synchrotron based infrared imaging of euglena gracilis single cells
Báo cáo sinh học: "High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry" docx
... from that of the Deiter cells (47.2% ± 4.8 SD), or of the proximal part of the outer pillar cell above the basilar membrane (46.0% ± 5.7 SD), and of one population of tympanic border cells (48.3% ... (h) Quantitation of protein renewal in replicating and non-replicating cells Cy, cytoplasm; NQ, nucleus of non-replicating cells; NR, nucleus of replicating cells The importance of parallel detection ... generation of single cells from the amount of label within the cell, and the possibility of labeling both donor and recipient cells differently, enabling the unambiguous recognition of cell fusion...
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Atomic force microscope imaging of human red blood cells and theri mechanical properties
... 3D view of a fresh RBC 48 Figure 22 Cross section profile of a dehydrated RBC 50 Figure 23 Force curves of A, B, C, D, and E of the dehydrated RBC 51-53 Figure 24 Young’s modulus of points ... modulus of points A, B, C, D, and E of a swollen RBC in PBS solution 59 Figure 28 Cross section profile of a fresh RBC in PBS solution 60 Figure 29 Force curves of A, B, C, D, and E of a fresh ... Figure 34 Cross-section profile of a fresh RBC 69 Figure 35 Cross-section profile of a fresh RBC in PBS solution .70 vii List of Tables Table Young’s modulus of points A, B, C, D, and...
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Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf
... phenotype of single and double deletion mutants The growth phenotype of the yeast strains with deletions of genes encoding various subunits of the bc1 complex was determined by plating the cells ... subunit analysis of single deletion mutants We sought to determine how the absence of individual supernumerary subunits affected the composition of bc1 Table Growth phenotype of single and double ... modification of a previously described method [24] Yeast cells were grown overnight at 30 °C, unless otherwise specified, in 800 mL of YPD until expo3 nential growth phase was reached (D600 of 1–2) Cells...
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Báo cáo Y học: Evaluation of two biosynthetic pathways to d-aminolevulinic acid in Euglena gracilis potx
... second cycle of the TCA cycle, of carbons of 13C-labeled methyl pheophorbide a derived from C-4 of ALA (3) Thus, the 13C-enrichment ratio of C-5 of 13C-labeled ALA (3v-7v) takes the value of between ... C-P16) of the phytyl moiety of 13 C-labeled chlorophyll a were higher than those of carbons of the chlorin ring moiety Synthesis of 13C-labeled methyl pheophorbide a and determination of 13C-incorporation ... See Experimental procedures for details Amount of )1 D-glucose (gáL ) 2.5 10 15 20 Yield (gáL)1) of E gracilis cells No bubbling of air Bubbling of air ± ± 2.11 4.01 4.72 3.64 3.64 4.23 5.85 294...
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Báo cáo hóa học: "Research Article ISAR Imaging of Ship Target with Complex Motion Based on New Approach of Parameters Estimation for Polynomial Phase Signal" docx
... for the received signal of ship target in ISAR imaging is established; in Section 3, the principle of IHMPT is presented, and the ISAR imaging algorithm of ship target based on the IHMPT is discussed ... ISAR imaging based on IHMPT algorithm Table 1: Parameters of the simulated signal Components (k) Ak ak,1 ak,2 ak,3 π/8 × 10−3 × 10−5 1.5 π/4 −1 × 10−3 −6 × 10−5 ISAR Imaging of Ship Target Based ... Data of ξ th range cell Dechirp Output the parameters of all CPSs Approximated as K CPS, k = Estimate αk,3 ^ ξ ≥ M? No ξ =ξ +1 Yes Instantaneous ISAR images based on IHMPT Figure 3: Flow chart of...
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Báo cáo hóa học: " Research Article Single-Channel Talker Localization Based on Discrimination of Acoustic Transfer Functions" pot
... location of the arriving signal [2] As shown in this figure, the use of the GMM of the observed speech had a higher accuracy than that of the mean of the observed speech, and, the use of the GMM of ... comparison of the proposed method using GMM of the acoustic transfer function, a method using GMM of observed speech, that using the cepstral mean of observed speech, and CSP algorithm based on ... GMM of the acoustic transfer Number of mixtures 16 Figure 6: Effect of increasing the number of mixtures in modeling acoustic transfer function, here, 50 words are used for the estimation of the...
