the role of sialic acid in the formation of protective conformational bacterial polysaccharide epitopes
Báo cáo y học: "An anti-inflammatory role for tranexamic acid in cardiac surgery" docx
Ngày tải lên :
13/08/2014, 08:21
... how the fibrinolytic and pro-inflammatory responses are interlinked The interplay of other major pro- and antiinflammatory mediators is worthy of further study in this patient group (in particular, ... to the development of SIRS and a poorer outcome in this patient group The use of tranexamic acid in the study by Jimenez and colleagues [1] to attenuate inflammation reveals a glimpse of how the ... coagulation and fibrinolysis, has the potential to lead to more effective interventions for the clinician perioperatively and in the management of critically ill patients Competing interests The author...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
Ngày tải lên :
20/02/2014, 01:20
... role in maintaining SNase tertiary structure Our CD and DSC data show that the W140 in SNase is the amino acid responsible for the stability of the whole protein However, in comparison with the ... Summary Trp140 located in the C-terminal loop of SNase plays an important role in protein stability and conformational integrity During protein unfolding ⁄ refolding, the addition of 2.5 protons (both ... was removed, the secondary structure of the protein disintegrated Using the peak at 222 nm of the wild-type protein as an index of helical conformational stability, this CD spectrum of W140O suggests...
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
Ngày tải lên :
07/03/2014, 21:20
... near the flexible C terminus of SNase Changes in the fluorescence intensity of W140 reflect a change in the surrounding hydrophobic environment of the tryptophan and thus indicate a change in the ... key acidic amino acids in staphylococcal nuclease by the interactions of E75 with H121 and K97 It is believed that a small number of key amino acid segments play major roles in generating these ... decreases in fluorescence intensity, those amino acids around the tryptophan are probably still in the form of their native conformation This hypothesis is supported by the CD results, showing that...
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Báo cáo khoa học: 2-Oxo acid dehydrogenase complexes in redox regulation Role of the lipoate residues and thioredoxin pot
Ngày tải lên :
17/03/2014, 09:20
... catalytic role of these domains Rather, the advantage appears to depend on the ability of the lipoyl group to protrude from the inner core of the complex, indicative of the biological significance of the ... Thioredoxin catalysis of the dismutation of the thiyl radicals of the complex-bound dihydrolipoate intermediate Thioredoxin protection from the 2-oxo acid, CoAinduced inactivation of the dehydrogenase ... the effect of thioredoxin on the activity of the complex This relationship suggests coevolution of the interacting proteins, which would not be possible if the interactions were not relevant in...
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Báo cáo Y học: Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase A site-directed mutagenesis study docx
Ngày tải lên :
17/03/2014, 17:20
... in pkDAAO and Y238 in RgDAAO share the characteristics of being exible and adapting their conformation depending on the size of the ligand side chain [27] Our results indicate that the role of ... following the binding of anthranilate (Fig 3, inset) and the shoulder at % 500 nm following the binding of benzoate (De497nm of 7500ặM)1ặcm)1 vs a gure of 2000 4000ặM)1ặcm)1 observed with the other ... in binding were observed for Y238 mutants with the ligands tested (Table 2) These results indicate that the mode of ligand binding is retained in the two mutants, and that the alteration of the...
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Báo cáo khoa học: The role of cytochrome P450 monooxygenases in microbial fatty acid metabolism pdf
Ngày tải lên :
22/03/2014, 16:21
... strong indications for the involvement of a bacterial P450 in the hydroxylation of octane in Rhodococcus rhodochrous, the role of bacterial P450s in alkane degradation in addition to the well-established ... tight binding affinity was demonstrated towards lauric acid, resulting in the synthesis of mainly x-1 hydroxylated 216 lauric acid, suggesting a role in the lipid oxidative metabolism Interestingly, ... occurring in the soil Furthermore, branched chain fatty acids make up 80% of the fatty acid content of the Bacillus sp membranes and when the hydroxylation of these substrates was studied in more...
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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx
Ngày tải lên :
30/03/2014, 11:20
... produced when coat protein is overexpressed in E coli from a plasmid [11] To examine the stability of the coat protein dimer in the absence of the intersubunit interactions found in the capsid, we used ... of the protein emit fluorescence when excited at 280 nm When the protein unfolds in the presence of denaturing agents, the exposure of buried residues reflects the conformational changes in the ... and whether it is related to the normal RNA-binding function of coat protein, as RNA binding had no effect The increased light scattering of samples after pressure release in the presence of bis-ANS...
