... Particulate Matter Under the Clean Air Act: A MenuofOptions (PM2.5 Menuof Options) to assist state and local air pollution control of cials in evaluating theoptionsfor reducing fine particulate ... of their contribution to the overall emissions profile of these facilities Cement Manufacturing The largest source of emissions in cement manufacturing— and the centerpiece ofthe process—is the ... use the results of site-specific source testing as the basis of their emissions inventory Much ofthe emissions inventory is based upon the application of emissions factors The development of emissions...
... analysis (A) Northern blot analysis Each lane contains 15 lg total RNA The bottom panel shows the expression of 18S rRNA as an internal control Note that the blot was exposed to the film forthe longest ... heme-independent transcriptional regulation ofthe HO-1 gene These metabolic consequences of HO-2 knockdown suggest a regulatory role for HO-2 in the co-ordinated expression of HO-1 and HO-2 We therefore propose ... by knockdown of HO-1 or HO-2 expression To explore the biological implication ofthe knockdown experiments and to evaluate the relative contribution of HO-1 and HO-2 to the total amount of heme...
... display the VR environment [9] The subjects were asked to place their hands on the table with the palms facing downwards, and faced the monitor at a distance of approximately 70 cm The image on the ... than lasers, they have several advantages: they can be applied directly on the body surface without need for lenses or fibers and they are inexpensive Furthermore, they are harmless forthe eye, ... analysis, and drafted the manuscript TM and FS carried out the MATLAB® pre-processing KE and DK participated in the design ofthe study MW participated in the design and coordination ofthe study All...
... applyed for all graphs calculations Comparisons ofthe distributions of three TM4SF members expression for different groups were performed using the Wilcoxon-Mann-Whitney test (for two groups) or the ... understanding ofthe potential role of TM4SF in metastasis, we can go on to collect information of follow-up, and analyze the relationship of CD9, CD63 and CD82 with the Days after surgery And in the future, ... be of tremendous interest to the drug discovery industry forthe development of therapeutics agents It is conceivable that investigates the tumors for TM4SF expression, in combination with other...
... the control group (d23c) Equimolar amounts ofthe mouse cDNAs from each group were pooled together and were analyzed in triplicates Forthe human samples, Ct values of both the GOI-ING4 and the ... 5(C) respectively Discussion In the present study we analyzed, forthe first time in the literature, the expression profiles of ING4 in the experimental model of pulmonary fibrosis as well as ... series of experiments and to shed further light on the fibrogenic cascade, we sought to determine the expression profiles of ING4, also known as inhibitor of HIF-1a, in different forms of pulmonary...
... design ofthe study, the acquisition, the analysis and the interpretation ofthe data, the statistical analysis and drafted the manuscript GP participated in the conception and design ofthe study ... targeting the evaluation ofthe clinical use of KLK5 expression forthe discrimination ofthe malignant from the non-cancerous tumors ofthe mammary gland Significantly decreased KLK5 transcription ... line as the calibrator ofthe assay However, the use of 2-ΔΔCT method requires the approximately equal amplification efficiencies ofthe target and the reference genes Therefore, prior to the assessment...
... tolerance to the sedative effects of diphenhydramine developed after a few days of scheduled treatment as has been suggested by other authors [2] During the course ofthe first 28 weeks of therapy ... based upon the severity of her symptoms and previous successful outcomes [5] After the first treatment with omalizumab, the patient noted significant improvement Over the course ofthe subsequent ... five fold greater expression of FcεRI prior to treatment with omalizumab After the first 14 days of treatment, there was an approximate 80% decrease in the expression ofthe high affinity IgE receptor...
... drawback using of bulk production assays, such as ELISA, forthe determination of cytokines in supernatants is the fact that they measure accumulated proteins over the incubation time They become ... and sTNF-Rs, respectively Therefore, the purpose of this study was to monitor the capability of peripheral blood monocytes of patients with multiple injuries during the early posttraumatic course ... principally given as the standard error ofthe mean Data are plotted as boxes (interquartile range that containsthe 50% of values) with a straight line at the median, a dashed line at the mean, and...
... with other transcription factors In such non-classical model, ER does not bind directly to the promoter ofthe regulated gene, but participates in the transcription ofthe gene by forming the pre-initiation ... thank for your constructive comments during all the meeting sessions I am also grateful forthe opportunity Prof Liu offered to let me some ofthe crucial experiments in his lab and for all the ... influence the transcriptional activity of ER, characterization of histone and DNA modification of ER specific genes, and finally the identification ofthe cis-regulatory ER-binding sites within the...
... (Figure 13B) To further determine the effects of 15d-PGJ2 on the growth of breast cancer cells lines, the cells were challenged with increasing doses of 15d-PGJ2 for 24h, before they were stained ... significant down- regulation of NHE1 To further verify that the reduction in NHE1 protein level was a result of transcriptional repression of NHE1 gene, we quantified the mRNA expression of NHE1 using ... doses of 15d-PGJ2 Overexpression of PPARγ after 48h of transfection is shown at top panel After 16h of drug treatment, the mRNA expression of NHE1 was analyzed by real-time PCR, normalized to the...
