the cell cycle instead
Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
... in the M phase of mouse FM3A cells, and phosphorylation at sites and 32 requires CDK2 during interphase in human HeLa cells Changes in the phosphorylation level during the cell cycle and the ... growing HeLa cells were incubated with nocodazole Cells detached from the bottle by shaking were collected and named mitotic (M) cells Residual cells were collected and named G2 cells These cells and ... presented at the top Mouse FT210 cells were synchronized at the G1 ⁄ S boundary by incubating the cells with aphidicolin for 16 h After removal of the drug, cells were cultured for 12 h in the presence...
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Báo cáo khoa học: Injection of poly(b-L-malate) into the plasmodium of Physarum polycephalum shortens the cell cycle and increases the growth rate pot
... until the end of the experiment We then tested whether the plasmodial size (growth rate, Fig 2A,C,E) and the duration of the cell cycle (Fig 2B,D,F) depended on the dose of injected PMLA, over the ... shortens the duration of the cell cycle is the polymer-inherent isosterism of the carboxylates with the array of phosphates in nucleic acids and, consequently, the competition with nucleic acids in the ... while almost no difference between the strains is seen for the cell cycle duration In the following discussion, these phenomena will be related to the changes in the amounts of PMLA in cytoplasmic...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
... trimethylation with chromatin during the cell cycle, we performed immunofluorescence staining of HeLa cells with anti-H3K4 trimethyl serum at different stages of the cell cycle The cell nucleus was counterstained ... cyclins E and B, as a function of the cell cycle Western blot analysis of the whole -cell extract and histones from different stages of the cell cycle demonstrated that the overall levels of MLL1 and ... methylations during cell cycle in S phase may not be the same as the marks in other phases of the cell cycle (as shown by immunofluorescence staining and western blotting); rather, old marks are...
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Báo cáo khoa học: Collective behavior in gene regulation: The cell is an oscillator, the cell cycle a developmental process doc
... urgently needed The cell cycle is a developmental process not a cycle If everything oscillates and does so with a period that is an integral submultiple of the cell cycle, then the cell cycle, as it ... between the TRAC and the cell cycle The release of H2S by a significant fraction of the cells in the fermenter ensures that no cells in the fermenter will be able to respire We have suggested that the ... products of the reaction used to represent the cell cycle in each of the cells In this case, the attractor used to represent each cell was the Rossler attractor and each cell ran the identical...
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Báo cáo Y học: The DNA-polymerase inhibiting activity of poly(b-L-malic acid) in nuclear extract during the cell cycle of Physarum polycephalum pot
... contained in the extracts The inhibitory activity during the cell cycle The inhibitory activity during the cell cycle was measured in the presence of 0.4 U of (added) purified DNA polymerase a and the ... polynucleated giant cell among other cell forms in the life cycle [4] Interestingly, the billions of nuclei in these syncytia participate with high degree of synchrony in the division cycle All of the myxomycetes ... mitosis Thus, the in vivo and in vitro activities of DNA synthesis corresponded with the minimum of the inhibitory activity in the cell cycle The activity of DNA polymerases in the nuclear extracts...
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Báo cáo y học: "Serum-dependent transcriptional networks identify distinct functional roles for H-Ras and N-Ras during initial stages of the cell cycle" doc
... during the initial stages of the cell cycle and confirm the notion of functional specificity for the H-Ras and N-Ras isoform proteins Materials and methods Cell culture Cell lines from the appropriate ... factors caused by serum withdrawal from the cultures Addition of serum to the starved (G0) cell cultures causes re-entry of the growth-arrested cells into the cell cycle, thus starting progression through ... serum-arrested (G0) cells Indeed, the available experimental evidence indicates that the contribution of Ras activity is absolutely needed for both the initial entry into the cell cycle (G0/G1 transition)...
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Báo cáo y học: " Single cell studies of the cell cycle and some models" pps
... during the growth phase in the first three quarters of the cycle but rather two linear segments with a rate change point (RCP) between them The position of the RCP showed a large cell- to -cell variation ... The selected cells come from more than a very narrow region of the cycle Some of the variation can be reduced by a correction for asynchrony [5] but there is still cell- to -cell variation in cycle ... experiments on single cells [17], they made a measurement of the dry mass of single cells by interferometry and then placed it in the cycle by following it as it moved about until it divided The difference...
