tfiiia and p43 binding to 5s ribosomal rna
Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-affinity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt
... contributions to the exposure and positioning of the Tyr304 residue for binding to tyrosine kinases and phosphatases On the other hand, tyrosine phosphorylations not affect the conformation of the PDZ binding ... cells and tissues into defined functional aggregates and compartments Acknowledgements We thank Drs Dmitri Tolkachev and Surajit Bhattacharjya for their valuable discussion and Patrice Bouchard and ... therefore interesting to know whether Grb4 and PDZ-RGS3 can bind to the cytoplasmic tail of ephrin B2 simultaneously or whether Grb4 binding can affect or even exclude the binding of the PDZ-RGS3...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... uorescence and acceptor absorption, and j2 is the orientation factor and accounts for relative orientation of the donor emission and acceptor absorption transition dipole Generally, j2 is assumed to ... strand was added, and the mixture was heated for at 96 C and slowly cooled to room temperature The double-stranded DNA was stored at )20 C in experimental buffer Protein purication The tryptophans ... binding to the syn-cAMP -binding sites at concentration of the ligand of mM causes a % 6% increase in its uorescence intensity, which indicates that this residue is sensitive to cAMP binding to...
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Báo cáo khoa học: Thermodynamic characterization of substrate and inhibitor binding to Trypanosoma brucei 6-phosphogluconate dehydrogenase pot
... enthalpy change for NADP binding is relatively low, and a positive entropy change contributes to binding For NADPH and aPyADP, the binding appears to be totally enthalpic, and a negative entropy ... data for the binding of aPyADP to the enzyme are shown in Fig The binding stoichiometry was close to two Table Binding parameters of coenzymes to 6PGDH from Trypanosoma brucei Ligand Kd (lM) DH0 ... per se To better understand why these analogues have high affinity and to help in rational drug design, we undertook a thermodynamic characterization of substrate and analogue binding to T brucei...
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Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
... resonance study of the cyclic AMP receptor protein (CRP): assignments of the NH protons of histidine and tryptophan residues and the effect of binding of cAMP to CRP Biochem J 107, 304309 14 Sixl ... absorbance at both 280 and 340 nm were combined and dialyzed extensively against buffer B The stoichiometry of labeling was determined spectrophotometrically and ranged from 1.0 to 1.8 mol of the ... and various concentrations of the labeled protein, from to lm Five to 10 kinetic traces were collected and averaged for each concentration point The data were tted to extract rate constants and...
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Báo cáo khoa học: Modulation of heme and myristate binding to human serum albumin by anti-HIV drugs An optical and NMR spectroscopic study potx
... online: Fig S1 Binding isotherms for abacavir (A) and nevirapine (B) binding to HSA (FA free), at pH 7.0 and 25°C Fig S2 Binding isotherms for Fe(III)heme binding to HSA (FA free) and to HSA–myristate ... Fanali et al Fig Binding isotherms for Fe(III)heme binding to HSA (FA free) and to HSA–myristate complexes in the absence of drugs (A) and presence of abacavir (B), nevirapine (C) and atazanavir ... effectors, HSA changes conformation to the basic (B) form (neutral -to- basic, N fi B transition) with loss of the a -helix content and an increased affinity for some ligands [1,6,7,30–37] Ligand binding...
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Báo cáo y học: "Selective translational repression of HIV-1 RNA by Sam68DeltaC occurs by altering PABP1 binding to unspliced viral RNA" pps
... PABP1 binding to US-C pgTat mRNA but did significantly increase PABP1 binding to the kb HxBruR-/RI- mRNA In contrast, Sam68ΔCmin was able to block expression of pgTat RNA but not the kb and kb RNAs ... Sam68ΔC mutants Total RNA was harvested and used in the assays below a) 20 μg of total RNA was selected using oligo(dT) beads and both the polyA- and polyA+ fractions were input into the RNase protection ... proteins with pre-mRNA and mRNA: candidate intermediates in formation and export of mRNA Mol Cell Biol 2001, 21(21):7307-7319 Kindler S, Wang H, Richter D, Tiedge H: RNA transport and local control...
