structural dynamics of the vault ribonucleoprotein particle
Tài liệu Báo cáo khoa học: Influence of modulated structural dynamics on the kinetics of a-chymotrypsin catalysis Insights through chemical glycosylation, molecular dynamics and domain motion analysis pptx
... cutoff distance (rc ẳ 6.0 A) The dynamics of the resulting network are then dened by the Ni ã Nj Kirchhoff connectivity matrix of interresidue contacts (G) where the off diagonal elements of ... measurements of protein structural dynamics were performed to explain the observed kinetic catalytic behavior Thus, the question of whether the kinetics of a-CT catalysis are inuenced by the enzymes ... electrostatic potentials (EP) for the Ne of the lysine residues of a-CT at pH EP, EwaldEi EP is the Ewald energy of placing a charge of +1 at the location of the ith ionizable atom [89] Reactivity...
- 17
- 531
- 0
Tài liệu Banks’ exposure to interest rate risk, their earnings from term transformation, and the dynamics of the term structure pptx
... is the cross-sectional average of the bank-specific variable αi of those banks for which there is an observation in month t As the composition of this sample changes in the course of time, the ... responsible We choose the following method of mitigating the problem of changing sample composition: Instead of the exposure levels Xi (t), we investigate the change in the exposure of the same bank, ... contribution of the systematic factor and the regulation to the total timely variation of the exposure is 17.24% and, therefore, 82.76% of the variation is due to idiosyncratic effects These effects...
- 40
- 551
- 1
Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
... amount of time At the end of the heat treatment the mixtures were placed in an ice/water bath, and the enzymatic activity was stopped by lowering the pH of the reaction mixture to by addition of ... during the thermal treatment, hydrolysis of BLG was extensive, in spite of significant inactivation of the enzymes This indicates that the transient conformers originating in the course of thermal ... in these preparations could stem from the inability of the hydrolytic reaction to address all the sequential epitopes even in the denatured protein In fact, despite the apparent simplicity of the...
- 11
- 526
- 0
Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf
... 2001 cleavages of the sites were in the order: Tyr146/Asn147 ! Phe114 ! other sites The weakness of bands of fragments I and IV and the appearance of fragment II in the degradation of the Tyr146 ... in the rate of cleavage of the autolysis loop At the same time the amount of fragment III was similar to that observed in the degradation of D-chymotrypsinogen (panel a) showing that the rate of ... cleavage was the same as seen from their inactivation rates in the presence of equimolar amounts of trypsin (Table 2) The cleavage of the interdomain and autolysis loops To follow the autolysis of D-chymotrypsin...
- 9
- 613
- 0
Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... activity of the enzyme was determined using the Betamyl b-amylase test reagent (Megazyme, Wicklow, Ireland) To determine the pH stability of the b-amylase, aliquots of a solution of the purified ... for h at 25 8C Then 0.1 vol of a solution of 0.5 M sodium acetate (pH 5.0) was added and the activity of the enzyme was measured by the dinitrosalicylic acid method Inhibition of the enzyme activity ... at their 50 and 30 boundaries, respectively, and were inserted between the third letter of one codon and the first letter of the following codon Molecular modeling of the C sepium b-amylase The...
- 11
- 611
- 0
Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
... 1H,15N-HSQC spectra of the M domain of human eRF1 The numbering of the residues corresponds to that of the full eRF1 protein (A) The Gly region of the 15 H, N-HSQC spectrum of the M domain of human eRF1 ... hydrolysis at the PTC of the large ribosomal subunit [20] The three-domain structure of eRF1, with the shape of the protein resembling the letter ‘Y’, partly mimics the ‘L’-shape of the tRNA molecule, ... )42 The mean value in the family of 25 structures and the SD is no preferred conformation of the side-chain in the family 4228 v1 b There Fig The relaxation parameters of the amide 15N spin of...
