structural magnetic and transport properties in polycrystalline manganite nd0 67sr0 33mno3 system

Identification and characterization of iron homeostasis related genes and HCC down regulated mitochondrial carrier protein (HDMCP), a novel liver specific uncoupling protein in human hepatocellular carcinoma (HCC

Ngày tải lên : 16/09/2015, 15:55

... that involved in the pathway (26) 27 2.2.2.2 Non-transferin-iron transport and uptake Besides plasma Tf, several plasma proteins including ferritin, hemopexin, haptoglobin and albumin bind iron ... vaccination program in Taiwan, showing significantly decreased incidence of HCC in parallel with a striking decline of HBV carrier rate (23) In the high incidence regions of HBV infection including ... (23, 69) In Singapore, all infants have been vaccinated since 1987, and mother-to-infant transmission has been reduced by 80% At the same time, the age-standardized incidence of HCC in Singapore...

184
496
0

$Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx$

Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx

Ngày tải lên : 30/03/2014, 20:20

... no in the NCBI protein database proteins Filamentous proteins listed in Table 1, i.e lamin B1, lamin A, lamin C, vimentin, keratin types and and actin, were all included in this group Lamins ... chromatin Another group B protein, heterogeneous nuclear ribonucleoprotein G, functions in alternative splicing [26] Interleukin enhancer-binding factor binds protein-arginine methyltransferase and ... function of this novel protein in compartments in the interchromatin space, analyses of its dynamics using GFP– ISP36 and searches for binding proteins both in vivo and in vitro are necessary as...

12
400
0

Báo cáo khoa học: Construction of a novel detection system for protein–protein interactions using yeast G-protein signaling pdf

Ngày tải lên : 16/03/2014, 01:20

... establish a rapid and reliable method for the detection of protein–protein interactions using yeast G-protein signaling In our system, protein–protein interactions were detected utilizing the knowledge ... interfering system, in which the interaction between protein X fused to Gc with the integral membrane protein Y disturbed contact of Gb with its effectors and thus inhibited G-protein signaling ... Gpa1–Gas chimeric system in which a receptor is fused to protein X and Gpa1–Gas is fused to protein Y, restoring G-protein signaling in response to the protein X–protein Y interaction [4] Subsequently,...

9
444
0

Báo cáo khoa học: hhLIM is a novel F-actin binding protein involved in actin cytoskeleton remodeling ppt

Ngày tải lên : 16/03/2014, 06:20

... their actin-binding domains To date, none of the actin-binding domain sequences registered in databases is present in hhLIM or CRPs The goals of this study were to define the actin-binding properties ... have remained obscure CRPs were first believed to interact with actin filaments in an indirect manner through the intermediation of actin-binding protein partners such as a-actinin or zyxin [10] ... lacking the C-terminal or inter-LIM domain were only weakly affected in their F-actin stabilizing and bundling activities, and trigger the formation of thick cables containing tightly packed actin...

11
347
0

Báo cáo Y học: Identiﬁcation and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is speciﬁcally modulated in thyroid cells by cAMP pot

Ngày tải lên : 23/03/2014, 21:20

... containing the p76RBE coding sequence Position of the introns in the cDNA are indicated by lines and positions in amino acids Fig Sequence comparison between human p76RBE and mouse Rhophilin [13] ... HR1-Rho-binding domain, and between amino acids 522 and 579, the PROFILE SCAN program identiﬁes a PDZ domain showing 30% identity with the PDZ domains existing in a wide variety of proteins The protein ends ... blotting in response to three main signal transduction pathways in dog thyrocytes in primary culture [thyrotropin/cAMP, EGF–HGF/MAPK (mitogen-activated protein kinase) and PMA/PKC (protein kinase...

9
394
0

Báo cáo khoa học: A novel four transmembrane spanning protein, CLP24 A hypoxically regulated cell junction protein pdf

Ngày tải lên : 30/03/2014, 14:20

... heatinactivated fetal bovine serum (Invitrogen), mM L-glutamine (Invitrogen), 100 UÆmL)1 penicillin (Invitrogen), 100 lgÆmL)1 streptomycin (Invitrogen), 10 ngÆmL)1 human recombinant EGF (Invitrogen) ... puriﬁed using spin columns and cloned (pCR II-TOPO vector, no 45-0640; Invitrogen) Following transformation and plating onto LB (Luria–Bertani) plates containing ampicillin (100 lgÆmL)1), recombinant ... reading frame of human CLP24, using cDNA from the HMEC-1 and Calu-6 human cell lines Cloning and sequencing of independent clones from both these cell lines revealed the insertion of a cytidine...

9
349
0

Báo cáo y học: "Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis" ppt

Ngày tải lên : 09/08/2014, 23:20

... CBFB: core binding factor beta; C/EBP: CCAAT/enhancerbinding protein; DMEM: Dulbecco’s modified Eagle’s medium; FABP4: fatty acid binding protein 4; GIPR: glucose-dependent insulinotropic polypeptide ... miR-30d binding site 1: RUNX2 3’-UTR Reporter (pos 229 to 325) miR-30d miR-30d binding site 2: RUNX2 3’-UTR (pos 3348 to 3354) miR-30d Reporter miR-30d binding site and miR-30a unique binding site: ... differentiation towards one lineage often inhibit differentiation towards the opposite fate Several signaling pathways, including the bone morphogenetic protein, Wnt, Hedgehog and insulin-like growth factor...