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Báo cáo khoa học: pyr RNA binding to the Bacillus caldolyticus PyrR attenuation protein – characterization and regulation by uridine and guanosine nucleotides potx
... 2A–C) Binding was specific for pyr RNA, as shown by the failure of a control RNA (i.e the antisense strand to BcBL1) to bind to any concentration of PyrR tested (Fig 2A) Binding of PyrR to BcBL2 and ... all three binding loops, RNA binding to PyrR PyrR binding to BcBL1 resembled the binding observed for the other two binding loops The apparent dissociation constant (Kd) values for RNA binding ... Complexity of RNA binding to PyrR The complex binding curves for BcBL1 and for all three binding loop RNAs in the presence of guanosine nucleotides (Fig 2) indicate that the binding of RNA to PyrR...
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Báo cáo y học: " PRMT6 diminishes HIV-1 Rev binding to and export of viral RNA" pps
... CAT compared to R35K (57%) and R39K (32%), although R38K is not thought to be a main actor in binding to the RRE [16] This clearly shows that small changes can be very detrimental to good Rev-RRE ... reduced RRE binding and diminished export of viral RNA to the cytoplasm in cell-based assays Coimmunoprecipitation experiments confirmed the association of PRMT6 with Rev, which was shown to undergo ... cells expressing siRNA against PRMT6 were used RNA was isolated for reverse transcription and mean Rev amounts determined by rt-RT-PCR were normalized to GAPDH (left panel) or total RNA (right panel)...
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Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
... that the observed heterogeneity in binding of [125I]TC-PCSK9 to HepG2 cells is attributable to binding to different populations of LDLR that exist prior to ligand binding (see below) Previous Biacore ... which the first binding step, representing binding to the EGF-A domain, is followed by binding of PCSK9 to a second site within the receptor In this model, dimeric and monomeric receptor states bind ... density and high density lipoprotein receptors in the rat corpus luteum and regulation by gonadotropin J Biol Chem 258, 8020–8027 Taylor SI (1975) Binding of hormones to receptors An alternative...
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Tài liệu Báo cáo khoa học: Calcium-binding to lens bB2- and bA3-crystallins suggests that all b-crystallins are calcium-binding proteins pptx
... bind to the calcium -binding sites in proteins and induce luminescence peaks at 492 nm and 547 nm via energy transfer from Trp and Tyr residues [32] Terbium binds to bB2- and bA3-crystallins and ... All lens b-crystallins are calcium -binding proteins M K Jobby and Y Sharma Table Binding constants and the enthalpy change of calciumand terbium -binding to bB2- and bA3-crystallins K, dissociation ... terbium binding to this crystallin by ITC and determined the binding constant for the calcium mimic probe Terbium is believed to bind strongly to calcium -binding sites of proteins compared to calcium...
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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt
... every atom, in every residue, as a unique type (e.g alanine Cb and arginine Cb are considered as unique atom types under this scheme), resulting in a total of 158 protein, ˚ and 81 RNA atom types ... protein RNA structures This kind of method was proposed by Sipple [20], and has been applied to protein structure prediction, protein–protein and protein–ligand interactions [18–33], as well as to ... of RNA recognition for RRM proteins [6,39,40] and by the structure of existing KH domains bound to RNA [6,41–44] As a consequence of the assumptions of the model, complexes containing two RNA- binding...
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Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx
... affinity to HSA, whereas warfarin and FA7 ligands were reported to behave in the opposite way with respect to ferric heme binding to HSA [19,21,31] Additionally, the affinity of Mn(III)heme for HSA and ... Mn(III)heme and myristate for binding to FA1 (defined by the parameters KH and KM) and on the allosteric modulation of FA1 properties by myristate binding to FAx (defined by the parameters KM*, K H M , and ... Myristate and Mn(III)heme binding to HSA–heme G Fanali et al Table Values of the thermodynamic dissociation constants (M) for myristate and Mn(III)heme binding to HSA at pH 7.0 and 10.0 (Scheme and...
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Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx
... unidentified ligand in red cells that could mediate binding to CD11b and probably also to CD11a CD11b I domain inhibits red cell binding to purified integrin Red cells bind poorly to CD11a/CD18 ... enhancement, suppression, and modification of ligand binding activity We have previously shown that Ca2+ and Mg2+ are needed for the maximal binding of CD11a/CD18 and CD11b/ CD18 to ICAM-4 [14,28] Here ... soluble competitor proteins and EDTA on binding of recombinant I domain GST to coated ICAM4Fc in a solid phase assay CD11a I domain GST (A) and CD11b I domain GST (B) binding to ICAM-4Fc was...
