targeted mass spectrometry based metabolomic profiling through multiple reaction monitoring of liver and other biological matrices
báo cáo khoa học: "Applying mass spectrometry based proteomic technology to advance the understanding of multiple myeloma" ppt
Ngày tải lên :
10/08/2014, 22:21
... Ni AP, Chen WM, Xu Y: Construction of a multiple myeloma diagnostic model by magnetic bead -based MALDI-TOF mass spectrometry of serum and pattern recognition software Anat Rec (Hoboken) 2009, 292:604-610 ... the other hand, offer a robust, high throughput method that overcomes many of these limitations [36] Most important to cancer research, mass spectrometry can be employed to identify known and ... identification of biomarkers that can be used in diagnosis, prognosis, and treatment assessment Mass Spectrometry A mass spectrometer determines the mass of a molecule by measuring its mass- to-charge...
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báo cáo hóa học:" Mass spectrometry-based serum proteome pattern analysis in molecular diagnostics of early stage breast cancer" pdf
Ngày tải lên :
18/06/2014, 15:20
... sensitivity and accuracy of annotation of m/z values of ions in recorded mass spectra of complex protein mixtures such as biological specimens [20,21] The relevance of mass spectrometry- based serum ... proteomics and mass spectrometry profiling for cancer detection Expert Rev Proteomics 2006, 3:311-320 Dworzanski JP, Snyder AP: Classification and identification of bacteria using mass spectrometry- based ... demonstrating the high potential of mass spectrometry- based analyses of the blood proteome pattern in diagnostics of breast cancer once problems with standardization of experimental and computational design...
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Báo cáo hóa học: " Mass spectrometry-based analysis of therapy-related changes in serum proteome patterns of patients with early-stage breast cancer" pot
Ngày tải lên :
18/06/2014, 16:20
... is the subject of clinical or disease proteomics [4,5] Mass spectrometry- based analysis of the blood proteome is an emerging method of clinical proteomics and cancer diagnostics, and the low-molecular-weight ... responses of patients to therapy but not restoration of the "normal healthy" pattern of the serum profile Conclusions Here we established the high potential of MALDI-ToFbased analyses for detection of ... University of Technology, Gliwice, Poland, 3Polish Academy of Science, Institute of Bioorganic Chemistry, Poznan, Poland and 4Polish-Japanese Institute of Information Technology, Bytom, Poland Received:...
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Báo cáo hóa học: " Mass spectrometry based on a coupled Cooperpair box and nanomechanical resonator system" pdf
Ngày tải lên :
20/06/2014, 22:20
... between the NR and the CPB island, Cg and Vg are, respectively, the gate capacitance and voltage of the CPB qubit, and CJ is the capacitance of each Josephson junction Displacement (by x) of the NR ... adsorption of the DNA molecules because of the increased mass of the NR According to Equation 19, we can obtain the mass of the accreted DNA molecule: m = − 2mneff ω = 16590zg, about the ω mass of 10 ... frequency of the NR by calculating the absorption spectrum [23] On the other hand, NRs have widely been used as mass sensors by measuring the resonant frequency shift because of the added mass of the...
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Báo cáo y học: "Evaluating the potential of a novel oral lesion exudate collection method coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery" docx
Ngày tải lên :
13/08/2014, 13:20
... Chromatography and Tandem Mass Spectrometry- Based Identification of Potential Biomarkers of Oral Epithelial Dysplasia and Novel Networks between Inflammation and Premalignancy Journal of Proteome ... Discovery and verification of head -and- neck pre-cancer and cancer biomarkers by differential protein expression analysis using iTRAQ-labeling and multidimensional liquid chromatography and tandem mass ... Discovery and verification of head -and- neck cancer biomarkers by differential protein expression analysis using iTRAQ labeling, multidimensional liquid chromatography, and tandem mass spectrometry...
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CHARACTERIZING ROLES OF ANNEXIN 1 IN BREAST CANCER DEVELOPMENT BY MASS SPECTROMETRY BASED QUANTITATIVE PROTEOMICS
Ngày tải lên :
10/09/2015, 09:04
... limitations of low resolutions and background bias With the advancement of technology and development of informatics, high resolution mass spectrometry (MS) -based proteomics is an uprising high throughput ... proteomics, namely the peptide mass fingerprinting (PMF) and tandem mass spectrometry (tandem MS) In peptide mass fingerprinting, it lies on the basis that if the mass of the peptides could be measured ... methodology of MS -based proteomics involves the use of a wide variety of 17 separation methods with mass spectrometry (MS), which consists of an ion source for volatizing proteins or peptides, a mass...
