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the molecular design of life

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc

Báo cáo khoa học

... in the one-cell stage to visualize the localization and to measure the mobility of the components in the PGCs The medaka embryo was peeled off the chorion, and the segment containing the part of ... of OlvasGFP reveals the dynamic nature of the nuage Schematic diagram of the preparation of PGCs of medaka specimen (A) OlvasGFP was expressed in the medaka PGC at stage 24 and localized in the ... outside of the nuage in the cytoplasm of the migrating PGC FCS analysis revealed that Olvas GFP diffused with a diffusion constant of 7.0 lm2ặs)1 (Fig 3A) During the measurement of the PGC, the 10...
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Tài liệu Báo cáo khoa học: Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis docx

Tài liệu Báo cáo khoa học: Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis docx

Báo cáo khoa học

... (pTDN1) [14] The lack of characterization of the structural determinant of the substrate specificity of the AtdA enzyme has thus limited its development as a biocatalyst for the bioremediation of a wide ... reported which of the five genes control the substrate specificity of the AtdA enzyme The objective of this study was to identify and probe the residues determining the activity as well as the substrate ... may indicate that the steric limitation of the enzyme’s binding pocket takes place in the area between the ortho and para positions of the aromatic substrate On the basis of these results, aniline...
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Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx

Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx

Báo cáo khoa học

... wild-type ALP I On the other hand, the Tm of D266N/Y269A-ALP I increased by only °C The denaturation temperature indicates the stability of the structure of the protein The difference of the increments ... surrounding residues of the depression, and the side chain of the mutated residue might restrain the mobility of the N-terminal region on the surface by interaction with the main chain of Lys26 by van ... the substitutions did not influence the activities or specificity of the mutant enzymes The N-terminal region of ALP I was also on the surface The side chains of Ile10 and Tyr11 were close to the...
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Báo cáo khoa học: Molecular design of a nylon-6 byproduct-degrading enzyme from a carboxylesterase with a b-lactamase fold ppt

Báo cáo khoa học: Molecular design of a nylon-6 byproduct-degrading enzyme from a carboxylesterase with a b-lactamase fold ppt

Báo cáo khoa học

... Tyr170-Ca, and the side chain of Tyr170 is rotated Through the combined effect, ˚ Tyr170-Og moves a total of approximately 11 A, resulting in the formation of hydrogen bonds with the nitrogen of the amide ... simplify the estimation of the specific activity of each enzyme, we quantified the amount of Hyb-24-related protein and Ald-hydrolytic activity in cell extracts, and normalized the data on the basis of ... binding at the C-terminal region of Ald is improved by the D370Y substitution The roles of Asn266 and Tyr370 were further examined by site-directed mutagenesis Roles of Asn266 Superimposition of Hyb-24DNY...
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Báo cáo khoa học: Transthyretin and familial amyloidotic polyneuropathy Recent progress in understanding the molecular mechanism of neurodegeneration pdf

Báo cáo khoa học: Transthyretin and familial amyloidotic polyneuropathy Recent progress in understanding the molecular mechanism of neurodegeneration pdf

Báo cáo khoa học

... the location of amyloidogenic mutations and position of b-strands (A) The structure of the polypeptide backbone of the two chains (purple and blue) is shown along with the location of the N-terminus ... tissue and that the distribution of TTR deposition reflects the presence of these endogenous factors In the case of the Ab protein of Alzheimer’s disease, a number of proteins and factors (pathological ... these diseases, in particular Alzheimer’s disease, may help to explain some aspects of the pathogenesis of FAP The idea is discussed further in the following sections Tissue-specific pattern of...
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Báo cáo khoa học: Origins of DNA replication in the three domains of life pdf

Báo cáo khoa học: Origins of DNA replication in the three domains of life pdf

Báo cáo khoa học

... itself both at the level of the immediate chromatin environment of the intergenic region, and also at the level of topological status of the DNA as a result of transcription of the adjacent genes ... DnaC [14] Rather the role of ATP in the reaction is to serve as a switch that controls the activity of the helicase The ATP bound form of DnaC severely inhibits the helicase activity of DnaB and ... by unwinding of this intrinsically less stable region of duplex [6] A further level of complexity arises from the fact that DnaA is a member of the AAA+ family of ATPases This class of protein...
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Báo cáo khoa học: Recent insights into cerebral cavernous malformations: the molecular genetics of CCM pot

Báo cáo khoa học: Recent insights into cerebral cavernous malformations: the molecular genetics of CCM pot

Báo cáo khoa học

... loss, within affected cells, of the two alleles of a given CCM gene Loss of one of the alleles (first hit) would be the result of a germline mutation and loss of the second allele (second hit) ... clinical care of the patients and were an important step towards the understanding of the mechanisms of this disorder This minireview summarizes the advances in CCM molecular genetics and the remaining ... K569E, were detected in the CCM lesion and were shown to be absent in the blood of the patient However, the data were difficult to interprete because of the nature of the mutations which were...
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Báo cáo khoa học: The molecular identity of the mitochondrial Ca2+ sequestration system potx

