synthesis catabolism and localization
Tài liệu Synthesis Place-and-Route (SP&R) Flow Guide pptx
... BuildGates Synthesis and Cadence PKS s Test Synthesis for Ambit BuildGates Synthesis and Cadence PKS s HDL Modeling for Ambit BuildGates Synthesis s Distributed Processing of Ambit BuildGates Synthesis ... these (and other) PKS commands are provided in the Command Reference for Ambit BuildGates Synthesis and Cadence PKS You can also see Setting Appropriate PKS Controls for Synthesis, Placement, and ... 4.0.8 Synthesis Place -and- Route (SP&R) Flow Guide Synthesis, Placement, and Optimization SPO—Inputs and Outputs Note: The input and output filenames used in this section are for example only, and...
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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... the 58 kD protein band was found in induced sample as the major band, acounted for 80% of total protein (lane 1,3,5 and 6) 58 kD Fig 10 SDS PAGE pattern of PI-17 proteins Mc Standard molecular ... the Future, Michal Lebl and Richard A Houghten (Editors), American Peptide Society, 2001, pp 387-388 Bozenna Rempola, T-Wilusz, W Markiewicz and M.Fkus, Synthesis, cloning and expression in E.coli ... Viktor Kuvshinov and Eija Pehu, Advanced plant Molecular Biology Laboratory Manual, University of Helsinki, Finland, (1998), pp 4-7 Hernandez, J.F., Gagnon, J., Chiche, L., Nguyen, T.M, Andrieu, J.P.,...
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Tài liệu Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx
... previously sequenced RpCAtr (BPNJ ¼ 100 and BPMP ¼ 99) Fungia scutaria (FCA-a and FCA-b) and Caenorhabditis elegans (CA1 and CA2) sequences fall outside of clade I and are more closely related to each ... MC, Andersen AC, Bailly X & Lallier FH (2003) Expression and localization of carbonic anhydrase and ATPases in the symbiotic tubeworm Riftia pachyptila J Exp Biol 206, 399–409 De Cian MC, Andersen ... versus body wall subtracted library (and its reciprocal) and trophosome versus body wall subtracted library (and its reciprocal) For all tissue pairs, cDNA synthesis as well as SSH) [35,36], comprising...
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Tài liệu Báo cáo khoa học: Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulfide bond formation and their contribution to venom complexity pdf
... metalloproteinases and venom complexity J W Fox and S M T Serrano proteins are synthesized in the cytoplasm of secretory cells in the gland and transferred to the rough endoplasmic reticulum, and then ... envenomation and novel toxin-based drug discovery Venom protein biosynthesis Snake venoms are the products of specialized secretory glands located above the upper jawbone in venomous snakes These glands ... venom gland, atrolysin A and VAP1, have their unpaired cysteinyl residues located at positions 360 and 365 respectively, N-terminal to the unpaired cysteinyl residues observed in jararhagin and catrocollastatin...
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Tài liệu Báo cáo khoa học: Caveolin-1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx
... nonspecific band However, immunoreactivity to both the 80 kDa and 60 kDa bands was abolished following preincubation with the antibody control peptide (Fig 1D), implying that the 60 kDa band shared ... SDS ⁄ PAGE and subjected to western blot analysis with antibodies against Caveolin-1, P2X7, PDI, TfR, b-Cop and Flo-1 As expected, Caveolin-1 and Flo-1 were enriched in fractions and 4, representing ... constructs of Caveolin-1 (shRNA1 and shRNA2) and three different scrambled shRNAs (control 1–3), cells were harvested, and the expression levels of Caveolin-1 and of P2X7 receptor were analyzed...
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Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx
... generation and MPO release over fMLP-stimulated levels ARF localization and the guanine nucleotide exchange factors (GEFs) for ARF (BIG-1 and 2), was also found to inhibit O2 À generation and PLD ... above)] and left on ice for 30 Cells were homogenized or briefly sonicated and then spun to remove insoluble material (5 min, 1500 g) The supernatants were collected and respun (100 000 g, 30 min), and ... subcellular distribution of PLD and the consequences of priming and activation were determined using immunoprecipitation and Western blot analysis of neutrophil cytosol and membrane fractions using...
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Báo cáo khoa học: Plasmodium falciparum merozoite surface protein 1 Glycosylation and localization to low-density, detergent-resistant membranes in the parasitized erythrocyte pdf
... been predicted in the Ghana, Png and Uganda sequences The Thr1503 was predicted positive for b (Ghana, Png and Uganda: filled circles, right panel), while Ser1498 and 1506 were positive for a (double ... of Thr1278), and distinguish the two dimorphic forms (Ghana, Png and Uganda vs Thai-K1 and Wellcome) Such b-O-GlcNAc sites are not localized in the regions of homology (387–413 and 1100–1187) ... transferred to nitrocellulose and probed with MEM-43 anti-CD59 mAb (vesicles: WB anti CD59) and revealed by chemiluminescence Ó FEBS 2003 Glycosylation and membrane localization of MSP-1 (Eur J...
