... for maintaining a balanced supply of the DNA precursor This underlines the importance of elucidating the molecular and structural background of the enzymatic and catalytic properties of human thymidine ... at )80 °C before use for kinetic and molecular mass analyses The activity at saturating conditions was similar before and after dilution, incubation and storage Native molecular size The apparent ... analogous totheaand b phosphate groups inthe nucleotide ADP On the other hand, the large difference in phosphate donor capacity, only 4% with ADP and no activity with NaPPP and NaPP, indicates that...
... forward 5¢-GGGGACAAGTTTGTACAAAAAAGCAG GCTCCATGCCTCAGTTACTGCAAAACATTAATGGG ATCATCGAGGCC-3¢; reverse 5¢-GGGGACCACTTTGT ACAAGAAAGCTGGGTCGGCCAGCGGCTTAAGGTT TTATTGATGCATTAGGGTAGATGGGGC-3¢ Human SEP53 ... standard to quantify the bile acids from patients Standard ratios represent the peak area of each mgÆmL)1 standard compared with the peak area of the internal standard (B) Summary of the range ... of the calcium-binding domain of SEP53 attenuated is activity as a survival factor ina clonogenic assay, we also evaluated whether theresponseto DCA required the calcium-binding domain Stable...
... ATGGGATATGTTCTCAGT ACAGATTTACGACTCCTCCTG CATGTTGCTGTAGCAGTTTGAT ATATAAGCTTATCCTCTGATAGC GACCCATCATCGAGGACTA ACCACAAGGGTTTCAAGCAG CCACACCAGGAAGGTCTTGT GGTGGAGGAAACCTTGGACT ACGTCAACATGTCCGACAAA ... AK LF AK SF AK LR AK SR eIF 4A LF eIF 4A SF eIF 4A LR eIF 4A SR CAATCCATCAAAACCGTGTG TGCTACAGCAACTGGTGATCAGAAGGG CCCTTCCTGATCACCATGTTGCTGT GGCCATCATACAGGTGACTAGGAGGGT GGGTGATTTGACACACGGTTTTGATGGA ... a more than 30-fold increase in lactate, whereas the levels in controls remained constant The arginine kinase gene (AK) andthe eukaryotic translation initiation factor 4A gene (eIF 4A, DQ667140)...
... roles during the execution phase of apoptosis [24] We observed a parallel activation of these proteases, the activation of caspase being transient andthe activation of caspase being stronger and ... (Nottingham, UK) Immunoblotting with the following antibodies against the indicated marker proteins were carried out as control: anti-calpain for the cytoplasmic fraction and anti-(insulin receptor) ... N-acetylTyr-Val-Ala-Asp-7-amino-4-metylcoumarin for caspase 1, N-acetyl-Asp-Glu-Val-Asp-7-amino-4-metylcoumarin for caspase 3, N-acetyl-Val-Glu-Ile-Asp-7-amino-4-trifluorometylcoumarin for caspase...
... hypoxia could stimulate TRAIL signaling, leading tothe activation of two distinct pathways One pathway might lead to increased nuclear localization of BRCA1 andthe other to apoptosis Hypoxia could ... BRCA1 shRNA Thus, these data support the hypothesis that BRCA1 enhances TRAIL’s ability to mediate apoptosis in breast cancer cell lines Discussion There is increasing interest inthe association ... receptors, DR4 and DR5, and recruits Fas-associated death domain protein tothe receptors Caspase-8 is activated and subsequently activates downstream caspases Hypoxia inhibits TRAIL-mediated apoptosis...
