... wrote the first draft ofthe manuscript JJ, JC and JMA contributed to the final version ofthe manuscript GA, RB-D and AR helped inthe data analysis and interpretation JJ and JC supervised the ... changes inthe pattern of chemokine gene expressionin circulating leukocytes The data suggest that PPARα activators may be useful inthe attempt to decrease the risk of atherosclerosis and may ... significant effect of fenofibrate inthe MCP-1/CCL2 gene expression However, there was a significant decrease inthe gene expressionofthe CX3CL1/ Fractalkine in patients treated with fenofibrate with...
... matures inthe cytoplasm The synthesis and maturation of HCV is presumed to occur inthe cytoplasm This, in our opinion, is an evolving concept We have seen HCV particles inthe nucleus, inthe perinuclear ... reported inthe brain environment [25-27] These observations may partly explain the diminished cognitive function, depression and fatigue in these individuals The presence of HIV1 and HCV inthe same ... were seen inthe cytoplasm andinthe vicinity of budding HHV-6A particles (Figure 7B), and other incomplete HCV particles were also seen inthe perinuclear space (Figures 7A and 7D) These HCV...
... matures inthe cytoplasm The synthesis and maturation of HCV is presumed to occur inthe cytoplasm This, in our opinion, is an evolving concept We have seen HCV particles inthe nucleus, inthe perinuclear ... reported inthe brain environment [25-27] These observations may partly explain the diminished cognitive function, depression and fatigue in these individuals The presence of HIV1 and HCV inthe same ... were seen inthe cytoplasm andinthe vicinity of budding HHV-6A particles (Figure 7B), and other incomplete HCV particles were also seen inthe perinuclear space (Figures 7A and 7D) These HCV...
... including the lungs [8,14] Our data may explain this rapid and efficient dissemination of SARS-CoV By slowing down expressionof IFNs and their antiviral genesinthe infected tissue cells, the ... both the Caco-2 cells andthe low passage HEK 293 cells as useful systems for studying the influence of SARS- CoV on the immune system-independent induction of cytokines Interferon genesand their ... studies andthe RT-PCR analyses, participated inthe design ofthe study, and has given final approval ofthe version to be published FW carried out virus infections, participated inthe design of the...
... studied Ofthe seven genes examined in this study, the levels ofexpressionof IFN-g, IL-2 and IL12P40 genesinthe bursa tissues following H strain infection were increased compared with the IL-4, ... expressionof IL-5 mRNA inthe bursa of birds infected with the H strain increased continuously, peaking at dpi with a 7.47-fold increase (P = 0.00002) (Figure 3C) Theexpressionofthe IL-4 gene inthe ... examining the transcriptional profile of cytokines inthe bursal tissues of chickens infected with either vvIBDV H strain or the cell-adapted virus Ts strain at 1, and days postinfection (dpi) and...
... rain forest, the massacre of Father Nery Lito Satur and several others inthe Philippines, andthe public hanging of Ken Saro-Wiwa and eight other members ofthe Movement for the Survival ofthe ... waste dumping, natural resource exploitation, andthe consequent degradation ofthe means of subsistence of indigenous people The roles ofthe state and MNCs in suppressing the rights of communal ... policies and official behavior toward the non-core countries Basically, institutionalized discrimination refers to the policies ofthe dominant institutions inthe core andthe behavior of individuals...
... role ofthe N-terminal part ofthe kinase, containing a PAS domain, was established in signal transduction between the RH andthe kinase [6,7] Addition of H2 to HupUV before or during the incubation ... inactive [26] Comparison of HupSL regulations andthe functional roles of HupTUV in other T roseopersicina strains would provide further insight into the understanding ofthe loss of HupSL hydrogenase ... suggest that the transcript level ofthe hupTUV genes is below the detection limit or is missing in T roseopersicina Mutagenesis and homologous expressionofthe hupT and hupTUV genes In- frame deletion...
... constructs in cell lines or transgenic mice offers the advantage of determining the function of regulatory elements after integration into the genome and assimilation into chromatin i p j Laybourn and ... probes to genes whose expression is not expected to vary inthe experiment (in yeast, probes complementary to thegenes encoding actin andthe TATAbinding protein have been used) or spots of total ... expressed in COS cells shows incomplete protein processing as reflected by a smear of bands on the gel as a consequence ofthe overwhelming amount of plasmid DNA and subsequent protein present in the...
... testing of these models [reviewed in 58] and further broad comparative studies, including normal and aberrant flowers in a range of species, will aid understanding ofthe mechanisms underlying the ... Page of 15 Figure Expression profiles of Actinidia flowering genesin mature plant organs Real-time RT-PCR analysis ofthe Actinidia flowering genesinthe root, stem internode, leaf, flower and ... protein is required for sepal and petal identity On the other hand, the role of euAP1 genesin specification of sepal and petal identity inplants other than Arabidopsis is unclear andthe concept...
... levels of proinflammatory cytokines including IL-1β, IL-6, IL-8 and TNFα [12] LPS is a cell wall component of Gram-negative bacteria, and is the main endotoxin implicated inthe initiation ofthe ... for the conception and design ofthe present review, as well as the intellectual content, drafting and revision ofthe manuscript TMH and JAB also contributed significantly to the design ofthe ... cardiomyocyte differs from that ofthe adult and may lead to differences inthe cardiac response to sepsis and inflammation In addition to underlying differences inthe structure ofthe neonatal cardiomyocyte,...
