the exceptional measures were coherent with other eu initiatives undertaken in response to the 2005 06 ai crisis
Tài liệu Báo cáo khoa học: Comparison of a coq7 deletion mutant with other respiration-defective mutants in fission yeast doc
... Spcoq7-b, were used to amplify coq7, and the amplified fragment was then cloned into pT7Blue-T to yield pTPC7 The SalI–SmaI fragment containing coq7 was cloned into the SalI–SmaI site of pREP1 to yield ... measured using a YSI model 53 oxygen monitor Staining of mitochondria and fluorescence microscopy Mitochondria were stained with the mitochondria-specific dye, MitoTracker Red FM (Molecular Probes, Inc., ... constructed and used to disrupt the chromosomal cyc1 allele in the haploid wild-type PR110 strain The disruption was verified by PCR using appropriate primers To obtain the mutants in the same genetic...
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Báo cáo khoa học: Hepatocyte nuclear factor-4a interacts with other hepatocyte nuclear factors in regulating transthyretin gene expression pot
... changes in the interaction of HNF-4a with the other HNFs HNFs bind independently to the TTR promoter To identify the potential mechanism underlying the interaction of HNF-4a with the other HNFs in ... treatment with cytokines HepG2 cells were treated with or without cytokines for 18 h The interaction of HNF protein with the DNA binding site was determined by ChIP assays with antibodies against ... DNA–protein binding assays to detect whether HNFs affect each other s binding ability A biotinylated DNA probe encompassing the TTR promoter with WT or individually mutated HNF binding sites was incubated...
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Báo cáo hóa học: " Incidence of the V600K mutation among melanoma patients with BRAF mutations, and potential therapeutic response to the specific BRAF inhibitor PLX4032" potx
... shown to be positive for the BRAF V600E mutation The patient was treated and achieved an excellent clinical response to PLX4032, associated with substantial reduction in the tumor burden and pain ... those of others reported in the literature, indicate that the incidence of BRAF V600K mutations in melanoma patients appears to be higher than is commonly assumed It can be present in up to 30% ... RH, in charge of analyzing tumor specimens and participated in writing the manuscript All authors have read and approved the final manuscript Competing interests The authors declare that they...
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Tài liệu Báo cáo khoa học: Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress pdf
... The main finding of this study is the identification of a metabolic gene switch from fatty acids to glucose in the murine female heart at baseline linked to enhanced mitochondrial respiratory capacity ... MO] containing luciferin and luciferase The reaction of the luciferin ⁄ luciferase mixture, energy donor (ATP) and oxygen results in the emission of light The intensity of the bioluminescence ... (decrease in fatty acid utilization, increase in glucose utilization) in the murine female heart Moreover, our data suggest that these changes occur in an Akt-independent manner Further studies are therefore...
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Báo cáo khoa học: DNA-binding characteristics of the regulator SenR in response to phosphorylation by the sensor histidine autokinase SenS from Streptomyces reticuli doc
... ending at the 5¢-end of binding site III, and containing a deleted binding site II) To determine whether SenR had already been in a phosphorylated form within E coli, the obtained protein was incubated ... eluted with buffer W containing 2.5 mM desthiobiotin (lane 3) SenSCH199A was purified in the same manner (lane 4) To obtain SenR, a cytoplasmic protein extract (lane 5) containing SenR obtained ... DNA-binding characteristics Discussion The designed cloning procedures allowed us to obtain the cytoplasmic domain of SenS carrying a Strep-tag (SenSc) and the full-length SenR protein with a...
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Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress pot
... 3-fold increase in glucose and a more than 30-fold increase in lactate, whereas the levels in controls remained constant The arginine kinase gene (AK) and the eukaryotic translation initiation factor ... whereas the rest were located in trunks Two types of cells were observed: the majority of cells showed homogeneous CHH staining in the cytoplasm (Fig 4C) with a visibly large nucleus, whereas others ... during summer, in combination with the changes in salinity, are associated with high mortality in this species [25–27] In view of CHH involvement in response to stress in other crustacean species,...
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Báo cáo khoa học: Proteolysis of the tumour suppressor hDlg in response to osmotic stress is mediated by caspases and independent of phosphorylation pot
... dissociation from the cytoskeletal protein GKAP, therefore releasing it from the cytoskeleton into the cytoplasm [3] Our aim in this study was to gain a better understanding of the role of hDlg ... protein from cytoplasm and membrane fractions were immunoblotted using the antibodies indicated (C) CaCo-2 cells were preincubated with or without the kinase inhibitor BIRB0796 (1 lM) and in the ... recognizes total hDlg (anti-hDlg) (B) HeLa cells were preincubated for h with or without the pankinase inhibitor staurosporine at the concentrations indicated, and treated with 0.5 M sorbitol for 60 min,...
