study of individual amino acid constituents
Báo cáo Y học: Assignment of molecular properties of a superactive coagulation factor VIIa variant to individual amino acid changes potx
Ngày tải lên :
08/03/2014, 09:20
... of a more buried N-terminal amino group [9] A majority of the present FVIIa variants exhibits an intermediate level of protection from carbamylation (Table 2) E296V-FVIIa retains about half of ... containing one or two of the mutations at positions 158{21}, 296{154} and 298{156} has enabled the identication of the amino acid changes mainly responsible for the unique prole of the triple mutant ... investigated The rate of inhibition by antithrombin reects the reactivity of the active site and has previously been found to nicely correlate to the level of amidolytic activity of FVIIa variants...
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Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt
Ngày tải lên :
18/02/2014, 13:20
... of the hcHAT proteins within the GH13 a-amylase enzyme family, as well as shed some light on the possible evolutionary events leading to separation of the heavy-chain subunit of these amino acid ... genetics of heteromeric amino acid transporters Physiology 20, 112–124 MacGregor EA, Janecek S & Svensson B (2001) Relationship of sequence and structure to specificity in the a-amylase family of enzymes ... Heteromeric amino acid transporters: biochemistry, genetics, and physiology Am J Physiol Renal Physiol 281, 995–1018 Broer S & Wagner CA (2002) Structure–function relationships of heterodimeric amino acid...
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Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf
Ngày tải lên :
22/02/2014, 07:20
... network involves the side chains of the amino acids in positions 53 and 56 in a helix B, 186 in b strand 3A, and position 334 in b strand 5A (Fig 1; the numbering of amino acids in PAI-1 is according ... combination of amino acids in these positions resulted in an accelerated latency transition Substitution of the Lys residue in position 335 counteracted the accelerating effect of some of the substitutions ... the shutter region affect the rate of latency transition by affecting the rate of passage of the RCL through the gate region Based on the aminoacid sequence of the RCL and b strand 5A being directly...
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Báo cáo khoa học: Optimization of D-amino acid oxidase for low substrate concentrations – towards a cancer enzyme therapy pdf
Ngày tải lên :
16/03/2014, 02:20
... estimation of the spectra of intermediates, of rate constants, and of the concentration of intermediates as a function of time The same program was used to simulate kinetic processes [35] Of relevance ... Role of arginine 285 in the active site of Rhodotorula gracilis d -amino acid oxidase A site-directed mutagenesis study J Biol Chem 275, 24715–24721 Massey V & Hemmerich P (1978) Photoreduction of ... spectra of flavoproteins with special reference to d -amino acid oxidase Biochemistry 4, 1161–1173 Pollegioni L, Langkau B, Tischer W, Ghisla S & Pilone MS (1993) Kinetic mechanism of d -amino acid...
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Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf
Ngày tải lên :
16/03/2014, 10:20
... hydrogen bonds with the amino group and the N3 atom of the cytosine (Fig 1) This study uses site-directed mutagenesis to further explore the contribution of these amino acids interacting with ... the protein reduces the effect of amino acid substitution on enzyme stability to almost zero This a typical example of charge rearrangement caused by an amino acid substitution In the wild-type ... from the distal point of the rest of the protein Such a dipole will have weak interactions with the rest of the protein Thus, if turned off (by removal of Arg and protonation of Asp185), the protein...
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Báo cáo khoa học: Characterization of D-amino-acid-containing excitatory conotoxins and redefinition of the I-conotoxin superfamily pot
Ngày tải lên :
16/03/2014, 22:20
... case of r11c, however, seven out of 12 amino acids at the C-terminus of r11a are not conserved, including the amino acid to be modified (Phe in the case of r11a compared with Leu in the case of ... modification, three amino acids from the C-terminus However, the I2 superfamily may lack a recognition signal, and, consequently, the same pattern of conversion of l -amino acids into d -amino acids might ... confirmed to have a d -amino acid, and synthetic or expressed versions with both l -amino acids and d -amino acids at the correct locus in order to predict when and where d -amino acids may be found...
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Báo cáo khoa học: On the reaction of D-amino acid oxidase with dioxygen: O2 diffusion pathways and enhancement of reactivity docx
Ngày tải lên :
28/03/2014, 23:20
... half-reaction of wild-type and variants of DAAO (A) Comparison of time courses of the (re)oxidation of reduced wild-type and W50 variants of DAAO followed at 455 nm upon mixing of 10 lM reduced ... position contributes to the d -amino acid preference of yeast DAAO Kinetic mechanism of DAAO variants Steady-state kinetics Dependence of the catalytic activity of DAAO variants on the oxygen ... target for the study of O2 interactions The third such residue is I225, the side chain of which, together with the side chain of M213, forms a large part of the DAAO active site roof; it is located...
