the schwann cell response to peripheral nerve injury
báo cáo hóa học: " Sinomenine inhibits microglial activation by Ab and confers neuroprotection" doc
Ngày tải lên :
19/06/2014, 22:20
... to produce inflammatory and toxic factors that then affect the viability of neurons The second mode of neuronal toxicity is referred to as indirect toxicity Both kinds of toxicity mechanisms ... sinomenine-treated BV2 cells did not increase the number of TUNEL-positive cells (Figure 5B) These results suggest that the neuronal toxicity was mediated by factors released from the microglial cells after ... points to an important role for Ab in the pathogenesis of AD With regards to neurotoxicity, Ab can directly cause neuronal cell death (direct toxicity) or Ab can affect microglial cells to produce...
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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
Ngày tải lên :
22/02/2014, 04:20
... a-tocopherol (a-T) and T derivatives a-tocopheryl hemisuccinate (TS), a-tocopheryl acetate (TA) and a-tocopheryl nicotinate (TN) atherosclerosis, was further used together with LPS to strengthen ... 6.8) to dissolve cells was added to the cells, and then the cell suspension was sonicated in a bath-type sonicator for 5min The amount of protein of the solubilized cells was determined with a bicinchoninic ... UÆmL)1 were also added to the dishes for induction of NO production Then, 48 h after the addition of LPS/IFN, the cells were subjected to various assays Nitrite analysis The amount of NO was determined...
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Báo cáo khoa học: Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro doc
Ngày tải lên :
07/03/2014, 15:20
... overexpression of MT reduces the sensitivity of eukaryotic cells to oxidative injury [35] and the cytotoxic effects of NO [29] As both iNOS and MTs are induced at inflammatory sites by the same compounds ... factors in endothelial cells The metal release itself has been studied in cultured epithelial cells [26] Metallothionein has also been shown to protect eukaryotic cells from the cytotoxic and DNA-damaging ... were added to samples of 20 lM Cd5Zn2-MT2 to give final ratios as indicated at the bottom of Fig The normalized amounts of zinc and cadmium in the MT2 fraction, taking into consideration the dilution...
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Báo cáo khoa học: A pathway through interferon-c is the main pathway for induction of nitric oxide upon stimulation with bacterial lipopolysaccharide in mouse peritoneal cells pot
Ngày tải lên :
17/03/2014, 10:20
... in response to LPS was only marginally reduced in the adherent cell culture compared with that in the whole cell culture (data not shown) The IFN-c response of the adherent cells and RAW264.7 cells ... peritoneal cell culture The indirect pathway, through IFN-c, was indicated to play the major role in the peritoneal cell culture in the present study confirmed that the anti-(IL-18) Ig used in the ... on their cellular surface to transduce the signals inside the cells for production of IL-12, in a manner recently revealed as the usual way for LPS to induce a macrophage response [2,8] The cellular...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
Ngày tải lên :
30/03/2014, 04:20
... Laboratories) The procedure was made quantitative by the use of known amounts of proteins submitted to SDS-PAGE and staining with the appropriate antibody The bands were then scanned, and the areas ... order to explore the relationship between the HSP90 to NOS ratio and the formation of calpainresistant heterocomplexes, we first studied, by immunoprecipitation analysis, the association of the ... need to be addressed The first question concerns the vulnerability of different NOS isoforms to proteolysis in vivo under conditions of small changes to [Ca2+]i The second question concerns the...
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báo cáo hóa học: " Signaling pathways mediating a selective induction of nitric oxide synthase II by tumor necrosis factor alpha in nerve growth factor-responsive cells" pptx
Ngày tải lên :
19/06/2014, 22:20
... returned to cell culture medium Cells were allowed to recover for 24 hours before any treatments The cDNA coding for chimeric proteins bearing the extracellular domain of the TNFR1 receptor and the ... humidified cell incubator at 37°C kept at a 5% CO2 atmosphere Half of the medium was replaced every other day and the cells were split once a week to maintain cell viability Expression vectors Transient ... treatment, cells were transfected with either an empty vector or expression vectors for chimeric receptor proteins bearing the human TNFR1 ligand binding domains and the intracellular domain of either...
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Báo cáo khoa học: "Enhanced expression of constitutive and inducible forms of nitric oxide synthase in autoimmune encephalomyelitis" pdf
Ngày tải lên :
07/08/2014, 14:22
... of EAE, nNOS-positive cells were seen in neuronal cell bodies in the gray matter and in some inflammatory cells in the parenchyma of the spinal cord (4D) The eNOS-positive cells were found in vessels ... interaction between apoptotic cells and brain cells In rats with EAE, the majority of apoptotic cells were distributed in the parenchyma, but scarcely found in the perivascular cuffings of the spinal cords ... that the apoptotic cells were commonly found in the area adjacent to neurons (Fig 5A) and some GFAPpositive processes were identical to astrocytes (Fig 5B) In some cases, the apoptotic cells...