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Báo cáo Y học: Probing the role of glutamic acid 139 of Anabaena ferredoxin-NADP+ reductase in the interaction with substrates pptx
Ngày tải lên :
31/03/2014, 08:20
... differences in the microenvironment of the isoalloxazine ring [21,22] Moreover, the structure of E301A FNR also showed interesting conformational changes in the side-chain of another glutamic acid residue, ... FNR:Fld interaction In summary, our results indicate that E139 is not involved in the processes of binding and ET between FNR and NADP+/H, while the nature of the charge and the conformation of the ... suggested that in E301A FNR the side-chain of E139 might in uence the properties of the flavin, assuming some of the functions carried out by E301 in the wild-type enzyme [22] In this context,...
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The role of carbon in valve-regulated lead–acid battery technology potx
Ngày tải lên :
05/07/2014, 20:21
... however, the importance of negative-plate characteristics (particularly the stability of the electrode’s potential during charging) in determining the suitability of VRLA batteries for a range of other ... improvement in performance of the carbon|lead acid HES device, due to the incorporation of lead into the carbon electrode, clearly makes it a stronger development proposition The presence of lead in the ... Roughly 60% of the carbon black was consumed during formation and the remainder disappeared early in cycling Interestingly, this carbon black significantly increased the ␣/-PbO2 ratio and the total...
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Báo cáo y học: " Role of taurine on acid secretion in the rat stomach" ppsx
Ngày tải lên :
10/08/2014, 05:21
... indicated by CSAD- and taurine-positive cells We found the presence of taurine-containing cells and taurine-induced acid secretion in the stomach In the body of the stomach, taurine-immunoreactive cells ... various doses of taurine and acid secretion Values are the mean ± SEM (n = 6) increases the level of cAMP Fourth, the presence of taurine-containing cells in the rat stomach is confirmed, as indicated ... during oxidation Taurine-containing cells are present in the myenteric plexus and submucosal plexus of the enteric nervous system in the stomach Taurinergic neurons in the muscle layer of the...
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Báo cáo y học: "Possible role of alpha-lipoic acid in the treatment of peripheral nerve injuries" ppsx
Ngày tải lên :
10/08/2014, 10:20
... suggested the importance of a-LA in the treatment of peripheral nerve injuries, but further studies are needed to explain the mechanisms of its neuroprotective effects and before the use of a-LA in ... may be a response against oxidative stress The results of Senoglu and colleagues correlated with our recent findings in which the combined employment of a-LA and g-linolenic acid with a rehabilitation ... nerve injury [1] The activities of SOD and CAT were found to be high in sciatic tissue of rats indicating high production of superoxide anion radical Therefore, the increase of SOD and CAT activities...
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Báo cáo y học: " Role of lysophosphatidic acid receptor LPA2 in the development of allergic airway inflammation in a murine model of asthma" potx
Ngày tải lên :
12/08/2014, 14:20
... cytokines play a key role in orchestrating inflammatory and structural changes of the airway in asthma by recruiting, activating and promoting inflammatory cells into the airway [38-40] In addition ... acute lung injury [27] These data suggest a protective role of administrated LPA in airway inflammatory diseases In contrast to several in vitro studies on the role of LPA as a pro- or anti-inflammatory ... suggesting an antiinflammatory role of LPA in a murine model of acute lung injury [26] In addition to its anti-inflammatory effect, LPA regulated E-cadherin intracellular trafficking and airway epithelial...
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Báo cáo khoa học: "Bench-to-bedside review: Treating acid–base abnormalities in the intensive care unit – the role of renal replacement therapy" potx
Ngày tải lên :
12/08/2014, 20:20
... on acid base disorders Therefore, improving our understanding of the impact of RRT on acid base disorders and gaining insights into the nature of such disorders and the mechanisms of action of ... the effect of HVHF on acid base balance A strong focus is given to the clinical implications of these interventions, with the aim of helping clinicians better understand and manage the acid base ... hypoalbuminemia Hypoalbuminemia lowered the anion gap and masked the presence of acidifying anions to those clinicians using conventional acid base analysis Effect of renal replacement therapy on acid base...
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Báo cáo khoa học: "Bench-to-bedside review: Treating acid–base abnormalities in the intensive care unit – the role of buffers" ppt
Ngày tải lên :
12/08/2014, 20:20
... carbonic acid, it is misleading to assume that [HCO3–] is an independent determinant of [H+] In fact, the independent determinants of [H+] in the blood are the strong ion difference [SID], the total ... different acid base consequences For example, infusing large volumes of normal saline intravenously lowers the [SID] (because the [SID] of saline is zero), raising [H+] (and lowering pH) Whether the ... examining the effects of bicarbonate infusion on intracellular pH are variable, with intracellular [H+] rising [36], falling [37,38,43–48], not changing [4,14,34,35], or either rising or falling...
