the mechanism of fermentation
Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... to study the binding of ATP alone to the protein were unsuccessful,as the protein precipitated upon addition of ATP in the absence of pantoate. An important feature of the NMR study is the fact ... [8,9]. One of the interesting features of the plant PSs is the presence of a conserved 24 residue insertion in the sequence [9].This sequence of amino acids, which is missing in the sequence of bacterial ... pantothe-nate from low amounts of pantoate [9]. Here, wereport the results of the structural studies of the panto-ate-bound form of the N-terminal domain of E. coliPS (nPS). The structure and substrate-binding...
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Tài liệu Báo cáo khoa học: On the mechanism of action of the antifungal agent propionate Propionyl-CoA inhibits glucose metabolism in Aspergillus nidulans doc
... for the initial decrease of the oxalo-acetate concentration in t he equilibrium due to the accumulation of NADH [25]. Lineweaver–Burk diagramswere obtained b y use of the worksheet of the programEXEL98 ... propionyl-CoA in the DmcsA-strain, the activity of methylisocitrate lyase is twice as high than that of the wild-type. The addition of acetate to these media notonly lowered the propionyl-CoA ... branch of the pentose phosphate pathway. Thiswas determined by the use of glucose-6-phosphate as the substrate and NADP as the hydrogen acceptor. Due to the use of cell-free extracts, not only the...
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Tài liệu Báo cáo khoa học: The mechanism of a-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase doc
... somedeviation from the geometry optimal for the abstraction of the a-proton. For the reaction of TPL withL-methio-nine the rate of abstraction of the a-proton, leading toformation of the quinonoid ... (b) the statistical factor taking account of the ratio of protons and deuterons on the considered groupwhen the latter is polyprotic; (c) the degree of restriction of the free rotation of the ... positions of the a-proton and the sidechain of any bound amino ac id. The lability of the a-protonobserved for a large number of amino acids [5] under the action of TPL gives evidence for the orthogonal...
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Tài liệu Báo cáo khoa học: A role for the intersubunit disulfides of seminal RNase in the mechanism of its antitumor action docx
... mechanism of antitumouraction of BS-RNase is based on the ability of the proteinto resist the neutralizing action of the cytosolic RNaseinhibitor, a resistance due to the dimeric structure of the enzyme. ... indicate that the cell sulfhydryls responsiblefor the exchange with the protein disulfides are located in the plasma membrane. Furthermore, they show that, asproposed in the hypothesis above, the RNase ... hypothesize that the cytotoxicactivity of the latter monomers was due to the presence inthese proteins of disulfide bonds, with their potentialchemical instability. It is well known that in the...
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Báo cáo y học: "Ultra-low microcurrent in the management of diabetes mellitus, hypertension and chronic wounds: Report of twelve cases and discussion of mechanism of action"
... minutes, which then switched to the opposite polarity for another 11.5 minutes. The device was designed to switch the di-rection of current flow halfway through the cycle. The resulting cycle ... (67). Inter-ruption of the overproduction of superoxide by the mitochondrial electron transport chain would nor-malize the pathways involved in the development of the oxidative stress ... role in the pathogenesis of both types of diabetes mellitus and cardiovascular diseases including hypertension. The low levels of antioxidants accompanied by raised levels of markers of free...
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Tài liệu Báo cáo " Mechanism of forming the Dien Bien basin " pdf
... analyzed the data about the Dien Bien - Lai Chau fault zone activities, the geological structure of the basin, as well as the theory of basin-forming regime to figure out the mechanism of formation ... along the long axis of the basin. The rock of the east side has been broken much more than that of the west side. - The cover composed of olivine basalt and loose sediments: + The Late Neogene ... started. On the west side of the basement, the subsidence may take place so quick that the basement slope is steeper and the bed rock is more stable. In turn, on the other side the bedrock...
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Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
... deuteration of C2¢ of the epimerase product. The lack of deuteration of the C2¢ of DHMP is not due to the lack of deute-rons, because it has been shown previously that the 6-hydroxymethyl group of the ... set of control data was obtained in the absence of the protein. The data set obtained in the absence of the protein was then subtracted from the correspondingdata set obtained in the presence of ... performed in the absence of the ligand. The controldata set obtained in the absence of the ligand was subtrac-ted from the corresponding data set obtained in the pres-ence of the ligand. The Kdvalues...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... iscomposed of residues 25–540. The overall structure of the model of IPU and the mutated residues in this study areshown in Fig. 3. To elucidate the mechanism of the substrate recognition of IPU, ... panose (data notshown).Prediction of the positions of amino acid residues whosesubstitutions resulted in the reduction of the IPU activityWhile the study of site-directed mutagenesis describedabove ... in the negatively numberedsubsites. The anomeric configuration of products of bothDex49A and IPU are identical, as well. Does the substratepreference of the negatively numbered s ubsites of...
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Tài liệu Báo cáo khoa học: Unraveling the catalytic mechanism of lactoperoxidase and myeloperoxidase A reflection on some controversial features Elena Ghibaudi and Enzo Laurenti docx
... oxidize them [19]. On the other hand, this factdoes not represent proof of the presence of one or twooxidizing equivalents on the decay product of Cpd I. The EPR spectrum of a spin-trapped radical ... formulated a hypo-thesis that describes an integrated vision of the catalytic mechanism of both enzymes. The main points are: (a) are-evaluation of the role of superoxide as a reductant in the catalytic ... MPO. They dealwith the the major importance of catalase activity in MPO withrespect to LPO, the different sensitivity of the enzymes toCpd III inactivation and the redox potential of the chemicalintermediates....
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Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx
... treeswith the nt sequence of the open reading frame (ORF) of their cDNAs and the corresponding aa sequences, inTable 1. Summary of the purification of DNases I from the pancreases of three species of ... cloned the cDNA of each. A single amino acid(aa) substitution was confirmed to affect the thermalstabilities of vertebrate DNases I and, furthermore, one of the postulated mechanisms whereby thermal ... not. The same was true for these mutants of the rat and mouseDNase I (Fig. 3). These findings demonstrate that the nature of the amino acid at position 130 may generally andmarkedly affect the thermal...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... coliagmatinase, of the loop indicated by the letter a.Fig. 5. Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type ... indicating that the environ-ment of tryptophan residues is essentially conserved in the mutant enzyme. On the other hand, the absence of majordifferences in the CD spectra of the wild-type and ... further evaluate the consequences of the Asp153fiAsn substitution, we used amodelled structure of the E. coli enzyme, constructed byusing homology-modelling techniques and the 3D structure of the...
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- the adventure of physics
- the nature of language
- the distribution of zeros of entire functions
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- ebook the lake of souls
- know the history of language
- the regulation mechanism of the systems is programmed in each component
- the effect of microorganisms and fermentation by microorganisms
- the production of ethanol by fermentation
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