... carrying the masts of their boats. When they reached theoldman s shack the boy took the rolls of line in the basket andthe harpoon and gaff andtheoldman carried the mast with the furled ... Hemingway TheOldManandtheSea 22 right foot on the fish’s head. Then he turned him over and skinned the other side and cut each side off from the head down to the tail. [78] He slid the carcass ... walked up the road together to theoldman s shack and went in through its open door. The oldman leaned the mast with its wrapped sail against the wall andthe boy put the box andthe other gear...
... asleep. Landing the fish didnot matter to theoldman only to get it as far as the side of the boat. The OldManAndTheSea portrays Hemingway Code Heros totheir fullest potential. As Heros they ... hisprey implicates the importance of his relationship with it. Santiago'shumility in TheOldManAndTheSea should be an example for all tofollow. He fishes to be a fisherman. His goal was ... other the best of company. While out at sea Santiago isconstantlywishing the boy was there to talk to or to help with the mightyfish. Santiago does not have relationships with any of the otherfishermen,...
... cutter and two other craft and almost blew them, much larger though they were, out of the water. Was this the beginning of the war, the Opium War? In those days, formality and habit required at the ... file:///C|/Documents %2 0and% 20 Settings/Owner/My %20 Docume ary.com/nlreader.dll@bookid=4 128 1&filename=page_13.html [9/13 /20 07 3:15 :22 PM] DocumentPage 9surface of the pipe bowl, tipped the bowl over the flame, ... Bridgman at the Repository; like Bridgman he observes and reports the opium crisis andthe war. file:///C|/Documents %2 0and% 20 Settings/Owner/My %20 Docume eader/nlreader.dll@bookid=4 128 1&filename=page_xxxi.html...
... answered the child, ‘for father and mother to eat out of when I am big.’ The manand his wife looked at each other for a while, and presently began to cry. Then they took theold grandfather to the ... is my husband!’ she quickly hid the roast meat inside the tiled stove, the wine under the pillow, the salad on the bed, the cakes under it, andthe parson in the closet on the porch. Then she ... calf, and paint it brown, so that it looks like any other, and in time it will certainly get big and be a cow.’ the woman also liked the idea, and their gossip the carpenter cut and planed the...
... point, however, the potato entered a turbulent sea of slander and semantics. The slander came at the hands of the Swiss botanist Caspar Bauhin, who wrote in the last years of the sixteenth century ... 135CHAPTER14 THE COLUMBIAN EXCHANGE ANDTHE OLD WORLDS And the trees are as different from ours as day from night; and also the fruits, and grasses and stones and everything. Christopher ... Columbian Exchange andtheOld Worlds 137ones? And, for that matter, why should they eat foods to which they were unaccustomed and that demanded new preparation methods? Weaning the peasantry...
... your original demands. The basketball player is holding out for a million dollars a game. The union spokesman said the union members would hold out until their demands were met. holdout n. When ... hand, but he didn't shake it. The street was filled with beggars holding their hands out. 2. hold out p.v. When a supply of something is enough for your needs, the supply holds out. The ... using the objects in parentheses. Be sure to put the objects in the right place. 1. They burned up. (the wood, it) 2. He ought to clear up. (the misunderstanding, it) 3. Would you count up? (the...
... (kJặmol)1)DHcorra(kJặmol)1)TDS(kJặmol)1)TDScorra(kJặmol)1)NarJNarG( 128 ) 0 .2 ± 0.1 2. 3 ± 4 · 10)9)69.4 ± 3.8 )22 )20 .1 27 .30.9 ± 0.1 1.7 ± 3.1 · 10)7) 62. 1 ± 3.1 )14.7 )23 .4 24 NarJT–NarG(1 28 ) 0.3 ± 0.1 7.3 ± 3.8 · 10)9)57 ± 2. 7 )9.6 )10.6 ... MgCl 2 , 100 mM NaCl. The upper panels show the raw data for the heat effect during the titrations; the lowerpanels are the binding isotherms.Table 2. Thermodynamic parameters of NarG(1–15) and ... antiparallel pair of b-strands(residues 16–19 and 22 25 ). The N-terminal helix wassimilar to that observed in the NarG X-ray structure(rmsd = 2. 84 A˚for the backbone) [4]. However, the orientation...
... infosfomycin binding: K 22 and R 120 . To investigate the roleof these residues, we have generated the K22V, K22E, K22R and R 120 K single mutant proteins as well as the K22V/R 120 K and K22V/R 120 V double mutant ... MurA K22V MurA R 120 K MurA K22V/R 120 K MurADH )46.8 )44.0 )45.9 )33.8DG )25 .5 )23 .0 )23 .0 )22 .8TDS )21 .4 )21 .0 )22 .9 )11.0Kd(lM) 28 .4 80.5 80.0 87.6DCp)1.9 )2. 0 )1.4 )1.38Ó FEBS 20 04 ... +UDPNAGActivity(%)Wild-type + + 100K22R + + 0.3K22V – + 0.03K22E – – 0.05R 120 K – – <0.05K22V/R 120 K – – *K22V/R 120 V – – *Fig. 3. Incubation of the K22V/R 120 K double mutant protein withfosfomycin...
... 1 August 20 03, revised 2 October 20 03,accepted 7 October 20 03)Eur. J. Biochem. 27 0, 4 729 –4735 (20 03) Ó FEBS 20 03 doi:10.1046/j.14 32- 1033 .20 03.038 72. x systematic mutagenic approach, the role ... reference the heat capacity of the native state[15].ResultsRecombinant mBS and its P19A, L28Q and P19A/L28Qvariants (P19A-mBS, L28Q-mBS and P19A/L28Q-mBS,respectively), all with Cys31 and 32 linked ... For the mutant P19A/L28Q-mBS the mutagenic primer L28Q was used starting from the pET -22 b(+) plasmid cDNA coding for the mutant P19A-mBS. The amplified, mutated genes were separated, excised,and...
... obtainedbyahyperplanecut.Then,V(E,G)ρm(E,G)n≥πn /2 2Γ(n /2+ 1)+π(n−1) /2 Γ(n −1) /2+ 1, (2. 5)where the equality is attained, for instance, for the optimal set described in Figure 2. 2. 6 Comparing ... So,V(E,C)ρm(E,C)n≥V(E,C)ρm(E,C)n=πn /2 2Γ(n /2+ 1)+π(n−1) /2 Γ(n −1) /2+ 1. (2. 7) The example in Figure 2.2 shows that this is the best possible lower bound. (2) Suppose that ρm(E,G) =ρ(E)andV(E,G) =V(G \E).In this case we can translate the ... comparing the relative perimeter and the minimum relative diameter (the minimum between the diameter of E andthe diameter ofits complement) for subdivisions of planar convex sets (see [2] ), and the...