Báo cáo khoa học: The molecular identity of the mitochondrial Ca2+ sequestration system potx

Báo cáo khoa học

... earlier, the molecular identity of the protein(s) that actually form the PTP channel remains the most intriguing question On the basis of structural and other information, we hypothesize that the ... increased the conductance of artificial lipid bilayers in the presence of Ca2+, and the conductance was sensitive to ruthenium red [22], implying that it may be the Ca2+ uniporter or part thereof Further ... is driven by the pH gradient, in the case of the Ca2+ ⁄ 2H+ exchanger Elevated intramitochondrial Ca2+ can stimulate the activities of enzymes of the tricarboxylic acid cycle (TCA), thereby boosting...
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EVOLUTION OF THE MOLECULAR BIOLOGY OF BRAIN TUMORS AND THE THERAPEUTIC IMPLICATIONS pdf

EVOLUTION OF THE MOLECULAR BIOLOGY OF BRAIN TUMORS AND THE THERAPEUTIC IMPLICATIONS pdf

Cao đẳng - Đại học

... Evolution of the Molecular Biology of Brain Tumors and the Therapeutic Implications high grade gliomas The structure and compounds of the ECM of the brain tissue have many specific differences from other ... mortality of the patient Moreover, the presence of the blood-brain barrier (BBB) limits the passage of a wide variety of anticancer agents The high incidence of recur‐ rence and poor prognosis of malignant ... Hofmann M Characterisation of the murine gene encoding the intracellular hyaluronan receptor IHABP (RHAMM) Gene 1999;226:41 23 24 Evolution of the Molecular Biology of Brain Tumors and the Therapeutic...
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Báo cáo khoa học: The molecular basis of heme oxygenase deficiency in the pcd1 mutant of pea pot

Báo cáo khoa học: The molecular basis of heme oxygenase deficiency in the pcd1 mutant of pea pot

Báo cáo khoa học

... characterized the contribution of these assay components by omitting them individually In the absence of ferredoxin the reaction rate decreased to 46.5% of the complete reaction while the absence of ascorbate ... Arrows indicate the direction of the major changes in absorption over the course of the measurements (C) Michaelis– Menten plot of the PsHO1 reaction for heme concentrations of 1, 2, 5, 10 and ... region of Arabidopsis thaliana HO1 (AtHO1) and was used as the basis for the design of gene specific primers The 5¢- and 3¢-ends of the pea HO1 cDNA were obtained by rapid amplification of cDNA...
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A gene expression database for the molecular pharmacology of cancer pptx

A gene expression database for the molecular pharmacology of cancer pptx

Cơ sở dữ liệu

... on the basis of the P value of 0.005 (ii) What is the probability of obtaining a correlation coefficient so far from zero for at least of the cancer cell types in the panel if the null hypothesis ... http://genetics.nature.com of the 118 drugs We then clustered the 118 drugs on the basis of tered paradoxically with the alkylating agents, perhaps implying that drug metabolism rather than mechanism of action is an these ... mechanisms of action based on their activity patterns across the 60 cell lines b, Cluster tree of the 118 drugs based on the correlation of their activity patterns with expression patterns of the genes...
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buede - the engineering design of systems - models and methods 2e (wiley, 2009)

buede - the engineering design of systems - models and methods 2e (wiley, 2009)

Kỹ thuật lập trình

... 1.1 The process is initiated at the top left of the Vee with the definition of the operational need of the stakeholders The focus of the decomposition and definition process (or design) is the ... with the identification of the need for the system and progressing through the retirement of the system Some of the phases of the life INTRODUCTION TO SYSTEMS ENGINEERING Identification of Need ... 80% of the cost of the system is committed by the end of design and integration, while only about 20% of the actual cost for the system has been spent Obviously, mistakes made in the front end of...
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the tragic sense of life-ernst haeckel and the struggle over evolutionary thought

the tragic sense of life-ernst haeckel and the struggle over evolutionary thought

Sinh học

... Professor of the History of Science and Medicine at the University of Chicago Among his publications are Darwin and the Emergence of Evolutionary Theories of Mind and Behavior, The Meaning of ... had the patience to read through the entire manuscript The deep knowledge of these scholars ranged from the history of science to contemporary biology, from the logic of argument to the logic of ... however, the aspirations of the liberals crashed upon the failure of the Frankfurt Parliament Periodically through the 1830s and 1840s, students in the new Burschenschaften (the fi rst of these...
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báo cáo hóa học:

báo cáo hóa học: " Measuring health-related quality of life in Hungarian children with heart disease: psychometric properties of the Hungarian version of the Pediatric Quality of Life Inventory™ 4.0 Generic Core Scales and the Cardiac Module" pptx