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Báo cáo khoa học: "Bioavailability of the amino acid-attached prodrug as a new anti-HIV agent in rats" pdf
Ngày tải lên :
07/08/2014, 23:22
... demrofrep LA1050-PV rof alumrof eninala dica onima eht yb ytilibaliavaoib fo tnemevorpmi ehT etadidnac gurdorp wen a rof yrotcafsitas erew amsalp ni noitartnecnoc fo ecnanetniam eht dna ,mrof ... noitartsinimda laro eht roF esod eht si D dna noitartnecnoc amsalp eht si C erehw ,t · lek-pxe · dV/D = )t(C saw noitcejni suonevartni rof amsalp ni noitartnecnoc eht rof alumrof ehT noitaterpretni ... fo esod a ta noitcejni suonevartni gniwollof amsalp eht ni dnuof ylidaer saw ti ,revewoH gk/gm 001 fo etar esod a ta star ot noitartsinimda laro gniwollof amsalp eht ni detceted ton saw 2050-PV...
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Báo cáo y học: " Value of eight-amino-acid matches in predicting the allergenicity status of proteins: an empirical bioinformatic investigation" ppsx
Ngày tải lên :
13/08/2014, 13:22
... identical contiguous amino acids For proteins from 39 to 79 amino acids in length, removal of each amino acid increased the number of pairs sharing only identical contiguous amino acids by less than ... identity over 80 -amino- acid criterion were identified Protein sequences of fewer than 29 amino acids cannot show greater than 35% identity over 80 amino acids since 28 is exactly 35% of 80 Protein ... one member of a protein pair was from 29 to 79 amino acids, the cumulative number of protein pairs meeting only the eight -amino- acid criterion, but not the >35% identity over 80 -amino- acid criterion,...
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INTEGRATION OF GENERAL AMINO ACID CONTROL AND TOR REGULATORY PATHWAYS IN YEAST
Ngày tải lên :
24/08/2014, 12:49
... metabolism of amino acids This has been referred to as cross-pathway control since the induction of genes important for the biosynthesis of virtually all amino acids is independent of which amino acid ... single amino acid, such as histidine, induces the expression of genes directly involved in the synthesis of all 20 amino acids It has been confirmed for a large number of these amino acid biosynthetic ... synthesis of vitamins that are important cofactors for enzymes in pathways related to amino acids Expression of several genes encoding amino acid permeases are induced by Gcn4p 25 following amino acid...
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Development of novel amino acid derived multifunctional phosphines for enantioselective reactions
Ngày tải lên :
09/09/2015, 10:06
... FANGRUI Table of Contents Acknowledgements i Thesis Declaration iii Table of Contents iv Summary x xiii List of Tables List of Schemes xv List of Figures xx List of Abbreviations xxii List of Publications ... amino acids developed by the Lu group 61 Figure 1.7 Design of amino acids-based novel phosphine catalysts 62 Figure 2.1 Novel bifunctional phosphine catalysts based on natural primary amino acids ... DEVELOPMENT OF NOVEL AMINO ACID- DERIVED MULTIFUNCTIONAL PHOSPHINES FOR ENANTIOSELECTIVE REACTIONS ZHONG FANGRUI (B.Sc., Zhejiang Univ.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY...
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Báo cáo Y học: Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase A site-directed mutagenesis study docx
Ngày tải lên :
17/03/2014, 17:20
... contribution of residue Y238 to Table Semiquinone formation and stabilization, and redox potentials of the free forms of wild-type and Y238 mutants of D -amino acid oxidase The semiquinone form of DAAO ... Structure of D -amino acid oxidase: new insights from an old enzyme Curr Opin Struct Biol 7, 804810 Hersh, L.B & Schuman Jorns, M (1975) Use of 5-deazaFAD to study hydrogen transfer in the D -amino acid ... reaction mechanism of D -amino acid oxidase: concerted or not concerted? Bioorg Chem 16, 97110 Walsh, C.T., Schonbrunn, A & Abeles, R (1971) Studies on the mechanism of action of D -amino acid oxidase...
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DESIGN, SYNTHESIS AND STUDY OF DNA-TARGETED BENZIMIDAZOLE-AMINO ACID CONJUGATES
Ngày tải lên :
24/08/2014, 11:27
... 1.6 Plan of Study 23 v Page 1.7 List of References 25 CHAPTER DESIGN AND SYNTHESIS OF AMINO ACID- BENZIMIDAZOLEAMIDINE CONJUGATES 30 2.1 Design of Amino Acid- Benzimidazole-Amidine ... each amino acid provide subtle structural differences to the overall molecule which may lead to the targeting of different DNA sequences Individual amino acids or small chains of amino acids ... the elucidation of the structure of DNA and an understanding of the role of DNA in the life cycle of a cell The interaction of small molecules with DNA is also a prolific area of study because...
