... representative ofthediversityof P1 with a divergence up to 17.8% were selected These isolates are representative ofthediversityofthe other parts ofthe genome as phylogenetic reconstruction from the ... accumulation of gus-si-RNA and accumulation of mRNA in the del area, and an absence of gusmRNA in the dis area (Figure 6B, lanes 6–7) Then, for the same samples, we investigated the presence of P1 in the ... host we highlighted the functional diversityof RYMV isolates on silencing suppression The functional diversity at the scale of virion is not correlated to geneticdiversityofthe P1 protein suppressor...
... Overview of research methods, evaluation on geneticdiversityand identification molecular maker in Dendrobium species 1.3.1 The concept ofgeneticdiversityGeneticdiversity is thediversityofthe ... identify thediversity in the gene level, provide the basis for assessing the conservation value of species and populations Therefore, the creation of a database of DNA (DNA fingerprinting) ofthe ... study ofgeneticdiversity Biodiversity is essential for the survival ofthe species, natural communities and very important for humans 1.3.3 The method of evaluation on geneticdiversityand indentification...
... environment and transmission to the community The present study was undertaken with the aim of determining epidemiologyof clinical and carrier staphylococci andmolecular studies of their acquisition and ... study AA and MAK coordinated and participated in design ofthe study MR and KHB participated in the design and coordination and helped in the draft ofthe manuscript MJD and Page 13 of 15 (page ... all the microbiological, molecularand clinical laboratory experiments as a part of his PhD thesis and has written the draft manuscript MAT and JAK provided strains and helped in the design of the...
... under the best-fit GTR+I+Γ model of evolution, based on the L-genomic segment of SWSV and other well-characterized hantaviruses The phylogenetic positions of SWSV variants from Finland and Hungary ... performed moleculargenetic studies and sequence and phylogenetic analyses Preliminary data were provided by SA and JWS AGH and JAC provided tissues and carried out themolecular identification of wild-caught ... Northern Europe, including Scandinavia and Great Britain (but excluding Ireland), and across Russia (Fig 1), provided an opportunity to investigate thegeneticdiversityand phylogeography of...
... Program ofthe Ministry of Science and Technology of China (2006CB101902), the '863' Program ofthe Ministry of Science and Technology of China (20060102Z1097 and 2006AA10Z185), andthe National ... the Xcc strains tested The wxc genes are involved in the biosynthesis ofthe LPS O-antigen, which is the most variable portion of LPS [19,68] Thediversityofthe wxc cluster indicates that the ... display ofthe aCGH clustering result ofthe two sequenced genomes ofthe Xcc strains 8004 and ATCC33913 Each row corresponds to the specific ORFs on the array andthe ORFs are arranged in the genome...
... : The comparison ofthe model with the predictor ofthe Karakiewicz nomogram only with the one with predicators of both the Kattan nomogram andthe Karakiewicz nomogram b : The comparison ofthe ... model with the predictor ofthe Kattan nomogram only with the one with predicators of both the Kattan nomogram andthe Karakiewicz nomogram c : The proportion ofthe variation explained by the Kattan ... a : The comparison ofthe model with the predictor ofthe Karakiewicz nomogram only with the one with predicators of both the Leibovich prognostic score andthe Karakiewicz nomogram b : The comparison...
... with the glutelin fraction, which showed a high degree of polymorphism, finding up to 32 polymorphic bands in all the trees evaluated On the other hand, the understanding ofthegeneticdiversity ... 0.283) The 27.0% ofthegeneticdiversityof this last group was detected among populations; however, this value was higher in Cordoba with 34.8% of total geneticdiversityThe proportion ofgenetic ... population genetics, breeding andgenetic resources Suderland, Sinauer Associates Inc Publishers: 64–82 HUANG H., DANNE F., KUBISIAK T.L., 1998 Allozyme and RAPD analysis ofthegeneticdiversity and...
... While there have been larger, more detailed studies reported in the medical literature, this is the first such report from the UAE and therefore we not know whether our experience is typical ofthe ... MH Participated in themolecular analysis andthe antimicrobial susceptibility testing RA participated in the coordination ofthe microbiological and biochemical analysis Page of (page number not ... person spread We report the clinical characteristics of bacteraemia in Tawam Hospital, UAE and investigate themolecularepidemiologyof S maltophilia bacteraemia isolates from the hospital aiming...
... took place in the USA, Japan, and other countries in the 1970s (subgenogroup B1), in Hong Kong, Australia, andthe USA in the 1980s (subgenogroups B1, B2, and C1), and especially in the Asian Pacific ... than 93% ofthe nucleotide identity between the C2-like isolates andthe reference strains, each gene region of these two isolates was further sequenced and recombination analyses conducted The nucleotide ... changing of subgenogroups, andthe potential of severe and fatal outbreaks on their way, it is a must to monitor the recombination events as well as antigenic andgenetic evolution of EV-71 very...
... or JPY), Tennessee (TN), or the Netherlands (NL) and 16 AMPV F sequences The first two digits ofthe HMPV sequence names indicate the year ofthe isolate The names ofthe AMPV sequences indicate ... Japan, andthe Netherlands Our data confirm that there are four distinct genetic lineages of HMPV, provisionally designated as A1, A2, B1 and B2 [32] These data further show that these genetic ... strains of HMPV and assigned to one ofthe four proposed lineages (A1, A2, B1, or B2) based on phylogenetic analysis, discussed further below [32] Ofthe 68 strains sequenced, 34 (50%) were of the...
