... lineages of mitochondrial DNA exist among the San (‘Bushmen’) of southern Africa and the Biaka Pygmies ofthe modern Central African Republic. About 100,000 yearsago, some of these anatomically ... enteredMediterranean trade. Their raids extended at least as far southwards as LakeChad. The Garamantian state peaked during the second and third centuries adwhen the Roman colonies were also most ... truly historical sense emerged; learningconsisted ofa gigantic catalogue of names and attributes; and the law was notcodified. The state was a mass of individual of cials, tasks, and institutions;unlike...
... said: the great masses of humanity are using the Law destructively, or partially so, and the scales are balanced against them. Here and there, among the masses, we find an occasional outstanding ... as the beasts ofthe field, the birds ofthe air and the fish ofthe sea are bountifully supplied. For any man, no matter what his station in life, to take the stand that it is the destiny of ... that I had met my deliverer, and at the close ofthe performance was overjoyed at his invitation to accompany him to a nearby café. I noticed that the attention of those in the café was drawn...
... satisfactionwith their work. The results are valued on a Likert scale of 1to 7 for the different items. Over the last few years, the averagesatisfaction index—calculated as the average ofthe values givento ... the participation of all the members ofthe organiza-tion.* Implementation ofa KM strategy is developedthrough several phases. The process starts withdissemination ofthe ideas to all ofthe organiza-tion ... factors in the success of the process and the advances made in KM in relationto innovation. These include the main factors in the firm’s strategic change that the implementation of such a strategy...
... functionality ofthe ADO Recordset's GetRows( ) method. The sample code contains an event handler and a method: Go Button.Click Sets up the sample by creating a DataTable containing the Orders table ... the rows and columns in the table. The number of columns in the array is set to hold either all ofthe columns in the table or the subset of columns defined by the optional string array of column ... = Math.Min(nRows, rowCount); // Create an object array to hold the data in the table. Array a = Array.CreateInstance(typeof(object), nRows, nCols); // Iterate over the collection of...
... because ofthe absence ofthe catalyticsubunit ISP (Table 1). Figure 3A shows that a band of approximately 500 kDa was also found in this mutantstrain when the mitochondrial membranes were ana-lyzed ... dimerization ofthe bc1complex atthis stage (i.e. just after the addition of ISP and Qcr10p).On the other hand, in the transition from the 500 kDaband to the 670 kDa band, a structural rearrangement of ... were of analytical grade.Yeast strains and growth media The genotypes and sources ofthe S. cerevisiae strains aredescribed in Table 2. The ISP deletion strain wasprepared in accordance with the...
... Institute of BioAgricultural Sciences, Academia Sinica, Taipei, Taiwan, ROC2 Institute of Physics, Academia Sinica, Taipei, Taiwan, ROC3 Department of Biochemistry, University of Minnesota College of ... structure-based analysis of staphylococcal nuclease. Proteins: Structure, Functionand Genetics 27, 171–183.15 Flanagan JM, Kataoka M, Fujisawa T & EngelmanDM (1993) Mutations can cause large changes ... reportedthat multiple mutations can cause large changes in the average conformation of denatured proteins. Here weshow that a specific single mutation or removal of a specific fragment can cause large...
... against any of the transgalactooligosaccharides listed in Materials andmethods.Hydrolysis of arabinogalactans The sugar composition ofthe arabinogalactans frompotato, onion and soy was determined ... soy arabinogalactan consists of 57%D-galactose and 38%L-arabinose. Methylation analysisdemonstrated that a substantial amount of the L-arabinoseresidues (14%) in soy arabinogalactan is ... of GALA with different arabino-galactans was measured in duplicate by HPAEC andMALDI-TOF MS after incubation of 1 mgặmL)1 of potato, onion and soy arabinogalactan with 1.33 lgặmL)1GALA at...
... Peplow, F. Yamakura and T. Matsumoto for the analyses of iron and manganese in protein samples. We also wish tothank H. Steinman for the gift of E. coli OX32 6A. We finally thank MrM. Farrugia for ... helper phage (Stratagene). One microgram wasutilized in mutagenesis reactions together with oligonucleo-tides ECF-Q69G d(5Â-AACAACGCAGCTGGGCTCTGGAACCAT), ECF -A1 41Q d(5Â-TCAACCTCTAACCAGGCTACTCCGCTG) ... d(5Â-AACAACGCTGGCCAGCACGCTAACCAC) and ECM-Q14 6A d(5Â-TCTACTGCTAACGCGGATTCTCCGCTG) following the manufacturers instructions (mutagenic nucleotide areunderlined). Mutated plasmids were designated...
... higher value almost 2.5-fold higher than the native cold adapted enzyme (Table 1). The mutantG26 1A/ Y26 9A exhibits an E a almost the same as in the caseofthenativeenzyme(Table1).Thermal inactivation ... gene encoding alkaline phosphatasefrom the Antarctic strain TAB5 [16]. Based on the crystalstructure (at 2.4 A ˚)ofanEscherichia coli alkaline phospha-tase variant with a 28% amino-acid sequence ... from the AntarcticTable 1. Enzymatic and thermodynamic parameters ofthe psychrophilic alkaline phosphatase and mutants. Reported values were determined at10 °C. The kcatvalues were calculated...
... b-strand 3 and a- helix 3 ofthe barrel, and a C-terminal, domain C, folded as an antiparallel b-sheet[17–23]. Acarbose is a pseudotetrasaccharide inhibitor of a- amylase, that acts like a transition-state ... fromSigma. Maltodextrin of average DP18 (2.9 kDa) wasfrom Hayashibara Biochemical Laboratories (Okayama,Japan). Reduction ofthe DP18-maltodextrin to the corresponding alcohol was performed as earlier ... lMdecreasing to 37.3 lMby addition of acar-bose. A 0is the AMY1 absorbance without acarbose, A is the absorbance measured at the above acarbose concentrations.(B) Reciprocal plot ofthe difference...
... 2002 Academia Sinica (Taipei, Taiwan) for assistance in the chemicalsynthesis of inhibitor analogues. We thank Dr Yuch-Cheng Jean of the Synchrotron Radiation Research Center (Hsinchu, Taiwan) and ... 13129–13137.12. Yamakawa, Y. & Omori-Satoh, T. (1992) Primary structure of the antihemorrhagic factor in serum ofthe Japanese Habu: a snakevenom metalloproteinase inhibitor with a double-headed cystatindomain. ... shchiou@gate.sinica.edu.twand A. H J. Wang, Institute of Biological Chemistry, AcademiaSinica, Nankang, Taipei, Taiwan.Fax: +886 227882043, E-mail: ahjwang@gate.sinica.edu.twAbbreviations:...
... till their hands and legs swell up and they can hardly walk? What about the tears and pain of their mothers? Or are they all expendable as long as you get that cool gadget on time?This is how the ... a Dictator A Short and Snappy GuideBecome an Atheist A Short and Snappy GuideMind Candies for a Lousy Day A Short and Snappy GuideGet to the Point! A Short and Snappy GuideHe can be contacted ... international companies take advantage of that. They don’t go to third world countries to improve diplomatic and cultural relations; they go there because there are no proper labor rules and...
... that I prepared for my new home; and what added to my unhappiness, was the fact that my brother William was purchased by the same family. My father, by his nature, as well as by the habit of ... She was sold to Georgia. brought a charge of theft against any of his slaves, he was browbeaten by the master, who assured him that his slaves had enough of every thing at home, and had no ... Flint handed them both over to a slave-trader. The guilty man put their value into his pocket, and had the satisfaction of knowing that they were out of sight and hearing. When the mother was delivered...