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Báo cáo khoa học: The calcium-binding domain of the stress protein SEP53 is required for survival in response to deoxycholic acid-mediated injury pdf
... transfection, cells were split and plated in media containing Geneticin to select for cell containing vector DNA After three weeks, the number of cells were determined by fixing cells and staining with dye ... lines to determine whether the gene product had protective effects from DCA damage in vitro Cellular stress protein responses play a key role in minimizing cell injury and maintaining tissue integrity ... (p53-null) were transfected with the indicated DNA vector (1 lg) and one day after transfection, cells were split and plated in media containing Geneticin to select for cell containing vector DNA (as in...
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Báo cáo khoa học: BRCA1 accumulates in the nucleus in response to hypoxia and TRAIL and enhances TRAIL-induced apoptosis in breast cancer cells pdf
... expected to increase We observed an increase in HIF-1a protein, and thus were confident that the cells were being maintained in a low-oxygen environment and were responsive to low oxygen There is ... epithelial cells, we sought to determine whether TRAIL was involved in the localization change First, we assessed whether there was a change in TRAIL expression in MDAMB-468 cells in response to ... TRAIL-mediated apoptosis in HCT116 human colon carcinoma cells by inhibiting the translocation of Bax from the cytosol to the mitochondria, thereby preventing the release of cytochrome c and progression...
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Báo cáo khoa học: Irreversible cross-linking of heme to the distal tryptophan of stromal ascorbate peroxidase in response to rapid inactivation by H2O2 ppt
... the APX apoprotein Identification of the heme-binding amino-acid residue in H2O2-inactivated stromal APX To determine which amino-acid residue in the apoprotein was cross-linked to heme, the inactivated ... of the apoprotein of the catalytic site ˚ (Fig 4B) Because the indole ring of Trp35 is 3.18 A from the porphyrin ring in tsAPX (the distance between nitrogen of the indole ring and C6 of the ... transfer of the radical in the reaction intermediate of tsAPX Theoretically, the propionate side chain is also involved in electron transfer from amino-acid residues to the porphyrin [24] The tendency...
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Báo cáo khoa học: Molecular identification and expression study of differentially regulated genes in the Pacific oyster Crassostrea gigas in response to pesticide exposure doc
... b-1,3-Glucan binding protein 3e-6 Procathepsin L 1e-12 Meningioma expressed antigen 7e-19 Lipoprotein receptor related protein 2e-24 Fucolectin-1 6e-4 Cytoskeleton Tubulin alpha chain 4e-40 Tubulin beta ... function in the respiratory chain, cell communication, the immune system, or the regulation of protein or the cytoskeleton Only a few are specific to xenobiotic detoxification One, the glutamine synthetase ... receptors and immune system b-1,3-Glucan binding protein 3e-6 Putative senescence-associated protein 5e-46 Cavortin 1e-5 Cytoskeleton Tubulin alpha chain 4e-17 Tubulin alpha chain 5e-20 Tubulin...
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Báo cáo hóa học: "Investigating the synchronization of hippocampal neural network in response to acute nicotine exposure" pdf
... substantial loss in nicotinic receptors in cortex and striatum According to the growing amount of findings, accumulation of amyloid plaques which are the baseline indicator in the brain for AD is interfered ... brain tissue were made using the vibratome followed by transverse sectioning to create tissue containing hippocampus and entorhinal cortex All Sectioning was done while the tissue was under the ... needed in these cases [24] In the current study, we have investigated the effect of nicotine on the complexity of the neurons and the activity of the gamma oscillations in the Schaffer CA1 cell line...
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báo cáo hóa học:" The hen model of human ovarian cancer develops anti-mesothelin autoantibodies in response to mesothelin expressing tumors" docx
... according to manufacturer’s instruction (Vector Laboratories, Burlingame, CA) Control staining consisted of replacing the primary antibody with PBS containing 1% BSA The HRP was reacted with diaminobenzidine ... surface staining was more intense than in the cytoplasm In addition to elevated expression of mesothelin in ovarian tumors, there was evidence of circulating mesothelin antibodies in hens with ovarian ... vaccine, our objective was to determine if the hen expresses mesothelin, if mesothelin expression is increased in ovarian tumors and if circulating mesothelin autoantibody is associated with...
