... amide
oxygen atom of Gln15 and the carbonyl oxygen atoms
of Asp11 and Asn23 (Fig. 3) in an arrangement similar
to what is observed in VPRK. Both PRK and VPRK
have calcium bound at Ca3. SPRK also ... within a relatively narrow range
(pH 5–8) and are often used in the food industry.
Alkaline peptidases are generally highly active around
pH 10, have broad substrate specificity and are active
at ... subtilases is capable of accommodating at least six
amino acid residues (P4–P2¢; notation according to
Schechter and Berger [14]) ofa polypeptide substrate
or inhibitor [15], and both main chain and...
... 6,
501–523.
50 Feller G, Payan F, Theys F, Qian M, Haser R &
Gerday C (1994) Stability and structural analysis of
alpha-amylase from the antarctic psychrophile
Alteromonas haloplanctis A2 3. Eur J Biochem ... glycosylase from
Atlantic cod (Gadus morhua) reveals cold-adaptation
features. Acta Crystallogr Sect D 59, 1357–1365.
10 Aghajari N, Van Petegem F, Villeret V, Chessa JP,
Gerday C, Haser R & Van ... side chain and carbonyl oxygen of
Asp9, the side chains of Asp12, Gln13, Asp19, the car-
bonyl oxygen of Asn21 and one water molecule in a
pentagonal bipyramidal manner (Fig. 4A) . Sequence
alignments...
... Significantly, this structural feature of
NOXtp is highly similar to that of valosine-containing
protein-like ATPase from Th. acidophilum, an archaeal
member of the AAA family (ATPases associated ... the
utilization of oxygen by Desulfovibrio gigas. Eur J Bio-
chem 216, 443–448.
32 Sakuraba H, Takamatsu Y, Satomura T, Kawakami
R & Ohshima T (2001) Purification, characterization,
and application ofa ... with a native molecular
mass of 300 kDa. A ring-shaped hexameric form was revealed by electron
microscopic and image processing analyses. NOXtp catalyzed the oxidiza-
tion of NADH and NADPH and...
... cerevisiae
MAP kinase cascades typically composed of three tiers of
protein kinases, a MAP kinase (MAPK), a MAPK kinase
(MAPKK) and a MAPKK kinase (MAPKKK), are
common signalling modules in eukaryotic ... the unavoidable nonbiological variations accompanying many
experiments, it is imperative to consider a way of unravelling the functional
interaction structureofa cellular network (e.g. signalling ... cellu-
lar network structure based on stationary experimental
data, which is applicable to a network of generalized
modules under the assumption that each module con-
tains at least one intrinsic parameter...
... HinCel 6A and CcCel6C (Fig. 2)
indicated that Asp150 and Asp334 of CcCel6C are
the potential catalytic residues and could act as a
proton donor and a base, respectively. Another aspar-
tic acid ... Igarashi
3
,
Masahiro Samejima
3
, Kiyoharu Fukuda
1
, Atsushi Nishikawa
2
and Takashi Tonozuka
2
1 Department of Environmental and Natural Resource Science, Tokyo University of Agriculture and Technology, Japan
2 ... hydrolase family 6
enzyme, CcCel6C, a cellulase constitutively produced by
Coprinopsis cinerea
Yuan Liu
1
, Makoto Yoshida
1
, Yuma Kurakata
2
, Takatsugu Miyazaki
2
, Kiyohiko Igarashi
3
,
Masahiro...
... organicrinorganic
hybrid materials due to their applications in catalysis,
sorption, energy storage, molecular electronics, optical ma-
wx
terials and ceramics 1–4 . A promising synthetic route
takes advantage ofa ... synthesized and structurally characterized. The
2102 310
structure of title compound consists of infinite chains running parallel to the b axis, that are made up of distorted WO octahedra linked
6
through ... low-temperature soft approach, hy-
drothermal method, and the structure- directing function of
organoamines. Recent developments in this area have
proven its viability for the successful preparation of...
... mature a
bacterial class I c-type cytochrome, Paracoccus denitrif-
icans cytochrome c
550
[33]. Moreover, many taxa that
have heme lyase apparently have separate heme lyases
for the maturation of ... compilation ª 2009 FEBS 2831
of the alanine of the AXXCH motif (Ala25) (in
green) and the unsaturated vinyl group of the heme
(cyan) are separated by 3.41 A
˚
(as compared with a
typical thioether ... Trypanosomatidae and the
glycosomal redox balance of insect stages of Trypanoso-
ma brucei and Leishmania spp. Mol Biochem Parasitol
149, 155–169.
16 van Hellemond JJ, Simons B, Millenaar FF &
Tielens...
... Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki,
Japan
2 Research Institute of Genome-based Biofactory, National Institute ... at both ends. In the case of
Xgh7 4A, the active cleft is open. Although a precise anal-
ysis of the mode of action of Xgh7 4A has not been
performed, Xgh7 4A appears to be an endoglucanase
because ... Prism (GraphPad Software, San Diego,
CA, USA). One unit was defined as the amount of enzyme
that released 1 lmol of glucose equivalent as reducing sug-
ars from xyloglucan per minute.
Analysis of substrate...
... hydrolysis of the critical b-lactam ring and render
the antibiotic inactive against its original cellular
target, the cell wall transpeptidase. b-Lactamases of
Keywords
class C b-lactamase; cold adaptation;
psychrophile; ... enzyme activity: crys-
tallographic structure at 2 -A resolution of cephalospo-
rinase from the ampC gene of Enterobacter cloacae P99
and comparison with a class A penicillinase. Proc Natl
Acad Sci ... Lie
`
ge-Sart, Tilman, Belgium
b-Lactamases are the major causes of bacterial resis-
tance to the b-lactam family of antibiotics, such as
penicillins and cephalosporins. These enzymes catalyze
the...
... D36N-1
5’-CATCCTTCCCTATCTC
AACATCCAGCTGGTTTACT-3’
D341N-1
5’-AAGGGGAGAACTC
AACGGAACACCGGAGG-3’
D389N 5’-CACTCTCGAAGAGTTCATA
AACGAAGTGAAGAAGAATCTC-3’
5’-GAGATTCTTCTTCACTTCGT
TTATGAACTCTTCGAGAGTG-3’
M. Karlstro
¨
m ... 5’-CCTGGAGAAATCGATCA
TGAGCTTCGCTCAGTCGTG-3’
5’-CACGACTGAGCGAAGCT
CATGATCGATTTCTCCAGG-3’
F205M 5’-AAAAGGTCGACATCTGG
ATGGCGACGAAAGACACGATC-3’
5’-GATCGTGTCTTTCGTCGC
CATCCAGATGTCGACCTTTT-3’
D36N ... and 120 °C using a scan rate of 1
KÆmin
)1
. A constant pressure of 2 bar was applied in order
to avoid boiling at high temperatures. Each sample was
scanned a second time after the actual calorimetric...
... distance data by dynamical simulated
annealing from a random array of atoms. FEBS Lett
239, 129–136.
58 Yip P & Case DA (1989) A New Method for Refine-
ment of Macromolecular Structures based ... Ragona L, Molinari H, Tava A & Zetta L
(2003) Anticarcinogenic Bowman-Birk Inhibitor Isolated
from Snail Medic Seeds (Medicago scutellata): Solution
Structure and Analysis of Self Association ... respectively, by
means of standard Lineweaver–Burk analysis of initial rate
kinetics. Fitting of the experimental data required as input
data the initial concentrations of BApNA, enzyme and
inhibitor,...