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Development and application of mass spectrometry based proteomics technologies to decipher ku70 functions
Ngày tải lên :
04/10/2015, 15:46
... different mass analyzers provides a versatile set of mass spectrometry methods with surprising sensitivity and accuracy for protein and other biomolecular analysis The true power of mass spectrometry ... completion of the genome sequences of the human and many other organisms is undoubtedly a big step towards the full understanding of biological sciences The availability of enormous amount of data ... of BSA in other lanes of the same gel The bands were digested and desalted by Empore Disk The bands with one picomole and 500 femtomole of BSA are consistently detected by MALDI-TOF/TOF every...
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Báo cáo khoa học: Multiple-probe analysis of folding and unfolding pathways of human serum albumin docx
Ngày tải lên :
16/03/2014, 16:20
... 400 nm with excitation and emission band pass of nm and nm, respectively ð3Þ DGUÀFH2 O ð4Þ where aF and aU are the intercepts and bF and bU are the slopes of the baselines of the equilibrium fluorescence ... values of m and Dm The curve fittings were performed using MICROSOFT ORIGIN software Calculation of the rate constants from the kinetics of refolding HSA was found to follow two phases of refolding, ... part of the folding occurred through a slow phase The rate constant of the burst phase of the refolding step could not Fig The effect of GdnHCl on the far-UV CD spectra of HSA (A) CD spectra of...
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Báo cáo khoa học: "Paragraph-, word-, and coherence-based approaches to sentence ranking: A comparison of algorithm and human performance" ppt
Ngày tải lên :
17/03/2014, 06:20
... paragraph -based rankings and human rankings only for one of the 15 texts All other algorithms provided scaled importance rankings Many evaluations of scalable sentence ranking algorithms are based ... the weight of each cluster is calculated by dividing the square of the number of significant words in the cluster by the total number of words in the cluster Sentences can contain multiple clusters ... the weight of a sentence, the weights of all clusters in that sentence are added The higher the weight of a sentence, the higher is its ranking A more recent and frequently used word -based method...
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Báo cáo khoa học: "A Unification-based Approach to Morpho-syntactic Parsing of Agglutinative and Other (Highly) Inflectional Languages" doc
Ngày tải lên :
23/03/2014, 19:20
... of handling compounding and adequate handling of derivational affixes Morpheme sequence recognition is achieved through the meta-dictionary Recent implementations of Humor 99 define the set of ... is based on unification, or, strictly speaking, checking unifiability of the adequate features of stems and suffixes A phonologically and ortographically motivated allomorph -based variant of ... descriptions, and classes of surface allomorphs The latter is a cross-classification of the paradigms according to phonological and graphemic properties of the surface forms Both verbal and nominal...
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Báo cáo khoa học: "A Unification-based Approach to Morpho-syntactic Parsing of Agglutinative and Other (Highly) Inflectional Languages" ppt
Ngày tải lên :
23/03/2014, 19:20
... of handling compounding and adequate handling of derivational affixes Morpheme sequence recognition is achieved through the meta-dictionary Recent implementations of Humor 99 define the set of ... is based on unification, or, strictly speaking, checking unifiability of the adequate features of stems and suffixes A phonologically and ortographically motivated allomorph -based variant of ... descriptions, and classes of surface allomorphs The latter is a cross-classification of the paradigms according to phonological and graphemic properties of the surface forms Both verbal and nominal...
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Báo cáo y học: "Systematic detection of putative tumor suppressor genes through the combined use of exome and transcriptome sequencin" ppt
Ngày tải lên :
09/08/2014, 22:23
... chromosomal Page of 14 distribution of the 1,918 candidate genes and the 50 ASE genes is shown in Figure 3b Biological categorization of the 86 ASE genes in HCC1954 shows that many of them are associated ... Of these, 766 (9.7%) are novel A total of 3,086 of the 7,848 SNVs were found in 1,918 genes, each of which was represented by at least 20 transcriptome reads Comparison of SNVs in the exome and ... heterozygous allelic variations residing in 5’ and 3’ UTRs of mRNAs that are not targeted by probes on the exome array Expansion of targeted regions of the exome array to non-CDS exons would provide...
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Báo cáo y học: " Prevalence and correlates of alcohol and other substance use disorders in young adulthood: A population-based study" pdf
Ngày tải lên :
11/08/2014, 17:20
... take account of the number of additional variables Discussion Prevalence of alcohol and other substance use disorders Using population -based data and comprehensive diagnostic assessment based on ... initiation of substance use, and (4) educational factors were studied as correlates of alcohol and other substance use disorders in young adulthood As substance use and the incidence of SUDs generally ... HA, SK and JL contributed to the conception and design of the MEAF study SK and JS also contributed to the conception and design of the baseline survey (Health 2000) JS, AT-H, JP, SIS and TA-S...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
Ngày tải lên :
19/02/2014, 12:20
... different techniques, of which MS and NMR are two of the most powerful Previously, we demonstrated the distinction of chain type and blood-group type (such as Lea/x and Leb/y) of underivatized oligosaccharides ... order to understand post-translational glycosylation and its function Expansion of our knowledge on the repertoire of carbohydrate structures, the ÔglycomeÕ, and investigation of oligosaccharide ... this report, we demonstrate our strategy of the combined use of ES-MS and NMR for analysis of the core-branching pattern and full sequence assignment of four oligosaccharides isolated from human...