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Báo cáo y học: "RNA interference pinpoints regulators of cell size and the cell cycle" doc
... regulate cell size Another aim of the screen was to identify genes whose downregulation alters cell size Becuase cells grow as they progress through the cell cycle, dsRNAs that increase the proportion ... on growth or the distribution of cells in the cell cycle These two possibilities could be distinguished by examining the effect of these dsRNAs on cell number Intriguingly, many of these hits for ... of the cell- cycle phase Thus, the group with a primarily apoptotic phenotype would contain the most effective dsRNAs, whereas the group with a cell- cycle arrest-like phenotype would contain the...
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Báo cáo y học: " The Cell Cycle Ontology: an application ontology for the..." pdf
... of the Cell Cycle Ontology [http://cellcycle onto.svn.sourceforge.net/viewvc/cellcycleonto/ONTOLOGIES] 113 CVS repository of the Cell Cycle Ontology [http://cellcycle onto.cvs.sourceforge.net/cellcycleonto/ONTOLOGIES] ... analyze the subcellular distribution of cell cycle proteins For that, we used the core cell cycle proteins subset of the CCO First, we analyzed the distribution among the three major cellular ... compartments - the cytoplasm, nucleus and cell membrane We found that the majority of cell cycle proteins are located in the nucleus (755) and the cytoplasm (356), where the majority of cell cycle events...
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Regulation of DNA (cytosine 5) methyltransferase 1 in the cell cycle and its role(s) in doxorubicin mediated micronuclei formation
... 2008) 1.4 DNMT1 in the Cell Cycle DNMT1 is constitutively expressed in mammalian cells but the expression level is cell cycle- regulated The interplay between DNMT1 and other cellular proteins ... Regulation of DNMT1 expression in the Cell Cycle 3.1.1 Inverse relationship between DNMT1 and p21WAF1 in the 63 cell cycle 3.1.1.1 3.1.1.2 DNMT1 expression in the cell cycle 63 WAF1 in DNA 67 p21WAF1 ... of the cell cycle 1.4.1 Regulation of DNMT1 expression The DNMT1 protein peaks during DNA replication in the S phase of the cell cycle, but is present at very low levels in non-cycling Go cells...
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The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression of Tumorigenesis
... complexes with E2F in the S-phase of the cell cycle (Dyson 1998) This might reflect the not yet fully understood specific functions of these proteins at these specific stages of the cell cycle 4.4 pRb Family ... pRb is the critical regulator of most cell cycle responses While each single pocket protein can mediate cell cycle arrest upon cell- cell contact, pRb is the critical mediator of cell cycle arrest ... elaborate on the role of the retinoblastoma gene family in cell cycle regulation and tumor suppression The pRb Cell Cycle Control Pathway: Components and the Cancer Connection The retinoblastoma protein,...
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The ubiquitin-proteasome pathway in cell cycle control
... Protein-ubiquitin ligases in the cell cycle core machinery One of the earliest observations relevant to the molecular basis for cell cycle regulation was periodic synthesis and destruction of major ... to the G1–S phase transition While the initial observations leading to the discovery of mitosis-specific protein degradation came from studies on the early embryonic cell cycles of Table Cell cycle ... transitions provides the advantage of irreversibility For the cell division process in particular, the uni- 168 S.I Reed directional progression through the transitions that make up the cell cycle are critical...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
... [3] We and others then observed that transcription from both cyclin B genes is controlled by combinations of tandem sites called the cell cycle- dependent element (CDE) and the cell cycle genes ... description, another report confirmed the CDE in the Cdc2 promoter as being differentially occupied by protein complexes during the cell cycle [9] 878 Mutation of nucleotides close to the CDE in the Cdc25C ... NF-Y generally contributes the largest part to promoter activity, which is then repressed through the CDE ⁄ CHR sites in the early phases of the cell cycle [11,12] Other proteins binding to CDE...
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Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf
... to the stages of the cell- division cycle as indicated below the diagram A total of 546 wild-type cells or 239 mutant (S > A)6 mutant cells have been analysed phase of the cell- division cycle Therefore, ... staining, whether the slow growth yields a cell- cycle phenotype Cells of the logarithmically growing wild-type or the (S > A)6 mutant strain were photographed and cells assigned to the respective ... to the distribution of G2 ⁄ M cells: the number of metaphase cells with a single DNA mass at the bud neck and the number of anaphase cells were increased slightly in the mutant (metaphase cells:...