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Báo cáo khoa học: "Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development" ppsx
Ngày tải lên :
07/08/2014, 18:21
... ovary Expression of nNOS was detected in the surface epithelial cells (Fig 3A), stromal cells (Fig 3E), and the endothelial cells of blood vessels (Fig 3E) In the unilaminar, multilaminar, and antral ... in the ovary remains to be determined The findings, together with previous research, indicate that the expression of NOS is in part dependent on the stage of ovarian follicle development At the ... antral follicles were seen in the cortex (Fig 1A) From the interior to exterior, the multilaminar follicle was comprised of the oocyte, zona pellucida, granulosa cells, and theca (Fig 1B) Western blot...
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Báo cáo y học: "Protective effects of total fraction of avocado/soybean unsaponifiables on the structural changes in experimental dog osteoarthritis: inhibition of nitric oxide synthase and matrix metalloproteinase-13" docx
Ngày tải lên :
09/08/2014, 14:20
... shown to reduce the synthesis of the IL-1 receptor antagonist in chondrocytes [38], a process possibly responsible for the enhanced IL-1β effect on these cells Moreover, the diffusion of NO from the ... morphometric analysis The superficial zone of cartilage corresponds to the superficial and the upper intermediate layers, and the deep zone to the lower intermediate and deep layers The total number of ... disorganization) and pannus formation (scale to 3) The final score (range to 87) corresponds to the sum of the final scores for the three subregions of each specimen from the femoral condyle or tibial plateau...
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Báo cáo y học: "Suppression of nitric oxide production from nasal fibroblasts by metabolized clarithromycin in vitro" potx
Ngày tải lên :
11/08/2014, 03:20
... prevention of tissue injury in patients with chronic airway diseases, including CS The cellular response to LPS is transmitted from the cell membrane to the cytoplasm through the Toll-like Furuya ... [27] The present results clearly showed that CAM could exert the suppressive effect on the ability of NPFs to produce NO in response to LPS stimulation when the cells were treated with the agent ... 0.1 μg/ml of the agent was added to cell cultures On the other hand, the addition of M-4 at more than 0.04 μg/ml exerted the suppressive effect on NO production from NPFs (Figure 3B) The data in...
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Báo cáo y học: "Suppression of nitric oxide production from nasal fibroblasts by metabolized clarithromycin in vitro" pptx
Ngày tải lên :
11/08/2014, 06:22
... prevention of tissue injury in patients with chronic airway diseases, including CS The cellular response to LPS is transmitted from the cell membrane to the cytoplasm through the Toll-like Furuya ... [27] The present results clearly showed that CAM could exert the suppressive effect on the ability of NPFs to produce NO in response to LPS stimulation when the cells were treated with the agent ... 0.1 μg/ml of the agent was added to cell cultures On the other hand, the addition of M-4 at more than 0.04 μg/ml exerted the suppressive effect on NO production from NPFs (Figure 3B) The data in...
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Báo cáo y học: " Central role of nitric oxide in the pathogenesis of rheumatoid arthritis and systemic lupus erythematosus" potx
Ngày tải lên :
12/08/2014, 14:22
... in the cytosol, the mitochondrial membrane, and at the immunological synapse of T cells Localized NO production has been linked to targeting of endothelial NO synthase (eNOS) to the outer mitochondrial ... erythematosus T cells J Immunol 2008, 181:8145-8152 44 Moulton VR, Tsokos GC: Alternative splicing factor/splicing factor regulates the expression of the zeta subunit of the human T cell receptorassociated ... responsible for the anti-inflammatory effects of this medicinal plant NO treatment may lead to necrosis rather than apoptosis by decreasing intracellular ATP levels The release of intracellular antigens...
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Báo cáo y học: "Involvement of nitric oxide (NO) in cough reflex sensitivity between non-sensitized and OVA-sensitized guinea pigs" pptx
Ngày tải lên :
13/08/2014, 10:20
... of 60 strokes/min The expired air from the expiration valve of the ventilator was collected twice in the Mylar bag for 100 s Within h after sampling, the air was applied to the Sievers Model ... quarantined at the Animal Research Center of Kanazawa University All animal procedures in this study conformed to the standards set in the Guidelines for the Care and Use of Laboratory Animals at the Takara-machi ... the effect of NOS inhibitors in non-sensitized guinea pigs Vehicle (normal saline), the NOS inhibitor L-NAME solutions, or the iNOS inhibitor ONO1714 solutions were administered 0.5 h prior to...