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Báo cáo khoa học: "Determination of Roxithromycin by Liquid Chromatography/ Mass Spectrometry after Multiple-Dose Oral Administration in Broilers" pdf
Ngày tải lên :
07/08/2014, 17:22
... taken at random and killed before the start of the experiment and 0, 1, 3, 5, and 10 days after the last dose Samples of liver, kidney, muscle, adipose tissue and serum were collected and stored in ... and limit of quantitation were based on the signal-to-noise ratio based on their areas The signal-to-noise ratio of was accepted for the limit of detection and that of 10 for the limit of quantiation ... being transferred into other tubes and evaporated to dryness under a stream of nitrogen The residue was reconstituted with ml of methanol and vortexed for 30 s Aliquot of 10 ㎕ was injected after...
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Báo cáo sinh học: "Metabolite-based clustering and visualization of mass spectrometry data using one-dimensional self-organizing map" ppt
Ngày tải lên :
12/08/2014, 17:20
... general, mass spectrometry- based metabolite profiling is performed for each condition with multiple samples For clustering, we use average intensity values of replicas for each marker candidate and ... Figure Illustration of differences between sample -based clustering and metabolite -based clustering Illustration of differences between sample -based clustering and metabolite -based clustering A ... associates the intensity profile of JA and its precursor OPC-4 (blue dot at rt 0.98 in the rt-m/z plane in figure 5) with the profile of a group of marker candidates of high molecular weight (m/z...
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Báo cáo y học: "Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis" pdf
Ngày tải lên :
13/08/2014, 13:20
... with 1.0% FA solution and then again with 50% ACN and 1.0% FA Mass Spectrometry Matrix-assisted laser desorption ionization (MALDI) tandem time -of- flight (TOF/TOF) mass spectrometry was carried ... digested and identified by mass spectrometry Along with the 154 spots, 12 additional spots were selected as prominent features of the gel, and were added to the list of potential proteins of interest ... extraction, in-gel proteolytic digestion and identification of proteins via tandem mass spectrometry analysis Here, we measured the proteomic profile of mesenteric lymph to identify underlying...
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High depth resolution profiling for magnetic sector secondary ion mass spectrometry (SIMS
Ngày tải lên :
16/09/2015, 15:23
... Ion Mass Spectrometry( SIMS) 1.2 Practical issues and solutions to accurate SIMS depth profiling 1.3 Main focus of this thesis Reference for Chapter 1 12 Chapter The Depth Profiling, Modeling and ... 24 W Vandervorst and F R Shepard, J Vac Sci Technol A 5, 313 (1987) 25 R G Wilson, F A Stevie, and C W Magee, Secondary Ion Mass Spectrometry: A Practical Handbook for Depth Profiling and Bulk ... Stevie, and C W Magee, Secondary Ion Mass Spectrometry: A Practical Handbook for Depth Profiling and Bulk Impurity Analysis, (Wiley, New York, 1989), Chaps and 28 G Carter, M J Nobes, F Paton, and...
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Determination of EDTA in Water Samples by SPE-Gas Chromatography/Mass Spectrometry
Ngày tải lên :
05/09/2013, 10:15
... sampling locations other hand, a standard stock solution of CyDTA was prepared by dissolving 0.01 g of trans-1, 2-cyclohexanediaminetetraacetic in 100 mL of M sodium hydroxide Extraction of EDTA from ... Journal of Water and Environment Technology, Vol 8, No.4, 2010 The EDTA standard solution was added to 100 mL Milli-Q water and tap water at concentrations of 0.1 mg/L (1/5 of EDTA standard value ... in 300 mL glass bottles and stored in the dark at 4ºC until analysis Standard and sample preparation A standard stock solution of EDTA was prepared by dissolving 0.127 g of ethylenediaminetetraacetic...
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Tài liệu Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry pdf
Ngày tải lên :
14/02/2014, 08:20
... chemical reactions were monitored in real-time: a one-step Michael addition reaction of phenylethylamine (PEA) and acrylonitrile in ethanol, and a multiple- step acetylation reaction of benzyl ... chemical reactions using EESI-MS Figure Mass spectra of the Michael addition reaction recorded at 20, 60 and 300 min, respectively Inserts: molecular structures of PEA, PEAP and CPEAP 2995 (PAs) and ... on-line monitoring of chemical reactions in industry, providing instant data for the feedback loop to correct possible reaction deviations In addition to real-time monitoring, tandem mass spectrometry...
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