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Báo cáo hóa học: " Research Article Active Video Surveillance Based on Stereo and Infrared Imaging" doc
... basis of the results of the previous one The clustering level is composed of a set of classifiers, each corresponding to one of the aforementioned classes of features These classifiers are based ... independent of the distance of the target, will be used for a deeper characterization of the semantic class of the target This distribution represents an additional feature to the classification of the ... length of the movements of the centroid i−2 /n − 1; d= j =i−n C j − C j+1 ∗ // Compute a new point on the basis of temperature similarity criteria in a circular neighborhood Θd of Pi1 of radius...
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Measuring customer based brand equity of english training center
... the value of intangible assets, which is one of the components of the market value of the firm along with tangible assets, brand equity was estimated based on the financial market value of the firm ... equity in the education and training industry, based on the review of CBBE theory, it is argued here that the application of Aaker’s model of customer -based brand equity is the suitable solution ... tangibles as a major source of shareholder value Of these intangibles, brands are often the most valuable assets, accounting for approximately one third of the value of today's Fortune 500 companies...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... bending vibration modes of PVDF [28] The peak at 1200 cm-1 is found to be weak in the PVDF -based composite membranes Blending of styrene is confirmed by the presence of aromatic ring features ... hydrophobic property of PS polymer promoted the formation of these polymer aggregates As can be seen, the thermally induced polymerization of styrene, as well as the blending of PS and SPS polymers, ... uptake of the PVDF membrane decreasing from 22.05% to 14.31%, which can explain the hydrophobic property of PS polymer However, the water uptake of PVDF-SPS membrane is larger than that of the...
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Tài liệu Báo cáo khoa học: Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay docx
... analysis of cRBCs U Aich et al of RBCs occurs when the addition of a limiting amount of virus results in ‘crosslinking’ of RBCs through binding of multiple RBCs to HAs present on a single virus; ... were selected MALDI-TOF/TOF In addition to LC-MS ⁄ MS analysis, we also completed TOF ⁄ TOF analysis of selected peaks to confirm structure using a MDS Sciex 4800 MALDI-TOF ⁄ TOFÔ instrument (Applied ... explorerÔ software TOF ⁄ TOF fragmentation spectra were recorded using negative-ion linear mode with standardized MS ⁄ MS acquisition and processing methods after tuning of the instrument TOF ⁄ TOF spectra...
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Tài liệu Báo cáo khoa học: Seed-based systematic discovery of specific transcription factor target genes pptx
... calculated the ratio of the number of occurrences in the upper 5% of the rank divided by the expected occurrence in the top 5% (given by 0.05 times the total number of occurrences) A list of the motifs ... distribution-distance-derived target prediction based on a seed set of known target genes of a specific transcription factor The target prediction is based on a combination of 3184 5000 10 000 Position in rank ... simplicity of the method, our predictions are very reliable, with 11 of the 16 predictions being true targets, corresponding to an upper bound of the false discovery rate of 33% On the basis of a jack-knife...
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Tài liệu Imaging of the nose and paranasal sinuses pdf
... COMPLICATIONS OF SINUSITIS Orbital cellulitis Subperiosteal abscess Subperiosteal abscess ORBITAL COMPLICATIONS OF SINUSITIS Orbital cellulitis or abscess ORBITAL COMPLICATIONS OF SINUSITIS CT ... COMPLICATIONS OF BACTERIAL SINUSITIS • Periorbital inflammatory edema • Orbital cellulitis • Subperiosteal abscess • Orbital abscess • Cavernous sinus thrombosis ORBITAL COMPLICATIONS OF SINUSITIS ... nasal bones X-rays Plain X rays fracture nasal bones X-rays PLAIN X-RAYS OF THE PARANASAL SINUSES Occipitomental Occipitofrontal NORMAL OCCIPITOMENTAL VIEW (WATER’S VIEW) NORMAL OCCIPITOMENTAL...
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Báo cáo khoa học: Noninvasive imaging of microRNA124a-mediated repression of the chromosome 14 ORF 24 gene during neurogenesis potx
... HT-ori3 cells (normal thyroid cells) , L132 cells (lung normal cells) , C6 cells (glioma cells) , CT-26 cells (colon carcinoma cells) , mesenchymal stem cells (MSCs), and G2 cells (neural stem cells) ... increase of miR124a expression during the neuronal differentiation of P19 cells treated with RA, the endogenous gene expression of five of the candidates was gradually decreased over the period of neuronal ... was transfected into RA-treated P19 cells The Gluc activity of CMV ⁄ Gluc ⁄ c14ofr243¢UTR slightly decreased to the third day of neuronal differentiation of P19 cells (Fig 4D) The significant decrease...
