study activities methods and public meetings

THE RELEVANCE OF FORENSIC ACCOUNTING TO FINANCIAL CRIMES IN PRIVATE AND PUBLIC SECTORS OF THIRD WORLD ECONOMIES: A STUDY FROM NIGERIA potx

... agreed and for the third question, that relate to forensic accountant relevance in the public sector 81% agreed Means and standard deviations of questions two and three were 3.67 and 0.921; 3.98 and ... procedural laws, congestion and slow pace of court proceedings, jurisdiction problem and cost of investigation and prosecution In the public sector “total revenue from oil and gas will never be totally ... accountants) investigate and document financial fraud and white-collar crimes such as embezzlement and investigate allegations of fraud, estimates losses damages and assets and analyses complex financial...

12
561
0

Báo cáo y học: "A knowledge synthesis of patient and public involvement in clinical practice guidelines: study protocol" doc

Danh mục: Báo cáo khoa học

... engage patients and the public in a more meaningful and effective way The Canadian Medical Association, in its 2007 handbook on clinical practice guidelines, notes that patient and public involvement ... Partnerships and Citizen Engagement Branch is committed to ensure the effective management of public engagement activities and foster research in knowledge management, values-based decision-making, and public ... but also to the general public, which implies to consider cost effectiveness and cost impact [33,35-37] The McDonnell Norms Group suggests that response to public demand and social norms be regarded...

8
395
0

báo cáo khoa học: " A knowledge synthesis of patient and public involvement in clinical practice guidelines: study protocol" ppsx

Danh mục: Báo cáo khoa học

... engage patients and the public in a more meaningful and effective way The Canadian Medical Association, in its 2007 handbook on clinical practice guidelines, notes that patient and public involvement ... Partnerships and Citizen Engagement Branch is committed to ensure the effective management of public engagement activities and foster research in knowledge management, values-based decision-making, and public ... but also to the general public, which implies to consider cost effectiveness and cost impact [33,35-37] The McDonnell Norms Group suggests that response to public demand and social norms be regarded...

8
420
0

Study of sample preparation and pretreatment methods and their applications, in combination with high resolution chromatography

Danh mục: Cao đẳng - Đại học

... preparation methods may provide satisfied results It goes without saying that a good understanding of the chemistry involved and hands-on experiences are required to select the best isolation and preparation ... microwave assisted extraction and supercritical fluid extraction In order to understand the mechanism and advantages of different new sample preparation methods further and deeper, it is very necessary ... thesis and General Remarks about Sample Preparation Methods The thesis begins with an introductory Chapter which reviews several popular and relatively novel sample preparation methods and describes...

117
338
0

Study on native and extrinsic defects in p type zn0 semiconductors by density functional theory and experimental methods

Danh mục: Cao đẳng - Đại học

...

151
142
0

An american vietnamese cross cultural study of interrupting and asking for clarification in business meetings

Danh mục: Thạc sĩ - Cao học

... communicating and attending business meetings with American counterparts Aims and objectives of the study 3.1 Aims of the study In the light of cross-cultural pragmatics, this study aims at comparing and ... language without studying and understanding its culture Cross-cultural communication is an interesting and attractive field to find out the similarities and differences between languages and cultures ... two different languages and cultures Significance of the study Finding out the similarities and differences in interrupting and asking for clarification between Vietnamese and American entrepreneurs...

13
283
1

Glycoprotein Methods and Protocols - P1

Danh mục: Môi trường

... a UV monitor and collect fractions using a fraction collector and subject to the appropriate carbohydrate and antibody analyses 3.6 Analysis of Mucins Methods for the detection and analysis of ... antibodies and lectins or coating methods such as the glycan detection method and enzyme-linked immunosorbent assays (ELISA) All these techniques have advantages and disadvantages Solution methods ... three principally different methods are available: solution assays such as colorimetric assays for hexose and sialic acid; membrane-based methods such as slot-blotting and staining with periodic...

