tgf β signaling in embryonic stem cell derived endothelial cells pdf
Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... difficulty in isolating pure early stage BMP2-expressing cells in sufficient quantities during early embryonic development in vivo Extensive investigations applying the in vitro embryonic stem (ES) cellbased ... BMP2-expressing cells during early differentiation of ES cells will pave the way for a better understanding of NCSCs and their differentiation into lineages The BMP2+ cells derived from the ES cells ... transcripts belonging to the TGF- β signaling pathway that are specifically upregulated in the BMP2+ cells as well as a schematic of the KEGG TGF- β signaling pathway indicating the upregulated...
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Báo cáo y học: "Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes" doc
... Hescheler J, Sachinidis A: Embryonic stem cells: a promising tool for cell replacement therapy J Cell Mol Med 2004, 8:465-473 Winkler J, Hescheler J, Sachinidis A: Embryonic stem cells for basic ... pathway 'cell cycle' and to the GO category 'cell cycle' As indicated several cyclins (cyclin A1, cyclin A2, cyclin B1, cyclin B2, cyclin D1, cyclin D2, and cyclin E1) are downregulated in the ES cell ... cardiomyocytes derived from embryonic stem (ES) cells Mouse ES cells can proliferate indefinitely without senescence in vitro in their undifferentiated state in the presence of leukemia inhibitory...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
... deciphering the mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell ... feeder-free conditions according to the technical manual “Maintenance of Human Embryonic Stem Cells in mTeSRTM1” (STEMCELL Technologies Inc.) In brief, H1 hESCs were maintained on BD matrigel hESC-qualified ... being developed to address these problems (Cheng et al., 2012) An alternative to reprograming of terminally committed cells into iPS cells and then deriving NSCs is the direct reprograming of cells...
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The role of microRNAs in embryonic stem cell development and differentiation
... receptor Multiple cloning site Methyl-CpG-binding proteins Mouse embryonic stem cell Midbrain-hindbrain isthmus/boundary MicroRNA MicroRNA MicroRNA-containing RNA-induced silencing complexes MicroRNA ... carcinoma (EC) cells, which resemble early embryonic cells (Martin and Evans, 1975) EC cells could be expanded continuously in culture while retaining the capacity to differentiate into derivatives ... saline double-stranded RNA-binding domain Embryoid body Embryonic carcinoma Early embryonic microRNA cluster Embryonic stem cell Fetal bovine serum Fragile X mental retardation-related protein...
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Báo cáo y học: "Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection" pptx
... HIV-1 infection is evident in virus exposed HD cells Since cells of the hematopoietic system are among the main targets of the HIV-1 virus, we investigated the effect of viral exposure on HD cells ... exposure on HD cells, we compared the expression of selected genes in HD-HIV cells versus un-infected HD cells The gene profile of HD-HIV cells was then compared to HUT78-HIV cells The analysis ... hematopoietic cells Our current findings clearly demonstrate a profound increase in the susceptibility of HD cells to HIV-1 infection Interestingly, higher levels of p24 expression were observed in 8-HD...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
... neural stem cells and Schwann Cells results in functional recovery in a rat spinal cord contusion injury model Biomed Environ Sci 2007, 20:242-249 Lindvall O, Kokaia Z, Martinez-Serrano A: Stem cell ... S, Weidner N, Winkler J: Adult stem cell therapy in stroke Curr Opin Neurol 2005, 18:59-64 Li M, Decherchi P, Raisman G: Transplantation of Olfactory Ensheathing Cells into Spinal Cord Lesions ... (MHC) in neurons, but not in glial cells, and decrease the expression of the class II MHC in glias [4] Immune response and inflammation are common sources of secondary injury in neural cells...
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Báo cáo sinh học: "Compound developmental eye disorders following inactivation of TGF signaling in neural-crest stem cells" doc
... mapping to define in detail the contribution of the NC to the forming eye in mice In addition, we used conditional gene targeting to inactivate TGF signaling in NC stem cells and, as a result, in ... anomaly, is TGF -dependent, suggesting that both transcription factors are involved in mediating TGF signaling in ocular cells during development Interestingly, a report of a family suffering from ... of NC -derived cells into collagen-synthesizing stromal keratocytes Moreover, in forming corneal endothelial cells (and in the TM), expression of Foxc1 and cell survival requires TGF signaling...
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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
... hES-CD34+ cells whereas studies of Galic et al [20] derived functional T cells from hES-CD34+ cells in vivo using humanized mice Whether the hES derived T cells support HIV-1 infection remains to ... cells into DCs The undifferentiated hES cells (H1) were harvested by treatment with mg/ml collagenase IV (Invitrogen) and dispersed by scraping to maintain the cells in small clumps The hES cells ... target cells that need to be protected against HIV-1 infection Methods hES cell growth and propagation H1 human embryonic stem cell line (hES) was obtained from WiCell (Madison, Wisconsin) The...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
... triangles-kinase, circles-others) A solid line indicates a direct interaction while a dashed line indicates an indirect interaction A line without an arrowhead indicates binding and a plus sign indicates ... solid line indicates a direct interaction while a dashed line indicates an indirect interaction A line without an arrowhead indicates binding and a plus sign indicates that othernetworks contain ... comparing BCs to leukocytes identified genes involved in vasculogenesis, to endothelial cells identified genes involved in hematopoiesis, and to stromal cells identified genes involved in heart...
