... reduces the pep-tide binding affinity by 100-fold andthe lifespan of the complex by 10-fold, as judged by koff. The physiologicalchaperone cycle probably consists ofthe rapid binding of the ... hypothesize that the proton-ated state ofthe chaperone initiates this cycle, whereas the deprotonated state occurs upon completion of the maturation process ofthe partner. The nature of the signal ... common feature of this newfamily of chaperones. It is worth mentioning that the function of these proteins is not restricted to the recog-nition and binding ofthe N-terminus ofthe nascentmetalloprotein,...
... capable of giving strength to the weary, vivacity to the stupid, and wisdomto men void of understanding; capable of soothing the sorrows ofthe afflicted, of healing the wounds of the spirit, and ... healthycondition ofthe vessels belonging to the membranes ofthe socket, with the condition of which, the state of the tooth is closely connected."]Having gone through with an examination ofthe physical ... towards them.Hence the Methodists interdicted the common use of tobacco with that of ardent spirit, in the infancy of theirsociety; thereby evincing a just sense ofthe self-denial, decency, and...
... Superposition ofthe active-siteresidues ofthe 20 conformers ofthe solu-tion structure of HtA. Catalytic groups E66 and H113 are shown in red, and side chains of other residues in their vicinity ... understanding the structural requirements for the general ribonucleolytic and cytotoxic activities ofthe protein HtA. With thisaim, we determined the structure of HtA by1H-NMRmethods, andthe ... Comparison ofthe spatial orientation of the N-terminal b-hairpin and loops 2 and 5 in HtA and a-sarcin. The backbone trace isrepresented in blue for the b-hairpin, orangefor loop 5, and green...
... ofthe Constitution to judicial decisions about the text, theorists insist that the warp and woof of constitutional law is still traceable to the written document and must be, given that the ... try again (and again and again) to succeed where they and others have failed before. My aim is primarily to organize the argu-ments and counterarguments and to expose the pretense of a documen-tary ... fed-eral and state governmental power) associated with the Due Process Clauses ofthe Fifth and Fourteenth Amendments andthe Equal Protec-tion Clause ofthe Fourteenth. But I also turn to the freedom...
... ligand binding. The loop region ofthe upper domainmoves towards the active site ofthe enzyme, thus shielding the substrates (and ligands) from bulk solvent. A detailedthermodynamic study of ... 1UAE [11]), conformation. The thiol group of C115 is highlighted in green and the nitrogen atoms of R120 and K22 in blue. The open form of MurA is unliganded (A) and the closed conformation (B) ... importance of R120 for the formation ofthe covalent fosfomycin adduct, we construc-ted the K22V/R120V and K22V/R120K double mutants(see Materials and methods) and repeated the analysis of adduct...
... the notions of extended tuning systems, the relation of tuning to timbre and spatiality of sound, andthe concentration of attention for extended time periods.” One may also generalize the ... romantic idea that in the golden ratio “is contained the fundamental principle of all formation striving to beauty and totality in the realm of nature and in the field ofthe pictorial arts.” ... bursting with false claims and misconceptions about the appearance ofthe Golden Ratio in the arts (e.g. in the works of Giotto, Seurat, Mondrian). The history of art has nevertheless shown that artists...
... the semantic classes C for both the head-word (wi) and hypernym(s) (wg). If at least one of the index word’s classes is subsumed by at leastone ofthe genus’ classes, then we consider the ... familiar-ity and part of speech, all the underlined featuresare those added by the Hinoki project.3 The Hinoki Treebank The structure of our treebank is inspired by the Redwoods treebank of English ... Entry for the Word doraib¯a “driver” (with English glosses)grammar in tandem with the treebank, as part of our research into natural language understanding.Treebanking the output ofthe parser...
... or other characteristics beyond the control of the country’s government, by the method used to aggregate individual indicators, by the com-parability ofthe data, and by the purpose ofthe ... indication of the lack of economic freedom in the compila-tion ofthe index, the average score ofthe Nor-dic countries on the overall economic freedom index is only slightly higher than that ofthe ... positions in the professions, legislative bodies, and senior civil service. In contrast to the United States, Finland ranks near the top ofthe industrialized world on each of the following...