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Báo cáo y học: "Time course of nitric oxide synthases, nitrosative stress, and poly(ADP ribosylation) in an ovine sepsis model" pot
Ngày tải lên :
13/08/2014, 21:20
... thereby further impairing pulmonary oxygenation There are some limitations of the study we want to acknowledge First, the study was designed to monitor the sepsis-related pulmonary tissue response ... increase early after the injury Albeit the changes in mRNA of eNOS and iNOS were not statistically significant, they tended to be elevated prior to the increase in protein expression of the respective ... artery catheter, a pulmonary artery thermodilution catheter, and a left atrial catheter After a recovery period of five to seven days, the animals received tracheostomy followed by inhalation injury...
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The role of nitric oxide and other gaseous mediators in cardiovascular disease models; emphasis on septic shock
Ngày tải lên :
14/09/2015, 14:16
... targets of therapy, particularly in the Lipid A component Lipid A has in fact been shown to be the portion of LPS which exerts the toxic effects and incites the inflammatory response of the host ... inciting mediator of the inflammatory response to these organisms in the host (see Chapter 1, Section 1.3.2.) and is composed of three primary components namely, the O (outer) polysaccharide, the core, ... sepsis likely due to both the heterogeneity of the clinical situations involved and the extreme complexity of the host response to overwhelming infection As such, the need to assess which animal...
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The role of nitric oxide and prostaglandin e2 in prolonged hemorrhagic shock 2
Ngày tải lên :
16/09/2015, 15:54
... for the physiological production of PGs On the other hand, COX-2 is an inducible isoform that is induced by cytokines, mitogens, and endotoxins in inflammatory cells and is responsible for the ... neuronal cells in the central nervous system (Szabo, 1996) PPP In recent years, the effect of iNOS inhibitors have been studied extensively in various injury and shock models due to their ability to ... free access to water They were anaesthetized with CRC cocktail (0.3 mL/l00 g body weight) intraperitoneally and were maintained under anaesthesia for the duration of the experiment The body temperature...
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The role of nitric oxide and prostaglandin e2 in prolonged hemorrhagic shock 1
Ngày tải lên :
16/09/2015, 15:54
... rats Immunohistochemical analysis showed a marked number of iNOS immunopositive cells in the cerebral cortex ipsilateral to the injury in saline-treated FPI+HS, FPI or HS rats Histological findings ... effect in the later stages of prolonged hemorrhagic shock The NOS inhibitor, AG, may be of interest as a therapeutic agent in attenuating organ damages when compared with the COX-2 inhibitor, NS-398 ... AG-treated rats when compared with the other treatment groups Histological examinations also showed a reduction of organ damages in AG-treated rats when compared with the other treatment groups Nitrate/nitrite...
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Role of nitric oxide in wound healing facilitatory effects of nitrosoglutathione a nitric oxide donor on the extracellular matrix deposition characteristics of wound healing
Ngày tải lên :
17/09/2015, 17:19
... growth factor, transforming growth factor, fibroblast growth factor-β, tumor necrosis factor and interleukin-1 The characterization of the effects of these factors and the ability to prepare them ... these two proteases digest basement membrane constituents The fragmentation of the basement membrane allows endothelial cells to migrate into the injured site As endothelial cells migrate into ... cutaneous injury, a time when myofibroblasts have filled the wound and are linked to each other and to the extracellular matrix This then may be the signal for wound contraction to commence (Lepisto,...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
Ngày tải lên :
31/10/2012, 15:12
... 24 hors for the cell line According to the PowerBlot data, increased expression of phosphorylated JNKs in JAR cells was de- tected after exposure to hypoxia On the other hand, JAR cells transfected ... also thought to be involved On the other hand, death receptor pathways are regulated by signals from death receptors that exist on the cell surface membrane Fas-induced apoptosis and/or the tumor ... changes in the proteins related to apoptosis during hypoxia to clarify the mechanism of hypoxia-induced apoptosis, and to find out useful hypoxia-related markers of trophoblastic cells Materials...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
Ngày tải lên :
14/02/2014, 22:20
... the available acyl-CoAs are in excess, they are channeled towards TAG synthesis The main findings of the present investigation were the observations that TAG synthesis in liver cells exposed to ... the appearance of toxic effects, supporting the notion that it constitutes the initiating event in the process of lipotoxicity Stearate has to be activated in order to be toxic The first enzyme ... leading to cellular dysfunction and ultimately to cell death The precise mechanisms lying beneath these processes remain elusive, and the key elements that determine the induction of toxicity...
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