structure function and dynamics with mass spectrometry
Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx
... proteins with less uniform patterns across the eight patients Proteins identified by mass spectrometry were classified by subcellular location and molecular function (Fig 3) To better understand the ... patients confidence and quantitation accuracy for proteomic research, especially for low-abundance proteins [16,17] We used iTRAQ labeling together with gel-assisted digestion and mass spectrometry ... patient were labeled with iTRAQ115 The resulting peptides from normal tissue of another patient were labeled with iTRAQ116 and peptides from tumor tissue were labeled with iTRAQ117 and incubated at...
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Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc
... R226A and R242A mutants (Table 1), all enzymes were stable for h in the presence of mM potassium phosphate and potassium sulphate Reconstitutions with PLP of apo-Cc StP and mutants thereof, and ... CD and cofactor uorescence emission spectra of native and reconstituted Table Half-lives (t1/2) of CcStP and mutants thereof in the resolution buer at 30 C and pH 7.0 Stable, no inactivation with ... Analytical enzymes and enzyme substrates were specied in previous papers [1518] All other chemicals were of reagent grade and obtained from Sigma and Fluka Preparation of apo-Cc StP and apo-Ec MalP...
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Báo cáo khoa học: Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry doc
... designated LM1 and LM2, with a mass increase of 55 and 111 Da, respectively, which within the experimental error corresponds to loading of one and two propionyl units, respectively (theoretical mass increase ... fragment also showed two peaks, LM1 and LM2 with mass increase of 67 and 137 Da (theoretical mass increase of 70 and 140 Da, respectively), which corresponds to single and double acylation by the butyryl ... polyketides and polypeptides Masses can be reliably and routinely measured with a precision of 0.01% for strong, wellresolved peaks Even with weak or overlapping peaks the precision of mass measurements...
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CHARACTERIZATION OF IMPURITIES AND DEGRADANTS USING MASS SPECTROMETRY pot
... Lee, and Guodong Chen ț Characterization of Impurities and Degradants Using Mass Spectrometry Mike S Lee and Mingshe Zhu ț Mass Spectrometry in Drug Metabolism and Disposition: Basic Principles and ... hallmark of impurity and degradant analysis HPLC is often used to profile and classify molecules and work in concert with mass spectrometry to assist with the elucidation of structure Identification ... an ion and a neutral in free space has the center-of -mass (COM) energy ECOM ECOM ¼ mi mn v mi þ mn R ð1:22Þ TANDEM MASS SPECTROMETRY 31 where mi is the ion mass, mn is the neutral mass, and n...
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Báo cáo khoa học: Serpins 2005 – fun between theb-sheets Meeting report based upon presentations made at the 4th Interna-tional Symposium on Serpin Structure, Function and Biology (Cairns, Australia) doc
... extreme environment There were many new insights into the structure and biochemistry of serpins with extra-inhibitory and cross-class inhibitory functions Guy Salvesen (The Burnham Institute, USA) ... folding, unfolding and misfolding [30] Patrick Wintrode (Case Western Reserve University, USA) presented a hydrogen exchange mass spectrometry- based approach for understanding and FEBS Journal ... The structure of PAI-1 and vitronectin has been determined [33], and it is hoped that further advances in this field will lead to a detailed structural understanding of how serpins interact with...
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Báo cáo sinh học: "Metabolite-based clustering and visualization of mass spectrometry data using one-dimensional self-organizing map" ppt
... by exact mass measurement and coelution with authentic standards Data processing The raw mass spectrometry data of all samples were processed (deconvolution, alignment, deisotoping and data reduction) ... inferred from mass spectrometry intensities Besides the intensity profile vector xi, also a particular retention time (rt) index and mass- to-charge ratio (m/z) is associated with each marker candidate ... values and a mass shift of m/z 46 The remaining marker candidates of the experiment are represented by small black dots On the right hand side the average intensity profile associated with prototype...
