structure properties and characterization of polymeric biomaterials
Báo cáo khoa học: Genomic structure, expression and characterization of a STAT5 homologue from pufferfish (Tetraodon fluviatilis) ppt
Ngày tải lên :
31/03/2014, 07:20
... The sequences of the upper strand of the normal and the corresponding mutant oligonucleotides are listed in Table The probes were prepared by annealing the upper and lower strands of oligonucleotides, ... isolation and characterization of the STAT5 gene from T fluviatilis (TfSTAT5), the third member of the STAT family from fish, to establish the evolutionary relationship of STAT5 between mammals and teleosts ... genomic structure of TfSTAT5, the full-length cDNA was synthesized by PCR amplification using first-strand cDNA prepared from T fluviatilis gill and specific primers designed from the results of 5¢and...
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Structure, Properties, and Preparation Of Boronic Acid Derivatives Overview of Their Reactions and Applications
Ngày tải lên :
02/07/2014, 12:53
... 10 O O Me Structure, Properties, and Preparation Of Boronic Acid Derivatives Figure 1.3 Representations of the X-ray crystallographic structure of phenylboronic acid (A) ORTEP view of a dimeric ... OMe 41 42 1.2 Structure and Properties of Boronic Acid Derivatives polyols, by Kuivila and co-workers, described the preparation of several esters of phenylboronic acid by reaction of the latter, ... example, the pKa of PhB(OH)2 decreases 21 22 Structure, Properties, and Preparation Of Boronic Acid Derivatives from 8.8 to 6.8 and 4.5 upon formation of cyclic esters with glucose and fructose,...
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design, synthesis, and characterization of polymeric materials for uses in energy storage applications
Ngày tải lên :
13/11/2014, 11:13
... deprotection and lithiation of the 4-(propylcarboxalato)phenoxy side groups, and the characterization of the polymers are discussed to illustrate the dependence of ion transport and hydrophobic properties ... chemical structure of the monomer directly influences the physical properties of the polymer This allows the physical properties of polymers to be tuned for specific applications 2 1.1.1 History of ... alternating sequence of two monomers Random and alternating copolymers typically exhibit properties that are intermediate of the two respective homopolymers of each monomer The third type of copolymer...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
Ngày tải lên :
21/02/2014, 00:20
... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... suggests that the bands at approximately 110 and 37 kDa correspond to strongly attached dimers of both NrfA (a2) and NrfH (b2), respectively The presence of dithiothreitol (Fig 1A, lanes and 2) did not...
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preparation and characterization of high surface area nanosheet titania with mesoporous structure
Ngày tải lên :
20/03/2014, 13:06
... images of the nanosheet TiO2 calcined at 600 and 700 °C showed almost nanoparticles with a mixture of anatase and rutile TiO2 structure (about 10–50 nm in diameter, at 600 °C, Fig 6(g–h)), and rutile ... appeared at 600 °C and almost showed rutile TiO2 structure at 700 °C Fig 6(a–j) shows the SEM, TEM, and SAED images of the nanosheet TiO2 calcined for h at 300–700 °C The nanosheet structure after ... pattern of the as-synthesized nanosheet TiO2, and nanosheet TiO2 calcined for h at 300–700 °C The nanosheet TiO2 calcined for h at 300–500 °C consisted of anatase TiO2 structure The peak intensity of...
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Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme potx
Ngày tải lên :
23/03/2014, 20:22
... maximum of the Soret band with a small absorption coefficient (Table 1) On the other hand, relative intensities of the 498 and 630 nm bands compared with those of the 575 and 535 nm bands in the ... [23], and Hem O, N meningitidis A 2855 [25] Soret and Q (a and b) bands of the oxy form and the CO-bound form not show specific differences from those of mammalian or bacterial HOs The small band ... addition of NADPH Shift of the Soret maximum to 410 nm and appearance of the 534 and 573 nm bands in the visible region indicate that the oxy complex of heme–Syn HO-1 is produced within and accumulated...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
Ngày tải lên :
05/09/2013, 09:08
... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
Ngày tải lên :
05/09/2013, 16:11
... diameter of the PVDF membrane, the PVDF-PS membrane and the PVDF-SPS membrane is 0.25 µm, 0.12 µm and 0.16 µm, respectively It can be seen that the polymerization of styrene and the blending of PS and ... (DMAc) firstly, and then styrene, divinylbenzene (DVB) and benzoyl peroxide (BPO) were added to the solution and stirred with water bath at 343 K The dosage of PVDF, styrene, DVB and BPO was that ... International Journal of Energy and Environment (IJEE), Volume 1, Issue 4, 2010, pp.643-656 645 2.5 Measurement of proton conductivity The proton conductivity of the PVDF-SPS membrane and Nafion-117...