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THE ROLE OF ACID SPHINGOMYELINASE IN AUTOPHAGY
Ngày tải lên :
24/08/2014, 09:45
... The resident location of mTOR in the cell is in the lysosomal nutrient sensing complex which is docked at the membrane of the lysosome [14] If either availability of amino acids is limited or mTOR ... were stained for phosphatidylserine using Annexin V staining (Annexin V Kit 250 Samples; VWR), following manufacturer’s protocol of washing cells in unicellular suspension with PBS containing calcium, ... choloroquine to not exacerbate the accumulation of LC3B-II or p62 On the contrary, if imipramine does not inhibit the flux, administration of chloroquine, which blocks the flux, will further increase...
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The role of CDC42, 1RSP53 and its binding partners in filopodia formation
Ngày tải lên :
14/09/2015, 14:14
... G-actin and F-actin forms The rate of cycling is determined by actin binding proteins (ABPs) which includes the sequestering proteins and the capping proteins Sequestering proteins inhibit polymerization ... Profilin and ADF cofilin bind G- and F-actin and they are mostly concentrated at the leading edge of the cell They promote the disassembly of actin filament Gelsolin is responsible for F-actin ... branching of F-actin with angle of 70o 15 Figure 1.3 Actin Network Cargo Cargo Cargo Key: F-actin Profilin Myosin X Arp2/3 ADF Cofilin Gelsolin Myosin I Filamin Adhesion receptors α-Actinin Fimbrin...
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phosphatidylserine exposure in red blood cells: a suggestion for the active role of red blood cells in blood clot formation
Ngày tải lên :
15/11/2015, 12:54
... Methods F Investigation of the kinetics of PS exposure To investigate the kinetics of the PS exposure processes, the washed RBCs were put on a coverslip in physiological solution in the presence of ... important role in activating scramblases, thereby leading to the exposure of PS to outer leaflet of the cell membrane The activation of Ca2+-activated K+ channel (Gardos channel) by an increase of intracellular ... to the manual instruction, the Kd for the binding of annexin V to PS has been estimated at 5.10-10 M The annexin V binding 32 Materials and Methods assay is based on the rapid and selective binding...
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The implication of microglial sialic acid binding immunoglobulin like lectin e (siglec e) in neuroinflammation
Ngày tải lên :
19/11/2015, 15:52
... mediates sialic acid binding The preference for a certain type of sialic acid is determined by a sequence of six amino acids in the C-C´ loop of the V-set domain (Yamaji et al 2002) The V-set ... domain-containing protein tyrosine phosphatases (SHP)-1 and SHP-2 They are recruited via the binding of their SH2 domain(s) to the phosphorylated ITIM The activated SHP1 then dephosphorylates intracellular ... alpha2-8 linked sialic acid preferentially to alpha2-3 and alpha2-6linked sialic acid (Zhang et al 2004) The amino acids, which are indispensable for binding of sialic acid, are conserved in Siglec-E...
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Circuit theory of finance and the role of incentives in financial sector reform
Ngày tải lên :
24/10/2012, 09:33
... profitability is declining The internalization of information within the same institution may thus enhance intra-circuit and inter-circuit stability by reducing the incentive to overfinancing The ... Creating a stronger investor base amounts to strengthening the circuit by completing the financial market structure of the economy, thus eliminating discontinuities in the transmission of information ... interpreting and judging are parts of the production process of information; disseminating information to the general public through reports or advertising campaigns, and providing individualized information...
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Facts of Vietnam Freight forwarding industry and the role of Vietnam Freight Forwarders Association (VIFFAS) to the industry in international economic integration processhelpful to Oristar.doc
Ngày tải lên :
27/10/2012, 16:42
... advantageous in serving transport in the Mekong Delta linking to Cambodia The total length of the inland waterway (IW) system is about 40,998 km, mainly in the Red River Delta and the Mekong Delta In the ... forwarding activities of the S.R of Vietnam, and on this basis to integrate with such professional colleagues on the world The Association has the role of coordinating the activities of the members ... conditions for business associations, as well implemented strategies on developing and increasing the quality of trade services of the Ministry of Trade and the Ministry of Transport Being the intermediary...
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