12
552
0

Glycoprotein Methods and Protocols - P2

Danh mục: Môi trường

... mammary gland, defined by antibodies to cytokeratins J Cell Sci 75, 17–33 Kiernan, J A (1990) Histological and Histochemical Methods: Theory and Practice, Pergamon, Oxford, UK Andrew, S M and Jasani, ... M and Milton, J D (1986) Lectin Methods and Protocols, Humana, Totowa, NJ Liener, I E., Sharon, N., and Goldstein, I J (1998) The Lectins: Properties, Functions, and Applications in Biology and ... such as Culling’s Handbook of Histopathological and Histochemical Techniques (including museum techniques) (36) or Kiernan’s Histological and Histochemical Methods: Theory and Practice (45) provide...

16
705
0

Glycoprotein Methods and Protocols - P3

Danh mục: Môi trường

... core protein specificity), and lower sensitivity than slot-blot and immunoassay methods Chap- Detection and Quantitation of Mucins 47 ter (2) discusses these assays and they are mentioned here ... greater sample volume, and superior washing) For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samples should ... NJ Nagma, K., Thornton, D J., Khan, N., and Sheehan, J K (2000) Separation and identification of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T.,...

11
572
0

Glycoprotein Methods and Protocols - P4

Danh mục: Môi trường

... (and possibly other experimental animal models), is particularly useful for validating other in vitro methods for measuring mucous thickness, for studying mucous secretion in vivo, and for studying ... and held together by bolts tightened by hand Millipore filter paper to act as a backing for the mucosa during sectioning and subsequent manipulation of the section 2.2 Histological Staining and ... (w/v) poly-L-lysine for and dried at room temperature Methods 3.1 Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections (see Notes 1–4) This is a rapid and simple method for...

8
549
0

Glycoprotein Methods and Protocols - P5

Danh mục: Môi trường

... PhosphorImager The identification of mucin precursors and mature mucins using polypeptide-specific antisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several references (1,4–11) The precursor ... Strous, G J and Dekker, J (1992) Mucin-type glycoproteins Crit Rev Biochem Mol Biol 27, 57–92 Van Klinken, B J W., Dekker, J., Büller, H A., and Einerhand, A W C (1995) Mucin gene structure and expression ... Einerhand, A W C., and Dekker, J (1995) Biosynthesis of intestinal mucins: MUC1, MUC2, MUC3, and more Biochem Soc Trans 23, 814–818 11 Van Klinken, B J W., Einerhand, A W C., Büller, H A., and Dekker,...

9
524
0

Glycoprotein Methods and Protocols - P6

Danh mục: Môi trường

... a small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer Leave to cool before pouring (hand hot) and insert well-forming comb (see ... are 1.5 mm thick and cm wide There is a compromise between band broadness and amount of sample to be loaded Larger amounts of sample tend to yield poorer quality data; i.e., the bands become more ... I., and Sheehan, J K (1996) Identification of glycoproteins on nitrocellulose membranes and gels Mol Biotechnol 5, 171–176 10 Thornton, D J., Howard, M., Devine, P L and Sheehan, J K (1995) Methods...

9
525
0

Glycoprotein Methods and Protocols - P7

Danh mục: Môi trường

... Kadler, K E., and Chapman J A (1985) Mica sandwhich technique for preparing macromolecules for rotary shadowing J Ultrastruct Res 91, 66–76 14 Kawahara K and Tanford C (1966) Viscosity and density ... (Fullerton, CA) SW 40Ti swing-out rotor, and finally unloading and analysis of the tube contents Measure out 18 mL of M GuHCl into one chamber of the gradient maker and an equal volume of M GuHCl into ... into 0.5-mL fractions, and these were analyzed for reactivity with antisera for the MUC5AC (᭹) and MUC5B (᭺) mucins The MUC5B mucin is more polydisperse than the MUC5AC mucin and has molecules of...

10
620
0

Glycoprotein Methods and Protocols - P8

Danh mục: Môi trường

... 114 Yan and Packer chromatography, and leads to a significantly lower recovery of all the amino acids, especially serine and threonine Strong acid, high temperature, and short time acid ... at least for pump and column equilibration with the initial composition of the mobile phase Figures and show typical chromatograms of the separation of amino acid standards and hydrolyzed bovine ... samples and Dr Andrew Gooley and Prof Keith Williams for their support References Yan, J X., Wilkins, M R., Ou, K., Gooley, A A., Williams, K L., Sanchez, J.-C., Golaz, O., Pasquali, C., and Hochstrasser,...