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Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
Ngày tải lên :
22/12/2013, 08:58
... system is capable of producing off-resonance, mirror, and flat magnetic field configurations A mirror ratio of 1·1 and a maximum flat field of 1400 G has been obtained The measured values and the design ... varying the size of the solenoid coils, thickness of iron jacket, and amp-turns (NI) The optimum values of NI for the side and middle solenoid coils were obtained to be 12950 and 8250 respectively ... μS/cm) having inlet temperature of 27◦ C Based on the length of the conductor, water pressure drop and flow, the inlet and outlet connections were provided Total five inlet and five outlet connections...
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Tài liệu Báo cáo "Preparation and characterization of nanocomposite TiO2/SnO2 films " pdf
Ngày tải lên :
13/02/2014, 04:20
... electronic structure and photocatalytic activity of the In2TiO5, Materials Science and Engineering: B Vol 139 (1) (2007) 74 J.H Kim, S.H Kim, et al., Fabrication of nanoporous and hetero structure ... steps of spray pyrolysis for TiO2 and sol-gel for SnO2 As prepared films exhibit a nanocomposite structure, constituting of a majority of TiO2 and a small amount of SnO2 located on the TiO2 grain ... concentration of the SnCl4 solution, and the films were annealed at different temperatures and for different period of time Photoconductivity measurement In order to evaluate properties of obtained...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
Ngày tải lên :
16/02/2014, 09:20
... tissue of mouse and spleen and thymus of human and mouse [11,37] Foxp3 is also highly expressed in lymphoid organs, such as the thymus and spleen [49], and its expression within a variety of other ... t-bet, stat6 and foxp3 were also determined and found to be very similar to their human homologues Both the human and zebrafish T-bet and Foxp3 genes contained the same number of exons and introns ... the expression of these genes within humans and mice, of T-bet within Ginbuna crucian carp [27] and STAT6 in mandarin fish [28] have shown similar widespread expression In the case of STAT6, highest...
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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
Ngày tải lên :
18/02/2014, 11:20
... Identification of the initiation codons in GmPDIL-3a and Gm-PDIL-3b mRNAs (A) Schematic representation of the structure of GmPDIL-3a and GmPDIL-3b mRNAs The putative ORFs (gray boxes) of GmPDIL-3a and GmPDIL-3b ... S1 Comparison of intron–exon structures of GmPDIL-3a, GmPDIL-3b and orthologs of other plants Fig S2 Multiple amino acid sequence alignment of GmPDIL-3a, GmPDIL-3b and orthologs of other plants ... http://www.phytozome.net/soybean#A) Comparison of the genomic sequences of GmPDIL-3a and GmPDIL-3b with those of the Arabidopsis and rice orthologs showed conservation of exon ⁄ intron structure across these plant...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
Ngày tải lên :
18/02/2014, 16:20
... ortholog from S mansoni Fig Structure of the SmSmad1B gene, cDNA and protein (A) Schematic representations of the SmSmad1B gene, cDNA and protein and the amino acid sequence of SmSmad1B protein Five ... properties of SmSmad1 and SmSmad1B as described above However, in the presence of SmSmad4 and the active mutant form of TbRI, both SmSmad2 and SmSmad4 interacted with GCN5, indicating the formation of ... the identification of SmSmad1B cDNA and present its gene structure along with the expression profiles, immunolocalization, and protein interaction properties Results Identification of SmSmad1B Through...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
Ngày tải lên :
18/02/2014, 16:20
... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the first strand buffer X and lL of dithiothreitol ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 for the four isoforms respectively Secondary structure of the pulchellin isoforms ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
Ngày tải lên :
19/02/2014, 06:20