... interpreted the data, and wrote the manuscript MS, RRG, and AK helped design the study, performed detailed phylogenetic analysis ofthe sequences, and helped write the manuscript SHQ and HJ together ... intervals for the origin of HTLV-2 as it migrated out of Africa and Asia and into the Americas via the Bering land bridge and 40,000 – 60,000 ya as confidence intervals for the origin of HTLV-1 in ... outside thediversityof all other PTLVs The observed low nucleotide substitution rate, absence of evident genetic recombination, and conserved genomic structure of HTLV-4 demonstrate the genetic...
... linked mother-infant pairs The degree ofgenetic variability ofthe env gp41 sequences from five mother-infant pairs was determined on the basis of pairwise comparison ofthe nucleotide and deduced ... sets, the levels ofgeneticdiversity ranged from 0.01 to 0.03 when estimated by both Watterson and Coalesce methods The HIV- 1 populations found in the mothers displayed overall same geneticdiversity ... Length of infection: The closest time of infection that we could document was the first positive HIV- 1 serology date or the first visit ofthe patient to the AIDS treatment center where all the HIV- 1...
... originated from the evolutionary process ofthegenetic code as suggested by the GNC-SNS primitive genetic code hypothesis The discussion on the robustness ofthegenetic code is consistent with the results ... composed of 64 codons and 20 amino acids (see Table 2) Origin oftheGenetic Code (GNC-SNS primitive genetic code hypothesis) Our studies on the origin ofthegenetic code were initiated from the ... 2002) The GC-NSF(a) hypothesis on creation ofthe first family genes under the universal genetic code led us propose subsequent theory on the origin ofthegenetic code as GNC-SNS primitive genetic...
... necessary for the establishment of a zoonotic virus The host must be susceptible to the virus, the environmental conditions must provide stability and viability ofthe virus andthe host, andthe virus ... to the development of a disease In the past, the detection and characterisation of novel viruses on the base ofgenetic information, was impossible However, the development of deep sequencing and ... Ethiopia in the east and down to South Africa in the south (Fig 1) Colonies have also been recorded on several off-shore islands in the Gulf of Guinea, Zanzibar, Pemba and Mafia, on the Arabian...
... all the NMR and force field data The quality ofthe ensemble of protein structures can be evaluated by the number andthe size of constraint violations, the total potential energy as well as the ... on the refolding of α-Lactalbumin showed that the intermediate, often called the burst-phase intermediate, accumulated quite quickly at the beginning ofthe refolding within the dead time ofthe ... Furthermore, due to the bumpiness ofthe landscape, protein molecules can be trapped and accumulated at local minima before they finally fall into theglobal minimum, so folding intermediates and...
... samples, pairs and randomly cloned DNA fragments with complementary sequences with the sequence ofthe samples The RAPD markers used in the study ofdiversityandgenetic mapping ofthe typical ... ZG and MTD65 The above information is very useful and can be applied in breed selection and creation The structure ofthe thesis The thesis comprises of 115 pages, which are divided into the ... SSR molecular indicator The correlation between RAPD and SSR is in the analysis of soybean To compare the correlation ofthe two molecular indicators RAPD and SSR with the polymorphic analysis of...
... 1969) 1.2.4.7 Measure ofthe loss ofgeneticdiversity Measures ofthe loss ofgeneticdiversity can be derived from fe, fge, and Nenf The amount ofgeneticdiversity (GD) in the reference population ... thediversityofthe whole set of breeds and H(S\K) is the heterozygosity ofthe set without breed K The aggregate diversity D is the sum of both components (i.e CB and CW) weighted by F ST and ... to the members of Department of Animal Breeding and Genetics, Institute of Agricultural Sciences for Southern Vietnam andthe recent Institute of Animal Sciences of Southern Vietnam, Ministry of...
... shows the final example, a universally conserved component ofthe core ofthe signal recognition particle (SRP) The structure ofthe complex (PDB code: 1HQ1) andthe binding affinity of a series of ... on the presence or absence of a cytosine at position )5 in the RNA A likely explanation for the importance ofthe )5 cytosine mutation is offered by the observation that the amino group ofthe ... are located in a double-stranded region of RNA, and not interact with the protein [54], yet the disruption ofthe helix clearly affects the binding energy The effect of these changes in RNA conformation...
... constant value of Hz for the H1 signal ofthe GlcNAc residue andthe NMR chemical shifts of H5 and C5 ofthe rhamnose residues showed that the former is b-linked andthe latter are a-linked The NOESY ... the major O repeat ofthe OPS has structure 2: each other in the position of substitution of one ofthe rhamnose residues (RhaIV) in the main chain andthe site of attachment ofthe GlcNAc side ... either the LPS core structure or /and in the mode ofthe attachment ofthe OPS to the core Therefore, strains of pathovar porri are clearly distinct from other P syringae pathovars in serology of...
... into the pH dependence of their NMR parameters over the pH range 3–8 Obviously, the pH titration curve ofthe amide proton andthe titration curve of 31P of phosphorylated serine residue have the ... pKa1 and pKa2 The pKa value is determined from a fit ofthe phosphorus chemical shifts ofthe without ionic, monoionic and diionic forms ofthe phosphate group as a function of pH [33,34] The phosphorus ... neutral pH, affects the electrostatic potential and quite often the conformation ofthe modified protein Even in the absence of rearrangement, the change in the electric field and steric hindrance...