13
365
0

A novel sec7 domain containing protein BIG3 and its role in regulated secretory pathway

Ngày tải lên : 11/09/2015, 09:15

... knockdown MIN6 cell line Figure4.2 Increased betagranin storage in BIG3-deficient MIN6 cells Figure4.3 Increased insulin storage in BIG3-deficient MIN6 cells ix Figure4.4 Increased betagranin secretion ... is more predominant ( Reaven, 1993; Ferrannini, 1998; Relimpio, 2003) The balance between insulin action and insulin secretion is coordinated by insulin sensitive tissues and endocrinal beta cells ... protein recruitment distiguishes the cargo-loaded subdomains from the rest of TGN Curvature-inducing proteins, such as the Bin/amphiphysin/Rvs (BAR) domain family and the GRIP-domain-containing...

189
302
0

A novel membrane pool of protein kinase c and its role in mammalian cell signaling

Ngày tải lên : 12/09/2015, 21:10

... According to the type of amino acid phosphorylated, the protein kinases can be divided into two subgroups: protein serine/threonine kinases and protein tyrosine kinases Serine/threonine protein kinases ... called intracellular signaling proteins including G proteins, small GTPase and protein kinases13-15 5 1.2 Protein kinases 1.2.1 History and Definition The presence of phosphorus in proteins has ... serine/threonine kinases A number of residues within this domain are highly conserved between both types of protein kinases, but the tyrosine kinases and serine/threonine kinases are distinguished...

221
308
0

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

Ngày tải lên : 18/02/2014, 17:20

... GST and recombinant OMM-64 variants (rOMM-64-I-V and -C), containing different domains of the protein, to determine the calcium-binding domain of the protein (A) Schematic drawing of the recombinant ... observed by both ‘Stainsall staining’ and western blotting (arrowheads) All proteins in the EDTA-soluble (OS) and -insoluble (OI) otolith matrix were stained blue using ‘Stains-all’ In these matrices, ... contained in aggregates within the otolith matrix and identiﬁed a protein contained in the HMW protein–glycosaminoglycan aggregate that also contains the otolith structural protein otolin-1 This...

12
568
0

Báo cáo khoa học: Gc recruitment system incorporating a novel signal ampliﬁcation circuit to screen transient protein-protein interactions pot

Ngày tải lên : 05/03/2014, 23:20

... transient interacting protein pairs The aim of this study was to establish and validate a screening method for weak and transient protein–protein interactions by utilizing the Gc recruitment system ... protein–protein interactions occur in engineered a cells, they mate with intact a cells The formation of diploid cells in medium lacking methionine and lysine depends on the afﬁnities of the protein pairs ... suggesting that library screening of transient interactions is as feasible as detecting strong and stable interactions in our current system Speciﬁcity for detection of protein–protein interactions...

9
536
0

Báo cáo khoa học: The A domain of ﬁbronectin-binding protein B of Staphylococcus aureus contains a novel ﬁbronectin binding site pdf

Ngày tải lên : 06/03/2014, 00:20

... important role in the ‘docking’ step of Fg binding In order to determine whether the predicted ligandbinding trench plays a role in the interaction between the A domain of FnBPB and Fn, the binding of ... the A domain of ClfA and induce a conformational change that is incompatible with binding [12] The minimum ligand-binding site in the A domain of ClfA has been localized to subdomains N2 and N3 ... c-chain peptide binds in a hydrophobic trench lying between the N2 and N3 subdomains [13,14] ClfAs containing substitutions in residues P336 and Y338, which are located within the ligand-binding...

13
514
0

Báo cáo khóa học: SUT2 is a novel multicopy suppressor of low activity of the cAMP/protein kinase A pathway in yeast docx

Ngày tải lên : 07/03/2014, 15:20

... protein Cells were ﬁxed in 70% ethanol for DAPI staining and imaged as described in Experimental procedures Fig In uence of Sut2p on invasive growth, FLO11 expression and Ras protein level in R1278b ... RAS1 and RAS2 genes Cell 37, 437–445 Rolland, F., De Winde, J.H., Lemaire, K., Boles, E., Thevelein, J.M & Winderickx, J (2000) Glucose-induced cAMP signalling in yeast requires both a G-protein ... mm) by vortexing for 3· between 1-min intervals of cooling at °C Samples were centrifuged for at 800 g, and subsequently supernatants were assayed for protein content by determining A280 All...