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Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... increased binding to SM3 [18] As there is no published X-ray structure of a glycopeptide binding to SM3 it is not known how a sugar moiety contributes to binding energy and whether peptide and glycopeptide ... corresponds to a ligand to protein ratio of 20 : For STD experiments the ligand to protein ratio was raised to 150 : (2.88 lmol, 4.8 mM PDT(O-a-D-GalNAc)RP) Peptide or glycopeptide were added to the ... b protons Assuming a binding mode as found in the X-ray structure analysis the closest distance of a protein ˚ proton to the Asp2-b/b¢ proton is 2.7 and 3.4 A, respectively This relay proton contributes...
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Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc
... drug, and ibuprofen, a nonsteroidal anti-inammatory drug, are considered to be stereotypical ligands for Sudlow sites I and II, respectively In contrast to warfarin, ibuprofen has been reported to ... binding to the secondary sites FA2 and FA6 rather than drug association with the FA3 FA4 primary cleft Results Ibuprofen binding to tHSAheme-Fe(III) and HSAheme-Fe(III)] Figure 2A shows the binding ... (1.7 ã 10)5 and 8.9 ã 10)4 m, respectively) and to HSAheme-Fe(III) (6.9 ã 10)6 and 8.1 ã 10)4 m, respectively) The spectroscopic contributions of ibuprofen binding to the high-afnity and low-afnity...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... peptide -binding groove that mediates antigen binding and presentation to CD8 + cytolytic T cells In HFE, the proximity of the two a helices and the presence of amino acid side chains that project into ... HFEC282Y variant fails to make it to the cell surface and is unable to exert this control We wanted to determine FEBS Journal 277 (2010) 3219–3234 ª 2010 The Authors Journal compilation ª 2010 ... 61129/2004) and the Health Research Board (RP/ 2006/294) to J.V.F and Health Research Board Grant (RP/2005/107) and Science Foundation Ireland (SFI) Investigator Grant (05/IN.3/13859) to MMC The...
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Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx
... C2, EF-hand mutations and increased the KD by about a factor of seven (to 1560 ± 190 nm compared to 223 ± 44 nm for the wild type); EF-hand mutations and resulted in a complete loss of binding ... Upon binding of Ca2+ to the C-lobe, CaM undergoes a first conformational change, exposing a hydrophobic binding pocket and, hence, promoting binding to the target (e.g [13,16,17]) Binding to the ... threshold (Fig 2) and by assuming that a CaM binding domain has to have a length of at least around 15 residues For quantitative estimates of the binding of CaM and CaM mutants to peptides and fusion...
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Báo cáo khoa học: Actin-binding domain of mouse plectin Crystal structure and binding to vimentin pot
... strong binding of plectin ABD to full-length vimentin and VimNR, but only weak or no binding to other vimentin fragments To confirm the specificity of the plectin ABD–vimentin interaction and to more ... that binding occurred to IF subunit proteins in their nonfilamentous state [17–20] Fimbrin was unable to bind to polymerized vimentin in cosedimentation assays and binding occurred at a stoichiometry ... structures I and II is stable and not subject to conformational changes due to different crystal packing Quality of protein structure models The final R and Rfree factors for structure I were 15.3 and...
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Báo cáo khoa học: Binding of the volatile general anesthetics halothane and isoflurane to a mammalian b-barrel protein doc
... the data to a single-site binding model 575 General anesthetic binding to a b-barrel protein J S Johansson et al Fig Competition between halothane and 1-aminoanthracene (AMA) for binding to porcine ... anesthetics can bind to a-helical proteins [5–12,27,28], but this is the first study to demonstrate binding to a b-barrel protein using direct binding assays Isoflurane has been shown to bind to bovine serum ... energy of binding (DG°) exceeded the heat of binding (DH°) by more than a factor of two (Table 1), indicating that binding to porcine odorant binding protein is entropy driven, in contrast to the...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... atoms Number of protein atoms Number of waters Number of inhibitor atoms Baverage (A2) Protein main chain atoms Protein side chain atoms All protein atoms Inhibitor atoms Water atoms Other atoms ... proteinligand complex, and [P] and [L] are the concentrations of the free protein and free ligand (i.e inhibitor), respectively [68] Expressed in terms of r, dened as the number of ligands bound to ... 2-methyl-2,4FEBS Journal 272 (2005) 23172333 ê 2005 FEBS J Janis et al ă Thioxylo-oligosaccharide binding to xylanases Table Hydrogen bonds formed between CTX xylanase and S-Xyl5-Me inhibitor Inhibitor atoma...
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