... bulk of AcH4-K16 in vivo is likely to be MOF in mammals and Drosophila, and its homologue Sas2 in yeast.MOF is H4-K16 specific and was originally identifiedin Drosophila as a component of the ... A (1979) Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.J Cell Biol 83, 403–427.Active chromatin and DNase I hypersensitive ... withbinding of both MOF and Tip60 (equivalent to Esa1in yeast NuA4) [124,130]. However, in human cellsdepletion of MOF, but not Tip60, results in reducedglobal levels of AcH4-K16 and defective...
... (Hamamatsu,Japan) and used in accordance with protocols approved bythe Animal Care and Use Committee of RIKEN.Plasmid construction and expressionPlasmid construction and expression in Escherichia ... full-lengthv-KIND; DKIND1, deletion of KIND1;DKIND2, deletion of KIND2; DKIND1 + 2,deletion of both KIND1 and KIND2; DRasN,deletion of RasN; DGEF, deletion of RasGEF; KIND1, KIND1 domain; KIND2,KIND2 ... α-FlagKIND2-FlagΔGEF-FlagABFig. 1. Domain structureof the MAP2-asso-ciated RasGEF v-KIND and its dendritic tar-geting via KIND2 domain. (A) Structures of the v-KIND. KIND1, KIND2, coiled-coil (CC),RasN and RasGEF domains....
... predicting,measuring and understanding the roles of different kinds of organisms in the flux and storage of elements in ecosystems. The total biomass per unit area, W,issimply the sum ofthe body mass of all ... & Jumars,1991) and the problems of dealing with extremely small particles.It is reasonable to expect tuning of the size of filtering structures and collect-ing elements to profitable prey size ... detected and the processes that appeared to be important, and (ii) to compare freshwater and marine ecosystems. In Body Size: The Structureand Function of Aquatic Ecosystems,both those questions of...
... (1990) The structureand function of the asparticproteinases. Annu. Rev. Biophys. Biophys. Chem. 19, 189–215.2. Dunn, B.M. (2002) Structureand mechanism of the pepsin-likefamily of aspartic ... and C-terminal domain in red. (B) Ribbon representation of the structureof the PSI domain of barley prophytepsin [25] (N-terminal domain,blue; C-terminal domain, red). (C) Model structureof ... isoforms by the excision of the prosegment and of most of the PSI [21]. Conversely to what has been foundin vivo [31], heavy and light chains of the processed forms of recombinant cyprosin are...
... determination of the subunitcomposition of the nAChRs involved has been hindered bythe lack of selective ligands and imperfect correlationsbetween the characteristics of native and heterologouslyexpressed ... heterologous expression [2,3]. Coexpression of a7anda8, as well as of a9 and the highly homologousa10 subunit [10] has been shown to generate heteromericchannels with properties distinct from those of ... subunits, and was not reliably expressed incombination with b4 subunits, an a6/a3 chimera consisting of the extracellular ligand-binding domain of the a6 subunit and the transmembrane and intracellular...
... [31,32,36] and their three-dimensional structures were determined [34,37–40]. Insubsequent sections, we describe the structureand dynamics of each protein, and present a comparison of s bwAFP and TmAFPwitheachotherandwithproteinsthathaveasimilar ... and TmAFPwitheachotherandwithproteinsthathaveasimilar fold. Structure of sbwAFP and TmAFPThe structureof sbwAFP has been determined by X-raycrystallography to 2.5 A˚ and by NMR at both 30 °Cand5 °C ... Overlap of X-ray structure with 5 °C NMR structure using the main chain of residues Ser12 fiThr70 in t he structure a lignment. (B) O verlap of theX-ray structure with the 30 °C NMR structure using...
... were submitted to MALDI-TOF analysis and assigned to distinct subunits.Fig. 1. SDS/PAGE and immunoblotting of S. coleoptrata hemocyanin.About 10 lg of total hemolymph protein (HL) and 3 lgofpurifiedhemocyanin ... SDS/PAGE and stained by Coomassie brilliant blue. The twohemocyanin bands were cut out and dispersed. About100 lg of hemocyanin was used for the immunization of rabbits. For determination of the ... subunits and HcX, plus 6–45 bp of the respective 5¢ untranslatedregions and the entire 3¢ untranslated regions. The standardpolyadenylation signals (AATAAA) and the poly(A)-tails of different...