... 5¢-ATCTGGAGAGCACATCATTGCTGGTGCA-3¢ 5¢-ACATCGAGGAAGAGTTTTCTATCCTGGA-3¢ 5¢-ACATCGCTGAGAATGTCAACGGGGATAT-3¢ 5¢-CTTTCTGGCCTCTCCAACATCCATGCCA-3¢ 5¢-ATGCCAAGGTAGTTTATGATGATCGAGA-3¢ 5¢-TCTCCTACGATCATCTCACCGTCACCGA-3¢ ... 5¢-GCACAGTTCCCCTACAGTCCCGCTTTAG-3¢ 5¢-CACGTCTCAGCAGGGAGAATAATCCCGA-3¢ 5¢-GAGCTCAGCGAGGACGGAAACCTCGCGT-3¢ ADI (up) 5¢-CCAATCAGGTAGGCCTTCATGGAGAGGA-3¢ 5¢-CCCAGAGATCCTCCAAGAGACAGCCAGT-3¢ ADI (up) ADI (down) ADI (down) 5¢-ATCTGGAGAGCACATCATTGCTGGTGCA-3¢ ... ADI and G (up) ADI (up) G (up) G (up) 5¢-GTGCATCAAAGAATTTTGGATAC-3¢ 5¢-AGAGAAGTGGCAGCTTTCGCTCAGTTTGG-3¢ 5¢-CTCGGCAAAGAAACCGCTGGTTCCTCCCA-3¢ 5¢-GAGACGTCCAGGAAATCTTCCGCAACACC-3¢ 5¢-CAGAGTGTGCACTAGCATGCGGTCCCGT-3¢...
... Castanopsis fargesii and by Wiebel et al [53] for Garcinia mangostana In addition, an increasing number of branches of young Quercus petraea and an increasing length growth of these branches with increasing ... for placing the site for the experiment at my disposal and for their assistance in constructing the wooden frames of the nets and measuring the seedlings I also thank K Thoroe for measuring the ... Seeding and Planting at Teisendorf (Bavaria, Germany) in open field All seeds of a species originated from the same stand, which was selected according tothe law on forest reproductive material...
... a substance such as ABS are harmful to oaks The morphological and physiological alterations tothe leaf waxes have biological consequences, increasing the cuticular transpiration rate and leaving ... were sprayed again in 1991: initial data revealed structural degradation of the leaf waxes similar to that of the year before, but the relative tolerance among the species was unchanged Inthe culture ... analysis, is common along the Tuscan coast andin hilly areas further inland and for several years has exhibited abnormally intense flowering (Gellini and Paoletti, 1990) Pollen for our study was...
... NRP -A and NRP-B, an ubiquitin-associated (UBA) protein homolog and NAC (NAM, ATAF1, ATAF2 and CUC2) domaincontaining proteins NAC proteins are plant specific transcriptional factors that are involved ... phenotypes, and acts antagonistically to suppress ABA-mediated responses As ABA isa central regulator of plant adaptation to drought [30,31] and plays a crucial role inthe regulation of transpirational ... bacterial pathogen attack and induce a defense response [25], and then we inoculated soybean leaves with the incompatible bacterium, Pseudomonas syringae patovar tomato (Additional file 3), as...
... (5’-AAGCTTATGAGA GGTCGTATAAGTA-3’), containing a HindIII site, and Vv60R3 (5’-TCTAGAGGCCTTCCTATGGCTT-3’), containing an XbaI site, andthe PCR fragment was cloned inthe pBI101.3 vector The VvMYB30 ... (5’-GGATCCATGGGAAGGCCTCCTTGCT-3’) and Vv30R2 (5’-AAGTCTGACAGTGATGAGAGGAGC3’); Vv60F2 (5’-CTCCTTGCTGTGATAAAGTTGGTAT3’) and Vv60R2 (5’-ATTCAGGTTTTCGTACTCAAGAATG-3’) The control AtACTIN2 gene (At3g18780) ... t-test) (H) and (I) Histochemical analysis of GUS expression in pVvMYB60:GUS leaves inresponseto ABA (H) GUS staining of stomata ina control leaf (I), GUS staining of stomata following hours...
... along the Appalachian Mountains to Alabama and westward tothe Mississippi river [2] In some areas up to 45% of the forest canopy was comprised of American chestnut [3] This large, fast-growing ... transcriptome characterization and gene discovery Transcriptome comparison between canker tissues from Castanea mollissima and Castanea dentata GO annotation analyses showed that, overall, canker tissues ... protein This protein kinase activates both local resistance and basal resistance [38,46,47] It also appears from our data that metabolic flux may be involved inthe chestnut resistance to the...