... throughout the production, finishing and processing system; and welfare factors, both ofthe animals in terms of exhibiting natural behaviors and being free of disease, suffering, and mortality, andof ... research, as the training population grows in terms of numbers of genotyped animals, and density of SNP genotypes per animal Phenotyping is now the principal limitation in expanding the series of traits ... measure in most processing plantsInthe US, carcass marbling has been used as a surrogate for tenderness/eating quality More recently, QTL inthe region ofthe calpain and calpastatin genes have...
... expressionand inhibit expressionof intron-containing genes (Ruvolo et al., 1998) In contrast to the majority of cellular genes, many EBV genes expressed during lytic cycle are intronless, and ... defined as that portion ofthe pharynx which lies behind the nasal fossae and extends inferiorly as far as the level ofthe soft plate Nasopharyngeal carcinoma usually originates inthe fossa of ... cause methylation of some promoters of cellular genes, including the promoters of tumour suppressor genes, and results in down-regulation oftheexpressionof these tumour suppressor genes 1.2.5.1.2...
... to understanding the role ofthe AP2like gene inthe spike morphology of barley and wheat andin hybrids obtained from their crossing and for modification oftheexpressionof AP2-like genes to ... parental The chromosome substitutions 1D/1Hch and 2D/2Hch influence theexpressionofthe barley AP2 in tritordeum The results are of interest in understanding the role ofthe AP2-like gene inthe ... Alignments ofthe sequenced cDNA and DNA ofthe H chilense lines H11 and H208, and H vulgare line H106 have shown that the internal structure of exons and introns ofthe AP2-like gene described in this...
... al depends in part on the homology between the donor pathogen protein andthe natural physiological protein present inthe receiver, both inthe amino acid sequence ofthe protein andin its tridimensional ... (h), and intestinal epithelium (ie) (M, insert) ofthe posterior intestine (pi) Inthe CNS, the labeled divisions are the telencephalon (t), mesencephalon (m) including the optic tectum (ot), the ... part ofthe intestine (Fig 5M,Q,R) This last finding should be of interest because in terms of function, the fish intestine is highly regionalized both inthe larva andinthe adult [38] The posterior...
... on the right side ofthe figure The transmembranous domains ofthe protein at the N- and C-terminus are shaded The five apyrase conserved regions (ACRs) are underlined andin bold The 10 cysteine ... inthe apical membranes ofthe oxyntic-peptic cells [37] The distribution ofthe ectoATPDase on these epithelial cells is distinctly different from the other ATPDase inthe E-ATPase family, the ... suramin, the former activates while the latter inhibits the chicken smooth muscle ecto-ATPase [23,28] On the other hand, it was inhibited by high concentrations of azide, an inhibitor of CD39 and...
... and 3¢-UTR The major differences were a 26 bp insertion inthe 5¢-UTR ofthe cDNA and an insertion of 12 repeats with a consensus of CCAATGATGATCCAAGAAATCCACACTACAG (31 bp) inthe 3¢-UTR ofthe ... 32739 Li R, Hartley L & Robb L (2001) Cloning of rat interleukin 11 and interleukin 11 receptor alpha chain and analysis of their expressionin rat uterus inthe periimplantation period Reproduction ... AJ535687) and amino acid (lower line) sequence are numbered on the left according to the submitted sequences The 5¢- and 3¢-flanking and intron sequences are in lowercase Identical nucleotides in the...
... different cDNAs coding for the subunits A and B of V-ATPase The intracellular localization of these isoforms andthe combination ofthe isoforms is yet to be determined We are now trying to express ... divergent in group and proteins The results we obtained indicate that these two domains may play a critical role in complementability Thegenes AACEVAPD1 and were constructed in another yeast expression ... ratio ofthe three proteins could not be estimated Forgac described in a recent minireview [21] that the VO domain consisted of six copies ofthe c/c0 subunits and single copies ofthe other subunits...
... present inthe other members ofthe APP superfamily, such as the absence ofthe exon encoding the KPI domain andthe lack of a second heparin-binding domain [3,4,13,26] Comparative analysis ofthe ... number of regions even more conserved (Fig 1) All 12 cysteine residues inthe aminoterminal part of APLP2 are present in X-APLP2 The zinc-binding domain consensus sequence (GxExVCCP [13]) inthe ... proteins are 91% and 100% identical, respectively, including the GYENPTY sequence that is present in all APP superfamily members and is involved inthe intracellular routing ofthe proteins [17]...
... produced inthe skin The level of 7-DHP production and its hypothetical conversion into other metabolites (including 17-, 20-, 21- and 11-hydroxy-7DHP) are the subject of investigations in our ... that thegenesand proteins required for the P450scc system are expressed concomitantly inthe skin and skin cells Moreover, using an array of methods including chemical synthesis with TLC and ... parameters for the 7-DHC; the retention time for its ion with m/z 385.3 and UV spectra are shown inthe inset in (B) andin inset in (D) These results are in agreement with recent findings of Thiboutot...
... andthe corresponding gene has been cloned from human [23] Inthe present study we have cloned the murine genes coding for the enzymes involved inthe salvage pathway of GDP-L-fucose L-fucokinase ... variants ofthe murine fucokinase genes were expressed in COS-7 cells in frame with a 10-amino acid E2Tag present inthe pQM vector The molecular masses of fucokinase proteins were determined by ... human fucokinase cDNA is similar to the long splice variant of mouse fucokinase cDNA at the 3¢ splice region The first and third methionines inthe murine sequence, inthe upstream end ofthe CDS,...