... experiments (sequence 11 4 22 5) Two peptides belonging both to the 11 4 22 5 sequence and exposed regions of subdomain were tested (11 2 – 12 5 and 11 9 – 13 2 peptides) Interaction of the 15 9 19 3 fragment with ... sequences Peptide 15 9 19 3 Kd ELISA Peptide 15 9 19 3 Kd fluorescence Cofilin Kd Reference for cofilin Actin G Actin F 1 10 18 28 84 10 3 11 2 12 5 11 9 13 2 347–365 338–348 360–3 72 355–375 1. 3 mM ND No binding ... was labelled with FITC and mixed with 11 2 12 5 and 11 9– 13 2 actin peptides As shown in Fig 8B, the 11 9 13 2 peptide does not perturb FITC In contrast the 11 2 12 5 peptide induces a fluorescence decrease...
... elegans A 69 gstk -1 (zk1 320 .1) B AA position rGSTK1 GSTK -1 GSTK -2 4 62 51 150 18 2 15 1 gstk -2 (d2 024 .7) E Petit et al 380 599 50 469 10 0 15 0 20 0 C that C elegans ZK1 320 .1 and D2 024 .7 genes belong ... ⁄ VV) at the N-terminus of the GSTK -2 sequence For these reasons, ZK1 320 .1 and D2 024 .7 genes were renamed gstk -1 and gstk -2 50 32 14 7 Fig Genomic structure and intron positions in C elegans gst ... gstk -2( RNAi) gstk -1/ k -2( RNAi) * 18 16 Fig gstk -1 ⁄ gstk -2( RNAi) animals display an abnormal FAME composition Simplified FAME composition of control and gstk -1, gstk -2 and double gstk -1( RNAi) and...