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Báo cáo y học: "Evaluating the potential of a novel oral lesion exudate collection method coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery" docx
... Discovery and verification of head -and- neck pre-cancer and cancer biomarkers by differential protein expression analysis using iTRAQ-labeling and multidimensional liquid chromatography and tandem mass ... for Mass Spectrometry, Martinsried, Germany [13,14] The MSM files are peaklists with high precursor mass accuracy and limited product ion ‘noise’ peaks MaxQuant achieves high precursor mass ... Discovery and verification of head -and- neck cancer biomarkers by differential protein expression analysis using iTRAQ labeling, multidimensional liquid chromatography, and tandem mass spectrometry...
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A study on structure, function and diachronic evolution of chinese extended middle construction
... Yang Lili and Wu Yayun, for their friendship and the happy days we have been together Finally, I would like to thank my parents, my sister and brother for their love, understanding, and patience ... intriguing semantic relations and syntactic features have prompted many scholars debating its particularity and intrinsic properties This study explores the structure, function, and historical developments ... appreciation to my supervisor Shi Yuzhi, with whom I had most hours of classes, independent studies and discussions He has provides me with extensive, impressive and constructive suggestions to my...
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Development and application of mass spectrometry based proteomics technologies to decipher ku70 functions
... methods with surprising sensitivity and accuracy for protein and other biomolecular analysis The true power of mass spectrometry for protein characterization is the utilization of tandem mass spectrometry ... Ku70 Protein Complex by Tandem Affinity Purification (TAP) Tag Pull Down and Mass Spectrometry 23 3.1 Introduction 3.1.1 Mass Spectrometry (MS) In the post-genome era, mass spectrometry (MS) has ... Ku70, a protein with multiple functions and can be acetylated by histone acetyltransferase The TAP purification and mass spectrometry analysis of Ku70 complex will be described and discussed in...
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Reproductive System Structure, Development and Function in Cephalopods with a New General Scale for Maturity Stages pot
... development and in reproductive strategies within coleoid cephalopods and for developing a general scale for maturity staging for males and females Materials and Methods Details of the structure ... pedal and hypobranchial glands in different species of this class In mollusks which lay their eggs in rigid capsules, capsule glands and albumin glands are present in a complex of pallial glands ... covered with oviducal gland secretions and also with a thick capsule secreted by the nidamental glands Eggs are laid one by one or in clusters in different kinds of shelters or on hard substrates and...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... incubated with Ssa1p treated with BS2G are seen in lanes and 8, and 11 and and 13, respectively Similar samples treated with BS3 are seen in lanes and 9, and 12 and and 14, respectively The arrows ... Ssa1p and Ure2p in the presence of Ssa1p with the cross-linking agents BS2G and BS3 were separated on a 7.5% acrylamide SDS ⁄ PAGE and stained with Coomassie blue (A) or western blotted and stained ... protein bands resolved by SDS ⁄ PAGE and, corresponding to monomeric Ure2p, monomeric Ssa1p and Ure2p–Ssa1p complexes with apparent molecular masses of 120 and 160 kDa were excised Each protein band...
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Báo cáo y học: "Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD-MS)" pps
... (Agilent, USA) coupled with an ion trap mass spectrometry detector was used The system was equipped with an electrospray ionization (ESI) source and an ion trap analyzer for UV and MS data acquisition ... coupled with diode array detector and mass spectrometry (HPLCDAD-ESI-MS) and to assess the quality of two commercial mycelial AC products Methods Plant Powdered mycelium and an intact fruiting body ... cytidine, and thymine [10,11,17,18]; maleic acid and succinic acid derivatives [6,19,20]; benzenoids [21]; phenol and tocopherols [8,22]; 5’-nucleotides [14]; and diterpenes [23] No chemical standardization...
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Tài liệu Cognitive function and brain structure correlations in healthy elderly East Asians doc
... correlates of age with cognition and brain measures, followed by other variables of interest with cognition and brain measures and finally the association between brain measures and performance ... regions based on prior structural and functional imaging data that related structure to cognitive functions of interest (see Greenwood, 2007; Raz, 2005; Reuter-Lorenz and Lustig, 2005 for recent reviews) ... test (Delis et al., 2001), and the Trail Making Test B (Reitan and Wolfson, 1985) Speed of processing was assessed with the TrailMaking Test A (Reitan and Wolfson, 1985) and the Symbol-Digit Modalities...