... orm of b strand [18].The positive bands at » 1675 cm)1in the amide I r egion of the ROA spectra of b-andj-casein, which originate mainlyin the peptide C O stretch, are characteristic of ... theme of PPII structureand rheomor-phism is explored by a comparative ROA study, supple-mented with DSC, of caseins, synucleins and tau, togetherwith several mutants of a-synuclein and tau ... agreement between our presentresults and the earlier interpretations of the U VCD spectra of b-andj-caseins (see above) to the fact that t he basis s ets of protein UVCD spectra used in the analysis...
... model of the role of point of view in problem solving. SUMMARY We have reported here a three pronged approach to the study of problem solving action and report: I) the collected of data ... of data on problem solving and talk about problem solving, 2) development of a process model of these behaviors, and 3) use of coding techniques to extract traces of "critical phenomena" ... types of problem solving phenomena: the changes in the problem solver's organization of the problem ("point of view"), and systematic multl-utterance structures used to express...
... to grasp yet it isessential for defining precise specifications of what programs do.Like StructureandInterpretationofComputer Programs, by Abelson, Sussman,& Sussman [1, 2], our book ... ofComputer Science, and the De-partment of Microelectronics and Information Technology at KTH. We apologizeto anyone we may have inadvertently omitted.Copyrightc 2001-3 by P. Van Roy and ... improved versions of Java).The kernel language approachPractical programming languages scale up to programsof millions of lines of code.They provide a rich set of abstractions and syntax. How...
... concentrations of monomer and dimer, respectively. The Kdcanalsobeexpressed in terms of the total mass concentration of protein, [arrestin]total(in mgÆmL)1), the mass fraction of monomer, fM, and ... McDowell2, W. Clay Smith2 and Paul A. Hargrave2,31Department of Biochemistry, University of Western Ontario, London, Ontario, Canada;2Departments of Ophthalmology and 3Biochemistry and Molecular ... phosphorylation of the C-terminus of R* and binding by arrestin. Phosphorylation somewhatdecreases the ability of R* to signal transducin. Rapid shut-off of R* signalling is then accomplished by binding of arrestin...
... of the spectral pressure modes as well as the linkage between the pressure modes and hidden events of the unsteady pressure fields. 6. Synthesis and identification of random pressure field Firstly, ... applications of the Proper Transformations based on both cross spectral matrix and covariance matrix branches to analysis and identification of multi-variate random pressure fields. The random pressure ... section. The fluctuating pressure field can be represented as spatially-correlated multi-variate random processes. Understanding and knowledge of the random pressure field and its distribution is...
... minidomain and the zinc-binding domain of the zinc finger protein Ynr046w [25] (Fig. 8). The fit of the heavy atoms (Ca, C, and N) from threeb-strands and the a-helices of both the closed and theopen ... TA, Gargaro AR &Feeney J (1999) Structureand dynamics in solution of the complex of Lactobacillus casei dihydrofolate reduc-NMR structureand function of the eRF1 C-domain A. B. Mantsyzov ... variation of pH in the rangebetween 6.3 and 7.7, of ionic strength between 25 and 100 mm NaCl andof temperature between 278 and 313 K did not lead to any detectable change in thepopulations of the...
... helices AV and BVat the surface of domain V. Gly621 and Gly617 are in the area of contact with the 1095 and 2473 regions of 23S RNA. The two helicesare facing the ribosome, and the four-stranded ... 666 and 670, within one b-strand of domain V, are also important for interdomain interac-tions. The N-terminal part of the strand interacts withthe N-terminal part of helix CGin domain G, and ... occupancy of GDP in the nucleotide-bindingsite. Therefore, we present here the apo structure of S. aureus EF-G.Overall structureand comparison with previousEF-G structuresAll five domains of S....