... amount of land or personal property, the ability to read and write the constitution ofthe state or of the United States, andthe "Grandfather Clause" which permits one unable to meet the ... enjoyment ofthe fruits of his own labor andthe pursuit of happiness in his own way, aslong as he walks in the path of rectitude and duty and does not trespass upon the rights of others," ... choice of electors for president and vice-president ofthe United States,representatives in congress, the executive and judicial officers of a state, or the members ofthe legislaturethereof,...
... migrating bands of three LPS(without R14) and fast migrating bands of P. mirabilisG1, O 28, and R14 LPS (Fig. 5A). These bands correspondto high- and low-molecular-mass LPS species consisting of the ... migrating bands of allLPS tested and slow migrating bands of P. mirabilis S1959,G1, and O28 LPS, the banding patterns being clearlydifferent (Fig. 5B). These findings together showed that the epitope ... O-polysaccharide p art of LPS. The serological relatedness of P. mirabilis G1 and S 1959correlated with the similarity ofthe chemical structures of their O-polysaccharides (Fig. 3). Therefore, it is...
... RPAP. The size o f the interaction surface between the peptide andthe non binding bottom ofthe Fab is 173 A˚2which c orresponds to about 36% ofthe interaction of the full peptide with the ... MUC-1; binding epitope. The extracellular part ofthe epithelial glycoprotein MUC-1consists of tandem repeats of 20 amino acids(PDTRPAPGSTAPPAHGVTSA, where the start of the tandem repeat peptide ... ecrystalcellthathaveadistanceof4A˚or less to the ligand. The C-terminal part ofthe pe ptide (RPAP) has clo se contacts to the n ext protein in the crystal cell and is therefore stabilized on the surface o...
... The Majors of GolfComplete Results ofThe Open, the U.S. Open, the PGA Championship and the Masters, 1860–2008MORGAN G. BRENNERVolume 1(Introduction; Abbreviations; The Open; ... 3Part I. The Tournaments 7 The Open Championship 9U.S. Open Championship 200• Volume 2 •Professional Golfers Association of America Championship 397Masters Tournament 518Part II. The Players, ... 94 88 18234T Anderson, William A. 96 87 18320 1891 / The Open Championship PART I. THE TOURNAMENTSPos Player A R1 R2 R3 R4 Tot P/M WThis page intentionally left blankTABLE OF CONTENTS•...
... ADVANCING THE HEALTH, SAFETY, AND WELL-BEING OF OUR PEOPLE The Department of Health and Human Services enhances the health and well-being of Americans by providing for effective health and human ... and security, standards and interoperability, public health, research and evaluation, technical assistance and funding for the National Institute of Standards and Technology. The HITECH ... Aging…………………………………………………………………… 86 Office ofthe Secretary General Departmental Management………… ……………………………………… 89 Office of Medicare Hearings and Appeals………………………… …………………. 91 Office ofthe National Coordinator...
... Maryland and federal law regulate the use of ―child labor‖ (i.e., the employment of persons below the age of eighteen) andthe employment of people to do work in their homes. The Maryland Division ... but the business goals ofthe venture may be accomplished in any of these formats. III. MERGERS UNDER MARYLAND LAW One last example ofthe flexible options offered by Maryland law is in the ... Division, Office ofthe Attorney General David M. Lyon, Principal Counsel, Office ofthe Attorney General, Department of Assessments and Taxation Craig A. Nielsen, Principal Counsel, Office of the...
... interestingly, in contrast with the lack of other components ofthe peroxisomal proteinimport machinery, the deficiency of Pex4p does notinterfere with the assembly ofthe components of the importomer. In ... acontrol. The corresponding solubilisates(right panel) were analyzed for the presence of Pex1p and Pex6p as indicated. The defici-ency of either protein ofthe AAA peroxincouple in the absence of ... cytosol. The specifity of the precipitation is confirmed by the lack of detection of the abundant proteins Fbp1p and thiolase in the eluates (data not shown). Figure 1B shows a compar-ison ofthe cytosolic...