the interaction of saccharides with antibodies a 3d view by using nmr
Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... melibiose and a- Me-Gal, as anomers of lactose and b-Me-Gal, was in the intermediate exchange regime Thus, the configuration at the hemiacetal carbon of galactose may affect the dissociation constants By ... exchange, of which the Kd should have a medium value between that in the slow exchange regime and that in the fast exchange regime, titration data indicated that the interaction with sugars had a Kd of ... domain of EW29 at pH 6.1 and 298K in the sugar-free state Cross-peaks are labeled based on an analysis of through-bond connectivities The side chains of NH2 resonances of asparagines and glutamines...
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Báo cáo Y học: Exploration of the diaphorase activity of neutrophil NADPH oxidase Critical assessment of the interaction of iodonitrotetrazolium with the oxidase redox components doc
... NADPH oxidase activity was assayed in a cell-free system [13], either by measurement of the rate of production of O2– or the rate of O2 uptake INT diaphorase activity was assayed by the rate of ... stable at 25 °C in hydrophobic media, even in the presence of O2 The tetrazolinyl radical can also accumulate by oxidation of formazan or by disproportionation of a mixture of formazan and the ... by the rate of reduction of INT (j) A parallel experiment was carried out in which 5-deaza FAD was incubated for with the membrane suspension after the cell-free activation of the NADPH oxidase;...
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Báo cáo khoa học: The mystery of nonclassical protein secretion A current view on cargo proteins and potential export routes pdf
... secreted early after monocyte activation and HMGB1 at a later stage IL-1b consistently acts at an early phase of inflammation whereas HMGB1 functions as a late mediator of inflammation (see above) These ... proteins in mammalian cells revealed the existence of a machinery that is capable of translocating the protein across the plasma membrane [103], demonstrating a conserved pathway among lower and higher ... causes galectin-1 and galectin-3 to accumulate at specific spots underneath the plasma membrane, and what actually causes the formation of exosomes into which these proteins appear to be packaged...
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THE POSSIBILITY OF ELIMINATING METAL IONS FROM INDUSTRIAL SEWAGE BY USING ALUMINUM HYDROXIDE
... that could impact the results are the particle size of the aluminum hydroxide The particle size of the aluminum hydroxide is not the same and each time the size of the particle was added are have ... measurement, and the calibration linear is passing through the original point of the X-Y axis Details of measured data can checking the Appendix Table 11 Ion’s concentration in the sewage after adding ... Nowadays, there are plenty of types of AAS that be utilizing, and among all of them, flame atomizer’s AAS is the most common one that can be utilized for analyzing There are also several combinations...
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Báo cáo khoa học: A spectroscopic study of the interaction of isoflavones with human serum albumin pdf
... scans are an average of three runs A mean residue weight of 115 was used for calculating the molar ellipticity values (A) Effect of warfarin on the near UV CD of HSA The concentration of HSA was ... to HSA Studies with fatty acid Among the various ligands, fatty acids alone can attach to the primary binding site of HSA Experiments have been conducted using palmitic acid and defatted HSA to ... characteristic of albumin to allow a variety of ligands to bind to it is amazing Albumin is the principal carrier of fatty acids that are otherwise insoluble in the circulating plasma Human serum albumin...
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Báo cáo y học: "Direct interaction of immunoglobulins with synovial fibroblasts: a missing link in the pathogenesis of rheumatoid arthritis" potx
... destruction Although a number of molecular pathways have been identified that contribute to the stable activation of RASFs, the precise cause and nature of this activation, as well as its relevance and ... research on the interaction of RA-IgG and RASFs as well as other recent data, however, may change the picture It has been reported by Huizinga and colleagues that in a cohort of patients with recent ... consequences, are matters of debate The present data indicate very clearly that stable alterations in the fibroblasts themselves are indispensable for (auto )antibodies to exert their effects on IL-16 (and...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... arginase activity of the Asn149Asp variant was practically undetectable, both before and after the incubation with the manganese ions Fully active His120Asn and His145Asn variants exhibited about ... 5% of the arginase activity of the wildtype enzyme As measured by kcat ⁄ Km, the catalytic efficiency of the Asn149Asp variant was found to be about 36-fold lower than that of wild-type arginase ... preparation of a substrate complex of Bacillus caldovelox arginase for crystallographic analysis [32] According to our present results, the catalytic activity of the partially active species of arginase...
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Đề tài " The distribution of integers with a divisor in a given interval " ppt
... of Mathematics and Informatics, Bulgarian Academy of Sciences Finally, the author acknowledges the referee for a thorough reading of the paper and for helpful suggestions This work was partially ... (7.2) write a = a1 a2 with pβ , a1 = pβ a, β≥2 so that (a1 , a2 ) = and a2 is square-free By Lemma 3.1, L (a; σ) ≤ τ (a1 )L (a2 ; σ) ≤ στ (a1 )τ (a2 ) (7.6) Also (7.7) a1 τ (a1 ) a1 >w −3/4 a1 w−1/4 ... divisors of n to lie all above their expected values An analogy from probability theory is to ask for the likelihood that a random walk on the real numbers, with each step haveing zero expectation,...
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Đề tài " On the periods of motives with complex multiplication and a conjecture of GrossDeligne " pdf
... in a very general setting Recall that a period of an algebraic variety defined by polynomial equations with algebraic coefficients is the integral of an algebraic differential against a rational ... subvariety of X has a cycle class in the dual of a rational homology space of X and the duals of these cycle classes span a subspace of homology, which might be large Up to normalisation, the ... algebraic linear relations between the logarithm of the periods of the eigendifferentials of X (for the action of the group) and the logarithm of special values of the Γ-function (recall that they are...
