the influence of physico chemical conditions on microbial communities
Tài liệu Báo cáo khoa học: Agrobacterium tumefaciens type II NADH dehydrogenase Characterization and interactions with bacterial and thylakoid membranes ppt
... seems to be regulated as a function of variations in the growth conditions [21] In chloroplasts of higher plants and algae, in addition to the photosynthetic electron transfer chain oxidizing water ... peaks of FAD-containing proteins were observed, confirming the nature of the cofactor [19] By using the extinction coefficient at 450 nm we calculated that the stoichiometric ratio of flavinic cofactor ... could grow under these conditions Note that partial complementation of the mutant was observed in the absence of IPTG, likely indicating significant IPTG-independent expression of the protein Using...
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Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt
... neuronal responses to elevated Ab levels in models of AD [8], suggesting that disruption of the association of these proteins could represent a potential therapeutic strategy for the treatment of ... treatment of WT neurons Numbers on the left indicate molecular masses (kDa) The bar charts below show the amount of tau in DRM fractions as a proportion of total tau in cell lysates, expressed as the ... downstream consequences Furthermore, these results support the view that non-microtubule associations of tau are important for normal physiological function in neurons, and reinforce the suggestion that...
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Tài liệu The Acquisition of Drugs and Biologics for Chemical and Biological Warfare Defense - Department of Defense Interactions with the Food and Drug Administration doc
... justify the use of INDs in actual conflict on the grounds that the benefits of IND use (or the The Challenges of Acquisition 23 costs of nonuse) exceed the risks, such a decision runs the risk of ... arises from the dependence of DoD on FDA decisions about drugs, the comprehensive nature of FDA regulation, the continual change in those regulations and in FDA’s interpretation of them, and the limited ... regulation of the same The education program should be pursued regardless of the organizational approach—GOCO or prime contractor—that DoD pursues for CBW defense The on the job” training of acquisition...
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Báo cáo khoa học: Hepatocyte growth factor activator (HGFA): molecular structure and interactions with HGFA inhibitor-1 (HAI-1) doc
... conformational states of the active site region: a catalytically competent (standard) conformation, and two nonstandard conformations Herein, we use the conformation seen in the complex of HGFA ... plasticity of the serine protease active site region Thus, it is reasonable to assume that the unconventional 99-loop and 220-loop conformations of HGFA are part of an ensemble of conformational states, ... uPA A likely reason is the conformation of the uPA 99-loop Although it is only one amino acid longer than the 99loop in HGFA, its conformation is quite different and, in a hypothetical complex,...
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Báo cáo khoa học: Krit 1 interactions with microtubules and membranes are regulated by Rap1 and integrin cytoplasmic domain associated protein-1 doc
... Krit1 at the micromolar concentrations used in these experiments and, for the second part, with the higher contribution to the binding to MT of the basic stretch than the C-terminal site At the cell ... members of the FERM family undergo intra and ⁄ or inter molecular folding of their C-terminus onto their N-terminal FERM domain [21] Consistently, we demonstrate the interaction of the N-terminus of ... 2A) The resulting GST-HypoKrit1DC mutant has a truncation of half of its F3 subdomain We used the same experimental conditions as in Fig 3A Deletion of the C-terminus of Krit1 abolished the binding...
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Báo cáo khoa học: Cdc37 maintains cellular viability in Schizosaccharomyces pombe independently of interactions with heat-shock protein 90 doc
... location of the nucleotide insertion Four mutants obtained contained in-frame insertions resulting in the insertion of five amino acids into the Cdc37 protein Stop codons were inserted by the five ... Boxes with horizontal stripes indicate the location of the postulated Hsp90 binding domain and the box with diagonal stripes denotes the homodimerization domain (C) Truncation mutants of S pombe Cdc37 ... Fragments of cdc37 containing insertions were identified by gel electrophoresis, cut out of the gel and purified, then ligated into the restriction sites Nde1 and Xma1 of pREP81 The transposon transprimer...
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Báo cáo Y học: Domain V of m-calpain shows the potential to form an oblique-orientated a-helix, which may modulate the enzyme’s activity via interactions with anionic lipid potx
... version of the CURFIT procedure written by D Moffat (National Research Council, Ottowa, Ontario, Canada) An estimation of the number of band components was obtained from deconvolution of the spectra; ... was then applied within the original spectra after the subtraction of baselines resulting from neighbouring bands Similar to the deconvolution technique, the bandshapes of the single components ... ability of VP1 was reduced the order of 60% (LD50 ¼ 1.85 mM) FTIR conformational analysis of VP1 FTIR spectroscopy was used for conformational analysis of VP1, either in aqueous solution or in the...