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Tài liệu Báo cáo khoa học: Structure determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory doc
... we determined the structure of E53QbsSHMT and its binary complexes with Gly, l-Ser, l-allo-Thr and ternary complex with Gly and 5-formyl-THF (FTHF) In addition, the biochemical and spectral properties ... and specific activity values for l-allo-Thr with bsSHMT were 0.8 mm and 0.65 lmolÆmg)1, respectively, and 9.5 mm and 1.1 lmolÆmg)1, respectively, with E53QbsSHMT The increase in the activity with ... was incubated with 10 mM glycine and 500 lM FTHF for and then dialyzed against buffer D not containing glycine and FTHF Such an enzyme was once again incubated with glycine (10 mM) and different...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... contribution of Met1, and Met23 substituted for Leu, Fig Comparison of the cyclization reaction as a function of temperature and pH (A), (B) and (C): (d), (s) and (.) correspond to pH 2.5, 8.0 and 11.5, ... of the hydrolysis reaction as a function of denaturing agent and pH (A), (B) and (C): (d), (s) and (.) correspond to guanidinium chloride concentrations of , and M, respectively Hydrolysis was ... incubation with each onconase: (d) (Met1)-ONC (M23L) (an onconase variant with a methionine preceding Gln1) and (s) (Pyr)-ONC (M23L) (an onconase variant with a pyroglutamyl residue at position and...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... selectin and natural killer cell receptor ligands sulfo- and sialyl-lacto-N-fucopentaoses (SuLNFPII and SLNFPII) using NMR spectroscopy and molecular dynamics simulations Comparisons with the ... pattern, sequence, and partial linkages, and assign structural details of each branch (e.g the 3- and 6-branches) Blood group H, Lea/Lex and Leb/Ley determinants, together with type and type chains, ... chain type and blood-group type (such as Lea/x and Leb/y) of underivatized oligosaccharides by negative-ion electrospray mass spectrometry (ES-MS) with collision-induced dissociation (CID) and MS/MS...
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Tài liệu Báo cáo khoa học: Second messenger function and the structure–activity relationship of cyclic adenosine diphosphoribose (cADPR) doc
... hypoxanthine) and the diphospho-bridge, as they can be replaced by much simpler ether strands These ether strands mimic the distance between the nucleobase and the diphospho-bridge, but on the other hand ... permeabilized T-cells and induced both local and global Ca2+ signalling in intact T-cells [46] 8-Azido- and 8-NH2-cIDPRE performed similarly (Fig 4) whereas the halogenated compounds 8-Br- and 8-Cl-cIDPRE ... 1.01.1 ⁄ 04 ⁄ 010, jointly with Alexander Flugel, Martinsried, Germany), the Well¨ come Trust (research collaboration grant no 068065 jointly with Barry Potter, Bath, UK) and the Deutsche Akademische...
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Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
... correlation time and an order NMR structure and dynamics of eRF1 middle domain parameter between 0.5 and 1.0, against a background of faster motions occurring with a correlation time below 20 ns and an ... A and 5.5 A The ranges for backbone torsion angles / and w were derived from the values of 13Ca, 13Cb, 13C¢, 1Ha 1HN and 15N NMR structure and dynamics of eRF1 middle domain chemical shifts and ... the most favored regions and none in the disallowed regions NMR structure and dynamics of eRF1 middle domain A B Structure analysis The conformations of the backbone and side-chains of the M...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... cyclotron resonance mass spectrometry: a primer Mass Spectrom Rev 17, 135 56 Smith RD (2000) Evolution of ESI -mass spectrometry and Fourier transform ion cyclotron resonance for proteomics and other biological ... processed with denzo and scaled with scalepack [83] The native structure of CTX [64] was used as a starting point in the model construction The initial model was rened with cns [84], and electrondensity ... using soft ionization mass spectrometry Int J Mass Spectrom 216, 127 54 Heck AJR & van den Heuvel RHH (2004) Investigation of intact protein complexes by mass spectrometry Mass Spectrom Rev 23,...
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