- 15
- 538
- 0
Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx
... value of the mutant TNSALP was less than one-tenth of the Kcat ⁄ Km value of the soluble form of the wild-type enzyme, indicating that the replacement of valine with alanine at position 406 in the ... of TNSALP In support of our hypothesis, the cysteine residue at position 433 in one subunit of TNSALP (R433C) becomes crosslinked to the counterpart of the other subunit [29], implying that the ... containing the SV40 early promoter [11] Shortage of a precursor of GPI in the endoplasmic reticulum may be one of the reasons why a small but significant fraction of the wild-type TNSALP forms the aggregate...
- 11
- 500
- 0
Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx
... parameters, there is no relationship between S2 and the location of structural elements (Fig 4) Furthermore, the Rex terms are distributed throughout the 3D structure of the protein, and most of them ... Furthermore, the internal motions of each bond vector are independent of each other, but the rotational diffusion of the molecule affects each of those bond vectors identically [42,43] On the other ... responsible, from a thermodynamic point of view, for the binding of the monomeric species of CAC? We have previously discussed the variation in the free energy of binding as a function of the changes...
- 13
- 421
- 0
Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the figure indicate the positions of the nucleotide sequence The numbers on the left of the ... Formation of this type of oligosaccharide in BG60 has been suggested to be the result of degradative reactions, rather than imperfect biosynthesis [13] Of the plant allergens listed in the of cial ... to 230 proteins and about 65 of these are IgE binding proteins [20]; however, some of these are known to be either isoforms or the degraded products of allergens Furthermore, allergic patients...
- 10
- 665
- 0
Báo cáo khóa học: Structural properties of the protein SV-IV potx
... the total number of charges measured by electrospray MS Theoretical mass is the mass calculated on the basis of the protein amino-acid sequence The relative abundance refers to the ratio of the ... acetylation at one of the three residues, because the signal of the triacetylated species was less intense than that of the diacetylated species The MS/MS Ó FEBS 2003 Structural properties of SV-IV (Eur ... the mass increase of 42 mass units The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species From the data summarized...
- 9
- 416
- 0
Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
... for further study The concentration of the proteins was determined by the method of Lowry et al [14] and the purity was examined by SDS/PAGE [15] Assay of human PKIb activity The activity of purified ... regulation of the PKI and PKI isoforms of the inhibitor protein of the cAMP-dependent protein kinase J Biol Chem 272, 20011–20020 Byler, D.M & Susi, H (1986) Examination of the secondary structure of ... 2) The assay of its activity demonstrated that the purified PKIb inhibited the catalytic subunit of cAPK with the specific activity of 6.0 · 104 unitÆmg)1 (Fig 3A) .The Ki value determined from the...
- 6
- 531
- 0
Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
... further our knowledge with respect to the mechanisms of action of these proteins, it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the structures of these ... analysis [22] to examine in more detail the flexible characteristics of the 275–284 segment of hHR23B Because of the high quality of our 15 N-HSQC spectra, 54 of the 58 protonated backbone nitrogen ... solubilized the protein fragment in 10 mm CHAPS buffer The combination of these two A Dynamic structure of XPC-binding domain of hHR23B procedures markedly improved the quality of the 15NHSQC...
- 10
- 431
- 0
Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx
... sequences of homologs of all the six Rpt and nine Rpn subunit genes, and the partial sequences (about 80%) of the homologs of two Rpn genes were determined to deduce the amino-acid sequences of the ... homologs of genes encoding yeast 19S regulatory particle subunits A total of 24 EST clones were identified in the rice EST library based on the amino-acid sequences of the yeast RP subunits Three of the ... level based on the ratio of peak heights between them (Fig 5A) Therefore, we concluded that both translation products of these three duplicated genes were components within the RP of the rice 26S...
