the biology and clinical importance of milieu dependent differentiation
CELL SELECTION AND RESELECTION pot
Ngày tải lên :
11/03/2014, 09:20
... SELECTION AND RESELECTION TTKTVT HCM NGUYỄN VĂN CƯỜNG VIETTEL CORPORATION CELL SELECTION AND RESELECTION TTKTVT HCM NGUYỄN VĂN CƯỜNG VIETTEL CORPORATION TTKTVT HCM CELL SELECTION AND RESELECTION THE ... Penaty_Time: Là khoảng thời gian phạt, ứng dụng cho Temporary_Offset Tham số dành để thay đổi dấu Cell_Reselecton_Offset giá trị Temporary_Offset Unit: giây, step: 20, min=20 max= 640, giá trị NGUYỄN ... PHASE2 C2 = C1 + Cell_Reselecton_Offset – Temporary_Offset * H(Penaty_Time – T) Penaty_Time 31 H(x) = , x=0 X = Penaty_Time – T Cell_Reselecton_Offset: thông số tùy chọn dương...
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Tài liệu Báo cáo " Effecting of medium composition on biomass and ginsenoside production in cell suspension culture of Panax vietnamensis Ha et Grushv " doc
Ngày tải lên :
12/02/2014, 10:20
... in the medium The maximum production of ginseng saponin and polysaccharide obtained (1.5 g/L and 2.19 g/L) at the initial nitrogen concentration of 40 mM [12] In the simultaneous production of ... at a rotation speed of 105 rpm and a culture temperature of 25o C Cells cultivated for 15 days were used in the experiment and the inoculum size g/flask (fresh weight) The other cultural conditions ... indicated that the specific production (content) of ginseng polysaccharide was not significantly affected by alteration of the N source and the saponin production was relatively higher within the initial...
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Báo cáo " Cell suspension culture Panax ginseng C. A. Meyer: Role of plant growth regulators and medium composition on biomass and ginsenoside production " docx
Ngày tải lên :
14/03/2014, 10:20
... in the medium The maximum production of ginseng saponin and polysaccharide obtained (1.5 g/L and 2.19 g/L) at the initial nitrogen concentration of 40 mM.[14] In the simultaneous production of ... SAS Institute Inc., Cary, USA) Results and discussion To understand the culture characteristics of the suspended cells of ginseng in shake flask, the effect of plant growth regulators (2,4-D or ... initial nitrogen concentration of 40 mM and the cell growth was inhibited at a high initial nitrogen concentration of 80 mM Similarly, the accumulation of total saponin and polysaccharide were also...
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Báo cáo khoa học: Super life – how and why ‘cell selection’ leads to the fastest-growing eukaryote doc
Ngày tải lên :
16/03/2014, 04:20
... only by the buffering capacity of the inowing medium (BCR, dened as the amount of acid or base required to change the pH of L of the medium in the reservoir to the pH of the medium in the culture ... number of protons are produced per unit biomass synthesized The rate of growth is independent of BCR and DpH, and depends only on the conditions under which the micro-organism is cultured and the ... components and environmental conditions at constant pH, temperature and biomass concentrations [1,56] The rate of growth was independent of the buffer capacity of the inowing fresh medium and the pH...
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Báo cáo " Effects of macro elements on biomass and ginsenoside production in cell suspension culture of Ngoc Linh ginseng " docx
Ngày tải lên :
28/03/2014, 10:20
... Concentration of macro-element Fig Growth yield of ginseng cell was affected by concentration of macro elements Values are the quotient of the root dry weight after weeks of culture and the cell dry ... Results and discussion Effects of macro elements on biomass and ginsenoside production Table and Figure show how growth and yield of ginseng cell were affected by the concentrations of macro ... at a rotation speed of 105 rpm and a culture temperature of 25oC Cells cultivated for 15 days were used in the experiment and the inoculum size g/flask (fresh weight) The other cultural conditions...
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Báo cáo khoa học: ERK and cell death: ERK location and T cell selection pdf
Ngày tải lên :
29/03/2014, 08:20
... of the selecting ligand In addition, the kinetics of the other MAPK important for negative selection are the same independent of the ligand Given their opposing function and downstream targets, ... activity and subcellular location are the same regardless of the ligand strength, ERK location and T cell selection whereas ERK activity and location change depending of the nature of the selecting ... considering the redundant role of the related family member Nor1 in mediating clonal deletion and the fact that the dominant negative form of Nur77 is able to inhibit the function of the other family...
