... removed from the culture tube After thorough washing ofthe roots in tap water, the plantlets were transferred to sterile sandy soil at the ratio of 1:1 (garden soil: sand) into earthen pots and kept ... 0.5, 1.0 and 1.5 mg/L of IAA, IBA and NAA alone or in combination for induction of rooting In case of rooting experiment, 10 replicates per treatment were taken andthe experiment was repeated ... production of multiple shoots and average number of shoots/explant was presented in Table III The interaction of two cytokinins with low concentration of auxin enhancing shoot multiplication has been...
... OA and RA SF samples The ratio of staining intensities for the ~200+-kDa bands as compared withthe ~170-kDa bands was significantly greater inthe 10 RA than inthe OA samples (22.6 ± 35.0 and ... containing 100 mM β-mercaptoethanol and 2% SDS, then washed in TBST and reblocked with blotto One was stained with mAb A2C2 diluted in blotto (panel B), whereas the other was incubated in blotto ... inthe N-terminal heparin-binding domain, followed by iodinated secondary antibodies Withthe exception of RA SF samples 16 and 19, for which staining was restricted mainly to an ~200+-kDa band,...
... most ofthe middle and 4132 all ofthe C-terminal domains, including the postulated Hsp90-binding and dimerization regions [16] Mutants with truncations extending into the N-terminal domain, Cdc37(1–155), ... Cdc37 mine the identity and location ofthe nucleotide insertion Four mutants obtained contained in- frame insertions resulting inthe insertion of five amino acids into the Cdc37 protein Stop ... associated withthe client Cdk4 in NIH-3T3 cells [6] A complex consisting ofthe interacting domains of yeast Hsp90 and human Cdc37 has been crystallised and its structure determined [16] Amino...
... training and support As a part ofthe intervention, patients inthe intervention group were transferred for medical reasons only and remained on the orthopaedic ward until they had attained an ... here and accomplished significant changes in two out of three outcomes The continuity of caregivers and care content was maintained simply by eliminating transfers for other than medical reasons ... thestudy at admission and informed consent was obtained from each patient Participants inthestudy were only required to approve the use of their pre-fracture and clinical data Thestudy was...
... engaging in collaborative talk during content reading, they assisted one another in understanding the meaning of challenging words, getting the main ideas, and answering questions about what they ... knowledge of language use and they are quite aware of using idioms Moreover, they are at the first stage of training and gaining knowledge of culture andthe differences among cultures Therefore, the ... FINDINGS In this chapter, the author ofthestudy will deal withthe description ofthe survey and data analysis and findings Description ofthe survey: With a view to having the real picture of how...
... to be and remain the One ofthe Many, their “That-by-which” The Wang Bi Laozi therefore deals withthe problems of ontology, the relationship of Being and Entity, the One andthe Many, and Negativity ... question ofthe ruler’s stepping down and actually being “lowly,” “orphaned,” “solitary,” as there is ofthe Dao relinquishing its role asthe origin and support ofthe ten thousand kinds of entities ... me explain It is easy to see how the two lines express in some manner the political and policy insights of Wang’s Laozi Here and there the Laozi speaks ofthe Sage as practicing the teaching that...
... the hemoglobin release The releasing curves of pravastatin show release phases, the first is rapid release phase andthe second is slow sustained release phase with hemoglobin release similar ... sample[15] The release ofdrug was studied also in plasma andin PBS after addition of glutaraldehyde Pravastatin assay by UPLC A reversed phase UPLC method was developed and used throughout thestudy ... 226 Loading efficiency at 25oC The effect of concentration and incubation time on the percent ofdrug loading is shown in figures and at 25ºC and at 37ºC The percent is ofdrug loading was started...
... of Fe and Cu content into spherical and soot aggregates ofthe fine fraction indicates a combination of natural and anthropogenic sources influenced by smelter and incineration emissions inthe ... during the rain season (May-June) and from 31 to 38% during the dry season (January-February) due to the effects of soil resuspension and land erosion which contributes to an increase on the ... nm was measured [20] The results are expressed as MPO units One unit (U) was defined asthe quantity of enzyme necessary to catalyze an increase of 0.1 inthe absorbance at 655 nm and 25°C The...
... proliferation of each drug separately, andin combination with other drugs [23,24] The combination with no drug (Mix 1, Table 2) served as control, and was tested an extra five times to assess the standard ... proliferation in our study, any effect was certainly smaller than the effects ofthe other drugs investigated Testing several drugs simultaneously, as we did in this study, has the advantage that one can ... the experiments and drafted the manuscript JK participated inthe design ofthe study, performed the statistical analysis and helped to draft the manuscript PWL & NM conceived ofthe study, and...
... biomechanically compared in this study Clinic stability ofthe spine has been described as prevention of spinal displacement and damage to the nerves root and spinal cord from surgical trauma and other ... between the angle and moment Therefore, stiffness was considered as constant in this range of movement The slope ofthe least square fit line ofthe displacement-force curve yielded the same ... ofthe data, the software (Statistica'99; StatSoft, USA) was used andthe classification was performed with applying the Euclidian distance The Euclidian distance provided four main clusters (Fig...