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Báo cáo khoa học: Deciphering the key residues in Plasmodium falciparum b-ketoacyl acyl carrier protein reductase responsible for interactions with Plasmodium falciparum acyl carrier protein pptx
... might be the reason for the greater conservation of Arg230 during evolution (Fig 1) In conclusion, our results provide compelling support for the hypothesis that the highly conserved a2 helix of PfACP ... rate of 20 lLÆmin)1 For the determination of dissociation rate constant, the same buffer was passed at a flow rate of 20 lLÆmin)1 To study the effect of NADPH on the PfFabG–PfACP interaction, PfACP ... Arg230 of PfFabG are involved in the interaction with PfACP These studies demonstrate that the highly conserved second a-helix of PfACP recognizes electropositive residues on the surface of PfFabG...
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Báo cáo khoa học: Biosynthesis of D-arabinose in mycobacteria – a novel bacterial pathway with implications for antimycobacterial therapy pdf
... biosynthesis and the mode of action of ethambutol Recent studies have confirmed that decaprenyl-phospho-arabinose is the only donor of the arabinofuranosyl residues for the synthesis of the cell-wall ... (b-d-arabinofuranosyl1-monophosphodecaprenol) is the only known donor of d-arabinofuranosyl units in the synthesis of arabinans of Actinomycetales Disruption of the gene encoding the 5-phospho-a-d-ribose ... in the synthesis of the vital arabinans in mycobacteria Formation of 5-phosphoribosyl-a-1-pyrophosphate The second step in the biosynthesis of decaprenylphospho-arabinose (Fig 2) is the reaction...
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Báo cáo Y học: ER–resident chaperone interactions with recombinant antibodies in transgenic plants potx
... expression of functional antibodies was found alongside a decrease in the formation of abnormal protein aggregates [48] One of the potential advantages of the plant expression system is the presence ... ligand–chaperone relationship between the two molecules Note that immunoprecipitation with anti BiP antiserum in all panels of Fig 4D leads to coselection of an unrelated polypeptide of the same size of the ... chaperone interaction with heterologous proteins has not been performed The aims of this study were to determine how the plant ER responds to the expression of heterologous secretory proteins We therefore...
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báo cáo hóa học: " Microglia use multiple mechanisms to mediate interactions with vitronectin; non-essential roles for the highly-expressed avb3 and avb5 integrins" pptx
... serum-free conditions for three days on vitronectin Consistent with previous results, fibronectin and vitronectin promoted strong induction of pro-MMP-9 compared to the uncoated plastic control substrate ... each condition For each experimental condition, the mean fluorescent intensity was compared with the control state and expressed as the percentage change relative to the control condition Each ... activation and phagocytic processes in other cell types, the purpose of the current study was to investigate the contribution of these two integrins in this process, and thereby test our hypothesis...
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Báo cáo y học: "Genetic polymorphisms in PTPN22, PADI-4, and CTLA-4 and risk for rheumatoid arthritis in two longitudinal cohort studies: evidence of gene-environment interactions with heavy cigarette smoking" potx
... interaction using an interaction variable (for example, gene × smoking) in the conditional logistic regression models The significance of the interaction was determined using the Wald χ2 test of the ... by the presence of RF among the RA cases, we employed unconditional logistic regression analyses, adjusting for each of the matching factors, in addition to the covariates above For analyses of ... proportion diagnosed by a member of the ACR All cases and controls in these analyses were Caucasian, and the mean (± standard deviation) number of ACR criteria for the classification of RA was...
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báo cáo khoa học: "Single-walled carbon nanotube interactions with HeLa cells" doc
... preparations and concentrations of purified DM-SWNTs not increase the concentrations of mitochondrial superoxide in HeLa cells under these culture conditions MitoSOX™ Red Fluorescence Intensity Conclusion ... sonicated for 10 at 0°C Probesonication was performed using a Branson 250 Sonifier, and the mm diameter probe tip was placed one-third of the distance below the surface of the mL suspension The ... Binding and Condensation of Plasmid DNA onto Functionalized Carbon Nanotubes: Toward the Construction of Nanotube-Based Gene Page 14 of 17 (page number not for citation purposes) Journal of Nanobiotechnology...