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Báo cáo y học: "VLA-4-dependent and -independent pathways in cell contactinduced proinflammatory cytokine production by synovial nurselike cells from rheumatoid arthritis patients" potx
Ngày tải lên :
09/08/2014, 06:22
... were then plated onto the monolayer of RA-SNC77 cells and further incubated for 48 hours The culture supernatants were harvested, and the concentrations of IL-6 and IL-8 were determined by the ... plated onto a monolayer of RA-SNC77 cells and incubated for hours The lymphoid cells bound to the surface of the RA-SNC77 cells were removed, and the interaction between these cells was examined ... plate After 30 of coculture, the nonadherent cells were removed and the fluorescence intensity of the adherent cells was measured Results are expressed as the means ± standard deviation of three different...
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Báo cáo y học: "B cells in Sjögren’s syndrome: indications for disturbed selection and differentiation in ectopic lymphoid tissue" doc
Ngày tải lên :
09/08/2014, 10:20
... & Therapy Vol No Hansen et al that interactions of activated SGECs and endothelial cells with the infiltrating lymphoid and dendritic cells contribute to the perpetuation and progression of the ... persistent swelling of the major salivary glands, the lymphocytic lesions of the involved glands often contain massive lymphoid infiltrates with secondary lymph follicles and/ or intraglandular lymph ... further delineation, the available data may provide paths to understanding the underlying pathogenetic mechanisms of this entity In particular, the awareness of the involvement of B cells in the...
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Báo cáo y học: "DNA-like class R inhibitory oligonucleotides (INH-ODNs) preferentially block autoantigen-induced B-cell and dendritic cell activation in vitro and autoantibody production in lupus-prone MRL-Faslpr/lpr mice in vivo" potx
Ngày tải lên :
09/08/2014, 14:21
... proven whether the same type of the crosstalk between BCR and TLR also occurs between antigen and co-delivered TLR7 ligand These findings have direct implications for the pathogenesis of systemic ... triplets INH-4 and INH13 are palindromic or linear analogues of INH-1 and INH-18, with the difference that the canonical CCT and GGG blocks are placed at the 3' end of the molecule We have further created ... Depending on the nature of the initiating autoantigen, the proliferation requires either the TLR7 or TLR9 pathway, including the presence of the key adaptor protein MyD88 (myeloid differentiation primary...
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Báo cáo y học: "Type I interferon receptor controls B-cell expression of nucleic acid-sensing Toll-like receptors and autoantibody production in a murine model of lupus" doc
Ngày tải lên :
09/08/2014, 14:22
... will therefore be critical to assess the function of TAM receptors and the differential roles of IFNAR1 and IFNAR2 in the development of autoimmunity Unlike other murine models of SLE, such as the ... contributed to the experimental design and supplied the IFNAR2-/- mice PJU assisted with conception of the study idea and participated in its design, data analysis, and the writing of the manuscript ... demonstrated that the IFN-I signaling molecules, IRF9 and STAT1, were required for the production of IgG autoantibodies in the pristane model and for the high expression levels of TLR7 and TLR9 following...
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Báo cáo y học: " Transcriptome analysis of functional differentiation between haploid and diploid cells of Emiliania huxleyi, a globally significant photosynthetic calcifying cell" potx
Ngày tải lên :
09/08/2014, 20:20
... protons out of the cytoplasm Here we identified homologs of five out of the six subunits of the V0 domain and six out of the eight subunits of the V1 domain known in yeast Many of these subunits ... curation of the whole genome sequence of E huxleyi and for improved understanding of coccolithophorids and haptophytes The greatest gap in current understanding of the ecology and natural history of ... sets based on the time of sequencing (that is, the first and the second rounds of sequencing) and by counting the number of clusters represented by either or both of the two sets The ML analysis...
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báo cáo khoa học: " Effect of clone selection, nitrogen supply, leaf damage and mycorrhizal fungi on stilbene and emodin production in knotweed" doc
Ngày tải lên :
11/08/2014, 11:20
... potential and a number of clones of the hybrid can be considered as the most successful representatives of the genus in terms of growth rate, regeneration and the establishment of new shoots The species ... study the “inland” sources of resveratrol and other stilbenes in F xbohemica, along with the other parental species, F sachalinensis The resveratrol and piceid contents in these plants, in terms of ... the various efficiencies of the variety of extraction agents used or due to the measurement of the extract rather than the whole plant We measured the stilbene yields of these plants under specific...