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Báo cáo Y học: Novel fish hypothalamic neuropeptide Cloning of a cDNA encoding the precursor polypeptide and identification and localization of the mature peptide pptx
... characterized and subsequently a mature endogenous peptide was identified in the goldfish brain The localization of its transcript in the goldfish brain was further investigated 45 °C and 2.5 at 72 °C, and ... -2 and -3 (see Results and Fig 1)] Brains (n ¼ 200) were boiled for and homogenized in 5% acetic acid as described previously [9,11,14] The homogenate was centrifuged at 15 000 g for 20 at °C and ... (UCSF) and a corresponding peak was further examined in a tandem MS analysis The needle voltage was optimized at 1000 V and the cone voltage was set at 50 V Argon was used as the collision gas and...
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Large scale synthesis, characterization and photoluminescence properties of amorphous silica nanowires by thermal evaporation of silicon monoxide
... crystalline Si core and amorphous SiO2 outer shell structure as confirmed by HRTEM image and SAED pattern On the other hand, the formation of crystalline Si–SiO2 core-shell nanowires first and then complete ... kind of structure as seen by Park and Yong [13] and Zhang et al [23] was observed This reveals that the nanowires have uniform amorphous structure across the length and diameter EDAX spectra of the ... a base pressure of 3.0 Â 10À6 Torr The source SiO and the substrates were inserted in the tube and kept at locations at $1200 1C (center zone) and $1000 1C (in the direction of carrier gas flow),...
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Báo cáo khóa học: Acharan sulfate, the new glycosaminoglycan fromAchatina fulica Bowdich 1822 Structural heterogeneity, metabolic labeling and localization in the body, mucus and the organic shell matrix docx
... body and mucus (Fig 3A,B) correspond to those of chondroitin 4- and 6-sulfate standards (S2 and S3 in Fig 3C, respectively) in this system (molecular mass range of 40–60 kDa) (compare Fig 3A and ... several organs: kidney, salivary glands, esophagus, stomach, albumen gland, feet and spermatheca Light micrographs for stained feet and kidney are shown (Fig 8A; feet and Fig 8B,C; kidney) Radioautographic ... (–) and after (+) papain digestion After enzymatic incubation, the GAGs were applied to polyacrylamide gel and electrophoresis was carried out as described Standard sulfated polysaccharides (Standards)...
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Báo cáo Y học: Characterization of the 105-kDa molecular chaperone Identification, biochemical properties, and localization pptx
... stained with anti-rat 105-kDa protein IgG Panels A and B, cerebral cortex; C and D, hippocampus; E and F, cerebellum Bar indicates 50 lm Fig Localization and expression time of the 105-kDa protein in ... in the panel (#40 and #70) were sequenced by a peptide sequencer B The two purified peptides of lysyl endopeptidase digests (#40 and #70) were sequenced and compared with APG-1 and OSP94 Identical ... was detected in the soluble and insoluble fractions of renal cortex and renal papilla The 105-kDa protein in the kidney is specifically located in the medulla, and the localization of the 105-kDa...
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Báo cáo Y học: Synthesis, characterization and application of two nucleoside triphosphate analogues, GTPcNH2 and GTPcF pdf
... spectroscopic and structural studies was tested by formation of the complex between RasGTPcNH2 and the Ras-binding domain of the Ras effector protein c-Raf-1 MATERIALS AND METHODS General description of synthesis ... 18.2 12.7 19.8 12.4 JPcF – – 936.2 934.0 – – the states A and B and the exchange rates k1 for A fi B and k)1 for B fi A As B ¼ 100%–A and k)1 ¼ k1*A/B only two free parameters had to be fitted to ... spectra (a-, b- and c-phosphorus nuclei), using chemical shift values and J-coupling constants as listed in Tables and Isothermal titration calorimetry The interaction between a nucleotide and the magnesium...
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influence of dopant in the synthesis, characteristics and ammonia sensing behavior
... morphology of the single and co-doped PANI and the direct effects on their conductivity and ammonia gas sensing properties mer, it was filtered and washed several times with methanol and deionized water ... at 1324 cm− and 1296 cm− for PANI_PTSA and PANI_HCl_PTSA respectively can be assigned due to –NH– stretching of secondary amine The bands at 1158 cm− and 1139 cm− for PANI_HCl_PTSA and PANI_PTSA ... Scheme 2.2 Synthesis of processable polyaniline Processable PANI_PTSA and PTSA_HCl_PTSA were synthesized by chemical oxidative polymerization For synthesis of PANI_PTSA, aniline (3.72 g) and PTSA...
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quasi - one - dimensional metal oxide materials — synthesis, properties and applications
... direct band gap of 2.28 eV and an in indirect band gap of 0.55 eV As mentioned before, several synthesis methods to grow Q1D CdO structure have been developed Fig 15a shows a SAED pattern and a ... vapor and oxygen gas The governing mechanisms are the vapor–liquid–solid process (VLS) and vapor–solid process (VS) On the other hand, solution-phase growth methods provide more flexible synthesis ... of precursor and surfactants, and also set the other parameters such as temperature, pH value, and concentration of the reactants As a result, surfactant-assisted system is a trial -and- error based...
