Figs 1,3 and S1); otherwise, the fungal sequences could wellconstitute CGBP homologues. The presence of a TFS2M domain is also indicative of a possible function for the...
... however, the lower S/N ratio of the spectra in Fig. 4E than in 4B that has an effect on the accuracy ofthe deconvoluted spectrum.Lability ofthe Asn residues in the autoregulatory sequence The 28-residue ... neighbouramino acids and the loop structure are the same in the twoproteins. The perturbation ofthe overall conformation ofthe loopstructure as a result of deamidation of Asn32 to Asp/isoAsphave ... that atleast two ofthe labile Asn residues are located in the catalytic domain ofthe protomer [13]. Furthermore, on the basis of a nearest neighbour amino acid analysis of all the Asn residues...
... PpTGaseAlthough the overall identity with the mammalian TGaseprimary sequence is low in the deduced sequenceof the Physarum TGase, the middle region ofthesequence issignificantly conserved. The sequences ... shown. Datarepresent the mean of triplicate assays.Fig. 4. Phylogenetic tree ofthe full-length amino acid sequences of several TGases. The full-length amino acid sequences of severaleukaryote ... that the enzymaticactivity of TGase 4 was inhibited by the presence of GTP orATP, as in the case of PpTGase [51]. These results suggestthat the mechanism by which nucleotides inhibit the enzymatic...
... seen in the topological studies. The misfit dislocation ofthe pores interrupts the periodicarrangement ofthe pores. As another important result, the domains size is changed as a function of time(Fig. ... the ini-tial stages of anodization. However, it is assumed that the pits formed in the electropolishing contributing in the nucleation of pores on the aluminium in the order of 1010to 1012[18]. ... pores, the hexagonally ordering of pores is just achieved in the first stages of anodizing at the bottom of porous oxide layer and cannot grow upto the thick anodized layers. After removing the...
... highon the list of organisms whose genomes will be fullysequenced in the near future, the composition of the 43 kDa band would not have been revealed otherwise.Cloning ofthe two isoforms may ... areason for the different migration behaviour.Molecular cloning ofthe new hyaluronidaseisoform, Ves v 2bAiming at the molecular cloning ofthe cDNA of the newly discovered hyaluronidase isoform ... spectrum of a glycopeptide from the 43 kDaband. The masses of (glyco-)peptides are written vertically, those of free glycans horizontally, and that ofthe mother ion tilted. From the loss of two...
... min; then 10 lLofincreasingconcentrations of PCI or ProNtPCI were added and the measures were continued for 2 min. The slope of the first part ofthe assay corresponded to moand the slope of the ... [18,19]. The extremelyinefficient folding of PCI in vitro [18], together with the presence ofthe above-mentioned long pro-sequences,suggest a possible involvement of them in the in vivo folding of ... investi-gated the role of both pro-sequences in the in vitro refolding of PCI, together with studies ofthe influence of the N-terminal pro-region on its in vivo expression. Both studiesconclude that the...
... genome of CCHFV Kosova Hoti strainFigure 2 The protein analysis ofthe complete genome of CCHFV Kosova Hoti strain. A. Scheme ofthe M polyprotein of Kos-ova Hoti. B. Scheme ofthe L protein of ... nucleotide sequences of CCHFV fromKosovo have been published [9-12]. Three of them arepartial sequences of S segment, the remaining 2 representcomplete sequences of S and M segment of differentCCHFV ... during the epidemic in Kosovo in2001 [6]. The blood was taken on the 5th day after onset of symptoms. Results ofthe laboratory analysis showed the presence of IgM antibodies (titer 1:400) and the...
... techniquethat has helped facilitate the demonstration of these rela-tionships has been the use of dominant-negative mutantversions of one or more ofthe protein components of the MVB complexes [31]. Dominant-negative ... motif,although there were no further sequence similarities. P93is located in a sequence similar to the YP(x)nL motif, and the P329 and P332 residues were targeted because of sequence similarity to the ... deletion of the YPLGVG motif resulted in either less pronounced oran absence ofthe filamentous structures accompanied bynuclear localization ofthe mutant M protein. These datasuggest that the...
... cytomegaloviruses wasmost prominent in the central ~60% ofthe genome, with divergence ofsequence and lack of conserved homologs at the respective genomic termini. Of interest, the GPCMV genome wasfound ... comparisons of GPCMVORFs with homologous ORFs from the other sequencedCMVs are provided in additional file 3.In summary, the complete DNA sequenceof GPCMV wasdetermined, using a combination of sequencing ... cells are reminiscent of the mutations and deletions that occurred duringfibroblast-passage of HCMV [34] and rhesus CMV [35]. The congruence of these events suggests that the selectivepressures...
... andmicrofibrils in the outer layers ofthe fibre wall structure(Figure 1a). The surface structure of chemical pulpFigure 1 Structure of wood pulp fibres.(a) Note the network of microfibrils ... mole-cule, the elementary fibril, the microfibril, the macrofi-bril and the lamellar membrane. In the work of Maier[13], the term “ele mentary fibril” was reported to havea diameter of 3.5 nm ... respect to (1) the fraction of fibrillated fibres, (2) the fraction of nano-fibrils and (3) the morphology ofthe nanofibrils in anMFC material. Provided that a given MFC is composed of an appropriate...