Hóa học - Dầu khí

... years It was the mother who answered the questionnaire in 92.52% of the sample, and it was the father in 7.48% of the sample No parent in the patient group refused to participate in the study, ... questionnaire in 89.5% of the sample, it was the father in 4.57% of the sample, and it was someone else in 6.28% of the sample Distribution of all participants in terms of gender and age group ... Score, the mean is computed as the sum of the items divided by the number of items answered in the Emotional, Social, and School Functioning Subscales The sequential validation procedure of the...
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báo cáo hóa học:

báo cáo hóa học: " Development of the Knee Quality of Life (KQoL-26) 26-item questionnaire: data quality, reliability, validity and responsiveness" pptx

Hóa học - Dầu khí

... of the time Some of the time All of the time Knee pain present at night: None of the time Some of the time All of the time Knee pain worse on movement: None of the time Some of the time All of ... on the manuscript IR contributed to the design of the study and commented on the manuscript WG contributed to the design of the study and commented on the manuscript DK contributed to the design ... practice The lack of standardisation in the choice of instruments limits the generalisability of results Furthermore, of the five instruments included in the review that were based on the views of...
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báo cáo hóa học:

báo cáo hóa học: " Evaluating the reliability, validity and minimally important difference of the Taiwanese version of the diabetes quality of life (DQOL) measurement" potx

Hóa học - Dầu khí

... scores of the DQOL were moderately correlated with the D-39S (except sexual functioning) and the RAND-12 Magnitudes in the correlations of all DQOL domains with the physical relevant domains of the ... among domains of the DQOL with the D-39S and the RAND-12 As described in the Introduction, because the DQOL essentially measures satisfaction and impact of diabetes treatment, whereas the D-39S measures ... discriminant validity of the DQOL against the D-39S and the RAND-12 In general, domain scores of the DQOL were moderately correlated with the D-39S (except sexual functioning) and the RAND-12 PHC and...
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báo cáo hóa học:

báo cáo hóa học: " Health-related quality of life for pediatric emergency department febrile illnesses: an Evaluation of the Pediatric Quality of Life Inventory™ 4.0 generic core scales" docx

Hóa học - Dầu khí

... the ED and at the time of follow-up (FU) Another consideration is validity: the strength of the association between the candidate instrument and other relevant outcomes (construct validity) The ... correlated with the PedsQL™ total scores at FU, with the exception of caregiver missed work or school (Table 1) The negative direction of the correlation reflects that the longer the duration of fever ... analyzed by means of a paired t-test The effect size was then calculated by dividing the overall mean change in score by the standard deviation of the score at baseline As a point of reference, an...
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báo cáo hóa học:

báo cáo hóa học: " Measurement properties of the Brazilian version of the Pediatric Quality of Life Inventory (PedsQL™) cancer module scale" pdf

Hóa học - Dầu khí

... Finally, there was no report by the patients or guardians of any lack of comprehension regarding the questions The lack of validation studies on assessment scales of the quality of life among ... individuals off treatment Therefore, the occurrence of illness implied limitations and difficulties The analysis of the Corrected Item-Total Correlation proved the satisfactory homogeneity of the instrument ... treatment on the quality of life of patients Moreover, decisions for the implementation of improvements in public healthcare may be adopted based on the impact of interventions on quality of life [1]...
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Báo cáo hóa học:

Báo cáo hóa học: "The Pediatric Quality of Life Inventory™ (PedsQL™) family impact module: reliability and validity of the Brazilian version" pdf

Điện - Điện tử

... cross-cultural adaptation, the group of specialists stated that the concept of the impact of childhood cancer on the quality of life of the families used for development of the original instrument was ... Page of (page number not for citation purposes) Health and Quality of Life Outcomes 2008, 6:35 the health-related quality of life of parents/guardians The score is computed as the sum of the 20 ... illness, the assessment of the heath-related quality of life of parents and families could contribute toward their healthcare needs [3,21,22] This study presents the reliability and validity of the...
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Báo cáo hóa học:

Báo cáo hóa học: " Validation of the Korean version of the pediatric quality of life inventory™ 4.0 (PedsQL™) generic core scales in school children and adolescents using the rasch model" ppt

Hóa học - Dầu khí

... Comparisons to the mean scores of the other subscales within the present study to those of the previous PedsQL™ school study [3], the mean scores on the social functioning scale of both healthy ... as the sum of the items divided by the number of items answered (this accounts for missing data) The physical health summary score is the same as the physical functioning subscale To create the ... summary score, the mean is computed as the sum of the items divided by the number of items answered in the emotional, social, and school functioning subscales If more than 50% of the items in a...
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