the active coefficient of lat eral
Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf
... activity after h We then applied the technique of N-terminal pegylation to visualize the relative solvent exposure of the N-terminus of FVIIa To the best of our knowledge, this is the rst time that ... circumstances, the presence of sTF protected the N-terminus of the protease domain from modication In a control experiment, no pegylation of zymogen FVII (R152A-FVII), i.e of the N-terminus of the c-carboxyglutamic ... in the vicinity of the S1 pocket [11,34], in the rst part of activation loop 3, and in the area of the TF-interactive helix and the 170 loop [8,35], areas that are stabilized by TF [9,25,26] The...
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Tài liệu Báo cáo khoa học: Site-directed mutagenesis of a loop at the active site of E1 (a2b2) of the pyruvate dehydrogenase complex A possible common sequence motif docx
... the entrance to the active site (Fig 1), as the main cleavage sites Cleavage of the E1a-subunit activated the enzyme, as determined by a decarboxylation assay of the free E1 in the presence of ... detected by the PDH assay The interaction of E1 (a2b2) with the PSBD was unaffected by any of the mutations in E1a (Fig 4) Therefore, any effects of the mutations on the catalytic activities of the PDH ... affecting the efficacy of the recognition of the tethered lipoyl domain by E1, an essential prelude to reductive acetylation of the pendant lipoyl group [5,9,39] The damaging effects of mutations in the...
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Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc
... indicated by the MD simulations of the modelled ternary hGK–Glc– ATP complex In the final structure of the simulations, the interactions of the adenosine moiety were similar to those observed in the binary ... is compatible with a direct interaction of these residues with the nucleotide at the active site The catalytic mechanism of GK has been the subject of several detailed analyses, and is still a ... displaced in the direction of Glc, and there was no measurable closure of the active site cleft during the 2-ns MD simulations (Fig 5C), as observed in the crystal structures of the binary GK–Glc complex...
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Báo cáo khoa học: Interactions of imidazoline ligands with the active site of purified monoamine oxidase A potx
... and the I2BS stereospecificities would be useful Interestingly, the lowest-energy orientation for each of the three ligands in the large active site of MAO-A put them in the middle of the active ... and the values for 2-BFI and idazoxan are given in Table Evidence for the proximity of these ligands to the flavin in MAO-A comes from the influence of the ligand on the redox properties of the ... observed The transfer of electrons from the amine to the flavin requires a catalytic interaction, so the nitro˚ genous group of agmatine must be within A of N5 of the isoalloxazine ring of the flavin...
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Báo cáo khoa học: Site-directed mutagenesis of selected residues at the active site of aryl-alcohol oxidase, an H2O2-producing ligninolytic enzyme pot
... found after each docking calculation, and in all cases more than 50% of them clustered together in front of the rectus (re)-face of the isoalloxazine ring of the FAD cofactor This substrate location ... they are situated at similar distances from the hydroxyl of the docked substrate, they could cooperate in facilitating the hydride transfer from substrate to FAD The decrease of activity of the ... oxidase, the flavin domain of cellobiose dehydrogenase and cholesterol oxidase The positions of the conserved histidine and histidine ⁄ asparagine residues near the FAD isoalloxazine ring of four of the...
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
... positioned in the ÔupÕ position rows and shows that in the presence of the substrate, rotation of Asp142 towards Glu144 lowers the pKa of the latter by 0.8 pH units The calculated effects of the D140N ... for the position of residue 142 [34] The pKa calculations indicate that the only candidate for a residue determining the basic arm of the pH-activity profile of the D142N mutant is Glu144 The ... where the enzyme is most active Summarizing, the calculations show that the carboxylic groups in the Asp140-Asp142-Glu144 triad contain two protons at the pH where the enzyme is most active The...
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Báo cáo khoa học: Homoadenosylcobalamins as probes for exploring the active sites of coenzyme B12-dependent diol dehydratase and ethanolamine ammonia-lyase docx
... Difference in the specificity for the adenosyl group among enzymes may reflect the difference of the adenosyl group-binding sites of the enzymes From simulation of the EPR spectra of reacting holoenzymes, ... with those of free counterparts The extents of the Co-C bond cleavage of these analogues were negligible although that of AdoCbl was estimated to be 85% from Fig 3E Upon denaturation of the enzyme-cobalamin ... enzymatic activation (labilization) of the coenzyme Co-C bond, the structure–function relationship of AdoCbl and the role of each structural component of the coenzyme were extensively studied...