- 10
- 495
- 0
Báo cáo khoa học: A structural overview of the PDI family of proteins docx
... Representation of the full-length ERp57 structure with the region recognized as the x-linker colored in red (B) The N-terminal part of the x-linker folds against the b¢ domain in the structures of the ERp57 ... conformations in the structures of the isolated b¢ domain and full-length proteins In the crystal structure of the b¢x fragment of human PDI, the C-terminal part of the x-linker turns back to contact the ... that the C-terminal cap of the substrate binding domains contributes to the specificity of ERp44 How the action of the C-terminal tail of ERp44 is regulated in cells is an intriguing question Structural...
- 13
- 483
- 0
Báo cáo khoa học: Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognition pptx
... fold of one monomer is positioned above the catalytic core of the other Interestingly, Arg267 from the beginning of helix in the HTH fold of one monomer is positioned towards the active site of the ... nucleophilic attack of the a-phosphate of ATP Thus, according to the crystal structure, the specificity of serine recognition depends on: (a) the zinc ion, (b) the size of the active site and (c) the hydrophilic ... that the positioning of the cognate tRNA in the active site of mMbSerRS would be facilitated upon the conformational change of the motif loop, which was the only proximal protein region in the...
- 14
- 357
- 0
Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf
... represent the geometric centers of the cavities that are located over the protein surface, and the blue spheres indicate the geometric centers of the cavities that are located inside the b-barrel The ... indicating the importance of the positive charges for the occurrence of nonspecific contacts with DNA [22] The BPV-1 protein shows low flexibility of the loop connecting the b2 and b3 strands (Fig 6B) The ... in the dynamics of these two proteins The rmsd from the starting structures of the two proteins (supplementary Fig S1) showed, in fact, good stability over all the simulation times Also, the...
- 11
- 398
- 0
Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot
... study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the presence and the absence of MgATP ... dashed line represents the curve-fitted spectrum, i.e the dotted line is the sum of the individual components consequence of the use of D2O buffer This is the consequence of the H–D exchange that ... structure of 282 residues of a total of 344 have been solved [19] The catalytic domain of PKCh possesses a high degree of homology ( 80%) with the 3280 catalytic domain of other isoforms of PKC...
- 14
- 383
- 0
Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt
... conformation for the peptide NMR spectroscopy was further utilized to explore the structural properties of Nogo-40 The very narrow resonance dispersion of amide protons ( 0.7 p.p.m) and the lack of side-chain ... attempts to synthesize the C-terminal part of Nogo-40 failed, the peptide Nogo-24, comprising the N-terminal 24 residues of Nogo-66, was successfully produced The sequential assignment of Nogo24 was ... Interestingly, with the introduction of methanol, the CD spectra of Nogo-40 undergo dramatic changes The CD spectra of Nogo-40 in the presence of methanol at a concentration of 74% or above show one positive...
- 11
- 493
- 0
Báo cáo khoa học: Mercury(II) binding to metallothioneins Variables governing the formation and structural features of the mammalian Hg-MT species pptx
... the formation of Hg7-bMT Comparison of the three sets of CD data indicates that the degree of chirality of the Hg-bMT species is generally independent of t However, the chirality of the species ... 2004 Fig Role of the chloride anion in the degree of folding of Hg-MT species observed by comparing the CD spectra of the Hg11-MT species obtained in the titration of Zn7-MT with either HgCl2 (in ... three sets of data indicates that the stoichiometry of the species formed along the three titrations at pH depends on neither the stabilization time, t, nor the nature of the counter-ion The unique...
- 9
- 396
- 0
Báo cáo khoa học: Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 potx
... is of note that the structure of the LPS O-antigen of F columnare differs structurally from the LPS O-antigen of the fish pathogen Flavobacterium psychrophilium [21], which is a linear polymer of ... were identical with those of authentic reference glycoses, and further confirms their characterization The combined MS data and the isolation of the two aminoglycoses with the respective D-galacto ... the modified O-PS Simultaneous reduction of the carboxy function of the uronic residue C and the 4-keto function of residue B was made by treatment of the native O-PS (47 mg) in water (10 mL)...
- 7
- 405
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ đặc tuyến hiệu suất h fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25