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synthesis, characterization and growing mechanism of monodisperse fe3o4 microspheres
... in the number of the reductant and the species of precipitator would influence the synthesis of product 3.1 Effect of reductant and precipitator Fig shows the SEM and TEM images of the product ... 3h and (a)-2 after reaction for 24 h with 0.003 mol ascorbic acid, (a)-3 Fe3O4 standard, (a)-4 Fe2O3 after reaction for 24 h with 0.001 mol ascorbic acid, (a)-5 Fe2O3 standard, (b)-1 FeCO3 standard, ... ascorbic acid, 0.015 g PEG-6000 and 0.4 g NaOH as precipitator a b 50μm μ c 200nm d 200nm Fig SEM images of Fe3O4 products synthesized at 120 1C for (a and b) and for h (c and d) with a solution of...
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synthesis, structure and magnetic properties of iron-doped tungsten oxide nanorods
... temperature and vanishes at 1000 K Among all iron oxides, Fe3O4 and g-Fe2O3 exhibit the largest saturation magnetization of 84 and 74 Am2/kg, respectively The slightly decreasing feature between 800 and ... Science Foundation of China (Grants nos 59725103, 50332020, and 50171070), and the scientific exchange program between China and The Netherlands References [1] A Hoel, L.F Reyes, P Heszler, V Lantto, ... Fe 2p1/2, and Ni 2p photoelectrons in the asprepared nanorods for original surface and surface after argon-ion etching for 150 s, respectively The original surface consists of WO3 and non-stoichiometric...
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Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
... cDNA, and lanes 1, 3, and show the same using genomic DNA of C roseus Primer pairs were: #GSP-4 and #PFLF1 (lanes and 2); #GSP-2 and #GSP-4 (lanes and 4); #GSP-2 and #PFLR-1 (lanes and 6); and ... indicating: floral, vegetative and stress-inducible (abiotic and biotic) expression Information on expression is referenced in Table 1, gathered from published and unpublished sources and from NCBI databases ... in the secondary wall thickenings both in tobacco and in C roseus (Figs 8F and 9D,E) Discussion We report here the cloning, characterization and localization of a novel C roseus peroxidase, CrPrx,...
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Báo cáo khoa học: Sulfated polysaccharides inhibit the catabolism and loss of both large and small proteoglycans in explant cultures of tendon doc
... Catabolism of aggrecan, decorin and biglycan in tendon Biochem J 350, 181–188 Samiric T, Ilic MZ & Handley CJ (2004) Characterization of proteoglycans and their catabolic products in tendon and ... CJ (1996) Catabolism and loss of proteoglycans from cultures of bovine collateral ligament Arch Biochem Biophys 328, 64–72 10 Samiric T, Ilic MZ & Handley CJ (2004) Large aggregating and small ... proteoglycan catabolism in tendon 13 Ilic MZ, Handley CJ, Robinson HC & Mok MT (1992) Mechanism of catabolism of aggrecan by articular cartilage Arch Biochem Biophys 294, 115–122 14 Loulakis P, Shrikhande...
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Báo cáo khoa học: The specificity of alcohol dehydrogenase with cis-retinoids Activity with 11-cis-retinol and localization in retina docx
... all-trans-retinol and all-trans-retinal, and with the cis-isomers of retinol and retinal (7-cis-, 9-cis-, 11-cis- and 13-cis-), were determined for human and mouse ADH1 and ADH4 enzymes (Tables and 3, respectively) ... 11-cis-retinal In all Expression and purification of ADHs Human ADH1B1, ADH1B2, ADH4 and ADH4 M141L, and mouse ADH1 and ADH4 have been expressed at high levels in E coli BL21 cells and purified to homogeneity ... cofactor and substrate concentrations were used: Ó FEBS 2004 1662 S Martras et al (Eur J Biochem 271) 2.4 mM NAD+ (Sigma) and 30 mM ethanol for human ADH1B1 and ADH1B2; 2.4 mM NAD+ and 100 mM...
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Báo cáo khoa học: Methylcitrate synthase from Aspergillus fumigatus Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia ppt
... wild-type strain ATCC46645 and a methylcitrate synthase deletion strain were point inoculated on each plate and incubated for 72 h at 37 °C and photographed Synthesis of cDNA and sequence analysis ... (final concentration) and incubated at 37 °C and 220 r.p.m shaking for indicated times Mycelia were harvested and dried for at least 12 h at 70 °C Dried mycelium was weighed and growth inhibition ... produced and sequenced (Accession No AJ888885) Comparison of genomic and cDNA revealed two introns with a size of 58 and 64 bp Removal of the introns led to an open reading frame of 465 amino acids and...
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