8
485
0

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

Ngày tải lên : 07/03/2014, 16:20

... acetylcholinesterase (30 mM) The kinetic behavior of Rv1399c using short-chain soluble vinyl esters and triacylglycerols along with the lack of detectable activity using insoluble vinyl esters (vinyl ... inserted into the expression vector following the manufacturer’s instruction (Gateway, Invitrogen) The correct insertion of the Rv1399c ORF was conﬁrmed by DNA sequencing In our hands, protein expression ... washing Ni ± chelating Sepharose and concentration Refolding by dilution (T0) Refolding by dilution (day two) Concentration and centrifugation Desalting column and concentration After freezing...

9
584
0

Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx

Ngày tải lên : 07/03/2014, 21:20

... homologues in the context of DNA binding and chromatin regulation These homologues include members of the CpG binding protein (CGBP) family of proteins that bind DNA at unmethylated CpG islands [7], ... CGBP and DIO The GCBP proteins contain a PHD domain, followed by a DNA-binding domain (the zf-CXXC) and the newly described dPHD region (supplementary Fig S1) This family is involved in DNA binding ... the CGBP family, and seven fungal representatives (Fig 1) To root the tree, we used inhibitor of growth protein (ING3) PHD, which is another domain involved in chromatin binding and clearly more...

7
658
0

Báo cáo khoa học: The 3¢-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR ppt

Ngày tải lên : 08/03/2014, 08:20

... panel) and the UV-crosslinking results (Fig 5B) further supporting the ﬁnding that HuR is one protein binding the MARCKS 3¢-UTR in Swiss 3T3 cells In contrast to the Hu antiserum, no interaction ... the MARCKS RNA-binding proteins was observed revealing that CstF64 is not involved in formation of the RNA:protein complexes C1 and C2 (Fig 5B, lanes and 6) The addition of increasing amounts of ... and TIAR However, only three of these proteins, hnRNP D and HuR and tristetraprolin seem to in uence stability of ARE-containing mRNAs in vivo (reviewed in [51,62]) It is likely that HuR is involved...

16
754
0

Báo cáo khoa học: High levels of structural disorder in scaffold proteins as exempliﬁed by a novel neuronal protein, CASK-interactive protein1 pot

Ngày tải lên : 16/03/2014, 02:20

... directly involved in protein–protein interactions [42], these points help us to interpret the possible role of disorder in Caskin1 and in other scaffold proteins To obtain a balanced view of structural ... multidomain proteins possessing six ankyrin repeats, two sterile a motifs (SAM domains) and a single SH3 domain in the N-terminal part (cf Fig 1) In contrast, there are no recognizable domains in the ... domains of cognate proteins [31,44] Indeed, PRD of Caskin1 contains several consensus SH3 binding sites, and we postulate that it is involved in multiple interactions with other PSD proteins In...

13
408
0

Báo cáo khoa học: Identiﬁcation and functional characterization of a novel barnacle cement protein pptx

Ngày tải lên : 16/03/2014, 05:20

... squares and triangles indicate changes of resonance after protein adsorption on polycrystalline gold in ASW, on the same material in a dilute buffer containing 10 mM Tris (pH 7.4) ⁄ 25 mM NaCl, and ... In a relevant protein, the antifreeze protein, which binds to the ice nucleus to inhibit crystal growth in the cytosolic space of several organisms, including bacteria and ﬁsh [17], the Ala and ... according to the afﬁnity of the Histag fused into Mrcp-19k The cells were inoculated in LB medium [35] containing ampicillin at 37 °C for h, and transferred to freshly prepared medium, and inoculated...

11
488
0

Báo cáo khoa học: Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4) potx

Ngày tải lên : 16/03/2014, 06:20

... for 20 min, quenched in 50 mm NH4Cl in NaCl ⁄ Pi for 15 min, and permeabilized in 0.1% (w ⁄ v) Triton X-100 in NaCl ⁄ Pi for After blocking with 0.1% (w ⁄ v) gelatin in NaCl ⁄ Pi for 30 min, the ... functions and molecular evolution of the penta-EF-hand Ca2+-binding proteins Biochim Biophys Acta 1600, 51–60 Maki M & Shibata H (2007) The penta-EF-hand protein ALG-2 and its interacting ptoteins ... Chatellard-Causse C, Blot B, Cristina N, Torch S, Missotten M & Sadoul R (2002) Alix (ALG-2-interacting protein X), a protein involved in apoptosis, binds to endophilins and induces cytoplasmic vacuolization...

11
412
0

Báo cáo khóa học: Functional properties of the protein disulﬁde oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulﬁde-isomerase doc

Ngày tải lên : 16/03/2014, 16:20

... insulin disulﬁde bonds was measured at 30 °C Protein was added in mL 100 mM sodium phosphate buffer, pH 7.0, containing mM EDTA and mg bovine insulin A control cuvette contained only buffer and ... identiﬁed in both prokaryotes and eukaryotes, DsbA was only found in prokaryotes PDIs, with multiple thioredoxin/glutaredoxin domains within a single polypeptide are known in eukaryotes, and it ... units in the same protein resembles the structure of PDI; in fact, the PDI molecule possesses two thioredoxin-like domains with two active sites Interestingly, whereas thioredoxins and glutaredoxins...

12
506
0