... predominant transcription start site and several other transcription start sites inthe mouse CB1 gene that were used to express the CB1 gene in striatal and cortical neurons We consistently saw the ... transcript was 0.1 in all cortical and striatal RNA samples suggesting that there was decreased transcription ofthe CB1 gene in HD mouse brain This supports the hypothesis thatthe rate of ... regions ofthe mouse and human CB1 genes demonstrated that there were a number of transcription factor binding sites that have been conserved between thetwo species, suggesting that some common individual...
... with radio s insistence on inoffensiveness andthe audience s desire for soothing sounds Such bands also controlled the best remaining ‘location jobs’ in hotels and cafes.” By the summer of 1935, ... all across the country To contemporary ears, the music ofthe “sweet” bands sounds hopelessly predictable, andthe bands seem barely distinguishable from one another The sentimental sounds that ... project, the documentation ofthe life and music ofthe Mississippi Delta blues musician Robert Johnson; it was through his efforts thatthe copyright status of Johnson s music and photographs is now...
... undergrowth inthe study area The plant species ofthe FA vegetation band are dominant, further the species ofthe QTA and QRC vegetation bands, possibly also the species belonging to two vegetation bands ... combination of sections of “guide” series, being determined by different species (in the first place tree or shrub determinants!) ofthe main level synusia of natural forest and shrub geobiocenoses and ... hills, inthe foothills ofthe Nízký Jeseník hills, inthe Zábřežská vrchovina hills, inthe Moravská brána, inthe lower parts ofthe Podbeskydská pahorkatina hills andthe Opavská pahorkatina...
... with the said Yet Levinas goes a step further He personalizes the speaking language in terms which sound rather unusual inthe ears of Saussurian linguistics.9 Sign systems consist of signs, splitting ... whose otherness is at stake The otherness does not lie behind the surface of somebody we see, hear, touch and violate It is just his or her otherness It is theother as such and not some aspect ... is not physical, but ethical Theother resists violence not as somebody belonging to the totality of beings, but as an infinite which is beyond all we can to theotherThe otherness ofthe other...
... Grb14–IR interaction The PIR–BPS binds as a pseudosubstrate inhibitor inthe substratebinding groove ofthe kinase, whereas the SH2 domain interacts with phosphorylated tyrosine residues ofthe IR kinase ... overexpression of Grb14 in CHO-IR cells impairs Akt and ERK insulin signaling pathways and inhibits distal effects of insulin on glycogen and DNA synthesis [6–9], and microinjection of Grb14 into ... opposite effect To address this question, we first assessed the ability of insulin administered in vivo to increase the level of RCAM-lysozyme in cell fractions, and then assayed the fractions of...
... biological signi®cance ofthe different regulation of their expression As a ®rst element step towards understanding, a recent demonstration indicated thatthe synthesis of cell surface HSPG and FSH-stimulated ... involvement of cell HSPG inthe agerelated increase in Sertoli cell phosphodiesterase activity andinthe concomitent loss of steroidogenic response to FSH These data and our results suggest that bFGF could ... (5%), estradiol 17a (0.28%), estrone and estriol (0.45%) The sensitivity ofthe assay was pg per tube Intra- and interassay coef®cients of variation were less than 10% The analysis ofthe radioimmunoassay...
... 6ị In this instrumental setup, G is a scaling factor which is independent ofthe emission wavelength and, in general, has values near It takes in account small instabilities ofthe laser and ... was determined by global analysis ofthetwo polarized components ofthe uorescence intensity, IVV(t) and IVH(t), as well as Im(t), using the same routines The analysis consisted in nding the ... taken into account First, there are several tyrosine residues in RepA As the uorescence was excited at 280 nm, there is the possibility that some ofthe ve tyrosines present inthe W94-containing...
... treatment The purified SH was dissolved in 50 mM Tris/HCl pH 8.0 and stored in liquid nitrogen Unless specified otherwise, this buffer was used in all experiments The purity ofthe samples was examined ... gigas enzyme is missing in HoxY ofthe SH, suggesting an exposure of part ofthe hydrophobic surface ofthe HoxH subunit It was therefore proposed thatthe HoxU subunit ofthe NADH-dehydrogenase ... andthat FMN-a is essential for the NADH-induced activation ofthe Ni-Fe site inthe SH, as well as for the H2-NAD+ reaction Conclusions The SH contains two FMN groups [26,37] The experiments...
... very similar pattern of five bands, oneofwhich was also more intensive than the others Thus, the different intensity ofthe bands may bethe result of a different copy number ofthe genes inthe ... modifications cause the differences between the proteins [15] The present work describes the full-size encoding sequences ofthe mistletoe toxic lectin genes Analysis ofthe primary structure ofthe ... acids linker, and 216 amino acids ofthe B-chain ofthe ml gene was obtained The sequence was different from that obtained using degenerate primers The obtained sequence data were used to design...
... Taken together, these data strongly suggest that lipid 004–1 is a GPI intermediate containing a single mannose substituted with a side-branching EthN-P residue, and corresponds to GPI species ... assembly pathway Sequence analysis ofthe Ybr004cp protein family Database searches using theS cerevisiae Ybr004cp protein sequence andthe Psi-BLAST algorithm revealed 25 similar sequences in ... addition of Man2 to GPI precursors Epistasis tests place Ybr004cp inthe GPI biosynthetic pathway To obtain genetic evidence that YBR004c functions inthe GPI biosynthetic pathway, the epistasis relationships...