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báo cáo khoa học: " Dissecting grain yield pathways and their interactions with grain dry matter content by a two-step correlation approach with maize seedling transcriptome" pps
... 100 repetitions On average 190 of the 200 genes showing the strongest correlation with PY in the estimation set were among the set of the 200 genes with the strongest correlation in the complete ... subjected to further analyses The average number of genes differentially expressed between the parents of a hybrid was 3350, which equals 7.7% of the genes on the array (see Additional file 1) The mid-parent ... HY the average number of agreeing genes was 185 This result confirms that the different genetic backgrounds of the inbred lines only marginally contributed to the random error in the correlation...
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Báo cáo y học: " HIV interactions with monocytes and dendritic cells: viral latency and reservoirs" pptx
... allow study of only the long-term HIV-l reservoirs Role of monocytes Monocytes are implicated as a viral reservoir based on the detection of, or the recovery of, infectious virus from monocytes ... expression [94,97], therefore, they lack functional P-TEFb However, this is not the only factor responsible for the resistance of monocytes to HIV-1 replication, as transient expression of CycT1 ... suggestions on the manuscript We thank the members of the Wu laboratory for critical reading of the manuscript and helpful discussions The research of the Wu laboratory is supported by grants from the...
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Báo cáo y học: "Misfolding of CasBrE SU is reversed by interactions with 4070A Env: implications for gammaretroviral neuropathogenesi" ppsx
... upon synthesis This interaction results in CasBrE SU expression on the cell surface and facilitates its incorporation into viral particles In addition, the interaction results in the restoration ... virion incorporation The small amount of CasBrE SU noted after sedimentation of the CasES supernatant without 4070A represents trace amounts of medium containing high levels of SU rather than the ... DTT and only run in the second dimension indicated by the separate lane (left columns) to mark the migration of the different immunoreactive components C Non-reducing/reducing two-dimensional SDS-PAGE...
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Báo cáo y học: "Bench-to-bedside review: Bacterial pneumonia with influenza - pathogenesis and clinical implications" pdf
... apoptosis of inflammatory cells Although the contribution of these Page of This article is part of a review series on Influenza, edited by Steven Opal Other articles in the series can be found online ... http://ccforum.com/content/14/2/219 approaches mainly focus on the prevention of secondary bacterial pneumonia Patients with community-acquired pneumonia who demonstrate or have demonstrated signs and symptoms of ... disease during primary and secondary pneumococcal pneumonia, while colonization within the lung seems to be dependent on the bacterial strain [43-45] In conclusion, there is little evidence that...
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apoptotic b cells their interactions with macrophages and modulation by rituximab
... Activation of OTII T cells after secondary presentation of differing concentrations of RTX:OVA by BMDM 169 5.2.3 Comparison of activation of OVA-specific T cells after secondary presentation ... Recognition of pathogens via PRRs results in the initiation of multiple responses by innate cells The production of chemokines recruits additional effector cells to sites of inflammation, the secretion ... stimulation [59] and the activation state of the cell influencing the cytokine profile [60], demonstrating the context dependant nature of cytokine production by B cells Populations of regulatory...
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bowlin et al - 2014 - the effects of auditor rotation, professional skepticism, and interactions with managers on audit quality
... in the dishonesty frame conditions will sum to (not sum to) more than 100% in the no-rotation (rotation) conditions Consistent with the theory, within the no-rotation conditions, these complementary ... assessments of the honesty of management representations in the honesty assessment frame conditions, and 100% minus their probability assessments of the dishonesty of management representations in the ... Representations equals the auditors' probability assessments of the honesty of management representations in the honesty assessment frame conditions, and 100% minus their probability assessments of the...
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Assessment of management scenarios to reduce loggerhead turtle interactions with shrimp trawlers in georgia
... Upon release of the turtles, Geostationary Operational Environmental Satellites (National Oceanic and Atmospheric Administration) monitored the platform terminal transponder signals on a continuous ... Atlantic population of loggerhead turtles Caretta caretta; the Northern Recovery Unit extends from the Florida–Georgia border to the northern extent of the nesting range in Virginia The Northern Recovery ... they are used to make predictions about consequences of management actions (Conroy and Moore 2002) Use of loggerhead turtle movement data to forecast the outcomes of proposed management actions...
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