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Báo cáo khoa học: A new clan of CBM families based on bioinformatics of starch-binding domains from families CBM20 and CBM21 potx
... joining of the two CBMs into a common clan Results and Discussion Location of SBD modules in CBM20 and CBM21 With regard to the location of the SBD in the polypeptide chain, analysis of recent ... contains a CBM20 module near the N-terminal end of the protein The N-terminal location is also seen in the case of the majority of unknown proteins of eukaryotic origin with a recognized CBM20 module ... possible to decide the real function of CBM20 in these proteins, with a single remarkable exception, laforin [51], the protein product of the Lafora type of epilepsy gene, which was proven experimentally...
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Báo cáo khoa học: Antibody-based proteomics Analysis of signaling networks using reverse protein arrays pdf
... modulation of regulatory networks [5,6] A direct benefit of such an approach would be the early recognition of ‘off target’ and side effects of drug candidates [7], as well as the identification of putative ... minimum of 1000–2000 molecules present in a single spot, where the total protein content of a single spot corresponds to that of a single cell [9] Cells or tissue samples are subjected to a one-step ... Profiling of kinase inhibitors can be useful to assess the promiscuity of the inhibitor and how much of a problem that might present The first published screens of that kind emphasized the importance of...
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Diversified Funding streams for University-based research: Impact of external project- based research funding on financial management in Universities docx
... He is a member of the executive committee of Humane (Heads of University Administration in Europe) and founding chairman of WSAN He is member of the editorial board of the UK -based higher education ... respective profile and success of the acquisition of project -based funding At the same time, external project -based funding can play the role of a compass with respect to quality assurance of research ... Chancellor of the University of Erlangen-Nuremberg, Germany Gülsün Sağlamer is a Professor of architecture Former Rector of Istanbul Technical University (1996–2004), she is a board member of EUA...
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Iron catalytic growth of prism shaped single crystal silicon nanowires by chemical vapor deposition of silane
... zone axis of single crystal Si Another SAED pattern was taken along [2 1] zone axis of single crystal Si (see Fig 2b, left inset) From the data above, we can confirm the as-grown prism of single ... two-dimensional fourier transform of the outer layer of the triangle (indicated by arrow) depicting lattice spacing of Si(1 1) of 0.31 nm, Si(2 1) of 0.22 nm and Si(2 0) of 0.38 nm, respectively, the ... word, with the assistance of the CNTs and the catalysis of Fe particles, preference of taking the least lattice mismatch between Si and Fe leads to the growth of the single- crystal SiNWs with...
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Infrared spectra of silicon nanowires
... explained from the analysis of oxide layer on SiNWs grown under different conditions The crystalline quality of sample-II is better than that of sample-I because of the improvement of temperature Thus, ... of ∼ 1085 cm− The improvement of crystalline quality can also be indicated from the change of silicon lattice absorption peaks Compared with the IR spectra of sample-I, the absorption bands of ... crystal nature of sample-II is better than that of sample-I On the shoulder of ∼1085 cm− of sample-I and sample-II, there is a weak peak centered at ∼ 1200 cm− This vibrational behavior of the TO...
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Báo cáo khoa học: Chloroplast phosphoglycerate kinase from Euglena gracilis Endosymbiotic gene replacement going against the tide pdf
... Purification and cloning of Euglena chloroplast PGK Two isoforms of PGK with a molecular mass of 60 kDa were purified to electrophoretic homogeneity (Fig 1) from total Euglena gracilis cells PGK1, eluting ... and the cytosolic isoform of E gracilis, showing that cPGK of E gracilis is orthologous to both isoforms in the Kinetoplastida The second subgroup comprises the cytosolic PGKs of protozoa, fungi ... Functional conservation of calreticulin from Euglena gracilis J Euk Microbiol 45, 307–313 Yasuhira, S & Simpson, L (1997) Phylogenetic affinity of mitochondria of Euglena gracilis and kinetoplastids...
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