... charcoal/dextran treated serum for another 24h, before they were exposed to increasing doses of 15d-PGJ2 for 3h The activity of ERα was then determined using luciferase assay and the result was calculated ... failed to rescue the down- regulation of NHE1 mRNA by active PPARγ in this cell line On the other hand, the same 3μM of 15d-PGJ2 only decreased the NHE1 mRNA to about 75% ofthe control in MDA-MB-231 ... MDA-MB-231 cells in the absence of E2 However, when these cells were pre-incubated with 1μM of E2 for 2h, the down- regualtion of NHE1 by PPARγ ligand was much attenuated (Figure 19B) To further confirm...
... high doses of fulvestrant, and observed the extent of NHE1 down- regulation by PPARγ ligand In both case, removal of ERα restored the ability of 15d-PGJ2 to repress the expression of NHE1 both ... However, other than the ER status, the sensitivity of these cells to PPARγ ligands could be confounded by the different basal levels of PPARγ receptor present To confirm the involvement of ERα in ... M., V Muller, et al (1995) "The carboxy-terminal F domain ofthe human estrogen receptor: role in the transcriptional activity ofthe receptor and the effectiveness of antiestrogens as estrogen...
... the morphology ofthe cells (B) MCF-7 (3 X105 cells/6-well dishes) cells were treated with ciglitazone for 24h before pictures were taken to show the morphology ofthe cells (C) ... level of PPARγ showed the least sensitivity to 15d-PGJ2, while 15d-PGJ2 resulted in the maximal reduction in the number of colonies formed by MDA-MB-231 cells expressing the highest level of PPARγ ... with GW9662 before exposure to 15d-PGJ2 As shown in the long-term conloy forming assay, GW9662 protected the MCF-7 cells from the significant reduction in the number of colonies formed after treatment...
... confirm whether the inhibitory effect of ERα on PPARγ-mediated reduction of NHE1 expression involves the transcriptional activity of estrogen receptor 3B3.1 ERα antagonist enhances the PPARγ-mediated ... successful inhibited the transcriptional activity of ERα Moreover, the transcriptional activity of ERα was assessed using ERE luciferase reporter assay Luciferase reporter assay was then performed on MCF-7 ... NHE1 down- regulation, the question remains if this inhibitory effect of E2 extends to functional transcription of other bona fide target genes of PPARγ Such PPARγ effecctor genes responsible for...
... system, in the distal segment ofthe tubule Inhibition of glucose reabsorption in the kidney, mediated by the SGLT cotransport system, represents a promising therapeutic target forthe control of hyperglycemia ... larvae of beetles (Chrysomelidae) Their synthesis has been previously described [31-33] Forthe purpose ofthe present study the thioglycosides used were selected and grouped based on their differences ... IC50 values of 30 µM for hSGLT1 and 10 µM for hSGLT2, while thioglycoside VII showed IC50 values of 15 µM for hSGLT1 and 88 µM for hSGLT2 These data confirm the strong inhibitory effects of thioglycoside...
... a quick and effective tool forthe control of stored-product insect pests In view ofthe scheduled phaseout of methyl bromide under the Montreal protocol, the role of phosphine in grain protection ... 2002) ITCs were chosen for this study because ofthe pesticidal properties of these chemicals (Fenwick at al., 1983) and because ofthe potential use of methyl ITC as fumigant for wheat (Ducom, 1994) ... used The main component of one ofthe oils was pulegone and ofthe other is not yet totally identified, and it is called SEM76 In space fumigation, these two volatiles caused total mortality of...
... enhance the author to this thesis Then, the purpose ofthe study introduces the aims to the thesis And the scope ofthe study gives the details about the objects that the thesis studies and the main ... writing Most ofthe learners of this program are adults Especially, at the first level, the mojority ofthe learners are at the age from nineteen to forty Therefore, the author ofthe thesis would ... following part ofthe thesis 3.2.3.2 The results ofthe study i The results ofthe questionnaires First of all, the author would like to show the chart ofthe learners’ purposes to take part in the GEP...
... Navigator TheScriptpane toolbar appears above theScriptpane and provides a series of buttons and commands, enabling you to add to or edit the current script in theScriptpane in various ways In the ... a new ActionScript file, ready forediting in the ActionScript editor The ActionScript editor looks very similar to how it looked in the previous steps The most noticeable difference is the fact ... script attached to it in theScript pane, allowing you to navigate quickly through the scripts in your project forediting purposes Only elements with scripts attached to them appear in the Script...
... addition to these forecasts ofthe primary population, there are similar projections ofthe number of dependents who will be eligible for benefits (survivors’ benefits for Because ofthe practice of allowing ... 10 The old formula used the pay in the last month before retirement as the benchmark, and the current formula, called High-3, takes the average ofthe highest paid three years of service as the ... AEAN formula In principle, the number of years used in the denominator is a matter of choice; for instance, the payments forthe projected benefits ofthe entry cohort can be taken in a series of...