... in the one-cell stage to visualize the localization and to measure the mobility ofthe components in the PGCs The medaka embryo was peeled off the chorion, and the segment containing the part of ... of OlvasGFP reveals the dynamic nature ofthe nuage Schematic diagram ofthe preparation of PGCs of medaka specimen (A) OlvasGFP was expressed in the medaka PGC at stage 24 and localized in the ... outside ofthe nuage in the cytoplasm ofthe migrating PGC FCS analysis revealed that Olvas GFP diffused with a diffusion constant of 7.0 lm2ặs)1 (Fig 3A) During the measurement ofthe PGC, the 10...
... (pTDN1) [14] The lack of characterization ofthe structural determinant ofthe substrate specificity ofthe AtdA enzyme has thus limited its development as a biocatalyst for the bioremediation of a wide ... reported which ofthe five genes control the substrate specificity ofthe AtdA enzyme The objective of this study was to identify and probe the residues determining the activity as well as the substrate ... may indicate that the steric limitation ofthe enzyme’s binding pocket takes place in the area between the ortho and para positions ofthe aromatic substrate On the basis of these results, aniline...
... wild-type ALP I On the other hand, the Tm of D266N/Y269A-ALP I increased by only °C The denaturation temperature indicates the stability ofthe structure ofthe protein The difference ofthe increments ... surrounding residues ofthe depression, and the side chain ofthe mutated residue might restrain the mobility ofthe N-terminal region on the surface by interaction with the main chain of Lys26 by van ... the substitutions did not influence the activities or specificity ofthe mutant enzymes The N-terminal region of ALP I was also on the surface The side chains of Ile10 and Tyr11 were close to the...
... Tyr170-Ca, and the side chain of Tyr170 is rotated Through the combined effect, ˚ Tyr170-Og moves a total of approximately 11 A, resulting in the formation of hydrogen bonds with the nitrogen ofthe amide ... simplify the estimation ofthe specific activity of each enzyme, we quantified the amount of Hyb-24-related protein and Ald-hydrolytic activity in cell extracts, and normalized the data on the basis of ... binding at the C-terminal region of Ald is improved by the D370Y substitution The roles of Asn266 and Tyr370 were further examined by site-directed mutagenesis Roles of Asn266 Superimposition of Hyb-24DNY...
... the location of amyloidogenic mutations and position of b-strands (A) The structure ofthe polypeptide backbone ofthe two chains (purple and blue) is shown along with the location ofthe N-terminus ... tissue and that the distribution of TTR deposition reflects the presence of these endogenous factors In the case ofthe Ab protein of Alzheimer’s disease, a number of proteins and factors (pathological ... these diseases, in particular Alzheimer’s disease, may help to explain some aspects ofthe pathogenesis of FAP The idea is discussed further in the following sections Tissue-specific pattern of...
... itself both at the level ofthe immediate chromatin environment ofthe intergenic region, and also at the level of topological status ofthe DNA as a result of transcription ofthe adjacent genes ... DnaC [14] Rather the role of ATP in the reaction is to serve as a switch that controls the activity ofthe helicase The ATP bound form of DnaC severely inhibits the helicase activity of DnaB and ... by unwinding of this intrinsically less stable region of duplex [6] A further level of complexity arises from the fact that DnaA is a member ofthe AAA+ family of ATPases This class of protein...
... loss, within affected cells, ofthe two alleles of a given CCM gene Loss of one ofthe alleles (first hit) would be the result of a germline mutation and loss ofthe second allele (second hit) ... clinical care ofthe patients and were an important step towards the understanding ofthe mechanisms of this disorder This minireview summarizes the advances in CCM molecular genetics and the remaining ... K569E, were detected in the CCM lesion and were shown to be absent in the blood ofthe patient However, the data were difficult to interprete because ofthe nature ofthe mutations which were...
... earlier, themolecular identity ofthe protein(s) that actually form the PTP channel remains the most intriguing question On the basis of structural and other information, we hypothesize that the ... increased the conductance of artificial lipid bilayers in the presence of Ca2+, and the conductance was sensitive to ruthenium red [22], implying that it may be the Ca2+ uniporter or part thereof Further ... is driven by the pH gradient, in the case ofthe Ca2+ ⁄ 2H+ exchanger Elevated intramitochondrial Ca2+ can stimulate the activities of enzymes ofthe tricarboxylic acid cycle (TCA), thereby boosting...
... Evolution oftheMolecular Biology of Brain Tumors and the Therapeutic Implications high grade gliomas The structure and compounds ofthe ECM ofthe brain tissue have many specific differences from other ... mortality ofthe patient Moreover, the presence ofthe blood-brain barrier (BBB) limits the passage of a wide variety of anticancer agents The high incidence of recur‐ rence and poor prognosis of malignant ... Hofmann M Characterisation ofthe murine gene encoding the intracellular hyaluronan receptor IHABP (RHAMM) Gene 1999;226:41 23 24 Evolution oftheMolecular Biology of Brain Tumors and the Therapeutic...