... immunofluorescence and 3,3-diaminobenzidine disclosure) for aggrecan andthe staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of ... the type X collagen and Alizarin red staining, and participated in interpretation ofthe data AJF participated in interpretation ofthe data and contributed to the preparation ofthe final manuscript ... ofthe injected cells was also observed week following injection, andthe intensity ofthe matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen was...
... for 30 min), with or without 30-min preincubation with 10-6M GF109203X The increase of cAMP above baseline in response to 10-5M isoproterenol was studied in HASMC at control and after 30-min exposure ... reduced in human bronchi challenged withthe sensitizing allergen The effects of LTD4 in HASMC and allergen challenge in bronchial rings were prevented by the CysLT1R antagonist montelukast andthe ... experiments and helped in manuscript preparation SC participated inthe design ofthe experiments, was involved in HASMC culture, performed invitro cAMP studies and helped inthe manuscript preparation...
... Pothoideae v tộc Anthureae Trong họ Araceae, Hồng môn l chi lớn nhất, chi n y có 900 lo i, có lo i tiếng đợc trồng phổ biến nh Anthurium andreanum, Anthurium tropical v Anthurium scherzerianum Năm 1876 ... thể đến sinh trởng giai đoạn sau invitro Một trở ngại việc ứng dụng kỹ thuật nhân giống invitro v o sản xuất l giai đoạn sau invitro Do vậy, việc nghiên cứu giá thể đa ơm v chế độ dinh dỡng, ... đợc trồng v nhân giống, có 200 lo i sống hoang dại v đợc trồng nhiều nớc Anthurium andreanum đợc trồng vờn ơm để sản xuất hoa cắt Anthurium scherzerianum đợc trồng chậu l m cảnh [4] Cõy hoa h...
... conditions” In: Plant nutrition Vol.92 Springer Netherlands T¹p chÝ khoa häc vµ c«ng nghÖ n«ng nghiÖp ViÖt Nam Higaki T and Imamura JS., 1985 (a) Volcanic Black Cinder as medium for growing Anthuriums ... phôi vô tính Hồng môn Anthurium andreanum”, Viện Di truyền Nông nghiệp Hội nghị Sinh học toàn quốc, Hà Nội Dufour L 2002 “Optimization of Anthurium andreanum mineral nutrition in soilless culture ... THẢO LUẬN Thí nghiệm giai đoạn vườn ươm sau invitro 1.1 ghiên cứu ảnh hưởng giá thể đến sinh trưởng, phát triển giai đoạn sau invitro Sử dụng invitro có - lá, chiều cao trung bình - cm Các...
... obtained from the separation of polyamine solutions prepared by changing the concentration of a single polyamine and keeping the concentrations ofthe other two constant (24 mM) Inthe case of ... rough indication ofthe degree of difference in complexity between theinvitroandin vivo nuclear settings For instance, it is easy to suppose that both the presence of many other ions inthe ... ivNAPs invitro model in order to investigate some ofthe features ofthe polyamine–phosphate interactions Specifically, we examined the role played by each polyamine inthe self-assembly ofin vitro...
... ubiquitin-degrading enzyme in yeast, as well asthe identity of a catalytic triad composed of histidine, cysteine, and aspartic acid, I7L has been classified as a cysteine proteinase The thiol ... since it gave the best cleavage profile Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing ofthe core protein precursors inthein ... alanine (pI7LH241A) The extracts are prepared as described inthe Materials and Methods The extracts are mixed and incubated withthe substrates for hrs and then analyzed through SDS-PAGE and...
... failure ofthe instrumented test segment A potting jig was designed to align and hold the proximal and distal loading fixtures andthe radius during potting (Figure 1) The long axis ofthe radius was ... conceived the study, carried out the collection, potting, implanting, and testing ofthe specimens, and contributed to the experimental design WLC designed the loading-measurement system and tested the ... being tested, respectively Since the applied axial load remains coincident withthe axis through the center ofthe universal joints, this would create, as load was increased, an ever increasing...
... and conceived ofthestudy RBHI participated inthe design ofthestudyand conceived ofthestudy GK participated inthe design ofthe study, conceived thestudyand drafted the manuscript All ... (Gibco, Grand Island, NY) supplemented with 10% foetal bovine serum (FBS) (Gibco) withthe presence of antibiotics and antimycotic (Gibco), 200 mM L-glutamine (Gibco) and 50 μg/ml of ascorbic ... chondrocytes adopt many ofthe phenotypic traits of fibroblast, as they become elongated and synthesize type I collagen rather than type II collagen Thus, to induce the re-differentiation of expanded chondrocytes,...
... ubiquitin-degrading enzyme in yeast, as well asthe identity of a catalytic triad composed of histidine, cysteine, and aspartic acid, I7L has been classified as a cysteine proteinase The thiol ... since it gave the best cleavage profile Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing ofthe core protein precursors inthein ... alanine (pI7LH241A) The extracts are prepared as described inthe Materials and Methods The extracts are mixed and incubated withthe substrates for hrs and then analyzed through SDS-PAGE and...