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Báo cáo khoa học: Restriction point control of the mammalian cell cycle via the cyclin E/Cdk2:p27 complex doc
... early in the cell cycle (before RP) immediately suffer a long delay in the cell division cycle; (b) cells treated late in the division cycle finish the current cycle similar to untreated cells but ... of the mammalian cell division cycle A time integration for 30 h is shown for six of the intermediates of the system The G1-, S/G2- and M-phases for one cell cycle are indicated in the graph The ... regulation of the G1-S transition of the mammalian cell cycle Biotechnol Bioeng 65, 631–637 Aguda B & Tang Y (1999) The kinetic origins of the restriction point in the mammalian cell cycle Cell Prolifer...
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Báo cáo Y học: Synthesis and turn-over of the replicative Cdc6 protein during the HeLa cell cycle potx
... phases of the cell cycle, HeLa cells were arrested by a double-thymidine procedure at the G1 phase/S phase transition, and then released into the cycle after removing excess thymidine Cells were ... cell- cycle stages examined, and that about one half of the newly synthesized hCdc6p associated with chromatin during the 2-h label period Rates of hCdc6p synthesis To investigate whether the synthesis ... HeLa cells with the proteasome-inhibitor MG-132 prior to the preparation of nuclear extracts In these extracts, the phosphorylated hCdc6p in the control sample was at least as stable as the phosphatase-treated...
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Báo cáo khoa học: Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle doc
... On the other hand a recent study with human MOLT-4 cells showed no change in cyclin mRNA half-lives throughout the cell cycle [17] While most studies on cyclin mRNA stability in the cell cycle ... (Fig 2) EL4 cells advanced synchronously in the cell cycle without significant delay (Fig 2) The first fractions of cells harvested in the elutriation protocol behaved like early G1 cells They enter ... the FACS (data not shown) Northern blot analysis of mRNA from fractions of elutriated EL4 cells We needed to confirm the real-time PCR data by northern blots of RNA from elutriated cells The cell...
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Báo cáo khoa học: The inhibition of Ras farnesylation leads to an increase in p27Kip1 and G1 cell cycle arrest pdf
... Rat1/ras cells at the G1 phase of the cell cycle We next examined the effect of HR12 on the distribution of Rat1/ras cells in the cell cycle To resolve the G1, S and G2/M phases, we double-labelled the ... examine whether the effects of HR12 on the cell cycle, cell motility and cell cycle components are mediated exclusively by its effect on Ras, rather than on the farnesylation of other protein(s), ... Rat1/ras cells [[6] and this study) This raises the possibility that the HR12-induced increase in p27Kip1 levels might be the consequence of the induction of cell cell contacts, rather than the outcome...
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báo cáo hóa học:" ApoG2 induces cell cycle arrest of nasopharyngeal carcinoma cells by suppressing the c-Myc signaling pathway" pot
... cancer cells [8,9] Modifications of these cell cycle- related molecules result in cancer cell arrest at G0/G1 phase of the cell cycle However, Chang et al found that gossypol did not affect cell cycle ... examined the effect of ApoG2 on cell cycle distribution and the involved signal pathways in NPC cells The results demonstrated that ApoG2 potently arrested cells at S phase of the cell cycle We ... cancer cells and colon cancer cells [8,9] To determine whether ApoG2 could also induce cell cycle arrest in NPC cells, we performed a cell cycle analysis using flow cytometry The results showed the...
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Báo cáo sinh học: "Differences in the way a mammalian cell and yeast cells coordinate cell growth and cell-cycle progression" ppt
... where the sizes of two, unequally sized, hypothetical daughter cells are followed through several cell cycles If the cells and their progeny grow and progress through the cell cycle at the same ... and Raff Cell growth occurs in all phases of the cell cycle except M phase [1,3] Yeast cells are thought to coordinate cell- cycle progression with cell growth through the action of cell- size ... quiescent 3T3 cells by size and stimulated them to reenter the cell cycle; they found that large cells did not grow faster than small cells at the same point in the cycle, consistent with linear growth...
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