... Foundation of Raisiogroup (Raisio, Finland), the Finnish Funding Agency for Technology and Innovation (TEKES) and the Commission of the European Communities, specifically RTD program ‘Quality of Life and ... bottom of a large vacant space and one of the six histidine ligands appeared to be highly flexible, was determined with a help of a caddie protein, ORF378, at ˚ 1.2–1.8 A resolution [17] Knowledge of ... Da) The identity of this peptide was then confirmed by MALDI-TOF ⁄ TOF fragment ion analysis, where partial sequences of this peptide were obtained from the ladders of b-fragment and y-fragment...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
Ngày tải lên :
19/02/2014, 07:20
... stresses used for treatment of pea seedlings were cold (A and B), salinity (C and D), wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O) Panel M is the control ... Figure 7A, D, I and L are diamidino-2phenylindole hydrochloride (DAPI) stained cells showing the nucleus, and Fig 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respectively ... ORF of 678 bp, a 5¢ UTR of 131 bp and a 3¢ UTR of 163 bp including 20 bp of poly(A) tail The PsCBL ORF encodes a protein of 225 amino acid residues with a calculated molecular mass of 25.9 kDa and...
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Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt
Ngày tải lên :
19/02/2014, 12:20
... ENDGAPS, off; NOPGAPS, off; NOHGAPS, off Graphical output of the bootstrap figure was produced by the program TREEVIEW Isolation of RNA and RT-PCR analysis Total RNA was isolated from RTG-2 cells and ... initiation cycle of 15 at 95 °C, amplification cycles of 0.5 at 94 °C, 0.5 at 50 °C and 0.5 at 72 °C, and one termination cycle of at 72 °C, with 35 cycles in total for HSF1a and HSF1b and 30 for heat ... HSF1 isoforms form both homo- and heterotrimers, there are four potential assemblies of HSF1 trimer, namely, two homotrimers (a3 and b3) and two heterotrimers (a2b1 and a1b2) The existence of the...
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Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
Ngày tải lên :
19/02/2014, 12:20
... comparison of band absorbance Absence of a sharp and convoluted spectral band around 3600–3000 cm)1 in the spectra of both chitosan and LMWC indicated the absence of free -OH groups and the involvement ... weight of GlcNAc [24] Further characterization of chitosan and the depolymerization products Further characterization of chitosan and the depolymerization products was performed using a variety of ... (moles/L) · volume of titrant (mL)/mass of sample (g) and the level of deacetylation was calculated by comparison between the number of free amino groups (per unit weight of the sample) and the equivalent...
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Tài liệu Báo cáo khoa học: Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc
Ngày tải lên :
20/02/2014, 03:20
... and characterization of GAT from wild watermelon leaves, and discusses its function during drought ⁄ strong-light stress on the basis of its two unique enzymatic properties; thermotolerance and ... (DTT) and 20% (v ⁄ v) glycerol, and 20% saturation of (NH4)2SO4 The enzyme was eluted with a linear gradient of 30–0% saturation of (NH4)2SO4 in buffer A Active fractions were collected and applied ... Gv & Kwon YM (2001) cDNA cloning of two isoforms of ornithine carbamoyltransferase from Canavalia lineata leaves and the effect of site-directed mutagenesis of the carbamoyl phosphate binding...
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![Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc](https://media.store123doc.com/images/document/14/br/vp/medium_vpv1392870032.jpg)
Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc
Ngày tải lên :
20/02/2014, 11:20
... potential, and addition of a TPP-moiety has been used to drive the selective uptake of a wide variety of Ó FEBS 2003 Fig Selective uptake of mitoDC-81 by mitochondria and subsequent alkylation of mtDNA ... mitochondria and cells In addition to facilitating the development of antigenomic therapies, such a reagent might also be useful in investigating the rate of turnover of mtDNA and in the derivation of ... the vicinity of mtDNA with the resultant binding and selective alkylation leading to a depletion of mtDNA in intact cells (Fig 1) Here we report the synthesis and characterization of a novel mitochondria-targeted...
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