... characterized the contribution of these assay components by omitting them individually In the absence of ferredoxin the reaction rate decreased to 46.5% ofthe complete reaction while the absence of ascorbate ... Arrows indicate the direction ofthe major changes in absorption over the course ofthe measurements (C) Michaelis– Menten plot ofthe PsHO1 reaction for heme concentrations of 1, 2, 5, 10 and ... region of Arabidopsis thaliana HO1 (AtHO1) and was used as the basis for thedesignof gene specific primers The 5¢- and 3¢-ends ofthe pea HO1 cDNA were obtained by rapid amplification of cDNA...
... on the basis ofthe P value of 0.005 (ii) What is the probability of obtaining a correlation coefficient so far from zero for at least ofthe cancer cell types in the panel if the null hypothesis ... http://genetics.nature.com ofthe 118 drugs We then clustered the 118 drugs on the basis of tered paradoxically with the alkylating agents, perhaps implying that drug metabolism rather than mechanism of action is an these ... mechanisms of action based on their activity patterns across the 60 cell lines b, Cluster tree ofthe 118 drugs based on the correlation of their activity patterns with expression patterns ofthe genes...
... 1.1 The process is initiated at the top left ofthe Vee with the definition ofthe operational need ofthe stakeholders The focus ofthe decomposition and definition process (or design) is the ... with the identification ofthe need for the system and progressing through the retirement ofthe system Some ofthe phases ofthelife INTRODUCTION TO SYSTEMS ENGINEERING Identification of Need ... 80% ofthe cost ofthe system is committed by the end ofdesign and integration, while only about 20% ofthe actual cost for the system has been spent Obviously, mistakes made in the front end of...
... Professor ofthe History of Science and Medicine at the University of Chicago Among his publications are Darwin and the Emergence of Evolutionary Theories of Mind and Behavior, The Meaning of ... had the patience to read through the entire manuscript The deep knowledge of these scholars ranged from the history of science to contemporary biology, from the logic of argument to the logic of ... however, the aspirations ofthe liberals crashed upon the failure ofthe Frankfurt Parliament Periodically through the 1830s and 1840s, students in the new Burschenschaften (the fi rst of these...
... years It was the mother who answered the questionnaire in 92.52% ofthe sample, and it was the father in 7.48% ofthe sample No parent in the patient group refused to participate in the study, ... questionnaire in 89.5% ofthe sample, it was the father in 4.57% ofthe sample, and it was someone else in 6.28% ofthe sample Distribution of all participants in terms of gender and age group ... Score, the mean is computed as the sum ofthe items divided by the number of items answered in the Emotional, Social, and School Functioning Subscales The sequential validation procedure of the...
... ofthe time Some ofthe time All ofthe time Knee pain present at night: None ofthe time Some ofthe time All ofthe time Knee pain worse on movement: None ofthe time Some ofthe time All of ... on the manuscript IR contributed to thedesignofthe study and commented on the manuscript WG contributed to thedesignofthe study and commented on the manuscript DK contributed to thedesign ... practice The lack of standardisation in the choice of instruments limits the generalisability of results Furthermore, ofthe five instruments included in the review that were based on the views of...
... scores ofthe DQOL were moderately correlated with the D-39S (except sexual functioning) and the RAND-12 Magnitudes in the correlations of all DQOL domains with the physical relevant domains ofthe ... among domains ofthe DQOL with the D-39S and the RAND-12 As described in the Introduction, because the DQOL essentially measures satisfaction and impact of diabetes treatment, whereas the D-39S measures ... discriminant validity ofthe DQOL against the D-39S and the RAND-12 In general, domain scores ofthe DQOL were moderately correlated with the D-39S (except sexual functioning) and the RAND-12 PHC and...
... the ED and at the time of follow-up (FU) Another consideration is validity: the strength ofthe association between the candidate instrument and other relevant outcomes (construct validity) The ... correlated with the PedsQL™ total scores at FU, with the exception of caregiver missed work or school (Table 1) The negative direction ofthe correlation reflects that the longer the duration of fever ... analyzed by means of a paired t-test The effect size was then calculated by dividing the overall mean change in score by the standard deviation ofthe score at baseline As a point of reference, an...
... Finally, there was no report by the patients or guardians of any lack of comprehension regarding the questions The lack of validation studies on assessment scales ofthe quality oflife among ... individuals off treatment Therefore, the occurrence of illness implied limitations and difficulties The analysis ofthe Corrected Item-Total Correlation proved the satisfactory homogeneity ofthe instrument ... treatment on the quality oflifeof patients Moreover, decisions for the implementation of improvements in public healthcare may be adopted based on the impact of interventions on quality oflife [1]...
... cross-cultural adaptation, the group of specialists stated that the concept ofthe impact of childhood cancer on the quality oflifeofthe families used for development ofthe original instrument was ... Page of (page number not for citation purposes) Health and Quality ofLife Outcomes 2008, 6:35 the health-related quality oflifeof parents/guardians The score is computed as the sum ofthe 20 ... illness, the assessment ofthe heath-related quality oflifeof parents and families could contribute toward their healthcare needs [3,21,22] This study presents the reliability and validity of the...
... Comparisons to the mean scores ofthe other subscales within the present study to those ofthe previous PedsQL™ school study [3], the mean scores on the social functioning scale of both healthy ... as the sum ofthe items divided by the number of items answered (this accounts for missing data) The physical health summary score is the same as the physical functioning subscale To create the ... summary score, the mean is computed as the sum ofthe items divided by the number of items answered in the emotional, social, and school functioning subscales If more than 50% ofthe items in a...