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Báo cáo y học: "Dendritic cell subsets dynamics and cytokine production in SIVmac239-infected Chinese rhesus macaques" pptx
Ngày tải lên :
13/08/2014, 01:20
... 10 μl of Premix Ex Taq, μl of the standard or samples, 0.4 μl ROX reference Dye II, 200 nM of each primer, and 100 nM of the TaqMan probe The probe and primers were designed to bind within the ... level because of the death of 00317 and 98081 (Figure 3A) The mean count of mDC reached the highest site on days 202 and 265, but the value was not significantly increased (P = 0.144 and P = 0.273, ... be explained by the migration of DC into the lymph node Then the value gradually returned to the subnormal baseline level before day 76 and increased Finally, the number declined and remained at...
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Báo cáo y học: "The global role of ppGpp synthesis in morphological differentiation and antibiotic production in Streptomyces coelicolor A3(2)" pdf
Ngày tải lên :
14/08/2014, 08:20
... influence of ppGpp synthesis on the biology of S coelicolor, with particular emphasis on the processes of morphological differentiation and secondary metabolite production Classically, the effects of ... production and morphological differentiation during growth The results extend the known involvement of ppGpp synthesis in the regulation of antibiotic and secondary metabolite production, and paint ... GeneSpring they exhibited subtly different expression levels for a minor subset of genes when compared to the other M600 samples These, and the M570 data from the corresponding times, were therefore...
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Study of human nasal epithelial stem or progenitor cell growth and differentiation in an in vitro system
Ngày tải lên :
09/09/2015, 11:29
... investigation of biophysiology and pathophysiology of cell growth and differentiation of hNESPCs isolated from xii healthy nasal mucosa and NP biopsies, and the implication of this cell model in the study ... NPs Epithelial repair and damage As the results of genetic predisposition and inflammatory cells disorder, the capacity of epithelial repair is decreased, leading to the imbalance of epithelial ... culture These results indicated that we had successfully isolated and expanded pure hNESPCs in vitro Secondly, we investigated whether the growth and differentiation properties of the nasal epithelial...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
Ngày tải lên :
09/09/2015, 17:54
... dorsal ectoderm and dorsalizes lateral and ventral mesoderm Noggin binds and inactivates the BMPs, and the inhibition of the BMP signaling pathway in the ectoderm is the hallmark of neural fate ... 2010) Others such as Evf2 modulate the activity of their protein partners (Feng et al., 2006) 25 Chapter II – Aims and Objectives 2.1 Main goals of the thesis The central aim of this thesis ... the six- to eight-nucleotide “seed sequence” of the miRNA The miRNA then inhibits translation or cause degradation of the mRNA by the RISC complex when there is perfect complementarity with the...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
Ngày tải lên :
09/09/2015, 17:54
... hESCs and NPCs (Figure 5.1) In addition, the expression profiles of these lncRNAs mirror that of known drivers of neuronal differentiation (Figures 5.2E-F), and were therefore indicative of important ... stem cells, and knockdown of these lncRNAs resulted in the loss of pluripotency, coupled with the induction of early differentiation markers Their low transcript abundance suggested that they might ... sites of the lncRNAs (Figure 6.4) The proximity of these binding sites suggests that the lncRNAs may be direct, downstream targets of pluripotency factors OCT4 and NANOG To test this, the expression...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
Ngày tải lên :
09/09/2015, 17:54
... 7.5 and 8.7) Knockdown of RMST appeared to shut down the neurogenic program of neural stem cells, with the cells adopting a glia lineage instead (Figure 7.6) On the other hand, overexpression of ... protein bands corresponding to 37 kDa and 35 kDa were observed in the RMST pulldown, and not in the controls (beads only and antisenseRMST, AS-RMST) These bands were excised for mass spectrometry and ... Cellular RNA was separated into the nuclear and cytoplasmic fractions by RNA fractionation, and abundance of the RNA transcripts in either fraction was assayed by qPCR The nuclear/cytoplasmic ratio...
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