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Tài liệu THE ROLES OF AMINO ACID CHELATES IN ANIMAL NUTRITION pdf
Ngày tải lên :
15/02/2014, 16:20
... Association of Feed Control Officials, a metal amino acid chelate is lithe product resulting from the reaction of a metal ion from a soluble salt with amino acids with a mole ratio of one mole of metal ... 47 76 xx Contents EVALUATION OF THE NUTRITIONAL EFFICIENCY OF AMINO ACID CHELATES 86 Silvano Maletto and Germano Cagliero AN ASSESSMENT OF LONG TERM FEEDING OF AMINO ACID CHELATES 106 Robert B ... of a specific mineral in the feed; (b) The Roles of Amino Acid Chelates in Animal Nutrition an imbalance of another mineral or nutrient including certain vitamins, amino acids and fats, any of...
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Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants doc
Ngày tải lên :
18/02/2014, 13:20
... [14C]-labelled amino acids or [32P]-labelled tRNA Assay method Aminoacylation of transcript tRNA with [14C]-labelled amino acid Aminoacylation of [32P]-labelled transcript tRNA Arg Arg Cav Cav Source of ... comparison of amino acid utilization by the plant arginyl-tRNA synthetases The aminoacylation level attained in the presence of L-arginine was compared to that in the presence of mM of the analogue ... be aminoacylated with arginine to a level of approximately 0.05 pmol amino acid ⁄ pmol tRNA The corresponding soybean transcript had an arginine acceptance level of approximately 0.1 pmol amino...
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Tài liệu Báo cáo khoa học: Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx
Ngày tải lên :
18/02/2014, 17:20
... proline, aspartic acid, serine or alanine Simple and double FNR mutants of amino acids C266 and L268 were obtained and characterized It was observed that alteration of the amino acid volume decreases ... efficiencies of wild-type and mutant FNRs plotted as a function of different amino acid physicochemical properties The catalytic efficiencies of wild-type and mutant FNRs from Table (percentage of the ... nicotinamide of NADP+ is precisely tuned by selecting amino acids that face the other side of the tyrosine phenol ring The specific volumes of the above-mentioned residues condition the arrangement of Y308...
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Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
Ngày tải lên :
18/02/2014, 17:20
... of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues It is quite rare for such a variety of ... structure of conomarphin was similar to those of other conotoxin cDNAs The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 residues, a proregion of ... physiological role of specific Pro hydroxylation in conotoxins is still elusive 1982 The epimerized d-Phe13 is another striking feature of conomarphin The l -amino acid to d -amino acid epimerization...
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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc
Ngày tải lên :
18/02/2014, 18:20
... errors for individual amino acid contents are of the same order of magnitude as those of the total amino acid contents for glutamine (C), glutamic acid (D), asparagine (E), aspartic acid (F), ... and 21.90 1.93 (dark) The standard errors for the individual amino acid contents are of the same order of magnitude as those of the total amino acid contents Phloem exudates and xylem sap were ... 24.6 1.5 (xylem, Dark) The standard errors for individual amino acid contents are of the same order of magnitude as those of the total amino acid contents 3200 transported in the phloem, as...
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Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx
Ngày tải lên :
19/02/2014, 12:20
... the second-half reaction and are part of the active-site of CoaB; these residues may be directly involved in binding of one of the substrates, the amino acid cysteine (Fig 3) However, it cannot ... conditional lethality of the dfp-707 mutant is that one amino acid residue of the N-terminal PPC decarboxylase (CoaC) domain of Dfp is exchanged [4] For further characterization of the dfp-1 mutation, ... 4¢-phosphopantothenic acid, CTP and cysteine The synthesis of PPC occurs in two half reactions starting with the formation of 4¢-phosphopantothenoyl-CMP (activation of the carboxyl group of 4¢-phosphopantothenate)...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
Ngày tải lên :
19/02/2014, 16:20
... 3-hydroxyanthralinic acid differs from that in the pathway for 4 -amino3 -hydroxybenzoic acid The N-terminal amino acid sequence of the purified enzyme did not show significant levels of identity to sequences of 2-aminomuconate ... metacleavage pathway of 2-aminophenol in Pseudomonas sp strain AP-3 (A) Proposed pathway of 4 -amino- 3-hydroxybenzoic acid in Bordetella sp strain 10d (10) I, 4 -amino- 3hydroxybenzoic acid; II, 2 -amino- 5carboxymuconic ... (B) The reaction mixture consisted of 2.9 mL of 100 mM sodium/potassium phosphate buffer (pH 7.5), 0.1 mL of mM 4 -amino- 3-hydroxybenzoic acid, 0.1 mL of purified 4 -amino- 3-hydroxybenzoate 2,3-dioxygenase...
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