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Báo cáo khoa học: The bioactive dipeptide anserine is transported by human proton-coupled peptide transporters doc
... see[11,12]). At the renal epithelium, small peptides arereabsorbed from the glomerular filtrate into the cells by the subtypes PEPT1 and PEPT2. PEPT2 is alsoexpressed in other tissues, such as ... as the differencein the currents measured in the presence and absence ofsubstrate.AcknowledgementsThis study was supported by Deutsche Forschungs-gemeinschaft grant BR 2430 ⁄ 2-1 and by the ... at the intestinal epithelium. The purpose of this study was to investigate whether anserine is a substrateof the H+⁄ peptide cotransporters 1 and 2 (PEPT1 and PEPT2). We firstassessed the...
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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
... Kmvalues is exclu-sively displayed by the tetramer forms, and low-efficiency kinetics with high Kmvalues is displayed by the dimer forms. These observations strengthen the previous hypothesis that ... lgặmL)1indicate thatdissociation of the tetramer to the dimer is a slowlyprogressing process. This is supported by the kineticbehaviour of the recombinant enzyme as shown inFig. 5D, where the enzyme ... kDa (see Table 1). Therefore, it can be concludedthat the nature of the base is insignificant for the tetra-merization effect. The next goal was to determine the role played by the sugar and phosphate...
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Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx
... proteinswithin the cell. We found this translocation to bedependent on the final amino acids of the receptorprotein. Similar to the other PDZ domain interactionsthat have been shown to be abolished by ... signal is impaired.We finally examined the effect of deletion of the components of the PDZ binding motif on the signal-ing properties of the receptor in Ca2+imagingexperiments. Truncation of the ... summary, when the interaction betweenMUPP1 and OR2AG1 is inhibited by over-expressing the interacting PDZ domains, the resulting response ofOR2AG1 to odorant is modified in that the rapiddecay...
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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf
... which is likely to pre-dispose to an irregular rejoining. Given high fluidityand self-organization, one speculation is that the struc-tural essence of the nuclear foci could be illustrated by the ... synapsis of the complexes promotes the juxtaposition between the opposing DNA ends,prior to their processing and ligation [44]. However,this scheme cannot fully explain the evidence for the in ... addition to the nuc-leosome component H2AX, the change in the phosphorylated state of histone H1 would regulate the aggregative nature of the array of nucleosomes.Concerning DNA-PKcs, the results...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
... fgl2 mRNA. The left side of the figure is the sequence ladderusing the same primer and the pM166 clone astemplate. Arrow indicates the nucleotide that is matched to the band present in the primerextension ... sufficientfor the basal expression of the fgl2 gene. This finding is consistent with the observation that fgl2 is constitutivelyexpressed in cultured endothelial cells, as well as in the primary endothelial ... in some cells and tissues is known to play this role, suchas in the CNS [9]. The biological importance of constitutivefgl2 expression is exemplified by the observation that the fgl2–/–mouse...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... or even two residues, but is most likely accomplished by the concerted effects of a combination of key residues.Further analysis of these and other mutant SODs is currently underway.ACKNOWLEDGEMENTSWe ... Fentonreaction with the iron metal at the active site. The presenceof iron is therefore a prerequisite for its effect and suggeststhat the absence of reactivity with the MnSOD mutants is due to the lack ... identified to distinguish between FeSOD andMnSOD [7]. The position of the glutamine residue, which is structurally equivalent in the enzymes, is contributed by the N domain of FeSODs (Q69) and the C...
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
... egg extract. The mature eggs of m ost vertebrates stay at the m etaphaseof the second meiotic division until they meet sperm. In thatphase, the nuclear envelope is dispersed in the cytop lasm ... that the binding of the RS region to chromatinDNA is superior to the binding of the NM region to the chromatin p rotein because the binding mode of NK,which contains the NM and RS regions, is ... 4nM(data not shown). These resultsindicate that the increase in the affinity of NK to chromatin is an ATP-dependent reaction, and is caused by a kinase(s)in the c ytosol. Then, authentic p rotein...
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Báo cáo khoa học: The autophagic response to nutrient deprivation in the hl-1 cardiac myocyte is modulated by Bcl-2 and sarco⁄endoplasmic reticulum calcium stores ppt
... the autophago-some. The machinery controlling formation of the autophagosome involves two ubiquitin-like conjuga-tion systems. The first is the conjugation of Atg12 toAtg5 [15]. The other is ... GFP–LC3-positivepunctae. This result was surprising, as the upregulationof autophagy by starvation has been reported in vivo[19], in isolated primary cells [33], and in other celllines [52] [14,20]. This discrepancy ... in HeLa cells is bound toVps34. These diverging reports raise the possibility thatrheostatic control of autophagy by Beclin 1–Bcl)2inter-action is not a universal mechanism. F urther experimentsare...
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Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx
... while the fragments amplified by the Fig. 4. The transcription of a-F1-ATPase is controlled by a cassette containing the GAGA and Adf-1 elements. (A) Sequence of the )146/+94(relative to the main ... as established in this study. The relative promoter activities of the constructs measured in the luciferase assay are indicatedon the right by black boxes. The luciferase activity of the vector ... elementscritical for the activation of the a-F1-ATPase promoter.Computer analysis revealed the presence of two DNAsequence motifs in this region potentially recognized by the GAGA factor (GAF) and the alcohol...
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Báo cáo khoa học: Androgen receptor function is modulated by the tissue-specific AR45 variant ppt
... recruitment, due to the absence of the NTD. This might be overcome by the overexpression of cofactors. To test this hypothesis, weanalyzed the effects of two AR cofactors known tointeract with the AR LBD. ... observed. The important role of the NTD, which is absent in AR45, has been documented by severalstudies [40–42]. The hormone-dependent interactionbetween this region and the LBD of the AR is essen-tial ... primer specic for the extreme 5Â region wasthen used together with a primer recognizing the 3Â endof the AR coding part to amplify the complete codingregion of this novel AR form. The deduced amino...
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Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx
... mutagenesis of the N-terminus of the V1aR; this revealed that a single residue(Arg46) located in the distal segment of this domain is critical for high affinity agonist binding but not antag-onist ... than the wildtypeV1aR but once the agonist bound, the receptor was morelikely to signal. This dual role suggests that binding of the agonist releases the Arg46-mediated constraint on the ground ... rescued by a chimeric construct in which the N-terminus of the OTR is replaced with the corresponding sequence of the V1aR [10]. The role of the N-terminus has beenaddressed recently by alanine-scanning...
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
... that the desABCD cluster is expressed as a polycistronictranscript. Expression of the four genes is coordinatelyregulated by iron limitation, as shown by the RT-PCRanalysis, and there is overlapping ... and its regulation is probably mediated by a cascade mechanism, ratherthan by direct interaction of DmdR1.On the other hand, the viuB gene (SCO2781) wasnot transcribed under the conditions tested ... con-tains the repressor-binding site, is boxed, and the ribosome-binding site is underlined. The ATG is shown in bold letters. The reported tran-scription start site of the S. pilosus desA promoter is...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008