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Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx
... of GC-rich random-template (lanes and 2), ATT-template (lanes and 4), AAT-template (lanes and 6) and : mixture of ATT- and AATtemplate Samples with (+) and without (–) DAPI are indicated Before ... produced by DAPI on the ATT-strand of the AATÆATT trinucleotide repeat are associated with the stalling of Klenow progression along the ATT-template sequence This draws attention to the biological ... does not appear to significantly a ect Klenow activity catalyzed on the AAT- and GC-rich random-templates The synthesis was stalled by DAPI on the ATT-bearing template giving rise to a prevalent...
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Báo cáo Y học: Biophysical characterization of the interaction of high-density lipoprotein (HDL) with endotoxins doc
... applied using a modified version of the CURFIT program obtained by D Moffat, NRC, Ottawa, Canada An estimate of the number of band components was obtained from deconvolution of the spectra [8] and the ... Parallel to the measurements of LPS Re, differential scanning calorimetry measurements of the phase behavior of lipid A indicated a similar increase in Tc, and the evaluation of the phase transition ... under a nitrogen stream at room temperature The lipid sample was placed in a closed cuvette, and the air above the sample was saturated with water vapor to maintain full hydration Infrared ATR...
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Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt
... expressing either a TAP tag alone, N-terminally (N-TAP) or C-terminally (C-TAP) TAP-tagged Vg1RBP was incubated with IgG-sepharose beads and eluted by TEV protease cleavage of the TAP tag Eluted ... percent of the oocyte extract was used for the isolation of total RNA using the same protocol RNA was reverse transcribed into cDNA and analyzed by quantitative PCR Enrichment factors were calculated ... join the localization complex in the nucleus and mediate anchoring to cortical actin upon arrival of the RNP at the vegetal pole of the oocyte In the context of these localizing RNPs, but also as...
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Báo cáo khoa học: The influence of cholesterol on the interaction of HIV gp41 membrane proximal region-derived peptides with lipid bilayers potx
... has a + net formal charge, and the negatively charged lipid For MPRP-I, the partition constant increase was not as high and in the case of MPRP-S the partition constant remained unchanged relative ... Soares CM, Baptista AM, Goormaghtigh E, Costa Cabral BJ & Castanho MARB (2006) Conformational and orientational guidance of the analgesic dipeptide kyotorphin induced by lipidic membranes: putative ... region derived peptides A S Veiga and M A R B Castanho The membrane interaction behaviour of MPRP-C and MPRP-I is independent of the membrane phase but not of the presence of charged lipids Cholesterol...
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Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA ppt
... Biolabs, Beverly, MA, USA) Amplifications were performed using Pfu DNA polymerase and oligonucleotides MALN1: 5¢-GC GGAATTCAAAAAGAGAAAGAAAGGG-3¢ and MALC: 5¢-GGCCTGCAGCTAGAGAAGTGAAACATC-3¢ with HAKN1 ... rounds of amplification with oligonucleotides 199 KNOX homeodomain–DNA interactions MAT1 (5¢-AGGGGACATAGATTTACAAAAGAAGCTC GTCAACAA-3¢; first round, MATa2 sequences underlined) or MAT2 (5¢-CGCGAATTCAAGCCGTACAGGGGAC ... (5¢-TTCCTTTGGTTTTTGAACCAGTTG-3¢), K54VF (5¢TAAACCARAGGGTGCGGCAYTGGA-3¢), K54VR (5¢TCCARTGCCGCACCCTYTGGTTTA-3¢), R55AF (5¢-CA AAGGAAGGCGCACTGGAA-3¢), R55AR (5¢-TTCCAG TGCGCCTTCCTTTG-3¢), R55KF (5¢-CAAAGGAAGAA...
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A Study on the Effects of Argentine Tango as a Form of Partnered Dance for those with Parkinson Disease and the Healthy Elderly pptx
... suggested that the basal ganglia, the area of neurological degeneration in those with PD, are specifically involved in the control of dance movements Increased activity in the basal ganglia was observed ... group stated on the exit questionnaire what they liked best and least about the program They greatly appreciated the camaraderie and socialization engendered by the program Being able to meet others ... for their assistance with this study This work was supported by a grant from the Marian Chace Foundation to Madeleine Hackney and a grant from the American Parkinson Disease Association to Gammon...
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Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt
... ligands and aid their interaction with the PPIase active site Comparison of PPIase domains and the FK506rapamycin interaction The PPIase domain fold is highly conserved within the large family of ... ratio of : 1, as further additions of the largely water-insoluble agents caused the formation of a white precipitate, and no further change in the spectra was observed Although these data are not ... (Figs and S4) Moreover, a section of the PPIase core domain, at or near the catalytic pocket, was also affected A more detailed mapping analysis of the extent of the chemical shift variation...
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Báo cáo Y học: Tryptophan fluorescence study of the interaction of penetratin peptides with model membranes pdf
... was accompanied by at least a twofold decrease of the uorescence intensity and a decrease of the mean Trp lifetime, in contrast with the behaviour of other peptides [17] The three lifetimes of ... penetratins are attributed to the classical three rotamers of chi1 (CaCb) The average lifetime of the lipid-free WT-penetratin was calculated from the average lifetimes of the individual Trp ... Trp was moved along the sequence Interaction of these peptides with lipid vesicles is accompanied by an increase of the a helix conformation, a disappearance of the short uorescence lifetime, an...
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