... (JASCO).Fig. 3. Alignment ofthe sequences ofthe second Kunitz-modules ofthe human and fugu WFIKKN proteins (wfikkn_hu_2; wfikkn_fugu_2) with the second Kunitz-domains ofthe human andthe two fugu WFIKKNRP ... [8–10]). Thermal unfolding ofthe proteinwas monitored at 203 nm at a heating rate of 60 Cặh)1.Effect ofthe recombinant protein on the activity of proteases The activity ofthe proteases on synthetic ... determinant ofthe hydrophobic SÂ2 bindingpocket of trypsins is the side-chain of Tyr151 [18]. The importance of this residue is underlined by the fact that in the case ofthe second Kunitz-domain of...
... 5 of 5 acetabulum and lobulated cyst in the soft tissue around the right hip region. Hydatid disease was considered as adiagnosis and Core biopsy ofthe abnormal region and aspiration ofthe ... organs. Due to their physiologic role as capillaryfilters and their vast capillary volume, the liver and lungare most often affected. The brain, the muscle s or the bones are the more frequently ... disease ofthe pelvis: a case report and review of the literature. Journal of Orthopaedic Surgery and Research 2010 5:17.Submit your next manuscript to BioMed Central and take full advantage of: ...
... Kulin, and Cranbrook. The mean levels of translucency of noodles made from the soft, low-FSV wheat flour andthe soft, high-FSV wheat flour (9.25% protein) were 39.75 and 43.75%, respectively. The ... each of the six samples was measured, the results ofthe 32 panelists were combined, andthe mean scores were compared with instrumental gloss and translucency values. Twenty-one ofthe 32 ... boiling time andthe degree of starch swelling (Crosbie 1992). Accurate measurement ofthe color of boiled noodles necessitates the use of a method that minimizes the effect of variation on...
... (3) the location ofthe impact andthe occur-rence ofthe tumor corresponded exactly one to the other; and (4) there was a more than one year interval between trauma andthe appearance ofthe ... interval between trauma andthe appearance ofthe tumor of at least 1 year, a longer latent period increasing the likelihood of a causal relationship. 5. The presence ofthe tumor must be proved ... reveal the severity and location ofthe traumatic brain injury andthe subsequent formation of a neoplasm at the same site, can provide convincing evidence for the traumatic causation of a brain...
... cultivation, and that ofthe d´eclass´e world of the theatre. Both exploit the potential of aesthetic experience to bypass the equation of class, work and personal limitation. Having taken the only ... to the University of Jena. But most of his copies The pursuit ofthe subject – the circumvention of consciousness releases the inherent sympathy of the higher, physical organs ofthe ... Schelling, and its literary counterparts, the classicism and Romanticism of Goethe,Schiller, Schlegel, Hardenberg-Novalis and others. At the end of that dialogue stands the system of perhaps the ultimate...
... how is such an image to greet the murmuring andthe crackling andthe tinkling andthe crying andthe sighing and rumbling andthe roaring of background noise? The question is rushing towards ... There was the affectivity that passed between birds and tree. There was the agitation, the vacillation, the vibration. There was, also, the movement ofthe time that made the time of day, the ... Serres, the song ofthe taut and tenuous agitation at the bottom ofthe world. -'There is background noise, then a noise in the midst of that back- ground noise, and suddenly there's the...
... slope ofthe observable change with the temperature; and, the last term is the effect of urea on the baselines. To allow for comparisons among the thermal-(see below) and chemical-denaturations, the ... (enzymeI ofthe PTS), the first protein in the cascade of proteinsforming the PTS, to HPr, the histidine-phosphocarrierprotein. HPr is the smallest protein in the protein cascade of the PTS and it ... concentrations, and the values ofthe DHmÂ, DSmÂ, Tm and DCp obtained by the approach of Pace and Laurents [39] (Table 2), a detailedanalysis ofthe dependencies of DHmÂ, DSm and DCpÂ...
... confirming that the mutation of Tyr186 to Phe186 does not grossly perturb the overall framework ofthe enzyme or the active site. The active sites of the enzymes are shown in stick format with the sigmaA ... and indicate re-oxidation ofthe flavin andthe formation ofthe hydride-Meisenheimercomplex of TNT. (C) As for panel B except the spectral changes are recorded over an extended time period and ... resolved and are boundbetween the progesterone andthe protein (Fig. 7A).In comparison to the oxidized structures, the FMNisoalloxazine ring is less planar, with both the N5atom andthe C8-C7...
... of ISP and the extramembranous cd2 helix of cytochrome b areimportant for maintaining the structure ofthe hinge region and, by consequence, the movement ofthe headgroup and the integrity of ... region andthe cytochrome bextramembranous cd2 helix is important for maintaining the structure ofthe hinge region and, by consequence, the movement ofthe headgroup andthe integrity ofthe enzyme.Keywords: ... below.Instability ofthe mutant enzymeFurther analysis ofthe kinetic parameters ofthe G167Emutant was hindered by rapid inhibition ofthe activity of the bc1complex during the assay, which...
... monophosphates and, in particu-lar, UMP and GMP. The structure of NagD has beendetermined andthe occurrence of NagD in the context of the nagBACD operon indicated its involvement in the recycling of cell ... arabinose as the sole carbon and energysource. In the presence of glucose and arabinose, the doubling time ofthe mutant (49.7 ± 0.3 and 52.4 ± 0.1 min, respectively) is comparable to that of the wild-type ... specificity or the lack thereof [10,13,20].Similar to the other members ofthe NagD family,AraL shares two Asp residues in the cap domain(Fig. 2). To date, the number of characterized mem-bers of this...