7
577
0

Glycoprotein Methods and Protocols - P9

Danh mục: Môi trường

... Notes and 8) and the length of sequence obtained is dependent on the number and distribution of proline residues Proline cleaves with reduced efficiency in Edman sequencing (Fig 1, vessel 1), and ... I., Kirk, M., Barnes, S., Lowary, T L., and Hart, G W (1996) Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by β-elimination and tandem electrospray mass spectrometry Anal ... PerSeptive Biosystems and Beckman supply this product Quantitation of glycopeptides is quite difficult, and we recommend that 10% of the sample be first sequenced using the standard program prior...

8
616
0

Glycoprotein Methods and Protocols - P10

Danh mục: Môi trường

... tandem repeat domain and overlapping each other by six amino acids (16), and the other used octamers overlapping each other by seven amino acids (17) Twelve antibodies were analyzed in total and ... must be pure and free from amines Several methods may be used to purify DMF (see Note 16) Piperidine 20% v/v: used for Fmoc deprotection Piperidine should be redistilled before use and made up ... and methanol (two times) The efficiency of the cleaning procedure should be tested by repeating steps and Absorbance levels above background indicate that antibody remains bound to the pins and...

13
737
0

Glycoprotein Methods and Protocols - P11

Danh mục: Môi trường

... lysates and media Nonspecific bands may be present and can be eliminated from consideration using the pSVL vector control lanes for comparison (Fig 2A,B, lanes and 3) The sizes of specific bands ... polymerase buffer, 2.5 mM MgCl2, 400 µM dNTPs, 200 ng of primers and or and 4, ng of template DNA (with only primers and 4), and U of Taq polymerase Perform a PCR program in a suitable thermal ... polymerase, and incubate the reaction at 72˚C to allow extension of the linked templates Add µg each of primers and 6, and continue the PCR with 30 cycles at 94˚C for 30 s, 50˚C for and 72˚C for...

13
488
0

Glycoprotein Methods and Protocols - P12

Danh mục: Môi trường

... gas Program the oven temperature from 150 to 230°C and 3°C/min and then 230 to 250°C at 5°C/min Use injector and detector temperatures of 240 and 250°C, respectively Use an injection volume of ... Nakamura, M., and Ohkura, Y (1987) Fluorometric high performance liquid chromatography of N-acetyl- and N-glycolyl neuraminic acids and its application to their microdetermination in human and animal ... monosaccharides, hexosamines, and uronic acids (see Subheading 3.3.3.2.) a Eluent 1: Deionized water b Eluent 2: 25 mM NaOH and 0.25 mM sodium acetate c Eluent 3: 200 mM NaOH and 300 mM sodium acetate...

22
619
0

Glycoprotein Methods and Protocols - P13

Danh mục: Môi trường

... (100 × cm) in a water jacket at 55°C and HPLC pump with refractive index and fluorescence detectors GlycoSepH™ and GlycoSepC™ HPLC column (Oxford GlycoSciences) Methods 3.1 Alkaline Borohydride Degradation ... 6.0, and load the sample carefully and directly to the center of the column, drop wise (see Note 3) Centrifuge for at 1100g and collect the excluded O-Glycanase O-Linked Chain Release and Fractionation ... (3 × 0.5 cm column) and elute with three column vol of HPLC-grade water Collect the effluent and eluent and then pool (2.5-mL volume) Load onto a PD10-Sephadex GM25 column and elute with HPLC-grade...

10
608
0

Glycoprotein Methods and Protocols - P14

Danh mục: Môi trường

... was introduced in the 1970s and rapidly received a large application for analyzing the sequence of N-acetyllactosamine- and oligomannosidic-type glycans Originally, and before the development ... appearence of novel methods of ionization such as fast atom bombardment ionization (FAB), electrospray ionization (ESI), and matrixassisted laser desorption ionization (MALDI) Methods 2.1 Nuclear ... method was developed by Vliegenthart and colleagues during the 1970s and essentially applied to the structure determination of N-glycans of the Nacetyllactosamine and oligomannoside type (16) More...

19
332
0