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Exploring mesenchymal stem cell derived exosome and tocotrienol (t3) as therapeutic agents in drug induced liver injury (DILI)
... on APAP and H2O2-induced on cell death in TAMH cells 112 4.3.2 Effect of lower dosage of -T3 and -T3 against APAP and H2O2-induced injury on cell viability in TAMH cells 118 4.3.3 ... APAP- and H2O2-induced liver injury in TAMH cells 156 Figure 44 Combination therapy of exosomes and -T3 in APAP- and H2O2induced liver injury in TAMH cells 160 xvii List of ... TFF TGF- TNF- TNFR1 TP Interleukin Inducible nitric oxide synthase Insulin, transferrin, selenium c-Jun-N-terminal kinase Liver function test Lipid peroxidation Mitogen activated protein kinase...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... 1.2.1.1 Inhibiting the TGF- β signaling pathway enhances neural induction The transforming growth factor beta (TGF- β) signaling pathway is involved in many cellular processes, including cellular differentiation ... ReN-VM cells was performed every to days, by dissociating into single cells following incubation with Accutase for minutes, collecting cells by centrifugation at 1000 rpm for minutes and replating ... mesencephalon -derived neural stem cells (ReN-VM) 3.6.1 Maintenance of ReN-VM cells ReN-VM neural stem cells were purchased from ReNeuron (Millipore) and were maintained in NP medium described in Section...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... target 226 lincRNAs expressed in mESCs, and the authors identified 26 lincRNAs that resulted in loss of Nanog and Oct4 expression upon lincRNA knockdown, indicating that lincRNAs maintain the pluripotent ... functional in maintaining pluripotency, their expression would be specific to pluripotent cells Thus, the expression levels of the 16 lncRNAs in undifferentiated human pluripotent stem cells and ... governing stem cell pluripotency and cell fate determination, and perturbations of lncRNA levels will result in stem cell differentiation 104 Chapter VII – Long Non-coding RNAs are Indispensable in...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation ... qPCR in two independent cell lines: hESC (H1) -derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC -derived cells and ReN-VM cells, RMST expression was upregulated by approximately ... database indicates the presence of a REST binding site upstream of RMST REST (or NRSF) binding sites were observed upstream of RMST in three human cell lines, namely GM12878, a lymphoblastoid cell line,...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
... Liu, Y., Shin, S., Zhan, M., Rao, M.S., and Zeng, X (2010) Stage-specific role for shh in dopaminergic differentiation of human embryonic stem cells induced by stromal cells Stem Cells Dev 19, ... extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic stem cells (hESCs), and human neuronal development, using hESCs as a cellular ... and Freed, W.J (2008) Assessment of stromal -derived inducing activity in the generation of dopaminergic neurons from human embryonic stem cells Stem Cells 26, 1517-1525 Walker, E., Chang, W.Y.,...
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Dual roles of transcription factor zic3 in regulating embryonic stem cell pluripotency and differentiation
... Introduction 1.1 Derivation of embryonic stem cells 1.2 Regulation of embryonic stem cells 1.2.1 The key properties of ES cells 1.2.2 Extrinsic signalling pathways maintaining ES cell ... 4: Zic3 interacts with Sox2 in ES cells 124 4.1 Introduction 4.2 Results 4.2.1 Zic3 interacts with Sox2 in embryonic stem cells 4.2.2 Zic3 shares regulatory pathways with Sox2 in ES cells ... following sections 1.2.2 Extrinsic signalling pathways maintaining ES cell pluripotency Embryonic stem cells are maintained by a network of extrinsic and intrinsic signals that collectively regulate...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
... anchoring substrates are the underlying principles of these systems, which try to mimic the microenvironments of the hESCs Indeed, keeping in mind the origin of hESCs, the neighbouring cells ... generating new epithelium and basement membrane, providing anchorage for the hESCs [18] The presence of ECM proteins in developing embryos indicates that these proteins play a role in maintaining ... LAP binds to TGF in its latent form, and the latter becomes activated when released from LAP [31, 32] TGF prevents ES cell differentiation by maintaining Smad2/3 phosphorylation [33] FGF-2 interacts...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
... 105340 Keratin, type I cytoskeletal 10 Keratin, type II cytoskeletal Collagen alpha-1(I) chain*† Collagen alpha-2(I) chain*† EMILIN-1*† Fibrillin-1* Isoform of Collagen alpha-1(XII) chain Isoform ... metalloproteinase with thrombospondin motifs 1*† Transforming growth factor-beta-induced protein ig-h3 74665 122841 234698 Isoform of Extended synaptotagmin-1*† Isoform of Myoferlin*† 99.80% ... alpha-2(VI) chain Thrombospondin-1* Vitronectin* 100.00% 12.40% Isoform of Tenascin*† Isoform of Transglutaminase 100.00% 10.50% 99.80% 4.61% 100.00% 99.80% 8.29% 8.79% Thrombospondin-1* 100.00%...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
... aid in increasing integrin binding and hence cell binding This contradiction could possibly be due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering ... interfering with TGFB signaling for the hESCs, since DCN and BGN both bind active TGFB, and could lead to a decrease in the amount of active TGFB available for the hESCs 3.5 Matrices that maintain ... maintain hESCs in a pluripotent state This observation is surprising, since these matrices contains more Fn (by immunofluoresence comparison, section 3.3.2), it would be expected that the 52 increased...
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