... performed; Andrea Pinczak, Leticia Silva, and Mariana Laca for help with animal management; Ignacio Videla (Syntex SA, Buenos Aires, Argentina) for providing us with the sponges and Novormón; and Ricardo ... Iglesias for laparoscopic observations Thanks are also due to M. -A Carlsson and Å Karlsson for RIA analysis, andto Dr A. F Parlow and Dr J Roser for supplying hormone and antibody preparations ... experiment was carried out on a commercial farm near Trinidad, Uruguay (33° SL), during the mid-breeding season (April-May) Alto- Endocrine and ovarian changes inresponsetothe ram effect gether 71...
... organism [6,7] Inthe initial phase of infection, Listeria binds to splenic macrophages andis internalized; Listeria produces products that activate nuclear factor-kappa B and upregulate innate ... cytokine is not absolutely linked tothe initial clearance of L monocytogenes, these findings are consistent with the notion that the RAGE-induced proinflammatory state may be deleterious inthe ... Pamer [5], inthe first few days of Listeria infection, the innate responseis critical for early bacterial clearance and host survival The adaptive response instead is required for controlling...
... 16) at t1 and M = 9.16 years (range - 16) at t2 The Tanzanian and German board of the organization managing the orphanage gave their consent and ethical approval Materials The interview sets ... before in other Sub-Saharan African settings The translators were extensively trained andthe translation was discussed in detail Nevertheless, cultural bias might have influenced the findings, as ... developmental stages, attachment, and bonding was given tothe caretakers to foster their understanding and empathy towards the children Grief: As many of the children have lost their parents also some...
... To analyze the financial situation of the business need to use the data from the financial statements of the business for some of years and compare these indicators together The indicators can ... OIL’s business strategy Comparison of financial analysis indicators for 2011 and 2010; 2010 and 2009; 2011 and 2009; Table 2.3.3.5b Analysis of financial indicators FINANCIAL INDICATORS SS 11 ... managers and experts of the PV OIL and experts are active staff inthe field of oil and gas business inthe company of Vietnam In addition, the research information and data to be gathered and...
... proliferator-activated receptor-alpha (PPARa) and peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1), pivotal nuclear regulators of numerous fatty acid metabolic genes [10] In ... and Discussion The main finding of this study isthe identification of a metabolic gene switch from fatty acids to glucose inthe murine female heart at baseline linked to enhanced mitochondrial ... myocardial glucose metabolism may be increased in parallel As optimization of glucose metabolism is increasingly highlighted as a therapeutic intervention for ischemia-induced and ischemia–reperfusion-induced...
... ⁄ PAGE After drying of the gels, labeled proteins were visualized by autoradiography, and quantified by a PhosphorImager with imagequant 5.0 software MS The N-terminal amino acids and internal ... left) After incubation at 30 °C for 15 min, the mixtures were separated on 5% polyacrylamide gels, and then subjected to autoradiography The retarded DNA fragments are indicated (a, b, c and d) The ... (GGGCGTCGG) near to its 3¢-end (data not shown) Thisisin accordance with the fact that the helix–turn–helix DNA-binding domains of SenR and ChrA share 61% amino acid identity (data not shown) The designed...
... Acetoacetic Ester Acetoacetic Ester O H3C O C C H C OCH2CH3 H Acetoacetic ester is another name for ethyl acetoacetate The "acetoacetic ester synthesis" uses acetoacetic ester as a reactant ... OCH2CH3 The anion of ethyl acetoacetate can be alkylated using an alkyl halide (SN2: primary and secondary alkyl halides work best; tertiary alkyl halides undergo elimination) R 21-6 Conversion to ... CO2 Saponification and acidification convert the alkylated derivative tothe corresponding β-keto acid The β-keto acid then undergoes decarboxylation to form a ketone 21-8 Example Example O O CH3CCH2COCH2CH3...
... USA) This vector encodes N-terminal S- and HIS- tags and C-terminal HIS- and HSV-tags The fragments were over expressed in E coli and purified using Ni-NTA agarose (Qiagen, Valencia, CA, USA) ... molecular mass of 168 kDa, this indicates that CSB isa dimeric protein DNA was not present in these fractions since the ATPase activity was only detectable after the addition of pUC19 DNA This indicates ... advance the understanding of the mechanism by which the DNAdependent ATPases, in general, and CSB, in particular, functions Furthermore, oligomerization status is important to evaluate the stoichiometry...