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Báo cáo khoa học: Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center? Supporting amino acid residues at the active site of penicillin G acylase pptx
... group in the proximity of the oxyanion can be found in the rest of the Ntn hydrolases [3] From another point of view, the hydrogen bond between the oxyanion and the aNH3+ of SerB1 resembles the one ... between the carboxylate group of the Asp residue and the protonated imidazole ring of the His residue in the serine protease catalytic triad In the second TS (TS2) of the expulsion of the p-substituted ... because of the substitution of the oxyanion hole with two water molecules The effective stabilization of the oxyanion is the main driving force of the first step of acylation, and dominates all other...
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Báo cáo khoa học: Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains pdf
... A) to the amide nitrogen of Leu709 from the second molecule The orientation of the side chain of His667 is also maintained due to the proximity of the negative charge of the side chain of Glu706 ... availability of a large number of genomic sequences has significantly increased the number of identifiable analogs of E coli Lon and prompted a reanalysis of the active sites of this family of proteases The ... analysis of the amino acid sequences of the majority of the currently known Lon proteases The results of site-directed mutagenesis of E coli Lon protease and insights from the crystal structure of...
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Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx
... required for the cofactor inhibition of phosphorylation (data not shown) The molecular basis for the negative modulatory effects of the pterin cofactor and dopamine binding to wt-hPAH and their reversal ... contains both the regulatory and the catalytic domains (residues 1429), were performed to demonstrate the interactions of the dihydroxypropyl side-chain of the pterin cofactors with the N-terminal ... and the [S]0.5-value for L-Phe increased to 178 11 lM The presence of 500 lM of the oxidized cofactor H2biopterin in the running buffer also lowers the ể FEBS 2003 Regulatory properties of phenylalanine...
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Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1 ppt
... construction of a CYP73A1 model, the identification of residues likely to form contacts with the substrate, and the confirmation by site-directed mutagenesis of the involvement of some of these residues ... model of the active site of CYP73A1 Construction of this first model was based on four bacterial crystallized structures Only part of the active site is shown Based on the 1H-NMR data [10], the ... hindrance to the binding of the substrate above the haem iron As expected, the I371A mutation substantially decreases CA affinity and the ability to desolvate the active site Around 10% of the catalytic...
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Báo cáo Y học: Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase A site-directed mutagenesis study docx
... release from the EredIP complex All of these results point to a role of the Y238 side chain in substrate/product exchange to the active site of RgDAAO A superimposition of the active sites of yeast ... change [10] The higher stabilization of the semiquinone form observed for the Y238S mutant in the free form may be the result of a better interaction of R285 with the N(1)-C(2)ẳO locus of the reduced ... equilibration method of Minnaert [17], in order to assess changes in the thermodynamic properties of the avin centre caused by the mutation and to explain the different thermodynamic stability of the semiquinone...
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Báo cáo Y học: Characterization of the active site of histidine ammonia-lyase from Pseudomonas putida pptx
... facilitates the electrophilic attack at the methylene moiety of MIO by decreasing of the electron density in the p system of the C¼C double bond (Fig 5) Calculation of the UV spectra of MIO and the energy ... of the carboxylate group of the urocanate caused by its conjugation, i.e coplanarity to the p system of the forming C¼C double bond, the carboxylate moiety is displaced from the vicinity of the ... in these cases (Fig 8C) These results indicate the presence of a MIO at their active sites, but in a less reactive form Concomittant with the formation of a new chromophore the activity of the...
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Báo cáo khoa học: Mapping of the active site of glutamate carboxypeptidase II by site-directed mutagenesis potx
... distortion of the coordination sphere of the active- site zincs Our results indicate that the binding of the glutarate part of the inhibitor in the S1¢ pocket contributes to the inhibition effect of the ... above, the mutation Y552I leads to the decrease of both the Km and kcat values The mutation of Gly518 to Pro led to a slight increase of the Km value and one order of magnitude decrease of the kcat ... role of the S1¢ residues is also supported by the fact that all of them are conserved in the GCPII homolog GCPIII and in the mammalian GCPII orthologs (Table 4) and that the ability of these...
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Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334fiGly mutant enzymes pot
... 7.0) and the catalytic rates of the H334G mutant in the absence (0.0015 s)1; pH 6.5) and the presence (0.0081 s)1; pH 7.0) of imidazole The lines indicate the trend of the data signal of PLP phosphate ... accounting for the relative elevation of pKa of PLP phosphate in unliganded CcStP and the apparent lack of perturbation of pKa in the CcStP complex with arsenate The pH dependence of the 31P chemical ... to the H334G mutant This result is in contrast to the 17-fold enhancement of GL binding to the wild-type upon the addition of the same concentration of phosphate pH profiles The pH dependences of...
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Báo cáo Y học: Contribution of Lys276 to the conformational flexibility of the active site of glutamate decarboxylase from Escherichia coli pptx
... alteration in the polarity of the active site In this hypothesis, the environment of the cofactor is more hydrated in the lowpH conformation Thus, the increased polarity favors the 420 nm-ketoenamine ... tautomer of the wild-type cofactor and the 388 nm-absorbing unhydrated aldehyde of PLP in the mutant enzymes (Fig 6) Fig Chemical structures of the chromophore proposed to be present at the active ... positioning the Ca-COO– bond orthogonal to the plane of the delocalized cofactor imine system Moreover, the release of cofactor as PLP when the enzyme was treated with NaOH after completion of this...
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Báo cáo y học: "The active metabolite of leflunomide, A77 1726, increases the production of IL-1 receptor antagonist in human synovial fibroblasts and articular chondrocytes" ppsx
... PGE2 synthesis was observed in the presence In the present study we investigated the effect of the active metabolite of leflunomide – A77 1726 – on the production of IL-1Ra by human joint cells ... mediating the stimulatory effect of A77 1726 on IL-1Ra production, first focusing on the known effect of A77 1726 on pyrimidine synthesis Addition of exogenous uridine did not significantly modulate the ... enhancing the production of secreted IL-1Ra, A77 1726 also increased the amount of IL-1Ra present in cell lysates of synovial fibroblasts stimulated with IL-1β (Fig 2a) IL-1β alone stimulated the production...
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Báo cáo y học: "The active metabolite of leflunomide, A77 1726, interferes with dendritic cell function" doc
... impaired the T-cell stimulatory capacity of DCs As a molecular basis for the ability of LEF-M to interfere with several aspects of DC function, the activation-driven nuclear transmigration of the ... plus IL-4 (10 ng/ml) in the presence or absence of the indicated concentrations of LEF-M Dendritic cells (DCs) differentiated in the presence of the active metabolite of leflunomide (LEF-M) are ... [LPS]) in the presence or absence of the indicated concentrations of the active metabolite of leflunomide (LEF-M) Cell-free supernatants were collected 48 hours after addition of LPS and then analyzed...
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phosphatidylserine exposure in red blood cells: a suggestion for the active role of red blood cells in blood clot formation
... proteins The functions of the membrane proteins are mostly regulated by the state of phosphorylation, methylation, glycosylation, or lipid modification (myristylation, palmitylation, or farnesylation) ... also a problem of concern because it relates to the process of ageing and removing of old RBCs out of the blood circulation Regarding young and old RBCs, it has been speculated that the intracellular ... main work of this thesis has been focused on the relation of intracellular Ca2+ and PS exposure in RBCs Factors related to the PS exposure and the relations between the ageing of RBCs and eryptosis...
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Coefficient of consolidation from the linear
... Color profile: Disabled Composite Default screen Feng and Lee point from the U–T1/2 plot in Fig of the discussed paper is not as difficult as that implied by the discussers 1003 Taylor, ... paper is not as difficult as that implied by the discussers 1003 Taylor, D.W 1948 Fundamentals of soil mechanics John Wiley & Sons, New York 700 pp Feng and Lee References Casagrande, A., and...
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