... rough and smooth LPSs of E coli andS minnesota inthe presence of EDTA revealed that both inthe presence and absence of EDTA, the oxidation of crosslinked Hb was faster inthe presence ofthe smooth ... coli LPSs were more effective than theS minnesota LPSs in increasing the rate of oxidation, suggesting a difference in binding ofthetwo types of LPSs The effect ofthe LPSs on the rate of oxidation ... possibility of dissociation ofthe Hb A0 into dimers, which is not possible for the cross-linked Hb, as cross-linking the a chains prevents dissociation into ab dimers Dissociation into dimers is known...
... the proteinases of G lamblia and G gallus All Cys and Trp residues are absolutely conserved in a-expansins and b-expansins as well as inthe C1 cysteine proteinases Other highly conserved amino ... distribution of cysteine residues is almost identical between cathepsins and expansins The prose- Ó FEBS 2002 quences of modern cathepsin B proteinases share a critical (inhibitory) cysteine residue in ... similarities between expansins and cathepsin (Q10834, 1.9e)7) as well as other cysteine proteinases (Q40261, 4.2e)6) Additional cathepsin B-like cysteine proteinases as well as cysteine proteinases from...
... determined according to the bicinchoninic acid method [20] with BSA as standard RESULTS Synthesis, purification and analysis of wild-type and mutant oxaloacetate decarboxylases in E coli To synthesize ... (data not shown) The synthesis of stable decarboxylase complexes containing the three subunits a, b and c was verified for all mutants after affinity purification by SDS/PAGE A selection of these ... binding ligand Sodium ions may still bind to the N373L mutant through coordination to theother ligands ofthe binding site (center I), but the binding becomes much weaker as emphasized by the...
... variations in speed and metabolic parameters within the test Statistical analysis All data is expressed as mean Ϯ SE The reliability ofthe parameters obtained from the 6-MWT was assessed using several ... Nevertheless, its intensity is always greater than the individual ventilatory threshold Theuseof CV and SD in addition to classical statistical analysis has been recommended to study a method s ... similar intwo others (14,19) Thus, this discussion underscores thatthe discrepancy between all 6-MWT distances published is multifactorial To the best of our knowledge, this study is the first to...
... current and future state ofthe U .S nursing workforce, which speaks to a short -term easing ofthe nursing shortage in some parts ofthe country and points to the current recession as a contributing ... expertise inthe education and professional development of nurses worldwide Its membership comprises national associations of nursing deans and schools of nursing and is able to offer information, support, ... compiling workforce research and increasing the supply of nurses andother health professionals, Australia is facing an over-supply of graduates HWA recognizes that this is a temporary issue, due in...
... miles off the coast Gaza has two crossings that allow pedestrians to leave the territory, Erez crossing into Israel and Rafah crossing into Egypt While tens of thousands of Palestinians once crossed ... its funds and supplies The internal conflict means that communication between thetwo ministries is poor For instance, mistrust between the ministries delays the approval of requests and results ... husband is a fisherman He lost his brother at the end ofthe war Thetwoof them were out at sea fishing inthe final days ofthe war My husband s brother was shot dead by Israeli soldiers He had four...
... complicated because ofthe differentiations of class and status, with some roles becoming dominant (kings and priests and warriors) and others subordinate (wives and servants and slaves) Ruling classes ... focuses on museum art, and aesthetics text on the processes of making, judging, and classifying art But the aesthetics of human wit involve us all in a universal process of appreciative interest ... ascertain about our earliest ancestors, then, human wit theuseof aesthesis in sensory awareness of things leading to capacious discernment of things—has become our agency in dealing with disappearing...
... show the composition of H6fhx1 [1] The disulfide linkage between H- and L-chains is not formed in Nd -s and Nd-sD mutants because ofthe lack of Cys-172 inthe mutant L-chain [8] It is of interest ... important The clue to the first event has been obtained from studies [8] on the naked-pupa mutants, Nd -s and Nd-sD In these mutant silkworms, chimeric L-chains are formed because ofthe exon-shuffling ... these experiments, a large excess of cocoon protein samples was used from Nd-sD andthe transgenic line L7-4 in order to obtain similar signal intensity In all the cocoon protein samples, H-chain...
... increasing the proportion of EU support in order to mitigate some ofthe problems in terms of Member State affordability In terms ofthe relationship between inputs and outputs, evidence suggests that ... offered by the EU to help with the recovery of demand following a crisis, and awareness ofthe availability of these measures among Member States andthe industry should be raised The Commission ... respect to other meat sectors and food safety issues suggesting that, in general, media reporting of food safety issues is a causal factor in subsequent decreases in consumption Our interviews...
... approach, andthe cloned PCR products were checked by sequencing Table shows the primers, vectors, and restriction sites used inthe cloning strategy, and Table lists the primer sequences Constructs ... [18], inthe presence of 50 mM sodium tartrate pH 4.0 at 30 °C (standard conditions) For the stability to the pH or the optimal pH determination, syringaldazine (17 lM) was also used as the substrate ... demonstrated, the presence oftwo isozymes, LacI and LacII, inthe white-rot fungus Pycnoporus cinnabarinus strain ss3, which is the monokaryotic strain derived from the dikaryotic parental strain I-937...