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Báo cáo lâm nghiệp:"Growth and biomass partitioning of Fagus sylvatica L. and Quercus robur L. seedlings in response to shading and small changes in the R/FR-ratio of radiation" pot
... (in cm respectively)) and the dry mass of the main stem (DMshoot) at the end of the experiment, the dry mass of the main stem at the start of the experiment and after 50 days was estimated using ... On the other hand, in contrast to beech, height growth of oak seedlings in the first years is known to be highest in moderate shade [18, 55] As even the oak seedlings under the black nets were ... on forest reproductive material The seeds were sown into a permeable pot of 2000 cm3 in size and the seedlings stayed there to the end of the experiment The rooting substrate consisted of a mixture...
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Báo cáo khoa học: "The behavior of oaks in response to natural and induced exposure to the surfactant ABS" potx
... decreased in the following order: Q pubescens, Q frainetto, Q ilex and Q robur The same trees were sprayed again in 1991: initial data revealed structural degradation of the leaf waxes similar to that ... difference in tube length be- germination was containing no lower in tween the windward and leeward sides of exposed trees The addition of ABS to the culture reduced germination in all trees The extent ... species (5 for the spray treatment and controls) were maintained in a plastic chamber where, during each growing season, they were treated twice weekly for 10 weeks with 50 mg/l ABS in a fog-type...
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Báo cáo y học: "Predictive network modeling of the highresolution dynamic plant transcriptome in response to nitrate" pps
... NRT1.1 is induced at 20 minutes (compared to KCl controls, and in comparison to gene expression at time minutes) The timing of induction of other sentinel genes involved in the ‘primary NO3 response ... nitrate signaling with other hormone signals is likely to involve the genes regulated by nitrate after minutes This leads to the hypothesis that, from to minutes, the genomic reprogramming concerns ... gamma, the kinetic time constant (in minutes) and the parameter regularization coefficient lambda, based on the quality of fit to the training data (from to 15 minutes), as measured by the signal -to- noise...
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báo cáo khoa học: " The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism" potx
... targeting determinants for chloroplasts and mitochondria are usually located at the N-terminus of the protein; therefore, a fusion protein with GFP fused to the C-terminus of TSPO was made Using ... membrane of the mitochondria [6,20] However the localization of TSPO in plants remains controversial Lindenman et al [19] used immunogold staining to show that TSPO is localized in the outer membrane ... TSPO to the C-terminus of YFP resulted in YFP:TSPO being found in the endoplasmic reticulum and the Golgi stacks [23] The Arabidopsis genome contains a single TSPOrelated gene (AtTSPO) The predicted...
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báo cáo khoa học: "The grapevine guard cell-related VvMYB60 transcription factor is involved in the regulation of stomatal activity and is differentially expressed in response to ABA and osmotic stress" ppt
... discriminate between the Arabidopsis and grape MYB60 proteins and other closely related proteins from subgroup 1: a distinctive PHEEG signature in the MYB domain, located in the loop connecting the ... closure of the pore, in response to internal signals and environmental cues, allows the plant to cope with the conflicting needs of ensuring adequate uptake of CO2 for photosynthesis and preventing ... pVvMYB60:GUS seedling (E) Magnification of leaf primordia in (D), showing staining of differentiating stomata (F) Detail of a pVvMYB60:GUS mature leaf, showing staining of fully differentiated stomata (G)...
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báo cáo khoa học: " Comparison of the transcriptomes of American chestnut (Castanea dentata) and Chinese chestnut (Castanea mollissima) in response to the chestnut blight infection" doc
... processes DNA or RNA binding hydrolase activity kinase activity nucleic acid binding nucleotide binding other binding other enzyme activity other molecular function protein binding structural molecule ... processes DNA or RNA binding hydrolase activity kinase activity nucleic acid binding nucleotide binding other binding other enzyme activity other molecular function protein binding structural molecule ... processes DNA or RNA binding hydrolase activity kinase activity nucleic acid binding nucleotide binding other binding other enzyme activity other molecular function protein binding structural molecule...
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báo cáo khoa học: " PR genes of apple: identification and expression in response to elicitors and inoculation with Erwinia amylovora" doc
... method were collected at each time point Elicitor treatment Elicitors were sprayed to run-off using a hand-pumped atomizing sprayer Elicitors were diluted in water and were applied to hours into the ... young growing shoots, through the activity of insects, wind-driven rain or hail The second method, slicing the young leaf lamina on both sides of the mid-vein, was used to try to maximize the number ... parallel to the midvein with scissors contaminated with bacteria The third inoculation method, snipping off the distal approximately 1/3 of the young leaf with contaminated scissors, proved to be the...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose